Methylobacterium frigidaeris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G8MQU0|A0A2G8MQU0_9RHIZ Adenylosuccinate synthase OS=Methylobacterium frigidaeris OX=2038277 GN=CS379_00435 PE=4 SV=1
MM1 pKa = 7.47LSAALRR7 pKa = 11.84ALGCLTADD15 pKa = 3.55RR16 pKa = 11.84VLPTADD22 pKa = 3.98LGTVTVDD29 pKa = 3.78TVPDD33 pKa = 3.66GCGPGWPCEE42 pKa = 4.15ALPGTPPSVADD53 pKa = 4.02RR54 pKa = 11.84QAAPSADD61 pKa = 3.43DD62 pKa = 5.37LYY64 pKa = 10.52PQIAGVLTPRR74 pKa = 11.84GPAWGTDD81 pKa = 3.18EE82 pKa = 5.98AGDD85 pKa = 3.84GRR87 pKa = 11.84GAGPVMRR94 pKa = 11.84GVWRR98 pKa = 11.84ALSQWVARR106 pKa = 11.84QNADD110 pKa = 2.54EE111 pKa = 4.31WTLATQALPSAITYY125 pKa = 8.4SLPDD129 pKa = 3.22WEE131 pKa = 5.2AEE133 pKa = 4.04YY134 pKa = 11.06GLPDD138 pKa = 3.9PCSPGGFTTEE148 pKa = 3.51SRR150 pKa = 11.84IAAVRR155 pKa = 11.84ARR157 pKa = 11.84FGARR161 pKa = 11.84GGSSPAYY168 pKa = 8.78FICLAASLGYY178 pKa = 10.38AITIEE183 pKa = 4.43EE184 pKa = 4.42PTQFLCDD191 pKa = 3.47VSAVGTDD198 pKa = 3.3ALVEE202 pKa = 4.15TWFTCDD208 pKa = 4.53GGEE211 pKa = 4.33CDD213 pKa = 4.41GDD215 pKa = 4.34PIEE218 pKa = 4.86GFAMAPAADD227 pKa = 4.49DD228 pKa = 3.66TDD230 pKa = 3.94QVGGDD235 pKa = 3.65PFVEE239 pKa = 5.16GWCVADD245 pKa = 5.3EE246 pKa = 4.66GMCDD250 pKa = 3.4VTPLEE255 pKa = 5.04FYY257 pKa = 9.37ATNPNADD264 pKa = 2.95AWQFWVVHH272 pKa = 5.64VAAQGDD278 pKa = 3.76TWFACDD284 pKa = 4.76EE285 pKa = 4.71GEE287 pKa = 5.06CDD289 pKa = 3.48TDD291 pKa = 4.4PIEE294 pKa = 4.65GFRR297 pKa = 11.84SAADD301 pKa = 3.73LEE303 pKa = 4.47CLLRR307 pKa = 11.84RR308 pKa = 11.84VSPAHH313 pKa = 5.38TALVIAYY320 pKa = 9.24DD321 pKa = 3.75GG322 pKa = 3.79

Molecular weight: 33.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G8MDM3|A0A2G8MDM3_9RHIZ Potassium transporter OS=Methylobacterium frigidaeris OX=2038277 GN=CS379_23910 PE=4 SV=1
MM1 pKa = 7.66MIRR4 pKa = 11.84SNALRR9 pKa = 11.84AGLVLLALLAGLLTASPMVVTGIIAALGLAGAVALAGTRR48 pKa = 11.84AQQAPVLVPVRR59 pKa = 11.84ARR61 pKa = 11.84NRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 3.51

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6432

0

6432

1677552

20

2481

260.8

27.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.507 ± 0.052

0.81 ± 0.01

5.412 ± 0.027

5.524 ± 0.027

3.283 ± 0.02

9.239 ± 0.033

1.969 ± 0.014

4.204 ± 0.021

2.304 ± 0.03

10.671 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.015

1.946 ± 0.016

5.988 ± 0.029

2.746 ± 0.022

8.319 ± 0.031

4.695 ± 0.022

5.285 ± 0.022

7.773 ± 0.025

1.259 ± 0.012

1.993 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski