Nocardia phage NTR1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A660GBM8|A0A660GBM8_9CAUD Uncharacterized protein OS=Nocardia phage NTR1 OX=2026083 GN=NTR1_19 PE=4 SV=1
MM1 pKa = 8.03ADD3 pKa = 3.74PFASARR9 pKa = 11.84DD10 pKa = 3.65VGDD13 pKa = 3.93LFEE16 pKa = 5.92ASYY19 pKa = 11.48DD20 pKa = 3.63SDD22 pKa = 4.75LDD24 pKa = 3.92CGHH27 pKa = 6.29YY28 pKa = 10.44VHH30 pKa = 7.12AGDD33 pKa = 5.54LIAYY37 pKa = 9.6VEE39 pKa = 4.68GEE41 pKa = 4.32LSCEE45 pKa = 4.17EE46 pKa = 4.21CRR48 pKa = 11.84EE49 pKa = 3.92EE50 pKa = 5.13ALAA53 pKa = 5.23

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A660GBP2|A0A660GBP2_9CAUD Putative major tail protein OS=Nocardia phage NTR1 OX=2026083 GN=NTR1_40 PE=4 SV=1
MM1 pKa = 7.63ARR3 pKa = 11.84RR4 pKa = 11.84AYY6 pKa = 9.85DD7 pKa = 3.24RR8 pKa = 11.84KK9 pKa = 10.57RR10 pKa = 11.84PDD12 pKa = 2.88VAFAIEE18 pKa = 4.46MGWEE22 pKa = 4.07FCGFAGSGHH31 pKa = 5.9LQFRR35 pKa = 11.84HH36 pKa = 5.79PKK38 pKa = 8.32VAVRR42 pKa = 11.84LIMPATPSEE51 pKa = 4.03GRR53 pKa = 11.84GTKK56 pKa = 10.46NSIAWVKK63 pKa = 10.6RR64 pKa = 11.84NTPRR68 pKa = 11.84EE69 pKa = 4.08EE70 pKa = 3.83

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

19771

42

1861

203.8

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.018 ± 0.479

1.138 ± 0.163

6.348 ± 0.212

6.58 ± 0.268

2.848 ± 0.134

8.376 ± 0.185

1.937 ± 0.194

4.588 ± 0.228

2.61 ± 0.164

8.229 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.131

2.989 ± 0.194

5.766 ± 0.264

3.343 ± 0.189

6.499 ± 0.316

5.093 ± 0.182

7.061 ± 0.264

7.789 ± 0.182

2.109 ± 0.168

2.478 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski