Flavobacterium sp. 123

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495SLF6|A0A495SLF6_9FLAO Acetyl-CoA acyltransferase OS=Flavobacterium sp. 123 OX=2135627 GN=C8C88_2168 PE=3 SV=1
MM1 pKa = 7.56KK2 pKa = 10.42LKK4 pKa = 10.74NYY6 pKa = 10.18LPNQLIKK13 pKa = 10.7CNQEE17 pKa = 3.56STSYY21 pKa = 10.09SKK23 pKa = 11.01KK24 pKa = 10.05VFLFFAALFLTVASSYY40 pKa = 11.23SLEE43 pKa = 4.1AQNVSLTFSKK53 pKa = 10.84GYY55 pKa = 10.56LGTQGSNTNQANSINNLSNIGINRR79 pKa = 11.84ISFSQAYY86 pKa = 9.36AGAFGGTQGNDD97 pKa = 2.82LSGNLTFYY105 pKa = 10.71LANGTSFSLTGALNWRR121 pKa = 11.84EE122 pKa = 4.12TSGSTVEE129 pKa = 3.95VFGFIFDD136 pKa = 3.62SGQNHH141 pKa = 7.35TITYY145 pKa = 10.77GLGQTYY151 pKa = 10.54NIVGGSTPNTSTTLGLKK168 pKa = 10.03AYY170 pKa = 9.45ASSFVFVNGEE180 pKa = 3.74NRR182 pKa = 11.84AGNAATNGLLDD193 pKa = 4.06ALNAEE198 pKa = 5.13LLSSPQPSTVTLSTANVTEE217 pKa = 4.18GQNLVYY223 pKa = 10.74NVTLSSATTVGNPQTFVFTYY243 pKa = 10.55GGTSTNGSDD252 pKa = 3.65YY253 pKa = 11.27NSTFTFSNGVIDD265 pKa = 5.01NGDD268 pKa = 3.38GTITVPGGVSSFTITASTTDD288 pKa = 3.44DD289 pKa = 3.38SSIEE293 pKa = 4.1STEE296 pKa = 3.95TLILNIGSKK305 pKa = 10.2SDD307 pKa = 3.3SGNILDD313 pKa = 4.5NDD315 pKa = 4.22SVALTASTSQTNLFCFGGSNGTASVVVSGGTAPYY349 pKa = 9.62SYY351 pKa = 10.44SWSPSGGTWATASGLTAGNYY371 pKa = 7.39TVTITDD377 pKa = 4.36DD378 pKa = 3.47NLATITKK385 pKa = 9.28NFTITEE391 pKa = 4.32PSAITASASQTNVSCNGGSNGTASVTPSGGAGSYY425 pKa = 9.0TYY427 pKa = 10.52SWSPSGGTGATASGLSAGSYY447 pKa = 8.99TVTIEE452 pKa = 4.44DD453 pKa = 4.54ANGCSATRR461 pKa = 11.84NFTITEE467 pKa = 4.26PSAIAASASQTNIACNGGATGSASVTPSGGAGSYY501 pKa = 9.0TYY503 pKa = 10.52SWSPSGGTGATASGLTAGNYY523 pKa = 7.39TVTITDD529 pKa = 4.04ANLCTATRR537 pKa = 11.84NFTITEE543 pKa = 4.3PSAITATTSQTNTSCNGSTDD563 pKa = 4.29GIASVIASGGTGSYY577 pKa = 10.04SYY579 pKa = 10.72SWSPSGGTAATATGLAAGNYY599 pKa = 6.88TCTITDD605 pKa = 4.26LNGCSIDD612 pKa = 3.67KK613 pKa = 10.63NFTIALSSSWNDD625 pKa = 3.15FDD627 pKa = 6.33CDD629 pKa = 3.75GDD631 pKa = 4.08GVTNGTEE638 pKa = 4.25VTDD641 pKa = 3.82GTNPDD646 pKa = 3.49NSCSFTASSITLNASSSWNTADD668 pKa = 4.57CDD670 pKa = 3.83GDD672 pKa = 3.79NVTNGQEE679 pKa = 4.31LLDD682 pKa = 3.99GTDD685 pKa = 3.54PNKK688 pKa = 10.31PDD690 pKa = 3.53TDD692 pKa = 3.91GDD694 pKa = 4.18GVTDD698 pKa = 4.12GKK700 pKa = 11.16EE701 pKa = 4.01KK702 pKa = 10.28TDD704 pKa = 3.48EE705 pKa = 4.09TDD707 pKa = 3.02ATDD710 pKa = 3.36PCKK713 pKa = 10.28FVFASQSVAPSSSWNTADD731 pKa = 4.57CDD733 pKa = 3.83GDD735 pKa = 3.79NVTNGQEE742 pKa = 4.27KK743 pKa = 10.89LDD745 pKa = 3.92GTDD748 pKa = 3.28SNKK751 pKa = 9.89PDD753 pKa = 3.57TDD755 pKa = 3.69GDD757 pKa = 4.18GVTDD761 pKa = 4.15GKK763 pKa = 11.02EE764 pKa = 3.86KK765 pKa = 10.41TDD767 pKa = 3.4ATDD770 pKa = 3.31ATDD773 pKa = 3.32PCKK776 pKa = 10.28FVFASQSVTPSSSWNTADD794 pKa = 5.11CDD796 pKa = 4.05GDD798 pKa = 4.11TITNGQEE805 pKa = 4.16KK806 pKa = 10.68LDD808 pKa = 3.97GTDD811 pKa = 3.21PTKK814 pKa = 10.67PDD816 pKa = 3.39TDD818 pKa = 3.89GDD820 pKa = 4.23GVTDD824 pKa = 4.64DD825 pKa = 5.05KK826 pKa = 11.61EE827 pKa = 4.31KK828 pKa = 10.65TDD830 pKa = 3.74ATDD833 pKa = 3.31ATDD836 pKa = 3.32PCKK839 pKa = 10.28FVFASQSVAPSSSWNTGDD857 pKa = 4.56CDD859 pKa = 4.26GDD861 pKa = 3.69NVTNGQEE868 pKa = 4.31KK869 pKa = 10.86LDD871 pKa = 3.9GTDD874 pKa = 3.66PNVADD879 pKa = 3.92TDD881 pKa = 4.2GDD883 pKa = 4.15GVTDD887 pKa = 4.39DD888 pKa = 4.71KK889 pKa = 11.71EE890 pKa = 4.39KK891 pKa = 10.69TDD893 pKa = 3.48VTDD896 pKa = 4.27ATDD899 pKa = 3.19ACQFILASQSVAPRR913 pKa = 11.84SSWNTADD920 pKa = 4.11CDD922 pKa = 3.74GDD924 pKa = 3.84NVSNGQEE931 pKa = 4.5KK932 pKa = 10.02IDD934 pKa = 3.74GTDD937 pKa = 3.35PLKK940 pKa = 10.77PDD942 pKa = 3.44TDD944 pKa = 4.09GDD946 pKa = 4.28GVTDD950 pKa = 3.83GTEE953 pKa = 4.41KK954 pKa = 10.18IDD956 pKa = 3.47GTNAKK961 pKa = 7.86NTCDD965 pKa = 3.67YY966 pKa = 10.94LAASQTLSPSALWNDD981 pKa = 3.76ADD983 pKa = 4.86CDD985 pKa = 3.67NDD987 pKa = 4.54GIRR990 pKa = 11.84NGDD993 pKa = 3.87DD994 pKa = 3.07NCVFTANANQTDD1006 pKa = 3.75NDD1008 pKa = 3.73NDD1010 pKa = 4.27GLGDD1014 pKa = 3.73TCDD1017 pKa = 5.63DD1018 pKa = 5.02DD1019 pKa = 6.69DD1020 pKa = 6.85DD1021 pKa = 5.98NDD1023 pKa = 5.22GILDD1027 pKa = 3.97TVDD1030 pKa = 3.44NCPLTPNSDD1039 pKa = 3.47QADD1042 pKa = 3.73RR1043 pKa = 11.84DD1044 pKa = 3.76HH1045 pKa = 7.53DD1046 pKa = 4.32GKK1048 pKa = 11.69GDD1050 pKa = 3.66VCDD1053 pKa = 4.54LGLNVSQAITPNGDD1067 pKa = 3.68GINDD1071 pKa = 2.8TWMIYY1076 pKa = 10.4NIEE1079 pKa = 4.17QYY1081 pKa = 10.21PNSTVRR1087 pKa = 11.84VFNRR1091 pKa = 11.84WGSEE1095 pKa = 2.89IFYY1098 pKa = 10.57AHH1100 pKa = 7.43NYY1102 pKa = 8.72RR1103 pKa = 11.84NDD1105 pKa = 3.27WDD1107 pKa = 3.59GHH1109 pKa = 4.92YY1110 pKa = 10.65KK1111 pKa = 10.53NKK1113 pKa = 9.95NQSLPEE1119 pKa = 3.89TSAYY1123 pKa = 10.27YY1124 pKa = 9.61YY1125 pKa = 10.72QIDD1128 pKa = 3.52IEE1130 pKa = 4.82GNGSVDD1136 pKa = 3.77LEE1138 pKa = 3.89GWIYY1142 pKa = 9.21LTKK1145 pKa = 10.84

Molecular weight: 118.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495SIM6|A0A495SIM6_9FLAO UDP-N-acetylglucosamine 2-epimerase (Hydrolysing) OS=Flavobacterium sp. 123 OX=2135627 GN=C8C88_1831 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.35KK16 pKa = 10.31RR17 pKa = 11.84KK18 pKa = 9.82KK19 pKa = 8.65RR20 pKa = 11.84ARR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.91KK27 pKa = 10.59KK28 pKa = 9.82KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2513

0

2513

879023

30

4022

349.8

39.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.589 ± 0.057

0.797 ± 0.02

5.246 ± 0.036

6.304 ± 0.058

5.326 ± 0.045

6.43 ± 0.059

1.654 ± 0.025

8.435 ± 0.048

7.969 ± 0.072

9.134 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.028

6.265 ± 0.052

3.389 ± 0.028

3.329 ± 0.026

3.039 ± 0.035

6.642 ± 0.057

6.031 ± 0.111

6.271 ± 0.045

0.991 ± 0.018

3.92 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski