Amino acid dipepetide frequency for Pseudomonas phage Phabio

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.679AlaAla: 5.679 ± 0.38
0.738AlaCys: 0.738 ± 0.099
4.398AlaAsp: 4.398 ± 0.228
4.1AlaGlu: 4.1 ± 0.273
2.881AlaPhe: 2.881 ± 0.154
4.223AlaGly: 4.223 ± 0.265
1.322AlaHis: 1.322 ± 0.114
4.705AlaIle: 4.705 ± 0.229
4.264AlaLys: 4.264 ± 0.225
6.161AlaLeu: 6.161 ± 0.24
2.542AlaMet: 2.542 ± 0.167
3.352AlaAsn: 3.352 ± 0.194
2.553AlaPro: 2.553 ± 0.214
2.532AlaGln: 2.532 ± 0.178
3.342AlaArg: 3.342 ± 0.172
3.598AlaSer: 3.598 ± 0.235
4.08AlaThr: 4.08 ± 0.206
4.808AlaVal: 4.808 ± 0.235
0.8AlaTrp: 0.8 ± 0.11
2.768AlaTyr: 2.768 ± 0.176
0.0AlaXaa: 0.0 ± 0.0
Cys
0.584CysAla: 0.584 ± 0.086
0.144CysCys: 0.144 ± 0.039
0.687CysAsp: 0.687 ± 0.082
0.677CysGlu: 0.677 ± 0.082
0.308CysPhe: 0.308 ± 0.064
0.543CysGly: 0.543 ± 0.066
0.174CysHis: 0.174 ± 0.041
0.564CysIle: 0.564 ± 0.069
0.748CysLys: 0.748 ± 0.081
0.687CysLeu: 0.687 ± 0.093
0.318CysMet: 0.318 ± 0.062
0.502CysAsn: 0.502 ± 0.07
0.328CysPro: 0.328 ± 0.07
0.41CysGln: 0.41 ± 0.077
0.574CysArg: 0.574 ± 0.082
0.543CysSer: 0.543 ± 0.087
0.738CysThr: 0.738 ± 0.106
0.656CysVal: 0.656 ± 0.088
0.164CysTrp: 0.164 ± 0.041
0.441CysTyr: 0.441 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
3.967AspAla: 3.967 ± 0.189
0.543AspCys: 0.543 ± 0.074
4.029AspAsp: 4.029 ± 0.232
4.326AspGlu: 4.326 ± 0.216
2.471AspPhe: 2.471 ± 0.165
4.223AspGly: 4.223 ± 0.213
1.302AspHis: 1.302 ± 0.112
4.469AspIle: 4.469 ± 0.211
4.131AspLys: 4.131 ± 0.233
5.925AspLeu: 5.925 ± 0.241
1.763AspMet: 1.763 ± 0.137
3.209AspAsn: 3.209 ± 0.196
3.106AspPro: 3.106 ± 0.168
2.512AspGln: 2.512 ± 0.146
3.127AspArg: 3.127 ± 0.216
3.342AspSer: 3.342 ± 0.199
4.203AspThr: 4.203 ± 0.262
4.213AspVal: 4.213 ± 0.222
1.066AspTrp: 1.066 ± 0.102
2.983AspTyr: 2.983 ± 0.173
0.0AspXaa: 0.0 ± 0.0
Glu
5.023GluAla: 5.023 ± 0.298
0.666GluCys: 0.666 ± 0.081
3.731GluAsp: 3.731 ± 0.204
5.054GluGlu: 5.054 ± 0.286
2.747GluPhe: 2.747 ± 0.171
4.121GluGly: 4.121 ± 0.201
1.63GluHis: 1.63 ± 0.123
4.357GluIle: 4.357 ± 0.194
3.311GluLys: 3.311 ± 0.224
6.94GluLeu: 6.94 ± 0.285
1.784GluMet: 1.784 ± 0.137
2.922GluAsn: 2.922 ± 0.148
2.276GluPro: 2.276 ± 0.167
2.676GluGln: 2.676 ± 0.152
3.506GluArg: 3.506 ± 0.225
3.352GluSer: 3.352 ± 0.182
4.131GluThr: 4.131 ± 0.219
4.715GluVal: 4.715 ± 0.221
1.107GluTrp: 1.107 ± 0.117
3.075GluTyr: 3.075 ± 0.166
0.0GluXaa: 0.0 ± 0.0
Phe
2.542PheAla: 2.542 ± 0.174
0.502PheCys: 0.502 ± 0.078
3.27PheAsp: 3.27 ± 0.196
2.635PheGlu: 2.635 ± 0.193
1.722PhePhe: 1.722 ± 0.138
2.87PheGly: 2.87 ± 0.18
0.902PheHis: 0.902 ± 0.099
2.573PheIle: 2.573 ± 0.159
3.004PheLys: 3.004 ± 0.175
2.768PheLeu: 2.768 ± 0.15
1.107PheMet: 1.107 ± 0.114
2.676PheAsn: 2.676 ± 0.208
1.538PhePro: 1.538 ± 0.125
1.476PheGln: 1.476 ± 0.136
1.609PheArg: 1.609 ± 0.133
2.44PheSer: 2.44 ± 0.17
2.706PheThr: 2.706 ± 0.154
2.747PheVal: 2.747 ± 0.169
0.461PheTrp: 0.461 ± 0.061
1.661PheTyr: 1.661 ± 0.139
0.0PheXaa: 0.0 ± 0.0
Gly
3.434GlyAla: 3.434 ± 0.301
0.605GlyCys: 0.605 ± 0.078
4.285GlyAsp: 4.285 ± 0.423
4.121GlyGlu: 4.121 ± 0.237
2.706GlyPhe: 2.706 ± 0.153
4.295GlyGly: 4.295 ± 0.332
1.189GlyHis: 1.189 ± 0.119
4.09GlyIle: 4.09 ± 0.198
4.336GlyLys: 4.336 ± 0.247
5.208GlyLeu: 5.208 ± 0.243
1.558GlyMet: 1.558 ± 0.109
3.506GlyAsn: 3.506 ± 0.209
2.03GlyPro: 2.03 ± 0.281
2.03GlyGln: 2.03 ± 0.129
3.116GlyArg: 3.116 ± 0.202
3.916GlySer: 3.916 ± 0.179
4.039GlyThr: 4.039 ± 0.232
4.418GlyVal: 4.418 ± 0.241
0.974GlyTrp: 0.974 ± 0.104
2.963GlyTyr: 2.963 ± 0.196
0.0GlyXaa: 0.0 ± 0.0
His
1.189HisAla: 1.189 ± 0.123
0.308HisCys: 0.308 ± 0.054
1.107HisAsp: 1.107 ± 0.109
1.23HisGlu: 1.23 ± 0.107
0.789HisPhe: 0.789 ± 0.086
1.333HisGly: 1.333 ± 0.104
0.482HisHis: 0.482 ± 0.074
1.538HisIle: 1.538 ± 0.133
1.097HisLys: 1.097 ± 0.116
2.071HisLeu: 2.071 ± 0.144
0.472HisMet: 0.472 ± 0.067
1.179HisAsn: 1.179 ± 0.114
1.056HisPro: 1.056 ± 0.098
0.738HisGln: 0.738 ± 0.081
1.333HisArg: 1.333 ± 0.146
1.169HisSer: 1.169 ± 0.099
1.138HisThr: 1.138 ± 0.113
1.497HisVal: 1.497 ± 0.122
0.318HisTrp: 0.318 ± 0.052
1.148HisTyr: 1.148 ± 0.113
0.0HisXaa: 0.0 ± 0.0
Ile
4.418IleAla: 4.418 ± 0.259
0.646IleCys: 0.646 ± 0.076
5.003IleAsp: 5.003 ± 0.232
4.357IleGlu: 4.357 ± 0.224
1.917IlePhe: 1.917 ± 0.147
3.701IleGly: 3.701 ± 0.199
1.292IleHis: 1.292 ± 0.114
3.178IleIle: 3.178 ± 0.185
4.234IleLys: 4.234 ± 0.189
4.213IleLeu: 4.213 ± 0.226
1.517IleMet: 1.517 ± 0.138
3.731IleAsn: 3.731 ± 0.163
3.116IlePro: 3.116 ± 0.174
2.286IleGln: 2.286 ± 0.153
3.332IleArg: 3.332 ± 0.191
3.649IleSer: 3.649 ± 0.213
4.941IleThr: 4.941 ± 0.211
4.049IleVal: 4.049 ± 0.229
0.759IleTrp: 0.759 ± 0.089
2.194IleTyr: 2.194 ± 0.167
0.0IleXaa: 0.0 ± 0.0
Lys
4.962LysAla: 4.962 ± 0.248
0.502LysCys: 0.502 ± 0.07
4.193LysAsp: 4.193 ± 0.228
4.633LysGlu: 4.633 ± 0.251
2.368LysPhe: 2.368 ± 0.156
4.008LysGly: 4.008 ± 0.44
1.558LysHis: 1.558 ± 0.158
3.168LysIle: 3.168 ± 0.193
3.198LysLys: 3.198 ± 0.208
5.525LysLeu: 5.525 ± 0.248
1.62LysMet: 1.62 ± 0.131
2.296LysAsn: 2.296 ± 0.146
2.727LysPro: 2.727 ± 0.173
2.45LysGln: 2.45 ± 0.14
3.198LysArg: 3.198 ± 0.202
2.973LysSer: 2.973 ± 0.16
3.383LysThr: 3.383 ± 0.19
4.756LysVal: 4.756 ± 0.204
0.841LysTrp: 0.841 ± 0.093
2.614LysTyr: 2.614 ± 0.151
0.0LysXaa: 0.0 ± 0.0
Leu
5.628LeuAla: 5.628 ± 0.232
0.871LeuCys: 0.871 ± 0.092
5.484LeuAsp: 5.484 ± 0.221
6.397LeuGlu: 6.397 ± 0.29
3.608LeuPhe: 3.608 ± 0.186
5.382LeuGly: 5.382 ± 0.273
2.03LeuHis: 2.03 ± 0.135
4.838LeuIle: 4.838 ± 0.207
5.638LeuLys: 5.638 ± 0.254
6.356LeuLeu: 6.356 ± 0.313
2.132LeuMet: 2.132 ± 0.145
4.633LeuAsn: 4.633 ± 0.247
3.906LeuPro: 3.906 ± 0.171
3.055LeuGln: 3.055 ± 0.167
4.326LeuArg: 4.326 ± 0.199
5.044LeuSer: 5.044 ± 0.218
5.392LeuThr: 5.392 ± 0.25
5.782LeuVal: 5.782 ± 0.216
0.953LeuTrp: 0.953 ± 0.084
2.922LeuTyr: 2.922 ± 0.195
0.0LeuXaa: 0.0 ± 0.0
Met
2.173MetAla: 2.173 ± 0.148
0.215MetCys: 0.215 ± 0.056
1.517MetAsp: 1.517 ± 0.118
1.476MetGlu: 1.476 ± 0.127
1.343MetPhe: 1.343 ± 0.131
1.476MetGly: 1.476 ± 0.134
0.728MetHis: 0.728 ± 0.08
1.507MetIle: 1.507 ± 0.123
1.661MetLys: 1.661 ± 0.134
2.235MetLeu: 2.235 ± 0.161
0.677MetMet: 0.677 ± 0.095
1.507MetAsn: 1.507 ± 0.12
0.943MetPro: 0.943 ± 0.084
0.923MetGln: 0.923 ± 0.104
1.507MetArg: 1.507 ± 0.137
2.358MetSer: 2.358 ± 0.138
2.05MetThr: 2.05 ± 0.134
2.101MetVal: 2.101 ± 0.152
0.236MetTrp: 0.236 ± 0.047
1.128MetTyr: 1.128 ± 0.1
0.0MetXaa: 0.0 ± 0.0
Asn
3.403AsnAla: 3.403 ± 0.226
0.4AsnCys: 0.4 ± 0.069
2.911AsnAsp: 2.911 ± 0.178
2.942AsnGlu: 2.942 ± 0.184
2.194AsnPhe: 2.194 ± 0.15
3.824AsnGly: 3.824 ± 0.203
1.107AsnHis: 1.107 ± 0.106
3.321AsnIle: 3.321 ± 0.198
3.198AsnLys: 3.198 ± 0.182
4.059AsnLeu: 4.059 ± 0.213
1.517AsnMet: 1.517 ± 0.137
2.891AsnAsn: 2.891 ± 0.226
2.84AsnPro: 2.84 ± 0.167
2.019AsnGln: 2.019 ± 0.14
2.778AsnArg: 2.778 ± 0.182
2.952AsnSer: 2.952 ± 0.165
3.28AsnThr: 3.28 ± 0.187
3.352AsnVal: 3.352 ± 0.19
0.8AsnTrp: 0.8 ± 0.094
2.101AsnTyr: 2.101 ± 0.132
0.0AsnXaa: 0.0 ± 0.0
Pro
2.819ProAla: 2.819 ± 0.198
0.338ProCys: 0.338 ± 0.059
3.137ProAsp: 3.137 ± 0.163
3.66ProGlu: 3.66 ± 0.229
1.63ProPhe: 1.63 ± 0.126
2.594ProGly: 2.594 ± 0.173
0.81ProHis: 0.81 ± 0.095
2.183ProIle: 2.183 ± 0.163
2.45ProLys: 2.45 ± 0.181
3.055ProLeu: 3.055 ± 0.193
1.005ProMet: 1.005 ± 0.105
2.06ProAsn: 2.06 ± 0.16
1.363ProPro: 1.363 ± 0.107
1.128ProGln: 1.128 ± 0.14
1.845ProArg: 1.845 ± 0.138
2.655ProSer: 2.655 ± 0.146
2.983ProThr: 2.983 ± 0.196
3.332ProVal: 3.332 ± 0.185
0.513ProTrp: 0.513 ± 0.07
1.835ProTyr: 1.835 ± 0.128
0.0ProXaa: 0.0 ± 0.0
Gln
2.778GlnAla: 2.778 ± 0.188
0.4GlnCys: 0.4 ± 0.059
1.866GlnAsp: 1.866 ± 0.136
2.214GlnGlu: 2.214 ± 0.144
1.65GlnPhe: 1.65 ± 0.131
2.224GlnGly: 2.224 ± 0.279
0.83GlnHis: 0.83 ± 0.102
2.173GlnIle: 2.173 ± 0.143
1.466GlnLys: 1.466 ± 0.132
3.824GlnLeu: 3.824 ± 0.187
1.025GlnMet: 1.025 ± 0.093
1.784GlnAsn: 1.784 ± 0.158
1.527GlnPro: 1.527 ± 0.148
1.835GlnGln: 1.835 ± 0.23
2.06GlnArg: 2.06 ± 0.142
1.927GlnSer: 1.927 ± 0.135
2.173GlnThr: 2.173 ± 0.122
2.481GlnVal: 2.481 ± 0.165
0.492GlnTrp: 0.492 ± 0.069
1.609GlnTyr: 1.609 ± 0.132
0.0GlnXaa: 0.0 ± 0.0
Arg
3.444ArgAla: 3.444 ± 0.172
0.595ArgCys: 0.595 ± 0.082
3.168ArgAsp: 3.168 ± 0.192
3.526ArgGlu: 3.526 ± 0.191
2.419ArgPhe: 2.419 ± 0.158
2.635ArgGly: 2.635 ± 0.172
1.025ArgHis: 1.025 ± 0.093
3.034ArgIle: 3.034 ± 0.185
3.086ArgLys: 3.086 ± 0.192
4.623ArgLeu: 4.623 ± 0.242
1.507ArgMet: 1.507 ± 0.112
2.624ArgAsn: 2.624 ± 0.158
2.05ArgPro: 2.05 ± 0.149
1.948ArgGln: 1.948 ± 0.141
2.973ArgArg: 2.973 ± 0.183
2.819ArgSer: 2.819 ± 0.208
2.747ArgThr: 2.747 ± 0.149
3.68ArgVal: 3.68 ± 0.194
0.82ArgTrp: 0.82 ± 0.082
2.296ArgTyr: 2.296 ± 0.152
0.0ArgXaa: 0.0 ± 0.0
Ser
3.813SerAla: 3.813 ± 0.219
0.482SerCys: 0.482 ± 0.067
3.383SerAsp: 3.383 ± 0.18
3.393SerGlu: 3.393 ± 0.192
2.696SerPhe: 2.696 ± 0.179
3.916SerGly: 3.916 ± 0.188
0.994SerHis: 0.994 ± 0.106
3.957SerIle: 3.957 ± 0.195
3.455SerLys: 3.455 ± 0.176
4.931SerLeu: 4.931 ± 0.193
1.825SerMet: 1.825 ± 0.137
2.973SerAsn: 2.973 ± 0.175
2.327SerPro: 2.327 ± 0.152
1.784SerGln: 1.784 ± 0.143
2.901SerArg: 2.901 ± 0.18
3.219SerSer: 3.219 ± 0.181
3.844SerThr: 3.844 ± 0.16
4.029SerVal: 4.029 ± 0.196
0.615SerTrp: 0.615 ± 0.089
2.255SerTyr: 2.255 ± 0.164
0.0SerXaa: 0.0 ± 0.0
Thr
4.408ThrAla: 4.408 ± 0.278
0.554ThrCys: 0.554 ± 0.074
4.295ThrAsp: 4.295 ± 0.244
4.09ThrGlu: 4.09 ± 0.219
2.829ThrPhe: 2.829 ± 0.179
4.367ThrGly: 4.367 ± 0.24
1.251ThrHis: 1.251 ± 0.121
4.418ThrIle: 4.418 ± 0.212
3.629ThrLys: 3.629 ± 0.192
5.249ThrLeu: 5.249 ± 0.25
1.445ThrMet: 1.445 ± 0.134
3.25ThrAsn: 3.25 ± 0.208
3.014ThrPro: 3.014 ± 0.157
2.245ThrGln: 2.245 ± 0.14
3.034ThrArg: 3.034 ± 0.174
3.465ThrSer: 3.465 ± 0.21
4.254ThrThr: 4.254 ± 0.237
5.146ThrVal: 5.146 ± 0.226
0.902ThrTrp: 0.902 ± 0.09
2.706ThrTyr: 2.706 ± 0.185
0.0ThrXaa: 0.0 ± 0.0
Val
5.249ValAla: 5.249 ± 0.231
0.697ValCys: 0.697 ± 0.083
4.89ValAsp: 4.89 ± 0.224
4.797ValGlu: 4.797 ± 0.267
2.512ValPhe: 2.512 ± 0.169
4.223ValGly: 4.223 ± 0.228
1.261ValHis: 1.261 ± 0.13
4.859ValIle: 4.859 ± 0.216
4.828ValLys: 4.828 ± 0.221
5.71ValLeu: 5.71 ± 0.278
2.286ValMet: 2.286 ± 0.178
3.66ValAsn: 3.66 ± 0.225
2.696ValPro: 2.696 ± 0.155
2.347ValGln: 2.347 ± 0.158
3.362ValArg: 3.362 ± 0.194
4.295ValSer: 4.295 ± 0.22
5.023ValThr: 5.023 ± 0.25
5.566ValVal: 5.566 ± 0.242
0.81ValTrp: 0.81 ± 0.089
2.563ValTyr: 2.563 ± 0.165
0.0ValXaa: 0.0 ± 0.0
Trp
0.81TrpAla: 0.81 ± 0.099
0.164TrpCys: 0.164 ± 0.039
0.861TrpAsp: 0.861 ± 0.079
0.892TrpGlu: 0.892 ± 0.096
0.646TrpPhe: 0.646 ± 0.088
0.595TrpGly: 0.595 ± 0.077
0.226TrpHis: 0.226 ± 0.046
0.871TrpIle: 0.871 ± 0.103
0.759TrpLys: 0.759 ± 0.079
1.322TrpLeu: 1.322 ± 0.11
0.379TrpMet: 0.379 ± 0.059
0.81TrpAsn: 0.81 ± 0.08
0.297TrpPro: 0.297 ± 0.054
0.4TrpGln: 0.4 ± 0.061
0.656TrpArg: 0.656 ± 0.084
0.82TrpSer: 0.82 ± 0.089
0.82TrpThr: 0.82 ± 0.095
1.404TrpVal: 1.404 ± 0.131
0.144TrpTrp: 0.144 ± 0.041
0.482TrpTyr: 0.482 ± 0.077
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.522TyrAla: 2.522 ± 0.171
0.42TyrCys: 0.42 ± 0.063
2.696TyrAsp: 2.696 ± 0.149
2.604TyrGlu: 2.604 ± 0.152
1.773TyrPhe: 1.773 ± 0.14
2.265TyrGly: 2.265 ± 0.171
1.076TyrHis: 1.076 ± 0.131
2.727TyrIle: 2.727 ± 0.139
2.481TyrLys: 2.481 ± 0.16
3.526TyrLeu: 3.526 ± 0.217
1.271TyrMet: 1.271 ± 0.12
2.327TyrAsn: 2.327 ± 0.172
1.702TyrPro: 1.702 ± 0.127
1.548TyrGln: 1.548 ± 0.113
2.44TyrArg: 2.44 ± 0.157
2.255TyrSer: 2.255 ± 0.159
2.624TyrThr: 2.624 ± 0.157
2.901TyrVal: 2.901 ± 0.173
0.584TyrTrp: 0.584 ± 0.075
1.753TyrTyr: 1.753 ± 0.153
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 468 proteins (97552 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski