Amino acid dipepetide frequency for Ruminococcaceae bacterium TF06-43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.251AlaAla: 11.251 ± 0.152	1.5AlaCys: 1.5 ± 0.047	5.369AlaAsp: 5.369 ± 0.08	6.719AlaGlu: 6.719 ± 0.087	3.437AlaPhe: 3.437 ± 0.07	6.299AlaGly: 6.299 ± 0.088	1.624AlaHis: 1.624 ± 0.04	5.314AlaIle: 5.314 ± 0.076	4.871AlaLys: 4.871 ± 0.091	9.949AlaLeu: 9.949 ± 0.139	2.857AlaMet: 2.857 ± 0.056	2.664AlaAsn: 2.664 ± 0.062	3.233AlaPro: 3.233 ± 0.07	3.606AlaGln: 3.606 ± 0.068	4.581AlaArg: 4.581 ± 0.086	4.628AlaSer: 4.628 ± 0.075	3.735AlaThr: 3.735 ± 0.071	7.325AlaVal: 7.325 ± 0.097	0.8AlaTrp: 0.8 ± 0.029	3.07AlaTyr: 3.07 ± 0.057	0.001AlaXaa: 0.001 ± 0.001
Cys	1.74CysAla: 1.74 ± 0.049	0.355CysCys: 0.355 ± 0.022	0.974CysAsp: 0.974 ± 0.036	1.091CysGlu: 1.091 ± 0.034	0.679CysPhe: 0.679 ± 0.028	1.853CysGly: 1.853 ± 0.05	0.35CysHis: 0.35 ± 0.02	0.953CysIle: 0.953 ± 0.032	0.835CysLys: 0.835 ± 0.032	1.293CysLeu: 1.293 ± 0.04	0.392CysMet: 0.392 ± 0.021	0.506CysAsn: 0.506 ± 0.024	0.839CysPro: 0.839 ± 0.037	0.407CysGln: 0.407 ± 0.023	1.073CysArg: 1.073 ± 0.038	0.97CysSer: 0.97 ± 0.034	0.995CysThr: 0.995 ± 0.039	1.194CysVal: 1.194 ± 0.04	0.149CysTrp: 0.149 ± 0.012	0.57CysTyr: 0.57 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	5.511AspAla: 5.511 ± 0.093	0.911AspCys: 0.911 ± 0.034	3.248AspAsp: 3.248 ± 0.067	4.838AspGlu: 4.838 ± 0.076	2.606AspPhe: 2.606 ± 0.054	5.101AspGly: 5.101 ± 0.089	1.006AspHis: 1.006 ± 0.034	3.528AspIle: 3.528 ± 0.07	3.016AspLys: 3.016 ± 0.069	4.965AspLeu: 4.965 ± 0.084	1.669AspMet: 1.669 ± 0.043	1.839AspAsn: 1.839 ± 0.047	2.064AspPro: 2.064 ± 0.052	1.161AspGln: 1.161 ± 0.036	2.815AspArg: 2.815 ± 0.064	2.928AspSer: 2.928 ± 0.06	3.152AspThr: 3.152 ± 0.058	3.821AspVal: 3.821 ± 0.063	0.718AspTrp: 0.718 ± 0.03	2.533AspTyr: 2.533 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	5.849GluAla: 5.849 ± 0.084	0.908GluCys: 0.908 ± 0.03	3.887GluAsp: 3.887 ± 0.066	5.27GluGlu: 5.27 ± 0.088	2.283GluPhe: 2.283 ± 0.047	3.963GluGly: 3.963 ± 0.071	1.561GluHis: 1.561 ± 0.044	4.334GluIle: 4.334 ± 0.066	5.413GluLys: 5.413 ± 0.089	7.123GluLeu: 7.123 ± 0.094	2.115GluMet: 2.115 ± 0.057	3.497GluAsn: 3.497 ± 0.064	2.062GluPro: 2.062 ± 0.05	3.355GluGln: 3.355 ± 0.06	4.456GluArg: 4.456 ± 0.077	3.306GluSer: 3.306 ± 0.059	3.72GluThr: 3.72 ± 0.068	4.114GluVal: 4.114 ± 0.075	0.654GluTrp: 0.654 ± 0.026	2.497GluTyr: 2.497 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.626PheAla: 3.626 ± 0.065	0.863PheCys: 0.863 ± 0.031	2.613PheAsp: 2.613 ± 0.058	2.344PheGlu: 2.344 ± 0.046	1.708PhePhe: 1.708 ± 0.052	2.885PheGly: 2.885 ± 0.056	0.824PheHis: 0.824 ± 0.026	2.076PheIle: 2.076 ± 0.057	1.465PheLys: 1.465 ± 0.044	3.936PheLeu: 3.936 ± 0.079	0.876PheMet: 0.876 ± 0.033	1.244PheAsn: 1.244 ± 0.037	1.495PhePro: 1.495 ± 0.041	1.032PheGln: 1.032 ± 0.033	1.979PheArg: 1.979 ± 0.052	2.905PheSer: 2.905 ± 0.056	2.355PheThr: 2.355 ± 0.053	2.583PheVal: 2.583 ± 0.049	0.409PheTrp: 0.409 ± 0.021	1.515PheTyr: 1.515 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	5.9GlyAla: 5.9 ± 0.091	1.354GlyCys: 1.354 ± 0.036	3.76GlyAsp: 3.76 ± 0.073	5.044GlyGlu: 5.044 ± 0.078	2.901GlyPhe: 2.901 ± 0.057	5.658GlyGly: 5.658 ± 0.103	1.452GlyHis: 1.452 ± 0.044	4.751GlyIle: 4.751 ± 0.087	5.087GlyLys: 5.087 ± 0.08	6.069GlyLeu: 6.069 ± 0.086	2.248GlyMet: 2.248 ± 0.052	2.66GlyAsn: 2.66 ± 0.06	1.18GlyPro: 1.18 ± 0.037	2.081GlyGln: 2.081 ± 0.052	3.659GlyArg: 3.659 ± 0.064	4.227GlySer: 4.227 ± 0.09	4.579GlyThr: 4.579 ± 0.094	5.48GlyVal: 5.48 ± 0.084	0.859GlyTrp: 0.859 ± 0.036	3.062GlyTyr: 3.062 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.523HisAla: 1.523 ± 0.039	0.444HisCys: 0.444 ± 0.024	1.119HisAsp: 1.119 ± 0.043	1.359HisGlu: 1.359 ± 0.043	0.899HisPhe: 0.899 ± 0.031	1.459HisGly: 1.459 ± 0.04	0.472HisHis: 0.472 ± 0.03	1.261HisIle: 1.261 ± 0.045	0.885HisLys: 0.885 ± 0.027	1.761HisLeu: 1.761 ± 0.051	0.464HisMet: 0.464 ± 0.021	0.668HisAsn: 0.668 ± 0.026	1.037HisPro: 1.037 ± 0.035	0.514HisGln: 0.514 ± 0.022	1.109HisArg: 1.109 ± 0.038	1.091HisSer: 1.091 ± 0.032	1.121HisThr: 1.121 ± 0.034	1.191HisVal: 1.191 ± 0.037	0.21HisTrp: 0.21 ± 0.014	0.77HisTyr: 0.77 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.869IleAla: 5.869 ± 0.083	1.154IleCys: 1.154 ± 0.039	3.647IleAsp: 3.647 ± 0.064	3.815IleGlu: 3.815 ± 0.071	2.208IlePhe: 2.208 ± 0.052	4.352IleGly: 4.352 ± 0.075	1.094IleHis: 1.094 ± 0.036	3.21IleIle: 3.21 ± 0.069	2.646IleLys: 2.646 ± 0.063	5.596IleLeu: 5.596 ± 0.09	1.373IleMet: 1.373 ± 0.038	1.887IleAsn: 1.887 ± 0.046	2.83IlePro: 2.83 ± 0.066	1.605IleGln: 1.605 ± 0.041	3.393IleArg: 3.393 ± 0.065	3.888IleSer: 3.888 ± 0.074	3.481IleThr: 3.481 ± 0.07	4.463IleVal: 4.463 ± 0.082	0.517IleTrp: 0.517 ± 0.024	2.115IleTyr: 2.115 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.943LysAla: 4.943 ± 0.077	0.715LysCys: 0.715 ± 0.029	2.948LysAsp: 2.948 ± 0.067	4.151LysGlu: 4.151 ± 0.08	1.509LysPhe: 1.509 ± 0.039	3.373LysGly: 3.373 ± 0.069	0.985LysHis: 0.985 ± 0.036	3.16LysIle: 3.16 ± 0.065	4.467LysLys: 4.467 ± 0.09	5.182LysLeu: 5.182 ± 0.078	1.686LysMet: 1.686 ± 0.042	2.405LysAsn: 2.405 ± 0.059	2.141LysPro: 2.141 ± 0.046	2.067LysGln: 2.067 ± 0.054	3.292LysArg: 3.292 ± 0.06	3.162LysSer: 3.162 ± 0.062	3.498LysThr: 3.498 ± 0.066	3.232LysVal: 3.232 ± 0.072	0.577LysTrp: 0.577 ± 0.025	2.037LysTyr: 2.037 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	8.601LeuAla: 8.601 ± 0.118	2.081LeuCys: 2.081 ± 0.053	5.479LeuAsp: 5.479 ± 0.075	5.523LeuGlu: 5.523 ± 0.086	3.919LeuPhe: 3.919 ± 0.087	6.252LeuGly: 6.252 ± 0.097	2.002LeuHis: 2.002 ± 0.048	5.239LeuIle: 5.239 ± 0.086	5.003LeuLys: 5.003 ± 0.076	10.225LeuLeu: 10.225 ± 0.169	2.652LeuMet: 2.652 ± 0.062	3.309LeuAsn: 3.309 ± 0.058	4.355LeuPro: 4.355 ± 0.063	2.959LeuGln: 2.959 ± 0.056	5.736LeuArg: 5.736 ± 0.1	7.373LeuSer: 7.373 ± 0.111	6.04LeuThr: 6.04 ± 0.091	5.586LeuVal: 5.586 ± 0.089	0.927LeuTrp: 0.927 ± 0.034	3.325LeuTyr: 3.325 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	2.604MetAla: 2.604 ± 0.051	0.357MetCys: 0.357 ± 0.018	1.635MetAsp: 1.635 ± 0.036	2.095MetGlu: 2.095 ± 0.049	0.86MetPhe: 0.86 ± 0.028	1.936MetGly: 1.936 ± 0.046	0.472MetHis: 0.472 ± 0.024	1.539MetIle: 1.539 ± 0.044	1.882MetLys: 1.882 ± 0.047	2.911MetLeu: 2.911 ± 0.072	0.781MetMet: 0.781 ± 0.033	1.233MetAsn: 1.233 ± 0.032	1.134MetPro: 1.134 ± 0.032	1.083MetGln: 1.083 ± 0.035	1.552MetArg: 1.552 ± 0.042	1.795MetSer: 1.795 ± 0.043	1.792MetThr: 1.792 ± 0.044	1.614MetVal: 1.614 ± 0.041	0.198MetTrp: 0.198 ± 0.017	0.655MetTyr: 0.655 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.516AsnAla: 3.516 ± 0.056	0.595AsnCys: 0.595 ± 0.027	2.037AsnAsp: 2.037 ± 0.048	2.317AsnGlu: 2.317 ± 0.056	1.348AsnPhe: 1.348 ± 0.039	3.258AsnGly: 3.258 ± 0.07	0.665AsnHis: 0.665 ± 0.028	2.369AsnIle: 2.369 ± 0.055	1.642AsnLys: 1.642 ± 0.045	3.11AsnLeu: 3.11 ± 0.062	1.049AsnMet: 1.049 ± 0.031	1.25AsnAsn: 1.25 ± 0.042	1.694AsnPro: 1.694 ± 0.04	1.051AsnGln: 1.051 ± 0.035	2.006AsnArg: 2.006 ± 0.047	1.791AsnSer: 1.791 ± 0.049	1.999AsnThr: 1.999 ± 0.05	2.381AsnVal: 2.381 ± 0.048	0.37AsnTrp: 0.37 ± 0.021	1.443AsnTyr: 1.443 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	3.389ProAla: 3.389 ± 0.076	0.594ProCys: 0.594 ± 0.029	2.633ProAsp: 2.633 ± 0.059	3.569ProGlu: 3.569 ± 0.067	1.553ProPhe: 1.553 ± 0.045	2.31ProGly: 2.31 ± 0.049	0.759ProHis: 0.759 ± 0.031	2.116ProIle: 2.116 ± 0.046	1.943ProLys: 1.943 ± 0.047	3.303ProLeu: 3.303 ± 0.052	1.004ProMet: 1.004 ± 0.037	1.332ProAsn: 1.332 ± 0.036	1.285ProPro: 1.285 ± 0.047	1.445ProGln: 1.445 ± 0.034	1.62ProArg: 1.62 ± 0.039	2.025ProSer: 2.025 ± 0.044	1.912ProThr: 1.912 ± 0.047	2.853ProVal: 2.853 ± 0.052	0.32ProTrp: 0.32 ± 0.02	1.522ProTyr: 1.522 ± 0.045	0.001ProXaa: 0.001 ± 0.001
Gln	2.798GlnAla: 2.798 ± 0.056	0.405GlnCys: 0.405 ± 0.021	1.573GlnAsp: 1.573 ± 0.045	2.432GlnGlu: 2.432 ± 0.051	1.127GlnPhe: 1.127 ± 0.035	1.953GlnGly: 1.953 ± 0.042	0.651GlnHis: 0.651 ± 0.028	2.129GlnIle: 2.129 ± 0.043	2.389GlnLys: 2.389 ± 0.055	3.105GlnLeu: 3.105 ± 0.053	1.161GlnMet: 1.161 ± 0.036	1.416GlnAsn: 1.416 ± 0.041	1.115GlnPro: 1.115 ± 0.037	1.486GlnGln: 1.486 ± 0.048	2.136GlnArg: 2.136 ± 0.057	1.964GlnSer: 1.964 ± 0.046	1.849GlnThr: 1.849 ± 0.047	1.879GlnVal: 1.879 ± 0.041	0.374GlnTrp: 0.374 ± 0.019	1.285GlnTyr: 1.285 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	4.88ArgAla: 4.88 ± 0.083	0.956ArgCys: 0.956 ± 0.038	2.923ArgAsp: 2.923 ± 0.057	4.676ArgGlu: 4.676 ± 0.086	2.264ArgPhe: 2.264 ± 0.043	3.497ArgGly: 3.497 ± 0.079	1.035ArgHis: 1.035 ± 0.035	3.455ArgIle: 3.455 ± 0.061	3.335ArgLys: 3.335 ± 0.065	4.938ArgLeu: 4.938 ± 0.085	1.76ArgMet: 1.76 ± 0.042	1.975ArgAsn: 1.975 ± 0.049	1.755ArgPro: 1.755 ± 0.047	2.149ArgGln: 2.149 ± 0.044	3.694ArgArg: 3.694 ± 0.078	3.05ArgSer: 3.05 ± 0.056	2.877ArgThr: 2.877 ± 0.055	3.375ArgVal: 3.375 ± 0.071	0.562ArgTrp: 0.562 ± 0.025	2.281ArgTyr: 2.281 ± 0.053	0.001ArgXaa: 0.001 ± 0.001
Ser	5.546SerAla: 5.546 ± 0.078	0.935SerCys: 0.935 ± 0.034	3.429SerAsp: 3.429 ± 0.065	3.844SerGlu: 3.844 ± 0.061	2.488SerPhe: 2.488 ± 0.055	5.316SerGly: 5.316 ± 0.098	1.075SerHis: 1.075 ± 0.04	3.519SerIle: 3.519 ± 0.067	2.718SerLys: 2.718 ± 0.054	5.53SerLeu: 5.53 ± 0.096	1.576SerMet: 1.576 ± 0.043	1.95SerAsn: 1.95 ± 0.049	2.13SerPro: 2.13 ± 0.054	1.75SerGln: 1.75 ± 0.048	3.172SerArg: 3.172 ± 0.056	3.379SerSer: 3.379 ± 0.085	3.018SerThr: 3.018 ± 0.066	4.498SerVal: 4.498 ± 0.071	0.579SerTrp: 0.579 ± 0.025	2.3SerTyr: 2.3 ± 0.054	0.001SerXaa: 0.001 ± 0.001
Thr	5.741ThrAla: 5.741 ± 0.097	0.759ThrCys: 0.759 ± 0.036	3.237ThrAsp: 3.237 ± 0.067	3.606ThrGlu: 3.606 ± 0.069	2.14ThrPhe: 2.14 ± 0.055	4.517ThrGly: 4.517 ± 0.084	1.072ThrHis: 1.072 ± 0.034	3.384ThrIle: 3.384 ± 0.069	2.36ThrLys: 2.36 ± 0.05	5.853ThrLeu: 5.853 ± 0.081	1.545ThrMet: 1.545 ± 0.039	1.732ThrAsn: 1.732 ± 0.041	2.683ThrPro: 2.683 ± 0.066	1.681ThrGln: 1.681 ± 0.049	2.473ThrArg: 2.473 ± 0.054	2.866ThrSer: 2.866 ± 0.059	2.99ThrThr: 2.99 ± 0.073	5.21ThrVal: 5.21 ± 0.087	0.505ThrTrp: 0.505 ± 0.024	1.973ThrTyr: 1.973 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	5.378ValAla: 5.378 ± 0.075	1.383ValCys: 1.383 ± 0.043	3.935ValAsp: 3.935 ± 0.063	4.433ValGlu: 4.433 ± 0.075	2.72ValPhe: 2.72 ± 0.061	4.493ValGly: 4.493 ± 0.08	1.249ValHis: 1.249 ± 0.038	4.199ValIle: 4.199 ± 0.078	3.538ValLys: 3.538 ± 0.067	7.049ValLeu: 7.049 ± 0.095	1.836ValMet: 1.836 ± 0.051	2.466ValAsn: 2.466 ± 0.061	2.84ValPro: 2.84 ± 0.058	2.044ValGln: 2.044 ± 0.043	3.798ValArg: 3.798 ± 0.069	4.712ValSer: 4.712 ± 0.072	4.488ValThr: 4.488 ± 0.104	4.55ValVal: 4.55 ± 0.087	0.696ValTrp: 0.696 ± 0.029	2.376ValTyr: 2.376 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.819TrpAla: 0.819 ± 0.029	0.195TrpCys: 0.195 ± 0.014	0.587TrpAsp: 0.587 ± 0.025	0.684TrpGlu: 0.684 ± 0.03	0.451TrpPhe: 0.451 ± 0.023	0.711TrpGly: 0.711 ± 0.023	0.201TrpHis: 0.201 ± 0.014	0.495TrpIle: 0.495 ± 0.023	0.532TrpLys: 0.532 ± 0.024	1.009TrpLeu: 1.009 ± 0.037	0.28TrpMet: 0.28 ± 0.016	0.46TrpAsn: 0.46 ± 0.026	0.22TrpPro: 0.22 ± 0.015	0.459TrpGln: 0.459 ± 0.02	0.597TrpArg: 0.597 ± 0.028	0.587TrpSer: 0.587 ± 0.025	0.54TrpThr: 0.54 ± 0.025	0.554TrpVal: 0.554 ± 0.024	0.129TrpTrp: 0.129 ± 0.014	0.423TrpTyr: 0.423 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.348TyrAla: 3.348 ± 0.059	0.754TyrCys: 0.754 ± 0.025	2.397TyrAsp: 2.397 ± 0.048	2.556TyrGlu: 2.556 ± 0.056	1.633TyrPhe: 1.633 ± 0.04	2.826TyrGly: 2.826 ± 0.048	0.808TyrHis: 0.808 ± 0.03	2.112TyrIle: 2.112 ± 0.047	1.504TyrLys: 1.504 ± 0.041	3.679TyrLeu: 3.679 ± 0.062	0.801TyrMet: 0.801 ± 0.029	1.4TyrAsn: 1.4 ± 0.04	1.395TyrPro: 1.395 ± 0.045	1.292TyrGln: 1.292 ± 0.036	2.296TyrArg: 2.296 ± 0.05	2.105TyrSer: 2.105 ± 0.057	2.187TyrThr: 2.187 ± 0.066	2.28TyrVal: 2.28 ± 0.056	0.379TyrTrp: 0.379 ± 0.02	1.655TyrTyr: 1.655 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.008XaaXaa: 0.008 ± 0.004

Statistics based on 3276 proteins (943759 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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