Amino acid dipepetide frequency for bacterium HR41

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.477AlaAla: 24.477 ± 0.398
1.542AlaCys: 1.542 ± 0.063
6.693AlaAsp: 6.693 ± 0.138
8.846AlaGlu: 8.846 ± 0.179
3.978AlaPhe: 3.978 ± 0.109
12.721AlaGly: 12.721 ± 0.205
2.7AlaHis: 2.7 ± 0.084
5.264AlaIle: 5.264 ± 0.108
2.202AlaLys: 2.202 ± 0.077
17.59AlaLeu: 17.59 ± 0.298
2.178AlaMet: 2.178 ± 0.071
2.117AlaAsn: 2.117 ± 0.066
6.88AlaPro: 6.88 ± 0.148
3.82AlaGln: 3.82 ± 0.102
16.249AlaArg: 16.249 ± 0.227
7.073AlaSer: 7.073 ± 0.166
7.064AlaThr: 7.064 ± 0.13
12.365AlaVal: 12.365 ± 0.205
1.542AlaTrp: 1.542 ± 0.062
2.103AlaTyr: 2.103 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
1.549CysAla: 1.549 ± 0.066
0.122CysCys: 0.122 ± 0.015
0.758CysAsp: 0.758 ± 0.042
0.806CysGlu: 0.806 ± 0.04
0.256CysPhe: 0.256 ± 0.026
1.276CysGly: 1.276 ± 0.057
0.186CysHis: 0.186 ± 0.02
0.238CysIle: 0.238 ± 0.024
0.125CysLys: 0.125 ± 0.017
0.804CysLeu: 0.804 ± 0.042
0.111CysMet: 0.111 ± 0.016
0.133CysAsn: 0.133 ± 0.018
0.609CysPro: 0.609 ± 0.04
0.192CysGln: 0.192 ± 0.02
0.939CysArg: 0.939 ± 0.054
0.507CysSer: 0.507 ± 0.036
0.374CysThr: 0.374 ± 0.028
1.014CysVal: 1.014 ± 0.044
0.087CysTrp: 0.087 ± 0.014
0.183CysTyr: 0.183 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
6.638AspAla: 6.638 ± 0.109
0.419AspCys: 0.419 ± 0.033
3.174AspAsp: 3.174 ± 0.097
4.486AspGlu: 4.486 ± 0.112
1.509AspPhe: 1.509 ± 0.065
5.094AspGly: 5.094 ± 0.119
1.254AspHis: 1.254 ± 0.057
1.691AspIle: 1.691 ± 0.064
0.747AspLys: 0.747 ± 0.041
5.528AspLeu: 5.528 ± 0.123
0.505AspMet: 0.505 ± 0.033
0.692AspAsn: 0.692 ± 0.04
4.13AspPro: 4.13 ± 0.087
1.284AspGln: 1.284 ± 0.065
5.723AspArg: 5.723 ± 0.131
2.042AspSer: 2.042 ± 0.067
2.088AspThr: 2.088 ± 0.067
4.242AspVal: 4.242 ± 0.11
0.601AspTrp: 0.601 ± 0.038
0.987AspTyr: 0.987 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
7.573GluAla: 7.573 ± 0.151
0.465GluCys: 0.465 ± 0.033
2.16GluAsp: 2.16 ± 0.084
4.211GluGlu: 4.211 ± 0.121
1.699GluPhe: 1.699 ± 0.056
4.377GluGly: 4.377 ± 0.111
1.693GluHis: 1.693 ± 0.075
2.942GluIle: 2.942 ± 0.099
1.151GluLys: 1.151 ± 0.062
9.967GluLeu: 9.967 ± 0.193
0.837GluMet: 0.837 ± 0.048
0.979GluAsn: 0.979 ± 0.046
3.847GluPro: 3.847 ± 0.099
2.689GluGln: 2.689 ± 0.093
11.463GluArg: 11.463 ± 0.21
2.392GluSer: 2.392 ± 0.075
2.453GluThr: 2.453 ± 0.079
4.936GluVal: 4.936 ± 0.125
0.712GluTrp: 0.712 ± 0.041
0.876GluTyr: 0.876 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
4.792PheAla: 4.792 ± 0.121
0.389PheCys: 0.389 ± 0.029
2.121PheAsp: 2.121 ± 0.063
2.101PheGlu: 2.101 ± 0.075
0.97PhePhe: 0.97 ± 0.044
3.025PheGly: 3.025 ± 0.082
0.498PheHis: 0.498 ± 0.033
0.758PheIle: 0.758 ± 0.039
0.487PheLys: 0.487 ± 0.033
2.429PheLeu: 2.429 ± 0.077
0.339PheMet: 0.339 ± 0.03
0.526PheAsn: 0.526 ± 0.032
1.328PhePro: 1.328 ± 0.055
0.647PheGln: 0.647 ± 0.038
2.202PheArg: 2.202 ± 0.062
1.295PheSer: 1.295 ± 0.055
1.254PheThr: 1.254 ± 0.049
2.7PheVal: 2.7 ± 0.084
0.339PheTrp: 0.339 ± 0.03
0.603PheTyr: 0.603 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
11.542GlyAla: 11.542 ± 0.176
1.0GlyCys: 1.0 ± 0.051
4.786GlyAsp: 4.786 ± 0.107
6.391GlyGlu: 6.391 ± 0.133
2.665GlyPhe: 2.665 ± 0.067
8.438GlyGly: 8.438 ± 0.16
1.736GlyHis: 1.736 ± 0.06
3.303GlyIle: 3.303 ± 0.091
1.881GlyLys: 1.881 ± 0.067
7.894GlyLeu: 7.894 ± 0.159
1.483GlyMet: 1.483 ± 0.051
1.474GlyAsn: 1.474 ± 0.064
3.951GlyPro: 3.951 ± 0.086
2.165GlyGln: 2.165 ± 0.08
9.425GlyArg: 9.425 ± 0.148
5.085GlySer: 5.085 ± 0.104
4.513GlyThr: 4.513 ± 0.118
7.394GlyVal: 7.394 ± 0.127
1.184GlyTrp: 1.184 ± 0.055
1.852GlyTyr: 1.852 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
2.51HisAla: 2.51 ± 0.079
0.232HisCys: 0.232 ± 0.022
1.129HisAsp: 1.129 ± 0.053
1.324HisGlu: 1.324 ± 0.057
0.588HisPhe: 0.588 ± 0.039
1.986HisGly: 1.986 ± 0.064
0.614HisHis: 0.614 ± 0.041
0.548HisIle: 0.548 ± 0.034
0.319HisLys: 0.319 ± 0.026
1.946HisLeu: 1.946 ± 0.076
0.277HisMet: 0.277 ± 0.023
0.317HisAsn: 0.317 ± 0.025
1.455HisPro: 1.455 ± 0.064
0.505HisGln: 0.505 ± 0.04
1.931HisArg: 1.931 ± 0.09
0.819HisSer: 0.819 ± 0.041
0.845HisThr: 0.845 ± 0.04
1.699HisVal: 1.699 ± 0.06
0.249HisTrp: 0.249 ± 0.022
0.411HisTyr: 0.411 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.011IleAla: 6.011 ± 0.123
0.404IleCys: 0.404 ± 0.03
2.737IleAsp: 2.737 ± 0.088
3.097IleGlu: 3.097 ± 0.104
0.845IlePhe: 0.845 ± 0.04
3.311IleGly: 3.311 ± 0.107
0.57IleHis: 0.57 ± 0.039
0.712IleIle: 0.712 ± 0.05
0.671IleLys: 0.671 ± 0.044
2.304IleLeu: 2.304 ± 0.083
0.31IleMet: 0.31 ± 0.026
0.607IleAsn: 0.607 ± 0.038
1.623IlePro: 1.623 ± 0.055
0.629IleGln: 0.629 ± 0.038
2.311IleArg: 2.311 ± 0.079
1.418IleSer: 1.418 ± 0.058
1.492IleThr: 1.492 ± 0.061
3.766IleVal: 3.766 ± 0.098
0.336IleTrp: 0.336 ± 0.027
0.644IleTyr: 0.644 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
1.892LysAla: 1.892 ± 0.076
0.09LysCys: 0.09 ± 0.016
0.581LysAsp: 0.581 ± 0.043
0.891LysGlu: 0.891 ± 0.054
0.376LysPhe: 0.376 ± 0.03
1.204LysGly: 1.204 ± 0.053
0.339LysHis: 0.339 ± 0.034
0.817LysIle: 0.817 ± 0.048
0.391LysLys: 0.391 ± 0.032
2.171LysLeu: 2.171 ± 0.069
0.266LysMet: 0.266 ± 0.024
0.299LysAsn: 0.299 ± 0.026
1.164LysPro: 1.164 ± 0.061
0.603LysGln: 0.603 ± 0.043
2.071LysArg: 2.071 ± 0.072
0.703LysSer: 0.703 ± 0.043
0.83LysThr: 0.83 ± 0.046
1.472LysVal: 1.472 ± 0.063
0.166LysTrp: 0.166 ± 0.021
0.288LysTyr: 0.288 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
19.726LeuAla: 19.726 ± 0.315
1.153LeuCys: 1.153 ± 0.049
5.972LeuAsp: 5.972 ± 0.131
7.464LeuGlu: 7.464 ± 0.154
2.875LeuPhe: 2.875 ± 0.1
9.39LeuGly: 9.39 ± 0.153
1.688LeuHis: 1.688 ± 0.063
2.728LeuIle: 2.728 ± 0.086
1.418LeuLys: 1.418 ± 0.066
10.45LeuLeu: 10.45 ± 0.2
1.077LeuMet: 1.077 ± 0.044
1.232LeuAsn: 1.232 ± 0.042
6.31LeuPro: 6.31 ± 0.124
1.957LeuGln: 1.957 ± 0.076
11.12LeuArg: 11.12 ± 0.187
4.141LeuSer: 4.141 ± 0.125
4.039LeuThr: 4.039 ± 0.104
10.679LeuVal: 10.679 ± 0.188
1.201LeuTrp: 1.201 ± 0.06
1.706LeuTyr: 1.706 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
1.833MetAla: 1.833 ± 0.056
0.098MetCys: 0.098 ± 0.014
0.459MetAsp: 0.459 ± 0.029
0.592MetGlu: 0.592 ± 0.038
0.389MetPhe: 0.389 ± 0.03
0.963MetGly: 0.963 ± 0.05
0.284MetHis: 0.284 ± 0.025
0.533MetIle: 0.533 ± 0.033
0.312MetLys: 0.312 ± 0.026
1.433MetLeu: 1.433 ± 0.051
0.181MetMet: 0.181 ± 0.021
0.315MetAsn: 0.315 ± 0.024
1.007MetPro: 1.007 ± 0.046
0.402MetGln: 0.402 ± 0.032
1.553MetArg: 1.553 ± 0.06
0.935MetSer: 0.935 ± 0.045
0.782MetThr: 0.782 ± 0.043
1.077MetVal: 1.077 ± 0.051
0.131MetTrp: 0.131 ± 0.017
0.19MetTyr: 0.19 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.073AsnAla: 2.073 ± 0.066
0.155AsnCys: 0.155 ± 0.019
0.819AsnAsp: 0.819 ± 0.05
0.92AsnGlu: 0.92 ± 0.045
0.483AsnPhe: 0.483 ± 0.035
1.47AsnGly: 1.47 ± 0.06
0.315AsnHis: 0.315 ± 0.023
0.59AsnIle: 0.59 ± 0.038
0.28AsnLys: 0.28 ± 0.024
1.492AsnLeu: 1.492 ± 0.057
0.227AsnMet: 0.227 ± 0.022
0.286AsnAsn: 0.286 ± 0.03
1.228AsnPro: 1.228 ± 0.058
0.365AsnGln: 0.365 ± 0.029
1.352AsnArg: 1.352 ± 0.056
0.633AsnSer: 0.633 ± 0.039
0.699AsnThr: 0.699 ± 0.037
1.446AsnVal: 1.446 ± 0.05
0.208AsnTrp: 0.208 ± 0.022
0.328AsnTyr: 0.328 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
7.745ProAla: 7.745 ± 0.151
0.509ProCys: 0.509 ± 0.03
3.6ProAsp: 3.6 ± 0.097
3.77ProGlu: 3.77 ± 0.096
1.721ProPhe: 1.721 ± 0.07
5.402ProGly: 5.402 ± 0.1
1.225ProHis: 1.225 ± 0.051
1.741ProIle: 1.741 ± 0.067
0.834ProLys: 0.834 ± 0.051
6.317ProLeu: 6.317 ± 0.145
0.642ProMet: 0.642 ± 0.039
1.005ProAsn: 1.005 ± 0.044
4.067ProPro: 4.067 ± 0.124
1.64ProGln: 1.64 ± 0.07
5.483ProArg: 5.483 ± 0.123
2.864ProSer: 2.864 ± 0.089
2.844ProThr: 2.844 ± 0.093
4.408ProVal: 4.408 ± 0.113
0.764ProTrp: 0.764 ± 0.047
1.044ProTyr: 1.044 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
3.429GlnAla: 3.429 ± 0.098
0.142GlnCys: 0.142 ± 0.019
0.791GlnAsp: 0.791 ± 0.045
1.247GlnGlu: 1.247 ± 0.055
0.592GlnPhe: 0.592 ± 0.04
1.975GlnGly: 1.975 ± 0.067
0.588GlnHis: 0.588 ± 0.038
0.983GlnIle: 0.983 ± 0.057
0.481GlnLys: 0.481 ± 0.033
3.445GlnLeu: 3.445 ± 0.096
0.384GlnMet: 0.384 ± 0.032
0.408GlnAsn: 0.408 ± 0.032
1.736GlnPro: 1.736 ± 0.061
0.926GlnGln: 0.926 ± 0.061
3.595GlnArg: 3.595 ± 0.101
0.898GlnSer: 0.898 ± 0.047
1.182GlnThr: 1.182 ± 0.059
2.073GlnVal: 2.073 ± 0.073
0.304GlnTrp: 0.304 ± 0.027
0.325GlnTyr: 0.325 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
15.124ArgAla: 15.124 ± 0.239
1.249ArgCys: 1.249 ± 0.059
5.566ArgAsp: 5.566 ± 0.108
7.697ArgGlu: 7.697 ± 0.166
3.571ArgPhe: 3.571 ± 0.083
7.863ArgGly: 7.863 ± 0.153
2.169ArgHis: 2.169 ± 0.09
3.914ArgIle: 3.914 ± 0.089
1.927ArgLys: 1.927 ± 0.071
11.166ArgLeu: 11.166 ± 0.152
1.795ArgMet: 1.795 ± 0.057
1.577ArgAsn: 1.577 ± 0.071
6.044ArgPro: 6.044 ± 0.125
2.857ArgGln: 2.857 ± 0.08
12.457ArgArg: 12.457 ± 0.229
5.935ArgSer: 5.935 ± 0.128
4.991ArgThr: 4.991 ± 0.101
8.604ArgVal: 8.604 ± 0.157
1.503ArgTrp: 1.503 ± 0.059
2.309ArgTyr: 2.309 ± 0.071
0.0ArgXaa: 0.0 ± 0.0
Ser
6.494SerAla: 6.494 ± 0.145
0.529SerCys: 0.529 ± 0.034
2.833SerAsp: 2.833 ± 0.078
3.054SerGlu: 3.054 ± 0.086
1.407SerPhe: 1.407 ± 0.056
5.321SerGly: 5.321 ± 0.109
0.789SerHis: 0.789 ± 0.037
1.324SerIle: 1.324 ± 0.064
0.723SerLys: 0.723 ± 0.041
4.517SerLeu: 4.517 ± 0.115
0.823SerMet: 0.823 ± 0.04
0.721SerAsn: 0.721 ± 0.036
2.805SerPro: 2.805 ± 0.101
1.149SerGln: 1.149 ± 0.047
4.117SerArg: 4.117 ± 0.091
2.532SerSer: 2.532 ± 0.098
2.117SerThr: 2.117 ± 0.072
3.41SerVal: 3.41 ± 0.092
0.583SerTrp: 0.583 ± 0.034
0.852SerTyr: 0.852 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.179ThrAla: 6.179 ± 0.126
0.387ThrCys: 0.387 ± 0.026
2.01ThrAsp: 2.01 ± 0.073
2.114ThrGlu: 2.114 ± 0.068
1.485ThrPhe: 1.485 ± 0.059
4.46ThrGly: 4.46 ± 0.102
0.806ThrHis: 0.806 ± 0.04
1.951ThrIle: 1.951 ± 0.069
0.806ThrLys: 0.806 ± 0.04
5.146ThrLeu: 5.146 ± 0.102
0.647ThrMet: 0.647 ± 0.039
0.808ThrAsn: 0.808 ± 0.041
2.962ThrPro: 2.962 ± 0.091
0.963ThrGln: 0.963 ± 0.046
3.737ThrArg: 3.737 ± 0.091
2.176ThrSer: 2.176 ± 0.067
2.427ThrThr: 2.427 ± 0.071
4.893ThrVal: 4.893 ± 0.118
0.435ThrTrp: 0.435 ± 0.031
0.885ThrTyr: 0.885 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
14.44ValAla: 14.44 ± 0.233
1.011ValCys: 1.011 ± 0.051
5.144ValAsp: 5.144 ± 0.125
6.459ValGlu: 6.459 ± 0.139
2.359ValPhe: 2.359 ± 0.081
7.503ValGly: 7.503 ± 0.139
1.601ValHis: 1.601 ± 0.056
2.811ValIle: 2.811 ± 0.101
1.343ValLys: 1.343 ± 0.062
8.305ValLeu: 8.305 ± 0.157
0.987ValMet: 0.987 ± 0.046
1.3ValAsn: 1.3 ± 0.057
4.88ValPro: 4.88 ± 0.102
1.804ValGln: 1.804 ± 0.068
8.897ValArg: 8.897 ± 0.135
3.399ValSer: 3.399 ± 0.076
4.043ValThr: 4.043 ± 0.095
8.945ValVal: 8.945 ± 0.156
0.965ValTrp: 0.965 ± 0.046
1.383ValTyr: 1.383 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
1.302TrpAla: 1.302 ± 0.055
0.135TrpCys: 0.135 ± 0.018
0.461TrpAsp: 0.461 ± 0.03
0.627TrpGlu: 0.627 ± 0.038
0.426TrpPhe: 0.426 ± 0.034
0.71TrpGly: 0.71 ± 0.039
0.304TrpHis: 0.304 ± 0.023
0.457TrpIle: 0.457 ± 0.034
0.168TrpLys: 0.168 ± 0.019
1.57TrpLeu: 1.57 ± 0.07
0.183TrpMet: 0.183 ± 0.021
0.26TrpAsn: 0.26 ± 0.022
0.609TrpPro: 0.609 ± 0.041
0.483TrpGln: 0.483 ± 0.037
1.673TrpArg: 1.673 ± 0.064
0.55TrpSer: 0.55 ± 0.037
0.465TrpThr: 0.465 ± 0.03
0.9TrpVal: 0.9 ± 0.043
0.21TrpTrp: 0.21 ± 0.023
0.264TrpTyr: 0.264 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.075TyrAla: 2.075 ± 0.072
0.236TyrCys: 0.236 ± 0.024
1.044TyrAsp: 1.044 ± 0.043
1.324TyrGlu: 1.324 ± 0.059
0.609TyrPhe: 0.609 ± 0.039
1.673TyrGly: 1.673 ± 0.059
0.345TyrHis: 0.345 ± 0.027
0.496TyrIle: 0.496 ± 0.038
0.349TyrLys: 0.349 ± 0.026
1.852TyrLeu: 1.852 ± 0.063
0.212TyrMet: 0.212 ± 0.021
0.31TyrAsn: 0.31 ± 0.028
0.989TyrPro: 0.989 ± 0.053
0.424TyrGln: 0.424 ± 0.03
2.023TyrArg: 2.023 ± 0.069
0.701TyrSer: 0.701 ± 0.038
0.775TyrThr: 0.775 ± 0.046
1.557TyrVal: 1.557 ± 0.062
0.238TyrTrp: 0.238 ± 0.024
0.441TyrTyr: 0.441 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1586 proteins (457819 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski