Amino acid dipepetide frequency for Mycobacterium phage Cornie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.352AlaAla: 12.352 ± 1.374
0.929AlaCys: 0.929 ± 0.206
6.777AlaAsp: 6.777 ± 0.522
6.996AlaGlu: 6.996 ± 0.856
2.46AlaPhe: 2.46 ± 0.32
8.636AlaGly: 8.636 ± 1.163
2.186AlaHis: 2.186 ± 0.384
4.7AlaIle: 4.7 ± 0.5
4.427AlaLys: 4.427 ± 0.475
7.269AlaLeu: 7.269 ± 0.585
3.006AlaMet: 3.006 ± 0.418
3.279AlaAsn: 3.279 ± 0.479
4.318AlaPro: 4.318 ± 0.496
5.302AlaGln: 5.302 ± 0.633
7.488AlaArg: 7.488 ± 0.811
5.356AlaSer: 5.356 ± 0.552
5.739AlaThr: 5.739 ± 0.515
6.723AlaVal: 6.723 ± 0.654
1.913AlaTrp: 1.913 ± 0.346
2.241AlaTyr: 2.241 ± 0.44
0.0AlaXaa: 0.0 ± 0.0
Cys
0.984CysAla: 0.984 ± 0.26
0.109CysCys: 0.109 ± 0.087
0.984CysAsp: 0.984 ± 0.255
0.82CysGlu: 0.82 ± 0.203
0.273CysPhe: 0.273 ± 0.133
1.202CysGly: 1.202 ± 0.291
0.601CysHis: 0.601 ± 0.153
0.383CysIle: 0.383 ± 0.156
0.547CysLys: 0.547 ± 0.175
0.492CysLeu: 0.492 ± 0.146
0.219CysMet: 0.219 ± 0.131
0.164CysAsn: 0.164 ± 0.088
1.202CysPro: 1.202 ± 0.267
0.273CysGln: 0.273 ± 0.133
0.929CysArg: 0.929 ± 0.242
0.875CysSer: 0.875 ± 0.307
0.656CysThr: 0.656 ± 0.163
0.656CysVal: 0.656 ± 0.183
0.219CysTrp: 0.219 ± 0.117
0.273CysTyr: 0.273 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
7.597AspAla: 7.597 ± 0.782
1.038AspCys: 1.038 ± 0.219
3.881AspAsp: 3.881 ± 0.601
4.482AspGlu: 4.482 ± 0.56
1.53AspPhe: 1.53 ± 0.301
6.012AspGly: 6.012 ± 0.608
1.64AspHis: 1.64 ± 0.25
2.35AspIle: 2.35 ± 0.291
1.804AspLys: 1.804 ± 0.361
5.739AspLeu: 5.739 ± 0.602
0.875AspMet: 0.875 ± 0.209
1.53AspAsn: 1.53 ± 0.276
3.826AspPro: 3.826 ± 0.458
2.35AspGln: 2.35 ± 0.344
3.717AspArg: 3.717 ± 0.603
3.006AspSer: 3.006 ± 0.436
3.935AspThr: 3.935 ± 0.42
4.373AspVal: 4.373 ± 0.469
1.64AspTrp: 1.64 ± 0.277
1.858AspTyr: 1.858 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
6.559GluAla: 6.559 ± 0.637
0.984GluCys: 0.984 ± 0.222
3.17GluAsp: 3.17 ± 0.368
3.061GluGlu: 3.061 ± 0.496
2.46GluPhe: 2.46 ± 0.393
4.537GluGly: 4.537 ± 0.591
1.202GluHis: 1.202 ± 0.294
2.897GluIle: 2.897 ± 0.433
2.296GluLys: 2.296 ± 0.364
5.575GluLeu: 5.575 ± 0.6
1.585GluMet: 1.585 ± 0.279
1.585GluAsn: 1.585 ± 0.291
3.334GluPro: 3.334 ± 0.393
2.569GluGln: 2.569 ± 0.322
4.482GluArg: 4.482 ± 0.826
3.115GluSer: 3.115 ± 0.397
3.225GluThr: 3.225 ± 0.461
4.7GluVal: 4.7 ± 0.42
1.476GluTrp: 1.476 ± 0.286
2.296GluTyr: 2.296 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
2.624PheAla: 2.624 ± 0.445
0.328PheCys: 0.328 ± 0.14
2.624PheAsp: 2.624 ± 0.454
2.132PheGlu: 2.132 ± 0.315
0.437PhePhe: 0.437 ± 0.15
2.951PheGly: 2.951 ± 0.479
0.656PheHis: 0.656 ± 0.255
1.421PheIle: 1.421 ± 0.267
0.711PheLys: 0.711 ± 0.183
1.968PheLeu: 1.968 ± 0.298
0.656PheMet: 0.656 ± 0.201
1.038PheAsn: 1.038 ± 0.255
1.421PhePro: 1.421 ± 0.314
0.656PheGln: 0.656 ± 0.205
1.749PheArg: 1.749 ± 0.305
1.913PheSer: 1.913 ± 0.411
2.787PheThr: 2.787 ± 0.382
2.296PheVal: 2.296 ± 0.373
0.547PheTrp: 0.547 ± 0.209
0.547PheTyr: 0.547 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
7.98GlyAla: 7.98 ± 1.058
1.148GlyCys: 1.148 ± 0.286
5.028GlyAsp: 5.028 ± 0.516
5.63GlyGlu: 5.63 ± 0.657
2.733GlyPhe: 2.733 ± 0.541
9.893GlyGly: 9.893 ± 1.855
2.022GlyHis: 2.022 ± 0.389
3.881GlyIle: 3.881 ± 0.739
3.662GlyLys: 3.662 ± 0.417
6.231GlyLeu: 6.231 ± 0.531
1.749GlyMet: 1.749 ± 0.491
3.498GlyAsn: 3.498 ± 0.477
4.81GlyPro: 4.81 ± 0.489
3.279GlyGln: 3.279 ± 0.552
4.974GlyArg: 4.974 ± 0.55
6.067GlySer: 6.067 ± 0.984
6.012GlyThr: 6.012 ± 0.78
6.34GlyVal: 6.34 ± 0.603
2.678GlyTrp: 2.678 ± 0.407
2.787GlyTyr: 2.787 ± 0.33
0.0GlyXaa: 0.0 ± 0.0
His
1.858HisAla: 1.858 ± 0.301
0.547HisCys: 0.547 ± 0.205
1.312HisAsp: 1.312 ± 0.336
1.312HisGlu: 1.312 ± 0.306
0.547HisPhe: 0.547 ± 0.167
1.694HisGly: 1.694 ± 0.351
0.875HisHis: 0.875 ± 0.242
1.366HisIle: 1.366 ± 0.272
0.929HisLys: 0.929 ± 0.22
2.35HisLeu: 2.35 ± 0.419
0.219HisMet: 0.219 ± 0.121
0.765HisAsn: 0.765 ± 0.209
1.312HisPro: 1.312 ± 0.278
0.711HisGln: 0.711 ± 0.179
1.804HisArg: 1.804 ± 0.339
1.366HisSer: 1.366 ± 0.256
1.148HisThr: 1.148 ± 0.283
2.022HisVal: 2.022 ± 0.408
0.492HisTrp: 0.492 ± 0.156
0.711HisTyr: 0.711 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
5.028IleAla: 5.028 ± 0.509
0.437IleCys: 0.437 ± 0.155
3.498IleAsp: 3.498 ± 0.429
3.389IleGlu: 3.389 ± 0.41
0.929IlePhe: 0.929 ± 0.224
4.318IleGly: 4.318 ± 0.468
1.257IleHis: 1.257 ± 0.223
1.749IleIle: 1.749 ± 0.32
1.202IleLys: 1.202 ± 0.28
2.787IleLeu: 2.787 ± 0.392
0.765IleMet: 0.765 ± 0.188
2.077IleAsn: 2.077 ± 0.385
3.389IlePro: 3.389 ± 0.45
1.202IleGln: 1.202 ± 0.233
2.46IleArg: 2.46 ± 0.365
2.241IleSer: 2.241 ± 0.332
3.826IleThr: 3.826 ± 0.74
3.225IleVal: 3.225 ± 0.472
0.984IleTrp: 0.984 ± 0.248
0.711IleTyr: 0.711 ± 0.176
0.0IleXaa: 0.0 ± 0.0
Lys
4.154LysAla: 4.154 ± 0.621
0.601LysCys: 0.601 ± 0.207
2.35LysAsp: 2.35 ± 0.35
1.968LysGlu: 1.968 ± 0.346
1.257LysPhe: 1.257 ± 0.299
2.35LysGly: 2.35 ± 0.38
1.093LysHis: 1.093 ± 0.296
1.257LysIle: 1.257 ± 0.281
1.476LysLys: 1.476 ± 0.325
2.842LysLeu: 2.842 ± 0.352
0.711LysMet: 0.711 ± 0.179
0.711LysAsn: 0.711 ± 0.151
2.842LysPro: 2.842 ± 0.372
1.53LysGln: 1.53 ± 0.297
2.405LysArg: 2.405 ± 0.338
1.858LysSer: 1.858 ± 0.286
1.913LysThr: 1.913 ± 0.256
2.296LysVal: 2.296 ± 0.392
0.711LysTrp: 0.711 ± 0.176
0.984LysTyr: 0.984 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
7.215LeuAla: 7.215 ± 0.716
0.437LeuCys: 0.437 ± 0.162
4.7LeuAsp: 4.7 ± 0.502
3.99LeuGlu: 3.99 ± 0.469
2.022LeuPhe: 2.022 ± 0.359
6.176LeuGly: 6.176 ± 0.693
1.421LeuHis: 1.421 ± 0.27
3.279LeuIle: 3.279 ± 0.551
2.569LeuLys: 2.569 ± 0.344
6.012LeuLeu: 6.012 ± 0.544
1.913LeuMet: 1.913 ± 0.364
2.569LeuAsn: 2.569 ± 0.341
4.864LeuPro: 4.864 ± 0.542
2.678LeuGln: 2.678 ± 0.362
5.192LeuArg: 5.192 ± 0.603
4.591LeuSer: 4.591 ± 0.509
5.192LeuThr: 5.192 ± 0.529
5.083LeuVal: 5.083 ± 0.509
1.585LeuTrp: 1.585 ± 0.304
2.186LeuTyr: 2.186 ± 0.298
0.0LeuXaa: 0.0 ± 0.0
Met
2.022MetAla: 2.022 ± 0.34
0.109MetCys: 0.109 ± 0.084
0.82MetAsp: 0.82 ± 0.201
1.257MetGlu: 1.257 ± 0.262
1.038MetPhe: 1.038 ± 0.259
2.022MetGly: 2.022 ± 0.429
0.273MetHis: 0.273 ± 0.106
1.202MetIle: 1.202 ± 0.29
0.547MetLys: 0.547 ± 0.147
1.64MetLeu: 1.64 ± 0.294
0.273MetMet: 0.273 ± 0.092
1.202MetAsn: 1.202 ± 0.224
1.585MetPro: 1.585 ± 0.249
0.82MetGln: 0.82 ± 0.218
1.585MetArg: 1.585 ± 0.238
2.514MetSer: 2.514 ± 0.369
2.296MetThr: 2.296 ± 0.356
1.202MetVal: 1.202 ± 0.288
0.055MetTrp: 0.055 ± 0.055
0.328MetTyr: 0.328 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.826AsnAla: 3.826 ± 0.463
0.219AsnCys: 0.219 ± 0.119
1.968AsnAsp: 1.968 ± 0.336
1.694AsnGlu: 1.694 ± 0.299
0.82AsnPhe: 0.82 ± 0.281
4.099AsnGly: 4.099 ± 0.446
0.656AsnHis: 0.656 ± 0.169
1.366AsnIle: 1.366 ± 0.277
0.601AsnLys: 0.601 ± 0.172
2.842AsnLeu: 2.842 ± 0.394
0.547AsnMet: 0.547 ± 0.146
1.421AsnAsn: 1.421 ± 0.263
2.569AsnPro: 2.569 ± 0.38
1.366AsnGln: 1.366 ± 0.382
2.569AsnArg: 2.569 ± 0.361
1.148AsnSer: 1.148 ± 0.273
2.787AsnThr: 2.787 ± 0.313
2.296AsnVal: 2.296 ± 0.33
0.328AsnTrp: 0.328 ± 0.11
0.765AsnTyr: 0.765 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
5.138ProAla: 5.138 ± 0.655
0.82ProCys: 0.82 ± 0.211
4.209ProAsp: 4.209 ± 0.472
4.864ProGlu: 4.864 ± 0.558
1.968ProPhe: 1.968 ± 0.346
6.613ProGly: 6.613 ± 0.669
1.53ProHis: 1.53 ± 0.336
2.405ProIle: 2.405 ± 0.392
2.46ProLys: 2.46 ± 0.314
3.771ProLeu: 3.771 ± 0.492
1.421ProMet: 1.421 ± 0.267
1.968ProAsn: 1.968 ± 0.358
3.935ProPro: 3.935 ± 0.52
2.405ProGln: 2.405 ± 0.322
3.607ProArg: 3.607 ± 0.583
3.553ProSer: 3.553 ± 0.35
3.553ProThr: 3.553 ± 0.633
3.498ProVal: 3.498 ± 0.439
1.257ProTrp: 1.257 ± 0.284
1.749ProTyr: 1.749 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
4.263GlnAla: 4.263 ± 0.422
0.383GlnCys: 0.383 ± 0.18
1.53GlnAsp: 1.53 ± 0.328
2.022GlnGlu: 2.022 ± 0.381
1.038GlnPhe: 1.038 ± 0.234
2.624GlnGly: 2.624 ± 0.355
0.984GlnHis: 0.984 ± 0.177
2.514GlnIle: 2.514 ± 0.397
1.64GlnLys: 1.64 ± 0.275
2.951GlnLeu: 2.951 ± 0.412
1.257GlnMet: 1.257 ± 0.372
1.148GlnAsn: 1.148 ± 0.326
3.17GlnPro: 3.17 ± 0.452
1.694GlnGln: 1.694 ± 0.367
2.733GlnArg: 2.733 ± 0.455
2.514GlnSer: 2.514 ± 0.403
2.241GlnThr: 2.241 ± 0.297
2.405GlnVal: 2.405 ± 0.34
0.492GlnTrp: 0.492 ± 0.185
0.601GlnTyr: 0.601 ± 0.182
0.0GlnXaa: 0.0 ± 0.0
Arg
6.012ArgAla: 6.012 ± 0.511
1.202ArgCys: 1.202 ± 0.28
3.443ArgAsp: 3.443 ± 0.516
4.373ArgGlu: 4.373 ± 0.586
2.405ArgPhe: 2.405 ± 0.411
4.7ArgGly: 4.7 ± 0.41
1.749ArgHis: 1.749 ± 0.389
3.717ArgIle: 3.717 ± 0.611
2.296ArgLys: 2.296 ± 0.476
4.974ArgLeu: 4.974 ± 0.567
2.46ArgMet: 2.46 ± 0.343
2.186ArgAsn: 2.186 ± 0.307
2.951ArgPro: 2.951 ± 0.499
2.46ArgGln: 2.46 ± 0.419
6.122ArgArg: 6.122 ± 0.816
4.427ArgSer: 4.427 ± 0.454
3.771ArgThr: 3.771 ± 0.485
5.411ArgVal: 5.411 ± 0.677
1.913ArgTrp: 1.913 ± 0.339
1.53ArgTyr: 1.53 ± 0.241
0.0ArgXaa: 0.0 ± 0.0
Ser
5.903SerAla: 5.903 ± 0.834
0.164SerCys: 0.164 ± 0.109
4.373SerAsp: 4.373 ± 0.381
3.443SerGlu: 3.443 ± 0.362
1.804SerPhe: 1.804 ± 0.269
5.958SerGly: 5.958 ± 0.788
0.929SerHis: 0.929 ± 0.233
2.186SerIle: 2.186 ± 0.256
2.186SerLys: 2.186 ± 0.42
3.826SerLeu: 3.826 ± 0.41
1.749SerMet: 1.749 ± 0.266
2.405SerAsn: 2.405 ± 0.343
3.389SerPro: 3.389 ± 0.469
1.968SerGln: 1.968 ± 0.326
3.279SerArg: 3.279 ± 0.529
3.115SerSer: 3.115 ± 0.534
3.17SerThr: 3.17 ± 0.373
4.099SerVal: 4.099 ± 0.548
1.53SerTrp: 1.53 ± 0.287
1.312SerTyr: 1.312 ± 0.245
0.0SerXaa: 0.0 ± 0.0
Thr
6.723ThrAla: 6.723 ± 0.619
0.601ThrCys: 0.601 ± 0.175
4.045ThrAsp: 4.045 ± 0.461
3.443ThrGlu: 3.443 ± 0.414
1.968ThrPhe: 1.968 ± 0.389
6.777ThrGly: 6.777 ± 0.715
1.257ThrHis: 1.257 ± 0.217
3.115ThrIle: 3.115 ± 0.692
1.64ThrLys: 1.64 ± 0.307
4.373ThrLeu: 4.373 ± 0.558
1.038ThrMet: 1.038 ± 0.188
2.624ThrAsn: 2.624 ± 0.44
5.575ThrPro: 5.575 ± 0.644
2.241ThrGln: 2.241 ± 0.513
4.045ThrArg: 4.045 ± 0.492
3.17ThrSer: 3.17 ± 0.415
4.755ThrThr: 4.755 ± 0.646
4.919ThrVal: 4.919 ± 0.705
1.366ThrTrp: 1.366 ± 0.275
1.749ThrTyr: 1.749 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
7.379ValAla: 7.379 ± 0.7
1.202ValCys: 1.202 ± 0.285
4.755ValAsp: 4.755 ± 0.483
4.154ValGlu: 4.154 ± 0.472
2.296ValPhe: 2.296 ± 0.283
5.52ValGly: 5.52 ± 0.574
1.968ValHis: 1.968 ± 0.367
3.717ValIle: 3.717 ± 0.386
2.897ValLys: 2.897 ± 0.375
4.482ValLeu: 4.482 ± 0.606
1.476ValMet: 1.476 ± 0.277
2.132ValAsn: 2.132 ± 0.343
4.099ValPro: 4.099 ± 0.488
2.405ValGln: 2.405 ± 0.383
4.974ValArg: 4.974 ± 0.675
3.498ValSer: 3.498 ± 0.496
4.646ValThr: 4.646 ± 0.788
5.575ValVal: 5.575 ± 0.69
1.476ValTrp: 1.476 ± 0.292
1.804ValTyr: 1.804 ± 0.339
0.0ValXaa: 0.0 ± 0.0
Trp
2.022TrpAla: 2.022 ± 0.293
0.328TrpCys: 0.328 ± 0.141
2.022TrpAsp: 2.022 ± 0.349
0.929TrpGlu: 0.929 ± 0.233
0.656TrpPhe: 0.656 ± 0.146
1.858TrpGly: 1.858 ± 0.366
0.383TrpHis: 0.383 ± 0.157
0.984TrpIle: 0.984 ± 0.235
0.711TrpLys: 0.711 ± 0.218
1.476TrpLeu: 1.476 ± 0.256
0.656TrpMet: 0.656 ± 0.181
0.437TrpAsn: 0.437 ± 0.165
1.148TrpPro: 1.148 ± 0.223
0.984TrpGln: 0.984 ± 0.242
1.749TrpArg: 1.749 ± 0.331
1.202TrpSer: 1.202 ± 0.271
1.476TrpThr: 1.476 ± 0.355
1.366TrpVal: 1.366 ± 0.282
0.82TrpTrp: 0.82 ± 0.227
0.601TrpTyr: 0.601 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.514TyrAla: 2.514 ± 0.365
0.273TyrCys: 0.273 ± 0.117
1.694TyrAsp: 1.694 ± 0.279
1.148TyrGlu: 1.148 ± 0.284
0.82TyrPhe: 0.82 ± 0.238
2.46TyrGly: 2.46 ± 0.553
0.656TyrHis: 0.656 ± 0.171
0.984TyrIle: 0.984 ± 0.215
0.711TyrLys: 0.711 ± 0.176
2.077TyrLeu: 2.077 ± 0.382
0.164TyrMet: 0.164 ± 0.11
1.148TyrAsn: 1.148 ± 0.233
0.984TyrPro: 0.984 ± 0.23
1.312TyrGln: 1.312 ± 0.23
2.241TyrArg: 2.241 ± 0.304
1.257TyrSer: 1.257 ± 0.26
2.296TyrThr: 2.296 ± 0.387
1.968TyrVal: 1.968 ± 0.334
0.328TyrTrp: 0.328 ± 0.14
0.875TyrTyr: 0.875 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (18297 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski