Tai Forest reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Coltivirus; Tai Forest coltivirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291B0C8|A0A291B0C8_9REOV Uncharacterized protein OS=Tai Forest reovirus OX=2039230 PE=4 SV=1
SS1 pKa = 7.29SIRR4 pKa = 11.84LTGDD8 pKa = 4.0TINHH12 pKa = 5.8GLEE15 pKa = 4.68SIAATHH21 pKa = 7.62DD22 pKa = 3.37DD23 pKa = 3.57RR24 pKa = 11.84QFLVEE29 pKa = 4.65LSGKK33 pKa = 9.67HH34 pKa = 4.98VGRR37 pKa = 11.84IQDD40 pKa = 3.29LWSRR44 pKa = 11.84SLPEE48 pKa = 3.44GWLFQVRR55 pKa = 11.84GKK57 pKa = 9.9LFCFIPIDD65 pKa = 3.34IPLFVHH71 pKa = 6.7EE72 pKa = 5.56FYY74 pKa = 10.66QEE76 pKa = 3.63KK77 pKa = 10.31GVIFVRR83 pKa = 11.84VNGLDD88 pKa = 5.44DD89 pKa = 3.79MLSWWAEE96 pKa = 3.5FDD98 pKa = 3.4PAEE101 pKa = 4.68RR102 pKa = 11.84IIHH105 pKa = 5.92CEE107 pKa = 3.65SYY109 pKa = 11.41LNDD112 pKa = 3.09EE113 pKa = 4.08LAYY116 pKa = 10.98NRR118 pKa = 11.84VALTQGRR125 pKa = 11.84AIMFEE130 pKa = 3.88LARR133 pKa = 11.84RR134 pKa = 11.84EE135 pKa = 3.99RR136 pKa = 11.84DD137 pKa = 3.51TVILDD142 pKa = 2.96IHH144 pKa = 6.99ALIIQNAAAAIQGVDD159 pKa = 3.58ADD161 pKa = 4.61DD162 pKa = 4.47MYY164 pKa = 11.66SGLDD168 pKa = 3.57GIIPVRR174 pKa = 11.84DD175 pKa = 3.26EE176 pKa = 3.84GAFRR180 pKa = 11.84GGGPLRR186 pKa = 11.84PAILPGAVAQPPVAGVRR203 pKa = 11.84LKK205 pKa = 10.72ALPPLPVADD214 pKa = 4.05EE215 pKa = 4.19NGLRR219 pKa = 11.84EE220 pKa = 4.36GEE222 pKa = 4.17VDD224 pKa = 4.33LLAHH228 pKa = 5.66QVSNLQVQQGIQQAAAGPQVQDD250 pKa = 3.57VVVDD254 pKa = 3.87PMNVPLPDD262 pKa = 4.83DD263 pKa = 4.37DD264 pKa = 5.51EE265 pKa = 5.98FMDD268 pKa = 3.93VAPAEE273 pKa = 4.35PPQVIPAADD282 pKa = 3.42VAVEE286 pKa = 3.83RR287 pKa = 11.84RR288 pKa = 11.84MPPVVQNVALQVDD301 pKa = 4.63VPPLNDD307 pKa = 3.32RR308 pKa = 11.84ANYY311 pKa = 9.89RR312 pKa = 11.84LRR314 pKa = 11.84NLPYY318 pKa = 10.42QNDD321 pKa = 3.65QIRR324 pKa = 11.84RR325 pKa = 11.84KK326 pKa = 9.12YY327 pKa = 10.38EE328 pKa = 3.62EE329 pKa = 4.23YY330 pKa = 10.82EE331 pKa = 4.52DD332 pKa = 3.51IFEE335 pKa = 4.43TMRR338 pKa = 11.84GAVEE342 pKa = 3.83DD343 pKa = 3.8ANRR346 pKa = 11.84YY347 pKa = 8.56INNIQDD353 pKa = 3.87DD354 pKa = 4.56VLTLEE359 pKa = 4.26NLRR362 pKa = 11.84NIPQAFQILEE372 pKa = 4.1RR373 pKa = 11.84FVVRR377 pKa = 11.84NTARR381 pKa = 11.84LDD383 pKa = 3.52PFRR386 pKa = 11.84LPSSTLNEE394 pKa = 4.23TILEE398 pKa = 4.07QEE400 pKa = 4.19YY401 pKa = 11.1GLLHH405 pKa = 7.66DD406 pKa = 3.97EE407 pKa = 4.57VAWVRR412 pKa = 11.84DD413 pKa = 3.75VGPEE417 pKa = 3.79EE418 pKa = 4.18VDD420 pKa = 3.15PDD422 pKa = 3.9RR423 pKa = 11.84LDD425 pKa = 3.55EE426 pKa = 4.29QIRR429 pKa = 11.84DD430 pKa = 3.57YY431 pKa = 11.38DD432 pKa = 3.55EE433 pKa = 5.39RR434 pKa = 11.84LADD437 pKa = 3.77EE438 pKa = 4.36EE439 pKa = 4.27MALIEE444 pKa = 4.37AEE446 pKa = 4.25SRR448 pKa = 11.84ALEE451 pKa = 4.26AKK453 pKa = 10.19LMRR456 pKa = 11.84VGIPVGAGTGLEE468 pKa = 4.41TIRR471 pKa = 11.84VEE473 pKa = 4.09YY474 pKa = 10.95GMVGAIDD481 pKa = 3.61QGPYY485 pKa = 9.41IDD487 pKa = 5.99DD488 pKa = 3.83YY489 pKa = 12.21VEE491 pKa = 5.55DD492 pKa = 5.71DD493 pKa = 3.74VLSWTGFYY501 pKa = 10.91QNFSLDD507 pKa = 3.6KK508 pKa = 10.02VFHH511 pKa = 7.11DD512 pKa = 4.67FFSRR516 pKa = 11.84IPDD519 pKa = 3.24IPTGRR524 pKa = 11.84RR525 pKa = 11.84MLARR529 pKa = 11.84VMRR532 pKa = 11.84DD533 pKa = 2.83VQTRR537 pKa = 11.84VSTLTSVNRR546 pKa = 11.84EE547 pKa = 3.71RR548 pKa = 11.84FRR550 pKa = 11.84EE551 pKa = 3.96PNGIRR556 pKa = 11.84DD557 pKa = 3.65LAGVSIVPGTAFNMMLRR574 pKa = 11.84WSTLSARR581 pKa = 11.84FAAGGRR587 pKa = 11.84HH588 pKa = 4.71RR589 pKa = 11.84QVCMEE594 pKa = 4.53LARR597 pKa = 11.84RR598 pKa = 11.84YY599 pKa = 10.09AGWRR603 pKa = 11.84AGARR607 pKa = 11.84YY608 pKa = 7.32TIPDD612 pKa = 3.49GLLRR616 pKa = 11.84ALRR619 pKa = 11.84NHH621 pKa = 6.97RR622 pKa = 11.84LHH624 pKa = 6.65TYY626 pKa = 9.58VPLGRR631 pKa = 11.84IYY633 pKa = 10.41IAVHH637 pKa = 6.36HH638 pKa = 5.77MMEE641 pKa = 4.11GRR643 pKa = 11.84EE644 pKa = 3.94NQAGDD649 pKa = 3.12IRR651 pKa = 11.84SIVMDD656 pKa = 4.89RR657 pKa = 11.84NRR659 pKa = 11.84ADD661 pKa = 2.79QWRR664 pKa = 11.84RR665 pKa = 11.84IHH667 pKa = 6.94PSLPYY672 pKa = 10.55VMHH675 pKa = 6.8IEE677 pKa = 4.04FWEE680 pKa = 4.19MFVDD684 pKa = 3.83WFPDD688 pKa = 3.11VGTGGIVNMSPIFPTSLSDD707 pKa = 3.59RR708 pKa = 11.84EE709 pKa = 4.09VWVAARR715 pKa = 11.84LLL717 pKa = 3.9

Molecular weight: 81.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291ID07|A0A291ID07_9REOV Putative membrane-spanning protein (Fragment) OS=Tai Forest reovirus OX=2039230 PE=4 SV=1
LL1 pKa = 7.22RR2 pKa = 11.84RR3 pKa = 11.84PVKK6 pKa = 10.79YY7 pKa = 10.67NIFLLPTARR16 pKa = 11.84KK17 pKa = 8.64LAQKK21 pKa = 10.52LKK23 pKa = 11.03LFGKK27 pKa = 10.2LDD29 pKa = 3.89RR30 pKa = 11.84LPPEE34 pKa = 4.84DD35 pKa = 3.33WRR37 pKa = 11.84SWGALEE43 pKa = 6.28SKK45 pKa = 10.33LKK47 pKa = 10.14WLINTYY53 pKa = 9.68PSNWRR58 pKa = 11.84EE59 pKa = 3.85KK60 pKa = 10.22ISAHH64 pKa = 5.37EE65 pKa = 4.05RR66 pKa = 11.84GGMKK70 pKa = 10.23FAIQVEE76 pKa = 4.48KK77 pKa = 11.11GSDD80 pKa = 3.19VDD82 pKa = 4.11SAKK85 pKa = 9.9STIRR89 pKa = 11.84KK90 pKa = 9.28LMAQYY95 pKa = 10.79GKK97 pKa = 10.53RR98 pKa = 11.84INDD101 pKa = 3.53LQFVGVDD108 pKa = 3.61DD109 pKa = 4.86LKK111 pKa = 11.44NRR113 pKa = 11.84YY114 pKa = 8.92KK115 pKa = 10.49KK116 pKa = 10.8GKK118 pKa = 8.98IVLSVSEE125 pKa = 5.22RR126 pKa = 11.84IDD128 pKa = 3.4EE129 pKa = 4.27SAEE132 pKa = 4.29GILMKK137 pKa = 10.02WRR139 pKa = 11.84EE140 pKa = 4.01ANQDD144 pKa = 3.22QFGKK148 pKa = 11.17YY149 pKa = 7.88MGEE152 pKa = 3.78MLKK155 pKa = 10.7PKK157 pKa = 9.91MLVTDD162 pKa = 5.18FINPQAGCRR171 pKa = 11.84YY172 pKa = 8.76PNVDD176 pKa = 4.21ALSEE180 pKa = 4.58LIPEE184 pKa = 4.42DD185 pKa = 4.55SSWLFQAQAGWQMTYY200 pKa = 10.33GHH202 pKa = 7.39LRR204 pKa = 11.84PLEE207 pKa = 4.76IGMKK211 pKa = 9.84EE212 pKa = 4.02DD213 pKa = 5.19FFASSCDD220 pKa = 3.07IWCVRR225 pKa = 11.84PFSIYY230 pKa = 9.92PKK232 pKa = 10.87YY233 pKa = 10.5EE234 pKa = 3.81GLPQNFSGALAVLPTNLLTDD254 pKa = 4.27PEE256 pKa = 4.42VKK258 pKa = 10.3LQSEE262 pKa = 4.75LMSKK266 pKa = 10.82AKK268 pKa = 10.45GSSKK272 pKa = 11.16DD273 pKa = 3.48NIVFCFSYY281 pKa = 10.97LSFTFRR287 pKa = 11.84SDD289 pKa = 3.18EE290 pKa = 4.24ALSTMKK296 pKa = 10.88KK297 pKa = 10.24LGLPLAEE304 pKa = 4.14KK305 pKa = 10.09EE306 pKa = 4.53VYY308 pKa = 8.92TVLVPKK314 pKa = 10.56SVIPPLGPISGEE326 pKa = 3.81RR327 pKa = 11.84FAKK330 pKa = 10.38DD331 pKa = 3.23VLSRR335 pKa = 11.84AVGAVNTKK343 pKa = 10.49SKK345 pKa = 10.73IAMTFASGLNPDD357 pKa = 3.62VTVLCEE363 pKa = 4.16TGCGTFIDD371 pKa = 4.46GTNPRR376 pKa = 11.84MMAEE380 pKa = 4.11ILSEE384 pKa = 4.07PARR387 pKa = 11.84MIILGRR393 pKa = 11.84KK394 pKa = 8.94GGGKK398 pKa = 9.45SRR400 pKa = 11.84LGALFSEE407 pKa = 4.9LGYY410 pKa = 11.09NVIDD414 pKa = 3.55SDD416 pKa = 4.18SYY418 pKa = 11.57GRR420 pKa = 11.84LLYY423 pKa = 10.09MCGADD428 pKa = 3.35MNEE431 pKa = 3.98EE432 pKa = 4.25KK433 pKa = 10.3FLEE436 pKa = 4.71MIDD439 pKa = 3.35VYY441 pKa = 11.59LRR443 pKa = 11.84MTKK446 pKa = 9.93KK447 pKa = 10.39EE448 pKa = 3.93RR449 pKa = 11.84DD450 pKa = 3.63SVPSYY455 pKa = 11.23FEE457 pKa = 4.19VEE459 pKa = 3.74MDD461 pKa = 5.31KK462 pKa = 10.53MLQHH466 pKa = 4.75YY467 pKa = 7.03TEE469 pKa = 4.39WLGLPHH475 pKa = 6.29YY476 pKa = 10.32LSRR479 pKa = 11.84EE480 pKa = 3.67HH481 pKa = 7.44DD482 pKa = 3.48SYY484 pKa = 12.07GRR486 pKa = 11.84LDD488 pKa = 3.22RR489 pKa = 11.84ALFHH493 pKa = 6.99SFDD496 pKa = 4.32KK497 pKa = 10.8IYY499 pKa = 10.98DD500 pKa = 3.49SALRR504 pKa = 11.84KK505 pKa = 9.85VSPEE509 pKa = 3.55FMRR512 pKa = 11.84LNYY515 pKa = 8.68TKK517 pKa = 10.56RR518 pKa = 11.84LWEE521 pKa = 4.11GVGFDD526 pKa = 4.93DD527 pKa = 5.76NGIPSTGRR535 pKa = 11.84EE536 pKa = 3.52RR537 pKa = 11.84YY538 pKa = 9.51VCFCHH543 pKa = 6.44TLIEE547 pKa = 4.35QFQGMSATMFEE558 pKa = 5.6LIPSHH563 pKa = 5.25STRR566 pKa = 11.84VAISLRR572 pKa = 11.84GQGTSLNSEE581 pKa = 4.95LYY583 pKa = 10.0LHH585 pKa = 7.44DD586 pKa = 4.26YY587 pKa = 10.56YY588 pKa = 11.41VAKK591 pKa = 10.64NGNGCTKK598 pKa = 10.53VGLGWLVYY606 pKa = 9.95MLEE609 pKa = 4.16QKK611 pKa = 9.77MKK613 pKa = 10.18EE614 pKa = 3.87

Molecular weight: 69.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

7421

107

1433

742.1

84.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.926 ± 0.36

1.415 ± 0.185

6.279 ± 0.353

6.926 ± 0.361

4.299 ± 0.164

7.533 ± 0.4

2.21 ± 0.2

5.363 ± 0.333

4.609 ± 0.619

9.069 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.611 ± 0.105

3.584 ± 0.292

4.77 ± 0.302

3.059 ± 0.164

7.492 ± 0.441

5.511 ± 0.352

4.42 ± 0.381

7.775 ± 0.249

1.711 ± 0.121

3.396 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski