Ketogulonicigenium robustum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ketogulonicigenium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6P1L3|A0A1W6P1L3_9RHOB Flagellar basal body rod protein FlgB OS=Ketogulonicigenium robustum OX=92947 GN=flgC PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84ALLAAILGGAVLAGAAQADD21 pKa = 3.93GKK23 pKa = 10.84IFVPLPDD30 pKa = 4.09FTALEE35 pKa = 4.35GDD37 pKa = 3.83SARR40 pKa = 11.84AEE42 pKa = 4.17GLLRR46 pKa = 11.84EE47 pKa = 4.11AFMVNVLMMNCSGVTYY63 pKa = 9.31TEE65 pKa = 4.24EE66 pKa = 3.97MSSLVIDD73 pKa = 3.65SAYY76 pKa = 10.83LLGYY80 pKa = 9.93GVLDD84 pKa = 3.9LTTDD88 pKa = 3.56ALEE91 pKa = 4.57TGYY94 pKa = 8.53STPAFDD100 pKa = 6.17YY101 pKa = 11.01LDD103 pKa = 4.04TPNACHH109 pKa = 6.41NAQARR114 pKa = 11.84SDD116 pKa = 3.61KK117 pKa = 10.89LITVLEE123 pKa = 4.88GYY125 pKa = 9.45GGSVIAYY132 pKa = 9.12ADD134 pKa = 3.35QDD136 pKa = 3.45QAYY139 pKa = 9.37LDD141 pKa = 3.58WRR143 pKa = 11.84ALMDD147 pKa = 4.75EE148 pKa = 4.6IEE150 pKa = 4.03NN151 pKa = 3.8

Molecular weight: 16.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6P1Z9|A0A1W6P1Z9_9RHOB 30S ribosomal protein S5 OS=Ketogulonicigenium robustum OX=92947 GN=rpsE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2662

0

2662

806659

37

1513

303.0

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.375 ± 0.075

0.715 ± 0.013

5.983 ± 0.041

4.884 ± 0.051

3.634 ± 0.031

8.589 ± 0.039

2.002 ± 0.023

5.313 ± 0.032

2.667 ± 0.039

10.258 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.023

2.66 ± 0.028

5.291 ± 0.039

3.598 ± 0.029

6.646 ± 0.048

4.923 ± 0.031

5.698 ± 0.034

7.422 ± 0.042

1.35 ± 0.018

2.197 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski