Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Merbecovirus

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A3EXD1|NS3A_BCHK5 Non-structural protein 3a OS=Bat coronavirus HKU5 OX=694008 GN=3a PE=3 SV=1
MM1 pKa = 7.16IRR3 pKa = 11.84SVLVLMCSLTFIGNLTRR20 pKa = 11.84GQSVDD25 pKa = 3.09MGHH28 pKa = 6.56NGTGSCLDD36 pKa = 3.67SQVQPDD42 pKa = 4.15YY43 pKa = 11.05FEE45 pKa = 5.36SVHH48 pKa = 5.43TTWPMPIDD56 pKa = 3.44TSKK59 pKa = 11.52AEE61 pKa = 4.0GVIYY65 pKa = 10.04PNGKK69 pKa = 9.38SYY71 pKa = 11.79SNITLTYY78 pKa = 8.48TGLYY82 pKa = 9.29PKK84 pKa = 10.87ANDD87 pKa = 3.99LGKK90 pKa = 10.44QYY92 pKa = 10.84LFSDD96 pKa = 4.14GHH98 pKa = 5.51SAPGRR103 pKa = 11.84LNNLFVSNYY112 pKa = 8.4SSQVEE117 pKa = 4.3SFDD120 pKa = 3.77DD121 pKa = 4.42GFVVRR126 pKa = 11.84IGAAANKK133 pKa = 9.28TGTTVISQSTFKK145 pKa = 10.09PIKK148 pKa = 9.78KK149 pKa = 9.62IYY151 pKa = 8.89PAFLLGHH158 pKa = 5.68SVGNYY163 pKa = 7.01TPSNRR168 pKa = 11.84TGRR171 pKa = 11.84YY172 pKa = 8.95LNHH175 pKa = 6.74TLVILPDD182 pKa = 3.55GCGTILHH189 pKa = 7.04AFYY192 pKa = 10.81CVLHH196 pKa = 6.74PRR198 pKa = 11.84TQQNCAGEE206 pKa = 4.56TNFKK210 pKa = 10.74SLSLWDD216 pKa = 4.4TPASDD221 pKa = 4.53CVSGSYY227 pKa = 9.41NQEE230 pKa = 3.54ATLGAFKK237 pKa = 10.95VYY239 pKa = 10.44FDD241 pKa = 6.08LINCTFRR248 pKa = 11.84YY249 pKa = 10.19NYY251 pKa = 9.77TITEE255 pKa = 4.22DD256 pKa = 4.48EE257 pKa = 3.97NAEE260 pKa = 4.14WFGITQDD267 pKa = 3.47TQGVHH272 pKa = 6.7LYY274 pKa = 10.45SSRR277 pKa = 11.84KK278 pKa = 9.46EE279 pKa = 3.81NVFRR283 pKa = 11.84NNMFHH288 pKa = 7.46FATLPVYY295 pKa = 10.33QKK297 pKa = 10.09ILYY300 pKa = 7.25YY301 pKa = 9.63TVIPRR306 pKa = 11.84SIRR309 pKa = 11.84SPFNDD314 pKa = 2.95RR315 pKa = 11.84KK316 pKa = 10.37AWAAFYY322 pKa = 9.99IYY324 pKa = 10.33KK325 pKa = 10.23LHH327 pKa = 7.02PLTYY331 pKa = 10.54LLNFDD336 pKa = 3.62VEE338 pKa = 4.81GYY340 pKa = 7.31ITKK343 pKa = 10.51AVDD346 pKa = 3.43CGYY349 pKa = 11.23DD350 pKa = 4.06DD351 pKa = 5.81LAQLQCSYY359 pKa = 11.37EE360 pKa = 4.24SFEE363 pKa = 4.45VEE365 pKa = 3.78TGVYY369 pKa = 9.84SVSSFEE375 pKa = 3.78ASPRR379 pKa = 11.84GEE381 pKa = 4.58FIEE384 pKa = 4.21QATTQEE390 pKa = 4.29CDD392 pKa = 3.74FTPMLTGTPPPIYY405 pKa = 9.95NFKK408 pKa = 10.75RR409 pKa = 11.84LVFTNCNYY417 pKa = 10.82NLTKK421 pKa = 10.58LLSLFQVSEE430 pKa = 4.71FSCHH434 pKa = 4.9QVSPSSLATGCYY446 pKa = 9.95SSLTVDD452 pKa = 3.43YY453 pKa = 9.87FAYY456 pKa = 8.82STDD459 pKa = 3.07MSSYY463 pKa = 9.68LQPGSAGAIVQFNYY477 pKa = 10.33KK478 pKa = 9.78QDD480 pKa = 3.66FSNPTCRR487 pKa = 11.84VLATVPQNLTTITKK501 pKa = 8.73PSNYY505 pKa = 10.1AYY507 pKa = 9.51LTEE510 pKa = 4.67CYY512 pKa = 7.89KK513 pKa = 10.6TSAYY517 pKa = 9.25GKK519 pKa = 9.32NYY521 pKa = 10.18LYY523 pKa = 10.26NAPGAYY529 pKa = 8.3TPCLSLASRR538 pKa = 11.84GFSTKK543 pKa = 10.14YY544 pKa = 9.57QSHH547 pKa = 6.82SDD549 pKa = 3.76GEE551 pKa = 4.52LTTTGYY557 pKa = 10.29IYY559 pKa = 10.33PVTGNLQMAFIISVQYY575 pKa = 9.07GTDD578 pKa = 3.55TNSVCPMQALRR589 pKa = 11.84NDD591 pKa = 3.48TSIEE595 pKa = 4.13DD596 pKa = 3.73KK597 pKa = 11.26LDD599 pKa = 3.35VCVEE603 pKa = 3.97YY604 pKa = 10.66SLHH607 pKa = 6.69GITGRR612 pKa = 11.84GVFHH616 pKa = 7.07NCTSVGLRR624 pKa = 11.84NQRR627 pKa = 11.84FVYY630 pKa = 9.22DD631 pKa = 3.57TFDD634 pKa = 3.52NLVGYY639 pKa = 10.57HH640 pKa = 6.66SDD642 pKa = 3.15NGNYY646 pKa = 9.55YY647 pKa = 9.96CVRR650 pKa = 11.84PCVSVPVSVIYY661 pKa = 10.76DD662 pKa = 3.52KK663 pKa = 11.4ASNSHH668 pKa = 5.69ATLFGSVACSHH679 pKa = 5.84VTTMMSQFSRR689 pKa = 11.84MTKK692 pKa = 9.37TNLLARR698 pKa = 11.84TTPGPLQTTVGCAMGFINSSMVVDD722 pKa = 4.66EE723 pKa = 4.72CQLPLGQSLCAIPPTTSSRR742 pKa = 11.84VRR744 pKa = 11.84RR745 pKa = 11.84ATSGASDD752 pKa = 3.62VFQIATLNFTSPLTLAPINSTGFVVAVPTNFTFGVTQEE790 pKa = 4.56FIEE793 pKa = 4.32TTIQKK798 pKa = 8.34ITVDD802 pKa = 3.89CKK804 pKa = 10.81QYY806 pKa = 10.53VCNGFKK812 pKa = 10.45KK813 pKa = 10.92CEE815 pKa = 4.15DD816 pKa = 3.47LLKK819 pKa = 10.63EE820 pKa = 4.03YY821 pKa = 10.97GQFCSKK827 pKa = 10.24INQALHH833 pKa = 5.92GANLRR838 pKa = 11.84QDD840 pKa = 3.5EE841 pKa = 4.74SIANLFSSIKK851 pKa = 8.36TQNTQPLQAGLNGDD865 pKa = 4.19FNLTMLQIPQVTTGEE880 pKa = 3.87RR881 pKa = 11.84KK882 pKa = 9.32YY883 pKa = 10.94RR884 pKa = 11.84STIEE888 pKa = 4.38DD889 pKa = 3.78LLFNKK894 pKa = 7.82VTIADD899 pKa = 3.7PGYY902 pKa = 7.86MQGYY906 pKa = 8.62DD907 pKa = 2.97EE908 pKa = 5.92CMQQGPQSARR918 pKa = 11.84DD919 pKa = 4.21LICAQYY925 pKa = 9.2VAGYY929 pKa = 9.86KK930 pKa = 9.71VLPPLYY936 pKa = 9.9DD937 pKa = 3.96PYY939 pKa = 10.78MEE941 pKa = 4.81AAYY944 pKa = 9.18TSSLLGSIAGASWTAGLSSFAAIPFAQSIFYY975 pKa = 10.26RR976 pKa = 11.84LNGVGITQQVLSEE989 pKa = 4.05NQKK992 pKa = 10.46IIANKK997 pKa = 9.42FNQALGAMQTGFTTTNLAFNKK1018 pKa = 9.95VQDD1021 pKa = 3.97AVNANAMALSKK1032 pKa = 10.7LAAEE1036 pKa = 4.98LSNTFGAISSSISDD1050 pKa = 3.1ILARR1054 pKa = 11.84LDD1056 pKa = 3.62TVEE1059 pKa = 5.08QEE1061 pKa = 4.13AQIDD1065 pKa = 3.71RR1066 pKa = 11.84LINGRR1071 pKa = 11.84LTSLNAFVAQQLVRR1085 pKa = 11.84TEE1087 pKa = 3.65AAARR1091 pKa = 11.84SAQLAQDD1098 pKa = 3.82KK1099 pKa = 11.07VNEE1102 pKa = 4.39CVKK1105 pKa = 10.74SQSKK1109 pKa = 10.87RR1110 pKa = 11.84NGFCGTGTHH1119 pKa = 5.85IVSFAINAPNGLYY1132 pKa = 10.03FFHH1135 pKa = 7.75VGYY1138 pKa = 10.58QPTSHH1143 pKa = 6.33VNATAAYY1150 pKa = 8.36GLCNTEE1156 pKa = 4.45NPQKK1160 pKa = 10.6CIAPIDD1166 pKa = 4.22GYY1168 pKa = 11.21FVLNQTTSTVADD1180 pKa = 4.45SDD1182 pKa = 3.99QQWYY1186 pKa = 7.3YY1187 pKa = 9.08TGSSFFHH1194 pKa = 6.96PEE1196 pKa = 4.31PITEE1200 pKa = 4.24ANSKK1204 pKa = 8.77YY1205 pKa = 11.08VSMDD1209 pKa = 3.11VKK1211 pKa = 11.03FEE1213 pKa = 4.13NLTNRR1218 pKa = 11.84LPPPLLSNSTDD1229 pKa = 3.39LDD1231 pKa = 3.81FKK1233 pKa = 11.54EE1234 pKa = 4.12EE1235 pKa = 4.03LEE1237 pKa = 4.22EE1238 pKa = 4.02FFKK1241 pKa = 11.25NVSSQGPNFQEE1252 pKa = 3.42ISKK1255 pKa = 10.78INTTLLNLNTEE1266 pKa = 4.26LMVLSEE1272 pKa = 4.38VVKK1275 pKa = 10.44QLNEE1279 pKa = 3.95SYY1281 pKa = 10.82IDD1283 pKa = 3.87LKK1285 pKa = 11.29EE1286 pKa = 4.03LGNYY1290 pKa = 7.36TFYY1293 pKa = 11.09QKK1295 pKa = 10.17WPWYY1299 pKa = 8.52IWLGFIAGLVALALCVFFILCCTGCGTSCLGKK1331 pKa = 10.25LKK1333 pKa = 10.69CNRR1336 pKa = 11.84CCDD1339 pKa = 3.46SYY1341 pKa = 12.06DD1342 pKa = 3.43EE1343 pKa = 4.74YY1344 pKa = 10.97EE1345 pKa = 4.0VEE1347 pKa = 4.86KK1348 pKa = 10.84IHH1350 pKa = 6.28VHH1352 pKa = 5.56

Molecular weight: 149.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C6T5|R1A_BCHK5 Replicase polyprotein 1a OS=Bat coronavirus HKU5 OX=694008 GN=1a PE=3 SV=1
MM1 pKa = 7.4ATPAPPRR8 pKa = 11.84AVVFANDD15 pKa = 3.47NEE17 pKa = 4.68TPTNSQRR24 pKa = 11.84SGRR27 pKa = 11.84PRR29 pKa = 11.84TKK31 pKa = 10.13PRR33 pKa = 11.84PAPNTTVSWFTGLTQHH49 pKa = 6.72GKK51 pKa = 9.68QPLAFPPGQGVPLNANSTPAQNAGYY76 pKa = 9.33WRR78 pKa = 11.84RR79 pKa = 11.84QDD81 pKa = 3.96RR82 pKa = 11.84KK83 pKa = 10.03INTGNGTKK91 pKa = 10.09PLAPRR96 pKa = 11.84WYY98 pKa = 9.82FYY100 pKa = 10.45YY101 pKa = 10.42TGTGPEE107 pKa = 3.84ANLPFRR113 pKa = 11.84SVKK116 pKa = 10.66DD117 pKa = 3.63GIIWVHH123 pKa = 5.87EE124 pKa = 4.29NGATDD129 pKa = 3.49APSVFGTRR137 pKa = 11.84NPANDD142 pKa = 3.9PAIVTQFAPGTTLPKK157 pKa = 10.23NFHH160 pKa = 6.56IEE162 pKa = 4.18GTGGNSQSSSRR173 pKa = 11.84ASSRR177 pKa = 11.84SSSRR181 pKa = 11.84SSSRR185 pKa = 11.84NGRR188 pKa = 11.84SNNSSRR194 pKa = 11.84NASPAPHH201 pKa = 6.41GVGDD205 pKa = 3.85VVGAGTLSVLLDD217 pKa = 3.75LQKK220 pKa = 11.11RR221 pKa = 11.84LADD224 pKa = 3.87LEE226 pKa = 4.35AGKK229 pKa = 10.82GNKK232 pKa = 7.63QPKK235 pKa = 10.13VITKK239 pKa = 10.13KK240 pKa = 10.37DD241 pKa = 3.21AQAAKK246 pKa = 10.25QKK248 pKa = 9.14MRR250 pKa = 11.84HH251 pKa = 5.5KK252 pKa = 10.47RR253 pKa = 11.84VATKK257 pKa = 10.47GYY259 pKa = 10.6NVVQAFGMRR268 pKa = 11.84GPGPLQSNFGDD279 pKa = 3.44MQYY282 pKa = 11.39NKK284 pKa = 10.42LGTEE288 pKa = 4.21DD289 pKa = 4.05PRR291 pKa = 11.84WPQIAEE297 pKa = 4.2LAPSASAFMSTSQFKK312 pKa = 8.73VTHH315 pKa = 5.74QSNDD319 pKa = 3.34EE320 pKa = 4.17NGEE323 pKa = 3.87PVYY326 pKa = 10.52FLSYY330 pKa = 10.63SGAIKK335 pKa = 10.63LDD337 pKa = 3.71PKK339 pKa = 10.57NPNYY343 pKa = 9.8NKK345 pKa = 9.83WMEE348 pKa = 4.15ILDD351 pKa = 4.08ANIDD355 pKa = 3.74AYY357 pKa = 10.21KK358 pKa = 10.59SFPKK362 pKa = 10.11KK363 pKa = 9.37EE364 pKa = 4.04RR365 pKa = 11.84KK366 pKa = 9.04QKK368 pKa = 10.65PSGDD372 pKa = 3.97DD373 pKa = 3.06AATAPATSQMEE384 pKa = 4.7DD385 pKa = 3.62VPEE388 pKa = 4.56LPPKK392 pKa = 7.94QQRR395 pKa = 11.84KK396 pKa = 8.62KK397 pKa = 10.5RR398 pKa = 11.84VVQGSIPQRR407 pKa = 11.84SAGVPSFEE415 pKa = 5.04DD416 pKa = 3.28VVDD419 pKa = 5.41AIFPDD424 pKa = 4.2SEE426 pKa = 4.17AA427 pKa = 4.08

Molecular weight: 46.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

14463

82

7182

1446.3

160.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.585 ± 0.214

3.049 ± 0.264

5.352 ± 0.26

4.066 ± 0.135

4.653 ± 0.169

5.905 ± 0.177

2.026 ± 0.105

4.556 ± 0.157

5.317 ± 0.27

9.279 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.109

5.22 ± 0.194

4.107 ± 0.504

3.423 ± 0.389

3.699 ± 0.322

7.488 ± 0.27

6.873 ± 0.295

9.196 ± 0.585

1.203 ± 0.097

4.826 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski