Pararge aegeria rhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Alphapaprhavirus; Pararge alphapaprhavirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140D8Q3|A0A140D8Q3_9RHAB GDP polyribonucleotidyltransferase OS=Pararge aegeria rhabdovirus OX=1802938 GN=L PE=4 SV=1
MM1 pKa = 7.45MICTLILIVVGFNNLPVSHH20 pKa = 7.0SSPRR24 pKa = 11.84DD25 pKa = 3.38FTSDD29 pKa = 3.26YY30 pKa = 11.26LLYY33 pKa = 9.2PVQMHH38 pKa = 6.32GSRR41 pKa = 11.84RR42 pKa = 11.84LASVDD47 pKa = 3.2RR48 pKa = 11.84LKK50 pKa = 11.02CPKK53 pKa = 10.51LSDD56 pKa = 3.56SLSKK60 pKa = 10.87VKK62 pKa = 10.7RR63 pKa = 11.84FDD65 pKa = 3.54LQLATAEE72 pKa = 3.82PRR74 pKa = 11.84TRR76 pKa = 11.84VGYY79 pKa = 10.22LCIGVTSDD87 pKa = 3.75IVCSSAILSYY97 pKa = 9.99ATSSSQKK104 pKa = 7.97TVHH107 pKa = 4.99VTKK110 pKa = 10.27SDD112 pKa = 3.73CEE114 pKa = 4.06VRR116 pKa = 11.84VSKK119 pKa = 10.76FSHH122 pKa = 5.68GQNTGFKK129 pKa = 8.7EE130 pKa = 4.32TTPDD134 pKa = 3.25CALGYY139 pKa = 9.34TSDD142 pKa = 4.26RR143 pKa = 11.84RR144 pKa = 11.84DD145 pKa = 3.13HH146 pKa = 6.31QIIVKK151 pKa = 9.84KK152 pKa = 10.53QIFPFSPLKK161 pKa = 10.71NGIVSSLFPGSVCADD176 pKa = 4.48RR177 pKa = 11.84YY178 pKa = 10.37CSSSDD183 pKa = 3.23GTTHH187 pKa = 7.3WFLEE191 pKa = 4.06SSGDD195 pKa = 3.75YY196 pKa = 11.09GCTGLVNGTGEE207 pKa = 4.48LKK209 pKa = 10.52ISTAGKK215 pKa = 10.25GRR217 pKa = 11.84SQLLSTSVTGDD228 pKa = 2.81IPLNEE233 pKa = 3.89ICKK236 pKa = 9.64TDD238 pKa = 4.08DD239 pKa = 3.3YY240 pKa = 11.41CGFPTYY246 pKa = 10.04IDD248 pKa = 3.49YY249 pKa = 11.36SFNGFSLTSLEE260 pKa = 3.94GDD262 pKa = 3.08IGSILAQDD270 pKa = 3.89VRR272 pKa = 11.84DD273 pKa = 4.07CPGGGSIRR281 pKa = 11.84EE282 pKa = 3.94YY283 pKa = 10.82HH284 pKa = 6.6PNDD287 pKa = 3.89LKK289 pKa = 10.66TMLSLTEE296 pKa = 4.64TIHH299 pKa = 5.6QRR301 pKa = 11.84HH302 pKa = 5.29YY303 pKa = 10.62QCQKK307 pKa = 9.74ILNKK311 pKa = 10.36AKK313 pKa = 10.66EE314 pKa = 4.17EE315 pKa = 4.28GFMSSRR321 pKa = 11.84YY322 pKa = 8.28LTLFQPYY329 pKa = 9.38QEE331 pKa = 5.38GIAPGYY337 pKa = 9.87RR338 pKa = 11.84LEE340 pKa = 4.74NGKK343 pKa = 9.34LWEE346 pKa = 4.08YY347 pKa = 11.2DD348 pKa = 3.13LYY350 pKa = 10.57YY351 pKa = 10.62TIGLFKK357 pKa = 10.83SQMIDD362 pKa = 2.79QTTYY366 pKa = 9.05KK367 pKa = 9.05TYY369 pKa = 10.07KK370 pKa = 9.01WDD372 pKa = 3.87VIDD375 pKa = 4.53ASGSFTTLPPSLCLFDD391 pKa = 3.55TTYY394 pKa = 11.18EE395 pKa = 4.01NSADD399 pKa = 3.74GPLCHH404 pKa = 6.94WFNGIQIHH412 pKa = 6.77GINLLYY418 pKa = 10.19PDD420 pKa = 3.4VANFFEE426 pKa = 4.53YY427 pKa = 10.64SHH429 pKa = 7.69DD430 pKa = 4.21YY431 pKa = 7.78PTLPYY436 pKa = 9.57STEE439 pKa = 4.12TTDD442 pKa = 4.12EE443 pKa = 4.29DD444 pKa = 4.35TDD446 pKa = 4.0LQHH449 pKa = 6.87NPTIPKK455 pKa = 9.53SWTGLLPFWGEE466 pKa = 3.97VLFLSISGVIFLLIIWLVLWSCCNGSKK493 pKa = 9.3TKK495 pKa = 10.6RR496 pKa = 11.84SGTEE500 pKa = 3.7TLNDD504 pKa = 3.83GGDD507 pKa = 3.59VKK509 pKa = 10.2ITEE512 pKa = 4.98LGTIRR517 pKa = 11.84PSLPQIRR524 pKa = 11.84QAIPLAWNQHH534 pKa = 5.48NIVTTRR540 pKa = 11.84HH541 pKa = 5.56SPPMLEE547 pKa = 4.16WFDD550 pKa = 3.65

Molecular weight: 61.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140D8Q1|A0A140D8Q1_9RHAB Glycoprotein 1 OS=Pararge aegeria rhabdovirus OX=1802938 GN=G1 PE=4 SV=1
MM1 pKa = 7.47SSSTSSSSSEE11 pKa = 3.9TKK13 pKa = 8.62KK14 pKa = 10.69QKK16 pKa = 9.48VRR18 pKa = 11.84RR19 pKa = 11.84QRR21 pKa = 11.84TFLGIRR27 pKa = 11.84WPRR30 pKa = 11.84QSSSSDD36 pKa = 3.15SLSPSEE42 pKa = 4.28GLNPLALPGRR52 pKa = 11.84SSGPTIIPSGFMMGGSVNWANPEE75 pKa = 3.59WDD77 pKa = 3.39FHH79 pKa = 7.9KK80 pKa = 11.07EE81 pKa = 3.83EE82 pKa = 4.24VSDD85 pKa = 4.0VVTILPFPCQVTCTLMIVSRR105 pKa = 11.84KK106 pKa = 9.23PISDD110 pKa = 3.62VQSLLRR116 pKa = 11.84KK117 pKa = 10.04LEE119 pKa = 3.99LWVDD123 pKa = 3.92QYY125 pKa = 11.77SGSYY129 pKa = 9.65VSRR132 pKa = 11.84PYY134 pKa = 9.85LTLIYY139 pKa = 10.0ILLGAKK145 pKa = 8.02LTSRR149 pKa = 11.84KK150 pKa = 8.41STSRR154 pKa = 11.84NYY156 pKa = 9.94EE157 pKa = 3.83YY158 pKa = 10.38HH159 pKa = 7.13SKK161 pKa = 9.63FQGVIRR167 pKa = 11.84FGIQSDD173 pKa = 3.98RR174 pKa = 11.84NKK176 pKa = 10.42PPAEE180 pKa = 3.93NFRR183 pKa = 11.84LVMSEE188 pKa = 3.92GLEE191 pKa = 3.84DD192 pKa = 3.14SYY194 pKa = 11.86YY195 pKa = 10.8AISFEE200 pKa = 4.84CYY202 pKa = 9.67SSEE205 pKa = 4.18SKK207 pKa = 10.61RR208 pKa = 11.84KK209 pKa = 9.14GVIYY213 pKa = 10.6SDD215 pKa = 4.06LYY217 pKa = 11.06KK218 pKa = 10.5MNGYY222 pKa = 10.27HH223 pKa = 5.28YY224 pKa = 7.96TFPCAMEE231 pKa = 4.41LLGLQVVMDD240 pKa = 3.99QEE242 pKa = 4.45GLIASAA248 pKa = 4.71

Molecular weight: 27.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4246

248

2135

707.7

80.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.91 ± 0.505

2.002 ± 0.313

5.417 ± 0.343

5.37 ± 0.369

3.745 ± 0.228

7.065 ± 0.345

2.591 ± 0.176

7.042 ± 0.547

5.582 ± 0.297

11.187 ± 0.83

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.302

4.31 ± 0.308

5.252 ± 0.171

3.58 ± 0.164

5.535 ± 0.391

8.455 ± 0.893

5.888 ± 0.545

5.134 ± 0.268

1.743 ± 0.092

3.98 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski