Saccharolobus solfataricus rod-shaped virus 1

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Rudiviridae; Hoswirudivirus; Hoswirudivirus SSRV1

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3VXB3|A0A6M3VXB3_9VIRU Uncharacterized protein OS=Saccharolobus solfataricus rod-shaped virus 1 OX=2730619 GN=SSRV1_gp01 PE=4 SV=1
MM1 pKa = 7.44NCLEE5 pKa = 4.38LLKK8 pKa = 10.75QKK10 pKa = 10.94GYY12 pKa = 10.64GLEE15 pKa = 4.7GYY17 pKa = 10.35SPDD20 pKa = 3.77TIVALEE26 pKa = 4.03EE27 pKa = 3.99ASLPEE32 pKa = 4.44DD33 pKa = 3.41EE34 pKa = 5.93CEE36 pKa = 3.83ALLWYY41 pKa = 10.74LEE43 pKa = 4.03AVYY46 pKa = 10.6GG47 pKa = 4.09

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3VXF1|A0A6M3VXF1_9VIRU Putative pyramid forming protein OS=Saccharolobus solfataricus rod-shaped virus 1 OX=2730619 GN=SSRV1_gp21 PE=4 SV=1
MM1 pKa = 7.66AKK3 pKa = 10.15GRR5 pKa = 11.84TPRR8 pKa = 11.84SFSQRR13 pKa = 11.84YY14 pKa = 8.13GKK16 pKa = 9.58WNAKK20 pKa = 7.96FTAFSNPTVASTILTNVAPIAQGNFQTNVPKK51 pKa = 9.63FTSVNEE57 pKa = 3.96QVSAVLTQYY66 pKa = 11.31GVTGPSRR73 pKa = 11.84AIYY76 pKa = 9.48QGYY79 pKa = 8.18GLKK82 pKa = 10.09VARR85 pKa = 11.84ALNRR89 pKa = 11.84IGAGPALTNMVAGLKK104 pKa = 9.93AYY106 pKa = 9.76YY107 pKa = 10.19VSAYY111 pKa = 9.29GANPEE116 pKa = 4.19ILDD119 pKa = 3.74AVTNIILGSPTGYY132 pKa = 10.74VSS134 pKa = 3.22

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

7963

47

1115

215.2

24.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.204 ± 0.406

0.804 ± 0.187

4.621 ± 0.336

6.555 ± 0.582

5.124 ± 0.356

4.634 ± 0.284

1.206 ± 0.157

9.77 ± 0.44

7.585 ± 0.666

9.77 ± 0.672

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.205

6.292 ± 0.579

3.767 ± 0.29

4.646 ± 0.434

3.202 ± 0.36

5.902 ± 0.508

4.847 ± 0.374

6.204 ± 0.335

0.867 ± 0.117

6.015 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski