Amino acid dipepetide frequency for Caulobacter sp. Root1455

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.503AlaAla: 21.503 ± 0.204	1.188AlaCys: 1.188 ± 0.03	7.782AlaAsp: 7.782 ± 0.075	7.183AlaGlu: 7.183 ± 0.081	4.969AlaPhe: 4.969 ± 0.061	12.193AlaGly: 12.193 ± 0.134	2.246AlaHis: 2.246 ± 0.04	5.511AlaIle: 5.511 ± 0.067	4.851AlaLys: 4.851 ± 0.084	14.642AlaLeu: 14.642 ± 0.14	3.406AlaMet: 3.406 ± 0.06	2.962AlaAsn: 2.962 ± 0.055	7.955AlaPro: 7.955 ± 0.12	4.759AlaGln: 4.759 ± 0.057	9.764AlaArg: 9.764 ± 0.114	6.707AlaSer: 6.707 ± 0.082	6.383AlaThr: 6.383 ± 0.083	10.273AlaVal: 10.273 ± 0.098	2.107AlaTrp: 2.107 ± 0.048	2.979AlaTyr: 2.979 ± 0.049	0.001AlaXaa: 0.001 ± 0.001
Cys	1.035CysAla: 1.035 ± 0.028	0.078CysCys: 0.078 ± 0.007	0.491CysAsp: 0.491 ± 0.021	0.398CysGlu: 0.398 ± 0.019	0.242CysPhe: 0.242 ± 0.014	0.84CysGly: 0.84 ± 0.024	0.166CysHis: 0.166 ± 0.01	0.261CysIle: 0.261 ± 0.014	0.201CysLys: 0.201 ± 0.013	0.687CysLeu: 0.687 ± 0.021	0.121CysMet: 0.121 ± 0.008	0.172CysAsn: 0.172 ± 0.013	0.402CysPro: 0.402 ± 0.017	0.203CysGln: 0.203 ± 0.011	0.476CysArg: 0.476 ± 0.021	0.348CysSer: 0.348 ± 0.016	0.326CysThr: 0.326 ± 0.018	0.628CysVal: 0.628 ± 0.024	0.117CysTrp: 0.117 ± 0.009	0.161CysTyr: 0.161 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.678AspAla: 7.678 ± 0.087	0.418AspCys: 0.418 ± 0.018	3.468AspAsp: 3.468 ± 0.063	3.209AspGlu: 3.209 ± 0.053	2.252AspPhe: 2.252 ± 0.052	5.856AspGly: 5.856 ± 0.082	1.335AspHis: 1.335 ± 0.036	2.44AspIle: 2.44 ± 0.047	1.974AspLys: 1.974 ± 0.04	6.679AspLeu: 6.679 ± 0.082	1.127AspMet: 1.127 ± 0.027	1.321AspAsn: 1.321 ± 0.036	4.104AspPro: 4.104 ± 0.067	2.05AspGln: 2.05 ± 0.043	4.321AspArg: 4.321 ± 0.06	2.148AspSer: 2.148 ± 0.045	2.533AspThr: 2.533 ± 0.051	4.232AspVal: 4.232 ± 0.051	1.064AspTrp: 1.064 ± 0.032	1.596AspTyr: 1.596 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	8.171GluAla: 8.171 ± 0.095	0.233GluCys: 0.233 ± 0.013	2.683GluAsp: 2.683 ± 0.05	2.349GluGlu: 2.349 ± 0.054	1.449GluPhe: 1.449 ± 0.033	4.473GluGly: 4.473 ± 0.062	1.059GluHis: 1.059 ± 0.031	2.801GluIle: 2.801 ± 0.047	1.848GluLys: 1.848 ± 0.048	4.529GluLeu: 4.529 ± 0.063	1.123GluMet: 1.123 ± 0.03	1.224GluAsn: 1.224 ± 0.033	2.704GluPro: 2.704 ± 0.054	1.784GluGln: 1.784 ± 0.042	4.534GluArg: 4.534 ± 0.07	2.037GluSer: 2.037 ± 0.034	3.41GluThr: 3.41 ± 0.049	3.642GluVal: 3.642 ± 0.055	0.566GluTrp: 0.566 ± 0.018	0.86GluTyr: 0.86 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.603PheAla: 4.603 ± 0.053	0.347PheCys: 0.347 ± 0.015	2.797PheAsp: 2.797 ± 0.046	2.131PheGlu: 2.131 ± 0.039	1.211PhePhe: 1.211 ± 0.033	3.66PheGly: 3.66 ± 0.049	0.771PheHis: 0.771 ± 0.025	1.329PheIle: 1.329 ± 0.033	1.19PheLys: 1.19 ± 0.03	3.001PheLeu: 3.001 ± 0.049	0.749PheMet: 0.749 ± 0.022	1.111PheAsn: 1.111 ± 0.031	1.537PhePro: 1.537 ± 0.037	1.058PheGln: 1.058 ± 0.027	1.997PheArg: 1.997 ± 0.04	2.102PheSer: 2.102 ± 0.043	2.055PheThr: 2.055 ± 0.043	2.7PheVal: 2.7 ± 0.044	0.556PheTrp: 0.556 ± 0.021	0.878PheTyr: 0.878 ± 0.026	0.001PheXaa: 0.001 ± 0.001
Gly	11.633GlyAla: 11.633 ± 0.13	0.759GlyCys: 0.759 ± 0.026	5.23GlyAsp: 5.23 ± 0.09	4.993GlyGlu: 4.993 ± 0.07	3.728GlyPhe: 3.728 ± 0.058	9.206GlyGly: 9.206 ± 0.252	1.677GlyHis: 1.677 ± 0.032	2.726GlyIle: 2.726 ± 0.045	3.453GlyLys: 3.453 ± 0.052	9.988GlyLeu: 9.988 ± 0.09	2.015GlyMet: 2.015 ± 0.042	1.683GlyAsn: 1.683 ± 0.044	4.447GlyPro: 4.447 ± 0.056	3.361GlyGln: 3.361 ± 0.047	6.34GlyArg: 6.34 ± 0.075	4.299GlySer: 4.299 ± 0.078	3.627GlyThr: 3.627 ± 0.086	8.139GlyVal: 8.139 ± 0.084	1.659GlyTrp: 1.659 ± 0.034	2.349GlyTyr: 2.349 ± 0.043	0.001GlyXaa: 0.001 ± 0.001
His	2.385HisAla: 2.385 ± 0.04	0.162HisCys: 0.162 ± 0.009	1.197HisAsp: 1.197 ± 0.028	0.913HisGlu: 0.913 ± 0.024	0.638HisPhe: 0.638 ± 0.021	1.953HisGly: 1.953 ± 0.041	0.505HisHis: 0.505 ± 0.02	0.673HisIle: 0.673 ± 0.024	0.457HisLys: 0.457 ± 0.016	1.831HisLeu: 1.831 ± 0.039	0.415HisMet: 0.415 ± 0.016	0.374HisAsn: 0.374 ± 0.013	1.258HisPro: 1.258 ± 0.03	0.572HisGln: 0.572 ± 0.019	1.25HisArg: 1.25 ± 0.031	0.667HisSer: 0.667 ± 0.021	0.707HisThr: 0.707 ± 0.022	1.405HisVal: 1.405 ± 0.032	0.301HisTrp: 0.301 ± 0.015	0.508HisTyr: 0.508 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.139IleAla: 6.139 ± 0.081	0.389IleCys: 0.389 ± 0.02	3.258IleAsp: 3.258 ± 0.053	2.842IleGlu: 2.842 ± 0.049	1.34IlePhe: 1.34 ± 0.032	4.253IleGly: 4.253 ± 0.061	0.771IleHis: 0.771 ± 0.025	1.489IleIle: 1.489 ± 0.04	1.35IleLys: 1.35 ± 0.035	3.788IleLeu: 3.788 ± 0.056	0.704IleMet: 0.704 ± 0.021	1.192IleAsn: 1.192 ± 0.035	1.939IlePro: 1.939 ± 0.036	1.214IleGln: 1.214 ± 0.03	2.516IleArg: 2.516 ± 0.045	2.284IleSer: 2.284 ± 0.042	2.288IleThr: 2.288 ± 0.046	3.526IleVal: 3.526 ± 0.046	0.537IleTrp: 0.537 ± 0.021	0.907IleTyr: 0.907 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	5.419LysAla: 5.419 ± 0.08	0.114LysCys: 0.114 ± 0.009	1.887LysAsp: 1.887 ± 0.04	1.125LysGlu: 1.125 ± 0.032	0.95LysPhe: 0.95 ± 0.026	2.953LysGly: 2.953 ± 0.052	0.526LysHis: 0.526 ± 0.019	1.66LysIle: 1.66 ± 0.039	1.119LysLys: 1.119 ± 0.037	3.202LysLeu: 3.202 ± 0.051	0.698LysMet: 0.698 ± 0.023	0.791LysAsn: 0.791 ± 0.026	2.442LysPro: 2.442 ± 0.047	0.821LysGln: 0.821 ± 0.023	2.194LysArg: 2.194 ± 0.046	1.742LysSer: 1.742 ± 0.036	2.307LysThr: 2.307 ± 0.041	2.724LysVal: 2.724 ± 0.046	0.359LysTrp: 0.359 ± 0.016	0.568LysTyr: 0.568 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.049LeuAla: 15.049 ± 0.143	0.742LeuCys: 0.742 ± 0.024	6.258LeuAsp: 6.258 ± 0.078	5.167LeuGlu: 5.167 ± 0.071	3.41LeuPhe: 3.41 ± 0.056	8.352LeuGly: 8.352 ± 0.08	1.676LeuHis: 1.676 ± 0.034	4.656LeuIle: 4.656 ± 0.062	3.943LeuLys: 3.943 ± 0.058	8.85LeuLeu: 8.85 ± 0.107	2.132LeuMet: 2.132 ± 0.039	2.679LeuAsn: 2.679 ± 0.042	5.167LeuPro: 5.167 ± 0.07	2.419LeuGln: 2.419 ± 0.04	6.548LeuArg: 6.548 ± 0.082	6.065LeuSer: 6.065 ± 0.069	6.253LeuThr: 6.253 ± 0.085	7.408LeuVal: 7.408 ± 0.078	1.256LeuTrp: 1.256 ± 0.036	2.058LeuTyr: 2.058 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.13MetAla: 3.13 ± 0.053	0.134MetCys: 0.134 ± 0.009	1.133MetAsp: 1.133 ± 0.031	0.881MetGlu: 0.881 ± 0.026	0.62MetPhe: 0.62 ± 0.021	1.805MetGly: 1.805 ± 0.042	0.342MetHis: 0.342 ± 0.015	1.132MetIle: 1.132 ± 0.033	0.84MetLys: 0.84 ± 0.027	2.018MetLeu: 2.018 ± 0.033	0.52MetMet: 0.52 ± 0.019	0.61MetAsn: 0.61 ± 0.024	1.157MetPro: 1.157 ± 0.029	0.552MetGln: 0.552 ± 0.018	1.48MetArg: 1.48 ± 0.033	1.505MetSer: 1.505 ± 0.03	1.841MetThr: 1.841 ± 0.033	1.467MetVal: 1.467 ± 0.033	0.186MetTrp: 0.186 ± 0.01	0.246MetTyr: 0.246 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.057AsnAla: 3.057 ± 0.049	0.201AsnCys: 0.201 ± 0.012	1.357AsnAsp: 1.357 ± 0.035	0.934AsnGlu: 0.934 ± 0.026	0.864AsnPhe: 0.864 ± 0.027	2.399AsnGly: 2.399 ± 0.054	0.456AsnHis: 0.456 ± 0.018	1.125AsnIle: 1.125 ± 0.03	0.596AsnLys: 0.596 ± 0.021	2.55AsnLeu: 2.55 ± 0.047	0.457AsnMet: 0.457 ± 0.017	0.661AsnAsn: 0.661 ± 0.027	1.753AsnPro: 1.753 ± 0.045	0.735AsnGln: 0.735 ± 0.025	1.595AsnArg: 1.595 ± 0.032	1.083AsnSer: 1.083 ± 0.032	1.25AsnThr: 1.25 ± 0.037	1.799AsnVal: 1.799 ± 0.041	0.387AsnTrp: 0.387 ± 0.017	0.675AsnTyr: 0.675 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.802ProAla: 7.802 ± 0.107	0.312ProCys: 0.312 ± 0.013	3.904ProAsp: 3.904 ± 0.054	3.335ProGlu: 3.335 ± 0.049	2.031ProPhe: 2.031 ± 0.035	5.055ProGly: 5.055 ± 0.067	0.961ProHis: 0.961 ± 0.027	2.312ProIle: 2.312 ± 0.041	1.932ProLys: 1.932 ± 0.037	4.949ProLeu: 4.949 ± 0.063	1.182ProMet: 1.182 ± 0.027	1.352ProAsn: 1.352 ± 0.037	3.583ProPro: 3.583 ± 0.079	1.627ProGln: 1.627 ± 0.038	3.413ProArg: 3.413 ± 0.062	3.028ProSer: 3.028 ± 0.053	3.079ProThr: 3.079 ± 0.059	4.34ProVal: 4.34 ± 0.061	0.804ProTrp: 0.804 ± 0.026	1.178ProTyr: 1.178 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.964GlnAla: 4.964 ± 0.056	0.162GlnCys: 0.162 ± 0.01	1.542GlnAsp: 1.542 ± 0.033	1.231GlnGlu: 1.231 ± 0.032	1.008GlnPhe: 1.008 ± 0.028	2.739GlnGly: 2.739 ± 0.048	0.528GlnHis: 0.528 ± 0.019	1.567GlnIle: 1.567 ± 0.032	0.952GlnLys: 0.952 ± 0.029	2.746GlnLeu: 2.746 ± 0.043	0.744GlnMet: 0.744 ± 0.022	0.709GlnAsn: 0.709 ± 0.025	1.759GlnPro: 1.759 ± 0.037	0.993GlnGln: 0.993 ± 0.029	2.204GlnArg: 2.204 ± 0.04	1.583GlnSer: 1.583 ± 0.04	1.992GlnThr: 1.992 ± 0.04	2.557GlnVal: 2.557 ± 0.042	0.407GlnTrp: 0.407 ± 0.017	0.549GlnTyr: 0.549 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.832ArgAla: 8.832 ± 0.087	0.44ArgCys: 0.44 ± 0.018	4.172ArgAsp: 4.172 ± 0.058	3.719ArgGlu: 3.719 ± 0.062	2.829ArgPhe: 2.829 ± 0.044	5.108ArgGly: 5.108 ± 0.066	1.365ArgHis: 1.365 ± 0.031	3.28ArgIle: 3.28 ± 0.044	2.212ArgLys: 2.212 ± 0.044	8.145ArgLeu: 8.145 ± 0.105	1.657ArgMet: 1.657 ± 0.035	1.467ArgAsn: 1.467 ± 0.032	4.018ArgPro: 4.018 ± 0.056	2.308ArgGln: 2.308 ± 0.041	5.727ArgArg: 5.727 ± 0.088	3.083ArgSer: 3.083 ± 0.053	3.404ArgThr: 3.404 ± 0.054	4.739ArgVal: 4.739 ± 0.068	1.123ArgTrp: 1.123 ± 0.032	1.735ArgTyr: 1.735 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	6.411SerAla: 6.411 ± 0.072	0.354SerCys: 0.354 ± 0.017	2.917SerAsp: 2.917 ± 0.055	2.255SerGlu: 2.255 ± 0.042	1.949SerPhe: 1.949 ± 0.04	5.175SerGly: 5.175 ± 0.088	0.907SerHis: 0.907 ± 0.029	2.239SerIle: 2.239 ± 0.043	1.565SerLys: 1.565 ± 0.034	5.261SerLeu: 5.261 ± 0.064	1.05SerMet: 1.05 ± 0.028	1.297SerAsn: 1.297 ± 0.034	3.119SerPro: 3.119 ± 0.05	1.638SerGln: 1.638 ± 0.035	3.315SerArg: 3.315 ± 0.052	2.534SerSer: 2.534 ± 0.053	2.741SerThr: 2.741 ± 0.054	3.756SerVal: 3.756 ± 0.06	0.766SerTrp: 0.766 ± 0.027	1.212SerTyr: 1.212 ± 0.032	0.001SerXaa: 0.001 ± 0.001
Thr	7.078ThrAla: 7.078 ± 0.095	0.372ThrCys: 0.372 ± 0.015	2.84ThrAsp: 2.84 ± 0.048	2.175ThrGlu: 2.175 ± 0.044	2.065ThrPhe: 2.065 ± 0.042	5.405ThrGly: 5.405 ± 0.088	0.954ThrHis: 0.954 ± 0.026	2.515ThrIle: 2.515 ± 0.052	1.464ThrLys: 1.464 ± 0.039	5.912ThrLeu: 5.912 ± 0.081	0.955ThrMet: 0.955 ± 0.026	1.282ThrAsn: 1.282 ± 0.038	3.96ThrPro: 3.96 ± 0.064	1.531ThrGln: 1.531 ± 0.037	3.26ThrArg: 3.26 ± 0.047	2.662ThrSer: 2.662 ± 0.057	3.138ThrThr: 3.138 ± 0.069	4.382ThrVal: 4.382 ± 0.058	0.795ThrTrp: 0.795 ± 0.027	1.347ThrTyr: 1.347 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	10.147ValAla: 10.147 ± 0.106	0.638ValCys: 0.638 ± 0.019	4.547ValAsp: 4.547 ± 0.056	4.89ValGlu: 4.89 ± 0.063	2.811ValPhe: 2.811 ± 0.05	6.47ValGly: 6.47 ± 0.091	1.306ValHis: 1.306 ± 0.031	3.572ValIle: 3.572 ± 0.05	2.445ValLys: 2.445 ± 0.053	7.557ValLeu: 7.557 ± 0.076	1.783ValMet: 1.783 ± 0.033	1.973ValAsn: 1.973 ± 0.04	3.137ValPro: 3.137 ± 0.057	2.191ValGln: 2.191 ± 0.038	5.187ValArg: 5.187 ± 0.072	4.441ValSer: 4.441 ± 0.058	4.616ValThr: 4.616 ± 0.063	6.475ValVal: 6.475 ± 0.086	1.1ValTrp: 1.1 ± 0.025	1.544ValTyr: 1.544 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.528TrpAla: 1.528 ± 0.034	0.122TrpCys: 0.122 ± 0.009	0.725TrpAsp: 0.725 ± 0.025	0.561TrpGlu: 0.561 ± 0.02	0.579TrpPhe: 0.579 ± 0.024	1.074TrpGly: 1.074 ± 0.032	0.209TrpHis: 0.209 ± 0.012	0.726TrpIle: 0.726 ± 0.023	0.553TrpLys: 0.553 ± 0.022	1.676TrpLeu: 1.676 ± 0.041	0.351TrpMet: 0.351 ± 0.016	0.482TrpAsn: 0.482 ± 0.019	0.752TrpPro: 0.752 ± 0.023	0.402TrpGln: 0.402 ± 0.016	1.51TrpArg: 1.51 ± 0.034	0.994TrpSer: 0.994 ± 0.03	1.074TrpThr: 1.074 ± 0.029	0.832TrpVal: 0.832 ± 0.024	0.274TrpTrp: 0.274 ± 0.016	0.286TrpTyr: 0.286 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.8TyrAla: 2.8 ± 0.045	0.188TyrCys: 0.188 ± 0.011	1.617TyrAsp: 1.617 ± 0.04	1.19TyrGlu: 1.19 ± 0.029	0.84TyrPhe: 0.84 ± 0.025	2.342TyrGly: 2.342 ± 0.053	0.411TyrHis: 0.411 ± 0.018	0.712TyrIle: 0.712 ± 0.022	0.655TyrLys: 0.655 ± 0.024	2.075TyrLeu: 2.075 ± 0.04	0.408TyrMet: 0.408 ± 0.018	0.592TyrAsn: 0.592 ± 0.022	0.986TyrPro: 0.986 ± 0.03	0.717TyrGln: 0.717 ± 0.027	1.734TyrArg: 1.734 ± 0.033	1.149TyrSer: 1.149 ± 0.034	1.067TyrThr: 1.067 ± 0.034	1.806TyrVal: 1.806 ± 0.037	0.347TyrTrp: 0.347 ± 0.015	0.583TyrTyr: 0.583 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4531 proteins (1466033 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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