Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Marburgvirus; Marburg marburgvirus

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P31352|L_MABVM RNA-directed RNA polymerase L OS=Lake Victoria marburgvirus (strain Musoke-80) OX=33727 GN=L PE=3 SV=2
MM1 pKa = 8.04DD2 pKa = 4.52LHH4 pKa = 7.48SLLEE8 pKa = 4.65LGTKK12 pKa = 7.93PTAPHH17 pKa = 5.24VRR19 pKa = 11.84NKK21 pKa = 10.41KK22 pKa = 10.42VILFDD27 pKa = 3.75TNHH30 pKa = 5.59QVSICNQIIDD40 pKa = 5.28AINSGIDD47 pKa = 3.76LGDD50 pKa = 3.75LLEE53 pKa = 5.28GGLLTLCVEE62 pKa = 4.95HH63 pKa = 6.82YY64 pKa = 10.8YY65 pKa = 11.25NSDD68 pKa = 3.0KK69 pKa = 11.56DD70 pKa = 3.74KK71 pKa = 11.39FNTSPVAKK79 pKa = 9.16YY80 pKa = 10.57LRR82 pKa = 11.84DD83 pKa = 3.24AGYY86 pKa = 10.74EE87 pKa = 3.69FDD89 pKa = 4.23VIKK92 pKa = 11.05NADD95 pKa = 3.2ATRR98 pKa = 11.84FLDD101 pKa = 3.53VSPNEE106 pKa = 3.86PHH108 pKa = 6.52YY109 pKa = 11.36SPLILALKK117 pKa = 8.55TLEE120 pKa = 4.17STEE123 pKa = 4.17SQRR126 pKa = 11.84GRR128 pKa = 11.84IGLFLSFCSLFLPKK142 pKa = 10.41LVVGDD147 pKa = 3.54RR148 pKa = 11.84ASIEE152 pKa = 3.82KK153 pKa = 10.41ALRR156 pKa = 11.84QVTVHH161 pKa = 5.56QEE163 pKa = 3.93QGIVTYY169 pKa = 9.9PNHH172 pKa = 6.71WLTTGHH178 pKa = 6.17MKK180 pKa = 10.61VIFGILRR187 pKa = 11.84SSFILKK193 pKa = 9.98FVLIHH198 pKa = 5.96QGVNLVTGHH207 pKa = 7.24DD208 pKa = 4.17AYY210 pKa = 11.14DD211 pKa = 4.07SIISNSVGQTRR222 pKa = 11.84FSGLLIVKK230 pKa = 8.21TVLEE234 pKa = 5.06FILQKK239 pKa = 9.7TDD241 pKa = 2.82SGVTLHH247 pKa = 6.57PLVRR251 pKa = 11.84TSKK254 pKa = 10.47VKK256 pKa = 11.14NEE258 pKa = 3.81VASFKK263 pKa = 10.58QALSNLARR271 pKa = 11.84HH272 pKa = 6.11GEE274 pKa = 4.08YY275 pKa = 10.89APFARR280 pKa = 11.84VLNLSGINNLEE291 pKa = 4.3HH292 pKa = 6.95GLYY295 pKa = 9.39PQLSAIALGVATAHH309 pKa = 6.58GSTLAGVNVGEE320 pKa = 4.64QYY322 pKa = 10.17QQLRR326 pKa = 11.84EE327 pKa = 4.07AAHH330 pKa = 6.03DD331 pKa = 4.04AEE333 pKa = 4.82VKK335 pKa = 9.37LQRR338 pKa = 11.84RR339 pKa = 11.84HH340 pKa = 4.63EE341 pKa = 4.21HH342 pKa = 6.06QEE344 pKa = 3.48IQAIAEE350 pKa = 4.16DD351 pKa = 3.91DD352 pKa = 3.91EE353 pKa = 4.29EE354 pKa = 5.24RR355 pKa = 11.84KK356 pKa = 9.94ILEE359 pKa = 4.01QFHH362 pKa = 5.72LQKK365 pKa = 9.98TEE367 pKa = 3.73ITHH370 pKa = 5.44SQTLAVLSQKK380 pKa = 10.21RR381 pKa = 11.84EE382 pKa = 3.81KK383 pKa = 10.42LARR386 pKa = 11.84LAAEE390 pKa = 3.79IEE392 pKa = 4.2NNIVEE397 pKa = 4.38DD398 pKa = 3.53QGFKK402 pKa = 10.11QSQNRR407 pKa = 11.84VSQSFLNDD415 pKa = 3.43PTPVEE420 pKa = 4.29VTVQARR426 pKa = 11.84PMNRR430 pKa = 11.84PTALPPPVDD439 pKa = 4.08DD440 pKa = 5.38KK441 pKa = 11.53IEE443 pKa = 4.31HH444 pKa = 6.83EE445 pKa = 4.33STEE448 pKa = 4.41DD449 pKa = 3.42SSSSSSFVDD458 pKa = 3.95LNDD461 pKa = 3.61PFALLNEE468 pKa = 4.95DD469 pKa = 4.71EE470 pKa = 4.81DD471 pKa = 4.44TLDD474 pKa = 4.23DD475 pKa = 3.78SVMIPGTTSRR485 pKa = 11.84EE486 pKa = 3.9FQGIPEE492 pKa = 4.81PPRR495 pKa = 11.84QSQDD499 pKa = 3.14LNNSQGKK506 pKa = 9.17QEE508 pKa = 4.49DD509 pKa = 4.06EE510 pKa = 3.96STNRR514 pKa = 11.84IKK516 pKa = 10.84KK517 pKa = 9.59QFLRR521 pKa = 11.84YY522 pKa = 9.42QEE524 pKa = 4.57LPPVQEE530 pKa = 4.9DD531 pKa = 4.01DD532 pKa = 3.58EE533 pKa = 5.27SEE535 pKa = 4.33YY536 pKa = 8.54TTDD539 pKa = 3.24SQEE542 pKa = 5.59SIDD545 pKa = 4.96QPGSDD550 pKa = 3.86NEE552 pKa = 4.3QGVDD556 pKa = 3.96LPPPPLYY563 pKa = 10.67AQEE566 pKa = 4.25KK567 pKa = 8.18RR568 pKa = 11.84QDD570 pKa = 4.51PIQHH574 pKa = 6.82PAANPQDD581 pKa = 3.85PFGSIGDD588 pKa = 3.74VNGDD592 pKa = 3.0ILEE595 pKa = 5.1PIRR598 pKa = 11.84SPSSPSAPQEE608 pKa = 3.81DD609 pKa = 3.79TRR611 pKa = 11.84MRR613 pKa = 11.84EE614 pKa = 4.22AYY616 pKa = 9.32EE617 pKa = 4.06LSPDD621 pKa = 3.66FTNDD625 pKa = 3.84EE626 pKa = 4.8DD627 pKa = 4.68NQQNWPQRR635 pKa = 11.84VVTKK639 pKa = 10.47KK640 pKa = 10.64GRR642 pKa = 11.84TFLYY646 pKa = 10.4PNDD649 pKa = 4.25LLQTNPPEE657 pKa = 4.24SLITALVEE665 pKa = 4.45EE666 pKa = 4.58YY667 pKa = 10.69QNPVSAKK674 pKa = 9.4EE675 pKa = 4.17LQADD679 pKa = 3.88WPDD682 pKa = 3.06MSFDD686 pKa = 3.66EE687 pKa = 4.47RR688 pKa = 11.84RR689 pKa = 11.84HH690 pKa = 4.6VAMNLL695 pKa = 3.25

Molecular weight: 77.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P35259|VP35_MABVM Polymerase cofactor VP35 OS=Lake Victoria marburgvirus (strain Musoke-80) OX=33727 GN=VP35 PE=1 SV=1
MM1 pKa = 6.29QQPRR5 pKa = 11.84GRR7 pKa = 11.84SRR9 pKa = 11.84TRR11 pKa = 11.84NHH13 pKa = 5.53QVTPTIYY20 pKa = 10.52HH21 pKa = 5.65EE22 pKa = 4.38TQLPSKK28 pKa = 9.74PHH30 pKa = 4.43YY31 pKa = 9.3TNYY34 pKa = 10.04HH35 pKa = 5.0PRR37 pKa = 11.84ARR39 pKa = 11.84SMSSTRR45 pKa = 11.84SSAEE49 pKa = 3.7SSPTNHH55 pKa = 6.78IPRR58 pKa = 11.84ARR60 pKa = 11.84PPSTFNLSKK69 pKa = 10.53PPPPPKK75 pKa = 10.56DD76 pKa = 2.85MCRR79 pKa = 11.84NMKK82 pKa = 10.06IGLPCADD89 pKa = 3.59PTCNRR94 pKa = 11.84DD95 pKa = 3.04HH96 pKa = 7.73DD97 pKa = 5.04LDD99 pKa = 3.86NLTNRR104 pKa = 11.84EE105 pKa = 4.3LLLLMARR112 pKa = 11.84KK113 pKa = 8.27MLPNTDD119 pKa = 2.85KK120 pKa = 10.91TFRR123 pKa = 11.84SPQDD127 pKa = 3.35CGSPSLSKK135 pKa = 10.84GLSKK139 pKa = 10.9DD140 pKa = 3.3KK141 pKa = 11.08QEE143 pKa = 3.93QTKK146 pKa = 10.92DD147 pKa = 3.38VLTLEE152 pKa = 4.31NLGHH156 pKa = 6.39ILSYY160 pKa = 9.29LHH162 pKa = 6.8RR163 pKa = 11.84SEE165 pKa = 4.91IGKK168 pKa = 9.78LDD170 pKa = 3.73EE171 pKa = 4.47TSLRR175 pKa = 11.84AALSLTCAGIRR186 pKa = 11.84KK187 pKa = 7.28TNRR190 pKa = 11.84SLINTMTEE198 pKa = 3.11LHH200 pKa = 6.53MNHH203 pKa = 6.48EE204 pKa = 4.89NLPQDD209 pKa = 3.27QNGVIKK215 pKa = 9.13QTYY218 pKa = 8.31TGIHH222 pKa = 6.7LDD224 pKa = 3.15KK225 pKa = 11.17GGQFEE230 pKa = 4.05AALWQGWDD238 pKa = 3.44KK239 pKa = 11.29RR240 pKa = 11.84SISLFVQAALYY251 pKa = 10.21VMNNIPCEE259 pKa = 4.11SSISVQASYY268 pKa = 11.72DD269 pKa = 3.75HH270 pKa = 7.22FILPQSQGKK279 pKa = 8.18GQQ281 pKa = 3.19

Molecular weight: 31.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4873

253

2331

696.1

78.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.459 ± 0.444

1.642 ± 0.364

5.007 ± 0.333

5.315 ± 0.41

4.207 ± 0.477

4.946 ± 0.413

2.873 ± 0.153

6.382 ± 0.389

5.52 ± 0.269

11.081 ± 0.69

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.826 ± 0.295

5.91 ± 0.462

5.582 ± 0.777

4.925 ± 0.391

4.638 ± 0.314

8.742 ± 0.497

6.628 ± 0.951

4.925 ± 0.604

1.231 ± 0.177

3.16 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski