Amino acid dipepetide frequency for Bacteroidales bacterium WCE2008

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.853AlaAla: 7.853 ± 0.147	1.0AlaCys: 1.0 ± 0.041	5.028AlaAsp: 5.028 ± 0.094	5.485AlaGlu: 5.485 ± 0.108	3.408AlaPhe: 3.408 ± 0.069	6.69AlaGly: 6.69 ± 0.114	1.207AlaHis: 1.207 ± 0.042	5.197AlaIle: 5.197 ± 0.112	4.39AlaLys: 4.39 ± 0.099	7.215AlaLeu: 7.215 ± 0.106	2.113AlaMet: 2.113 ± 0.062	2.847AlaAsn: 2.847 ± 0.073	2.783AlaPro: 2.783 ± 0.065	2.243AlaGln: 2.243 ± 0.059	3.717AlaArg: 3.717 ± 0.083	5.257AlaSer: 5.257 ± 0.095	3.883AlaThr: 3.883 ± 0.083	6.092AlaVal: 6.092 ± 0.094	0.842AlaTrp: 0.842 ± 0.035	2.803AlaTyr: 2.803 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.857CysAla: 0.857 ± 0.037	0.205CysCys: 0.205 ± 0.018	0.787CysAsp: 0.787 ± 0.032	0.675CysGlu: 0.675 ± 0.032	0.565CysPhe: 0.565 ± 0.023	1.148CysGly: 1.148 ± 0.039	0.251CysHis: 0.251 ± 0.02	0.809CysIle: 0.809 ± 0.032	0.572CysLys: 0.572 ± 0.028	0.946CysLeu: 0.946 ± 0.038	0.258CysMet: 0.258 ± 0.018	0.529CysAsn: 0.529 ± 0.027	0.514CysPro: 0.514 ± 0.029	0.275CysGln: 0.275 ± 0.018	0.677CysArg: 0.677 ± 0.029	0.878CysSer: 0.878 ± 0.036	0.614CysThr: 0.614 ± 0.029	0.754CysVal: 0.754 ± 0.036	0.124CysTrp: 0.124 ± 0.013	0.502CysTyr: 0.502 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.807AspAla: 4.807 ± 0.081	0.699AspCys: 0.699 ± 0.034	3.284AspAsp: 3.284 ± 0.084	3.947AspGlu: 3.947 ± 0.077	3.344AspPhe: 3.344 ± 0.067	5.499AspGly: 5.499 ± 0.108	1.032AspHis: 1.032 ± 0.039	4.556AspIle: 4.556 ± 0.08	3.964AspLys: 3.964 ± 0.085	4.96AspLeu: 4.96 ± 0.08	1.739AspMet: 1.739 ± 0.051	2.83AspAsn: 2.83 ± 0.062	2.614AspPro: 2.614 ± 0.059	1.28AspGln: 1.28 ± 0.044	3.072AspArg: 3.072 ± 0.061	3.879AspSer: 3.879 ± 0.097	2.633AspThr: 2.633 ± 0.065	4.054AspVal: 4.054 ± 0.074	0.934AspTrp: 0.934 ± 0.035	3.208AspTyr: 3.208 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	5.387GluAla: 5.387 ± 0.108	0.654GluCys: 0.654 ± 0.03	3.949GluAsp: 3.949 ± 0.075	4.531GluGlu: 4.531 ± 0.102	2.614GluPhe: 2.614 ± 0.058	4.599GluGly: 4.599 ± 0.089	1.01GluHis: 1.01 ± 0.038	4.519GluIle: 4.519 ± 0.087	4.858GluLys: 4.858 ± 0.096	5.207GluLeu: 5.207 ± 0.1	1.823GluMet: 1.823 ± 0.054	3.177GluAsn: 3.177 ± 0.068	2.061GluPro: 2.061 ± 0.058	1.758GluGln: 1.758 ± 0.058	2.977GluArg: 2.977 ± 0.074	3.548GluSer: 3.548 ± 0.064	3.387GluThr: 3.387 ± 0.068	4.124GluVal: 4.124 ± 0.084	0.723GluTrp: 0.723 ± 0.031	2.851GluTyr: 2.851 ± 0.072	0.0GluXaa: 0.0 ± 0.0
Phe	3.408PheAla: 3.408 ± 0.075	0.638PheCys: 0.638 ± 0.032	3.025PheAsp: 3.025 ± 0.061	2.409PheGlu: 2.409 ± 0.058	2.04PhePhe: 2.04 ± 0.06	3.761PheGly: 3.761 ± 0.077	0.765PheHis: 0.765 ± 0.035	2.89PheIle: 2.89 ± 0.077	2.234PheLys: 2.234 ± 0.065	3.711PheLeu: 3.711 ± 0.071	1.298PheMet: 1.298 ± 0.039	2.088PheAsn: 2.088 ± 0.052	1.794PhePro: 1.794 ± 0.05	0.966PheGln: 0.966 ± 0.036	2.477PheArg: 2.477 ± 0.068	3.603PheSer: 3.603 ± 0.085	2.552PheThr: 2.552 ± 0.063	3.004PheVal: 3.004 ± 0.066	0.583PheTrp: 0.583 ± 0.029	1.724PheTyr: 1.724 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	5.422GlyAla: 5.422 ± 0.098	0.988GlyCys: 0.988 ± 0.038	4.357GlyAsp: 4.357 ± 0.074	4.696GlyGlu: 4.696 ± 0.087	3.518GlyPhe: 3.518 ± 0.074	5.528GlyGly: 5.528 ± 0.113	1.454GlyHis: 1.454 ± 0.051	5.452GlyIle: 5.452 ± 0.101	5.508GlyLys: 5.508 ± 0.097	6.604GlyLeu: 6.604 ± 0.1	2.229GlyMet: 2.229 ± 0.061	3.501GlyAsn: 3.501 ± 0.091	1.879GlyPro: 1.879 ± 0.05	2.076GlyGln: 2.076 ± 0.058	3.937GlyArg: 3.937 ± 0.081	4.827GlySer: 4.827 ± 0.099	4.499GlyThr: 4.499 ± 0.087	5.433GlyVal: 5.433 ± 0.094	1.12GlyTrp: 1.12 ± 0.047	3.563GlyTyr: 3.563 ± 0.077	0.0GlyXaa: 0.0 ± 0.0
His	1.035HisAla: 1.035 ± 0.04	0.229HisCys: 0.229 ± 0.019	0.959HisAsp: 0.959 ± 0.037	0.983HisGlu: 0.983 ± 0.04	0.882HisPhe: 0.882 ± 0.036	1.387HisGly: 1.387 ± 0.037	0.38HisHis: 0.38 ± 0.031	1.202HisIle: 1.202 ± 0.04	0.9HisLys: 0.9 ± 0.038	1.453HisLeu: 1.453 ± 0.044	0.384HisMet: 0.384 ± 0.019	0.838HisAsn: 0.838 ± 0.031	0.903HisPro: 0.903 ± 0.039	0.406HisGln: 0.406 ± 0.025	0.839HisArg: 0.839 ± 0.037	1.043HisSer: 1.043 ± 0.039	0.849HisThr: 0.849 ± 0.034	1.121HisVal: 1.121 ± 0.043	0.194HisTrp: 0.194 ± 0.018	0.804HisTyr: 0.804 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.665IleAla: 5.665 ± 0.095	0.93IleCys: 0.93 ± 0.037	4.467IleAsp: 4.467 ± 0.083	4.055IleGlu: 4.055 ± 0.082	2.713IlePhe: 2.713 ± 0.071	4.824IleGly: 4.824 ± 0.093	1.116IleHis: 1.116 ± 0.042	4.308IleIle: 4.308 ± 0.098	3.452IleLys: 3.452 ± 0.067	5.81IleLeu: 5.81 ± 0.112	1.604IleMet: 1.604 ± 0.051	2.79IleAsn: 2.79 ± 0.065	3.207IlePro: 3.207 ± 0.079	1.616IleGln: 1.616 ± 0.045	3.466IleArg: 3.466 ± 0.073	5.099IleSer: 5.099 ± 0.088	3.522IleThr: 3.522 ± 0.084	4.63IleVal: 4.63 ± 0.082	0.737IleTrp: 0.737 ± 0.033	2.463IleTyr: 2.463 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	5.411LysAla: 5.411 ± 0.116	0.486LysCys: 0.486 ± 0.029	4.275LysAsp: 4.275 ± 0.09	4.463LysGlu: 4.463 ± 0.101	2.225LysPhe: 2.225 ± 0.052	4.31LysGly: 4.31 ± 0.082	0.867LysHis: 0.867 ± 0.036	4.135LysIle: 4.135 ± 0.086	4.223LysLys: 4.223 ± 0.084	4.576LysLeu: 4.576 ± 0.068	1.984LysMet: 1.984 ± 0.055	2.718LysAsn: 2.718 ± 0.075	2.094LysPro: 2.094 ± 0.058	1.372LysGln: 1.372 ± 0.046	2.484LysArg: 2.484 ± 0.061	3.315LysSer: 3.315 ± 0.072	3.472LysThr: 3.472 ± 0.076	4.618LysVal: 4.618 ± 0.096	0.797LysTrp: 0.797 ± 0.033	2.79LysTyr: 2.79 ± 0.072	0.0LysXaa: 0.0 ± 0.0
Leu	6.848LeuAla: 6.848 ± 0.103	1.198LeuCys: 1.198 ± 0.043	5.261LeuAsp: 5.261 ± 0.091	5.255LeuGlu: 5.255 ± 0.104	3.819LeuPhe: 3.819 ± 0.079	6.297LeuGly: 6.297 ± 0.113	1.423LeuHis: 1.423 ± 0.046	5.002LeuIle: 5.002 ± 0.094	5.537LeuLys: 5.537 ± 0.103	7.503LeuLeu: 7.503 ± 0.133	2.343LeuMet: 2.343 ± 0.065	3.567LeuAsn: 3.567 ± 0.08	3.767LeuPro: 3.767 ± 0.073	2.458LeuGln: 2.458 ± 0.059	4.517LeuArg: 4.517 ± 0.084	6.759LeuSer: 6.759 ± 0.106	4.785LeuThr: 4.785 ± 0.084	5.429LeuVal: 5.429 ± 0.084	1.014LeuTrp: 1.014 ± 0.041	3.316LeuTyr: 3.316 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	2.337MetAla: 2.337 ± 0.063	0.182MetCys: 0.182 ± 0.017	1.825MetAsp: 1.825 ± 0.051	1.725MetGlu: 1.725 ± 0.052	1.055MetPhe: 1.055 ± 0.04	1.765MetGly: 1.765 ± 0.051	0.436MetHis: 0.436 ± 0.025	1.793MetIle: 1.793 ± 0.052	2.187MetLys: 2.187 ± 0.058	2.307MetLeu: 2.307 ± 0.059	0.788MetMet: 0.788 ± 0.038	1.28MetAsn: 1.28 ± 0.04	1.164MetPro: 1.164 ± 0.039	0.766MetGln: 0.766 ± 0.033	1.315MetArg: 1.315 ± 0.046	1.711MetSer: 1.711 ± 0.052	1.607MetThr: 1.607 ± 0.049	1.779MetVal: 1.779 ± 0.051	0.258MetTrp: 0.258 ± 0.02	0.809MetTyr: 0.809 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.304AsnAla: 3.304 ± 0.07	0.522AsnCys: 0.522 ± 0.029	2.314AsnAsp: 2.314 ± 0.059	2.385AsnGlu: 2.385 ± 0.057	1.893AsnPhe: 1.893 ± 0.056	3.703AsnGly: 3.703 ± 0.081	0.715AsnHis: 0.715 ± 0.029	3.12AsnIle: 3.12 ± 0.062	2.183AsnLys: 2.183 ± 0.06	3.893AsnLeu: 3.893 ± 0.078	1.144AsnMet: 1.144 ± 0.043	1.948AsnAsn: 1.948 ± 0.063	2.434AsnPro: 2.434 ± 0.062	1.079AsnGln: 1.079 ± 0.041	1.956AsnArg: 1.956 ± 0.064	2.789AsnSer: 2.789 ± 0.065	2.258AsnThr: 2.258 ± 0.067	3.17AsnVal: 3.17 ± 0.078	0.617AsnTrp: 0.617 ± 0.032	2.082AsnTyr: 2.082 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	3.452ProAla: 3.452 ± 0.076	0.42ProCys: 0.42 ± 0.025	3.133ProAsp: 3.133 ± 0.065	3.877ProGlu: 3.877 ± 0.075	1.763ProPhe: 1.763 ± 0.044	3.049ProGly: 3.049 ± 0.077	0.618ProHis: 0.618 ± 0.033	1.989ProIle: 1.989 ± 0.05	1.982ProLys: 1.982 ± 0.058	3.058ProLeu: 3.058 ± 0.07	0.971ProMet: 0.971 ± 0.036	1.263ProAsn: 1.263 ± 0.047	0.795ProPro: 0.795 ± 0.034	1.156ProGln: 1.156 ± 0.038	1.369ProArg: 1.369 ± 0.047	2.678ProSer: 2.678 ± 0.067	1.634ProThr: 1.634 ± 0.051	3.535ProVal: 3.535 ± 0.072	0.468ProTrp: 0.468 ± 0.025	1.763ProTyr: 1.763 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	2.029GlnAla: 2.029 ± 0.056	0.24GlnCys: 0.24 ± 0.019	1.43GlnAsp: 1.43 ± 0.046	1.655GlnGlu: 1.655 ± 0.053	1.145GlnPhe: 1.145 ± 0.039	1.643GlnGly: 1.643 ± 0.049	0.422GlnHis: 0.422 ± 0.024	1.854GlnIle: 1.854 ± 0.049	1.821GlnLys: 1.821 ± 0.052	2.281GlnLeu: 2.281 ± 0.057	0.854GlnMet: 0.854 ± 0.033	1.23GlnAsn: 1.23 ± 0.045	0.983GlnPro: 0.983 ± 0.034	0.839GlnGln: 0.839 ± 0.04	1.306GlnArg: 1.306 ± 0.044	1.542GlnSer: 1.542 ± 0.046	1.539GlnThr: 1.539 ± 0.051	1.57GlnVal: 1.57 ± 0.048	0.352GlnTrp: 0.352 ± 0.024	1.172GlnTyr: 1.172 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	3.107ArgAla: 3.107 ± 0.071	0.526ArgCys: 0.526 ± 0.027	2.565ArgAsp: 2.565 ± 0.056	3.096ArgGlu: 3.096 ± 0.068	2.55ArgPhe: 2.55 ± 0.053	2.94ArgGly: 2.94 ± 0.068	1.048ArgHis: 1.048 ± 0.039	3.706ArgIle: 3.706 ± 0.08	3.553ArgLys: 3.553 ± 0.087	4.631ArgLeu: 4.631 ± 0.083	1.557ArgMet: 1.557 ± 0.05	2.493ArgAsn: 2.493 ± 0.062	1.788ArgPro: 1.788 ± 0.053	1.597ArgGln: 1.597 ± 0.059	2.705ArgArg: 2.705 ± 0.061	2.882ArgSer: 2.882 ± 0.068	2.534ArgThr: 2.534 ± 0.058	2.766ArgVal: 2.766 ± 0.071	0.612ArgTrp: 0.612 ± 0.032	2.469ArgTyr: 2.469 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	5.499SerAla: 5.499 ± 0.096	0.911SerCys: 0.911 ± 0.033	4.166SerAsp: 4.166 ± 0.085	3.999SerGlu: 3.999 ± 0.062	3.397SerPhe: 3.397 ± 0.078	5.935SerGly: 5.935 ± 0.116	1.079SerHis: 1.079 ± 0.039	4.366SerIle: 4.366 ± 0.075	3.361SerLys: 3.361 ± 0.077	6.152SerLeu: 6.152 ± 0.112	1.674SerMet: 1.674 ± 0.049	2.517SerAsn: 2.517 ± 0.069	2.412SerPro: 2.412 ± 0.052	1.728SerGln: 1.728 ± 0.052	3.437SerArg: 3.437 ± 0.074	4.921SerSer: 4.921 ± 0.136	3.228SerThr: 3.228 ± 0.067	4.869SerVal: 4.869 ± 0.091	0.927SerTrp: 0.927 ± 0.044	3.072SerTyr: 3.072 ± 0.081	0.0SerXaa: 0.0 ± 0.0
Thr	4.135ThrAla: 4.135 ± 0.073	0.496ThrCys: 0.496 ± 0.026	3.378ThrAsp: 3.378 ± 0.075	2.944ThrGlu: 2.944 ± 0.068	2.476ThrPhe: 2.476 ± 0.051	4.793ThrGly: 4.793 ± 0.096	0.838ThrHis: 0.838 ± 0.036	3.655ThrIle: 3.655 ± 0.078	2.478ThrLys: 2.478 ± 0.058	4.928ThrLeu: 4.928 ± 0.084	1.178ThrMet: 1.178 ± 0.044	1.897ThrAsn: 1.897 ± 0.056	2.59ThrPro: 2.59 ± 0.06	1.183ThrGln: 1.183 ± 0.037	2.33ThrArg: 2.33 ± 0.055	3.387ThrSer: 3.387 ± 0.069	2.814ThrThr: 2.814 ± 0.064	4.488ThrVal: 4.488 ± 0.086	0.695ThrTrp: 0.695 ± 0.033	2.28ThrTyr: 2.28 ± 0.067	0.0ThrXaa: 0.0 ± 0.0
Val	5.586ValAla: 5.586 ± 0.101	0.905ValCys: 0.905 ± 0.038	4.27ValAsp: 4.27 ± 0.085	4.38ValGlu: 4.38 ± 0.092	2.961ValPhe: 2.961 ± 0.064	4.491ValGly: 4.491 ± 0.091	1.144ValHis: 1.144 ± 0.04	4.61ValIle: 4.61 ± 0.083	4.109ValLys: 4.109 ± 0.075	6.175ValLeu: 6.175 ± 0.094	1.725ValMet: 1.725 ± 0.051	3.08ValAsn: 3.08 ± 0.07	3.031ValPro: 3.031 ± 0.064	1.638ValGln: 1.638 ± 0.046	3.661ValArg: 3.661 ± 0.074	5.51ValSer: 5.51 ± 0.108	4.051ValThr: 4.051 ± 0.091	4.893ValVal: 4.893 ± 0.087	0.728ValTrp: 0.728 ± 0.03	2.736ValTyr: 2.736 ± 0.067	0.0ValXaa: 0.0 ± 0.0
Trp	0.843TrpAla: 0.843 ± 0.033	0.175TrpCys: 0.175 ± 0.014	0.73TrpAsp: 0.73 ± 0.03	0.712TrpGlu: 0.712 ± 0.032	0.577TrpPhe: 0.577 ± 0.033	0.943TrpGly: 0.943 ± 0.035	0.225TrpHis: 0.225 ± 0.018	0.797TrpIle: 0.797 ± 0.037	0.83TrpLys: 0.83 ± 0.033	1.097TrpLeu: 1.097 ± 0.043	0.38TrpMet: 0.38 ± 0.023	0.77TrpAsn: 0.77 ± 0.035	0.332TrpPro: 0.332 ± 0.02	0.413TrpGln: 0.413 ± 0.024	0.568TrpArg: 0.568 ± 0.024	0.834TrpSer: 0.834 ± 0.038	0.739TrpThr: 0.739 ± 0.038	0.689TrpVal: 0.689 ± 0.032	0.24TrpTrp: 0.24 ± 0.019	0.546TrpTyr: 0.546 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.069TyrAla: 3.069 ± 0.067	0.536TyrCys: 0.536 ± 0.027	3.134TyrAsp: 3.134 ± 0.076	2.485TyrGlu: 2.485 ± 0.066	2.025TyrPhe: 2.025 ± 0.052	3.285TyrGly: 3.285 ± 0.071	0.76TyrHis: 0.76 ± 0.029	2.516TyrIle: 2.516 ± 0.057	2.474TyrLys: 2.474 ± 0.064	3.591TyrLeu: 3.591 ± 0.069	1.041TyrMet: 1.041 ± 0.041	2.145TyrAsn: 2.145 ± 0.07	1.827TyrPro: 1.827 ± 0.051	1.059TyrGln: 1.059 ± 0.039	2.314TyrArg: 2.314 ± 0.063	3.188TyrSer: 3.188 ± 0.084	2.361TyrThr: 2.361 ± 0.077	2.667TyrVal: 2.667 ± 0.064	0.478TyrTrp: 0.478 ± 0.027	2.079TyrTyr: 2.079 ± 0.068	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1918 proteins (741257 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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