Plasmodium falciparum (isolate 3D7)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Laverania); Plasmodium falciparum

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8I2W4|Q8I2W4_PLAF7 Gamma-ECS OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF3D7_0918900 PE=3 SV=1
MM1 pKa = 8.65LEE3 pKa = 3.94EE4 pKa = 4.27RR5 pKa = 11.84NEE7 pKa = 3.97NNEE10 pKa = 3.93NNEE13 pKa = 4.01DD14 pKa = 3.92NEE16 pKa = 4.49NNGYY20 pKa = 8.4NQNNEE25 pKa = 3.51YY26 pKa = 10.56DD27 pKa = 3.53QNNEE31 pKa = 3.35YY32 pKa = 10.64DD33 pKa = 3.51QNNEE37 pKa = 3.35YY38 pKa = 10.64DD39 pKa = 3.51QNNEE43 pKa = 3.35YY44 pKa = 10.64DD45 pKa = 3.51QNNEE49 pKa = 3.37YY50 pKa = 10.72DD51 pKa = 3.43QNNDD55 pKa = 3.01YY56 pKa = 10.88NQNNDD61 pKa = 3.17YY62 pKa = 10.78NQNNDD67 pKa = 3.17YY68 pKa = 10.78NQNNDD73 pKa = 3.17YY74 pKa = 10.78NQNNDD79 pKa = 3.17YY80 pKa = 10.78NQNNDD85 pKa = 3.06YY86 pKa = 10.96NLNSGYY92 pKa = 10.55NLNSGYY98 pKa = 10.59NLNSGYY104 pKa = 10.36NLNSGYY110 pKa = 10.45SLNSGYY116 pKa = 10.98SLNNVYY122 pKa = 10.32NQYY125 pKa = 11.36SNNIEE130 pKa = 3.87NSGNILNNGFFGSSTNNNNRR150 pKa = 11.84SILGGVRR157 pKa = 11.84EE158 pKa = 4.69PIQLEE163 pKa = 3.75ARR165 pKa = 11.84GFFINGRR172 pKa = 11.84SNNVNRR178 pKa = 11.84ILVNVDD184 pKa = 3.25HH185 pKa = 7.1NNVPINSLNGTNHH198 pKa = 6.96ILGIPNNINMDD209 pKa = 4.0SLIQTILLASSLIINTSARR228 pKa = 11.84AVATSNRR235 pKa = 11.84NTRR238 pKa = 11.84NSNALTSDD246 pKa = 3.54GEE248 pKa = 4.28EE249 pKa = 4.05TMEE252 pKa = 4.89EE253 pKa = 4.03IVDD256 pKa = 4.02DD257 pKa = 4.22GMEE260 pKa = 4.32EE261 pKa = 3.7IDD263 pKa = 4.67LNEE266 pKa = 4.22NSDD269 pKa = 4.44FIHH272 pKa = 7.4DD273 pKa = 4.13GNPLGEE279 pKa = 4.28YY280 pKa = 10.96SNMEE284 pKa = 4.0YY285 pKa = 10.9EE286 pKa = 4.48LLNEE290 pKa = 4.48VSASTDD296 pKa = 3.38FSDD299 pKa = 3.5YY300 pKa = 11.01HH301 pKa = 7.75SVVDD305 pKa = 3.89EE306 pKa = 4.68DD307 pKa = 5.63LINFNDD313 pKa = 3.99DD314 pKa = 3.11VTLEE318 pKa = 4.2TQSMIAHH325 pKa = 6.92GGSLSEE331 pKa = 4.27IEE333 pKa = 4.17EE334 pKa = 4.39TGDD337 pKa = 3.66LSSDD341 pKa = 3.16VDD343 pKa = 3.81RR344 pKa = 11.84LHH346 pKa = 8.1SSIEE350 pKa = 3.86TTPRR354 pKa = 11.84DD355 pKa = 3.48DD356 pKa = 3.26VVYY359 pKa = 10.51IRR361 pKa = 11.84GDD363 pKa = 3.09IVLGEE368 pKa = 4.23DD369 pKa = 4.23EE370 pKa = 5.22IIIEE374 pKa = 4.18NGEE377 pKa = 4.15IILNNIDD384 pKa = 4.03EE385 pKa = 4.79LFEE388 pKa = 4.94DD389 pKa = 4.13VPEE392 pKa = 4.08MLEE395 pKa = 4.01NANLLEE401 pKa = 4.49DD402 pKa = 3.8RR403 pKa = 11.84EE404 pKa = 4.66HH405 pKa = 7.44VFEE408 pKa = 4.42YY409 pKa = 10.54NISVANEE416 pKa = 3.48NTTNQNSYY424 pKa = 10.71NFNAPQDD431 pKa = 4.02NTLTNYY437 pKa = 10.32NISRR441 pKa = 11.84FLQRR445 pKa = 11.84YY446 pKa = 6.69GTTTPNTQHH455 pKa = 6.75NISIHH460 pKa = 6.53RR461 pKa = 11.84DD462 pKa = 3.18GHH464 pKa = 5.2SLRR467 pKa = 11.84SDD469 pKa = 3.19TNIYY473 pKa = 10.86DD474 pKa = 4.61RR475 pKa = 11.84INGPSIRR482 pKa = 11.84EE483 pKa = 3.67NSLYY487 pKa = 10.28PGSFEE492 pKa = 3.77MAQYY496 pKa = 11.03ADD498 pKa = 4.72RR499 pKa = 11.84YY500 pKa = 10.15QMGHH504 pKa = 6.64TIAEE508 pKa = 4.43GFLYY512 pKa = 10.73

Molecular weight: 58.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7NTP5|K7NTP5_PLAF7 Heat shock protein 70 OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF3D7_0831700 PE=1 SV=1
MM1 pKa = 7.28AHH3 pKa = 6.31GASRR7 pKa = 11.84YY8 pKa = 8.75KK9 pKa = 10.48KK10 pKa = 10.3SRR12 pKa = 11.84AKK14 pKa = 9.88MRR16 pKa = 11.84WKK18 pKa = 9.16WKK20 pKa = 9.29KK21 pKa = 9.72KK22 pKa = 6.67RR23 pKa = 11.84TRR25 pKa = 11.84RR26 pKa = 11.84LQKK29 pKa = 9.69KK30 pKa = 7.67RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 8.24MRR35 pKa = 11.84QRR37 pKa = 11.84SRR39 pKa = 3.22

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5383

5

5388

4118803

29

10287

764.4

90.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.957 ± 0.023

1.764 ± 0.015

6.464 ± 0.034

7.131 ± 0.083

4.381 ± 0.025

2.818 ± 0.029

2.413 ± 0.015

9.276 ± 0.044

11.745 ± 0.047

7.615 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.015

14.43 ± 0.107

1.976 ± 0.025

2.764 ± 0.018

2.633 ± 0.016

6.374 ± 0.03

4.07 ± 0.023

3.797 ± 0.052

0.491 ± 0.007

5.71 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski