Halogranum gelatinilyticum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halogranum

Average proteome isoelectric point is 4.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3752 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9TGR2|A0A1G9TGR2_9EURY Uncharacterized protein OS=Halogranum gelatinilyticum OX=660521 GN=SAMN04487949_1768 PE=4 SV=1
MM1 pKa = 6.93TQDD4 pKa = 3.46GTPDD8 pKa = 3.17LNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84FLQATGAVGLAGALGGVASATPGRR37 pKa = 11.84SPGPKK42 pKa = 8.82EE43 pKa = 4.72DD44 pKa = 4.33EE45 pKa = 4.17ILVGVAQGDD54 pKa = 3.95DD55 pKa = 3.63SPADD59 pKa = 3.66VVKK62 pKa = 10.74KK63 pKa = 10.36AVPGKK68 pKa = 10.58ARR70 pKa = 11.84IVHH73 pKa = 5.44EE74 pKa = 4.7NKK76 pKa = 8.83TLSYY80 pKa = 10.49VAVKK84 pKa = 10.25FPSQAADD91 pKa = 3.24VARR94 pKa = 11.84EE95 pKa = 3.92NFIEE99 pKa = 4.7AVTKK103 pKa = 10.47NDD105 pKa = 3.13RR106 pKa = 11.84VKK108 pKa = 10.83YY109 pKa = 9.58AEE111 pKa = 4.2KK112 pKa = 10.57NATLSTSLSPNDD124 pKa = 3.57PRR126 pKa = 11.84YY127 pKa = 10.03GDD129 pKa = 3.3QYY131 pKa = 11.1APQMVNADD139 pKa = 4.21TAWDD143 pKa = 3.85TTLGSSSVTIAVVDD157 pKa = 3.4TGVQYY162 pKa = 10.47DD163 pKa = 4.0HH164 pKa = 7.89PDD166 pKa = 3.15LAGSFGSNPGYY177 pKa = 10.95DD178 pKa = 3.63FVDD181 pKa = 4.6DD182 pKa = 5.92DD183 pKa = 4.79GDD185 pKa = 4.03PYY187 pKa = 11.25PDD189 pKa = 3.39VLADD193 pKa = 4.19EE194 pKa = 4.48YY195 pKa = 11.08HH196 pKa = 5.62GTHH199 pKa = 5.66VAGIAGGVTDD209 pKa = 4.69NGTGTSGISNSTLISGRR226 pKa = 11.84ALDD229 pKa = 3.64EE230 pKa = 4.35SGRR233 pKa = 11.84GSTADD238 pKa = 2.99IADD241 pKa = 3.79AVEE244 pKa = 3.79WAADD248 pKa = 3.45QGADD252 pKa = 4.73VINLSLGGGGYY263 pKa = 9.07TSTMKK268 pKa = 10.69NAVSYY273 pKa = 8.31ATNNGALVVAAAGNNGTSSVSYY295 pKa = 8.65PAAYY299 pKa = 9.87SEE301 pKa = 4.4CLAVSSLDD309 pKa = 3.55PDD311 pKa = 4.01GSLASYY317 pKa = 9.41SQYY320 pKa = 11.02GSSIEE325 pKa = 3.94LAAPGSNVLATLPTDD340 pKa = 4.4DD341 pKa = 4.44YY342 pKa = 11.7GEE344 pKa = 4.35LSGTSMATPVVAGVAGLTLAQWDD367 pKa = 3.91VDD369 pKa = 3.72NVTLRR374 pKa = 11.84DD375 pKa = 3.81HH376 pKa = 7.11LKK378 pKa = 8.74NTAVDD383 pKa = 3.33IGLSSSQQGSGRR395 pKa = 11.84VDD397 pKa = 2.82AANAVEE403 pKa = 4.18TDD405 pKa = 3.59PANGGGGDD413 pKa = 4.04GGDD416 pKa = 3.92GGDD419 pKa = 4.46DD420 pKa = 3.76GGSGGSTSASVTDD433 pKa = 3.99SLTNSADD440 pKa = 4.01SKK442 pKa = 9.22CWTYY446 pKa = 11.48AFEE449 pKa = 4.3YY450 pKa = 10.05SNPSTVVISLDD461 pKa = 3.48GPSYY465 pKa = 11.73ADD467 pKa = 3.37FDD469 pKa = 5.07LYY471 pKa = 11.57ANDD474 pKa = 4.17GTGTCPSTSSYY485 pKa = 11.27DD486 pKa = 3.55YY487 pKa = 10.99RR488 pKa = 11.84SWTTTSQEE496 pKa = 4.7SITIDD501 pKa = 4.02NPDD504 pKa = 3.15TSADD508 pKa = 3.55LSILVDD514 pKa = 3.83SYY516 pKa = 11.69SGSGSYY522 pKa = 8.54TLEE525 pKa = 3.68IVEE528 pKa = 4.42YY529 pKa = 9.92EE530 pKa = 4.0

Molecular weight: 54.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9ZGJ2|A0A1G9ZGJ2_9EURY Thiamine transport system substrate-binding protein OS=Halogranum gelatinilyticum OX=660521 GN=SAMN04487949_3629 PE=4 SV=1
MM1 pKa = 7.52SPLGAAPLLAPEE13 pKa = 5.43KK14 pKa = 10.34IPSRR18 pKa = 11.84SLRR21 pKa = 11.84SLPTASPPSILVGFWVEE38 pKa = 3.92HH39 pKa = 5.91PRR41 pKa = 11.84FSVEE45 pKa = 3.19KK46 pKa = 10.49RR47 pKa = 11.84MTLRR51 pKa = 11.84CSTHH55 pKa = 5.38FLEE58 pKa = 5.13MVNVPPKK65 pKa = 10.32FRR67 pKa = 11.84IGGTPRR73 pKa = 11.84SLAILWLEE81 pKa = 4.13ANRR84 pKa = 11.84KK85 pKa = 9.02NGWNTPCFTVCRR97 pKa = 11.84YY98 pKa = 9.22PISLPSLPPLIQYY111 pKa = 9.68HH112 pKa = 6.52DD113 pKa = 3.93PEE115 pKa = 4.53KK116 pKa = 10.92NRR118 pKa = 11.84YY119 pKa = 5.4RR120 pKa = 11.84TQRR123 pKa = 11.84KK124 pKa = 8.83RR125 pKa = 11.84EE126 pKa = 4.05QVPHH130 pKa = 5.64QAVADD135 pKa = 4.71RR136 pKa = 11.84SQIQCDD142 pKa = 3.55FRR144 pKa = 11.84AFVRR148 pKa = 11.84TNSGSQGRR156 pKa = 11.84GHH158 pKa = 6.18VALPTLHH165 pKa = 6.74PRR167 pKa = 11.84ITAPLNRR174 pKa = 11.84VSSQLLPCANFLSRR188 pKa = 11.84YY189 pKa = 8.79VIYY192 pKa = 10.64KK193 pKa = 10.22RR194 pKa = 11.84ALLRR198 pKa = 11.84NTGDD202 pKa = 3.3SDD204 pKa = 3.8RR205 pKa = 11.84QTSEE209 pKa = 4.02LALEE213 pKa = 4.34TTLLITPIITSRR225 pKa = 11.84LL226 pKa = 3.49

Molecular weight: 25.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3752

0

3752

1098223

39

1990

292.7

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.67 ± 0.058

0.682 ± 0.013

8.315 ± 0.048

8.046 ± 0.052

3.449 ± 0.026

8.567 ± 0.039

1.983 ± 0.021

3.644 ± 0.034

2.057 ± 0.03

9.226 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.753 ± 0.019

2.425 ± 0.025

4.561 ± 0.026

2.641 ± 0.024

6.334 ± 0.033

5.624 ± 0.032

6.605 ± 0.028

9.541 ± 0.042

1.155 ± 0.016

2.722 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski