Botryotinia narcissicola

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Botryotinia

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z1J621|A0A4Z1J621_9HELO Uncharacterized protein OS=Botryotinia narcissicola OX=278944 GN=BOTNAR_0016g00310 PE=4 SV=1
MM1 pKa = 7.73HH2 pKa = 7.31INILVMVAGLYY13 pKa = 8.78TAVLANYY20 pKa = 9.5PSDD23 pKa = 4.0DD24 pKa = 3.97NKK26 pKa = 11.26DD27 pKa = 3.36AVADD31 pKa = 3.88YY32 pKa = 10.36PFDD35 pKa = 4.81PNQAGVPDD43 pKa = 3.86VALDD47 pKa = 3.78ASQAAIVSGIVSSLSSLSTALDD69 pKa = 3.49IPSSLASEE77 pKa = 4.94LSNIPTSASTLGYY90 pKa = 9.99SPTGVSQLDD99 pKa = 3.79EE100 pKa = 4.18QLSGTNKK107 pKa = 9.75PYY109 pKa = 10.23WYY111 pKa = 9.36TSLSPDD117 pKa = 3.02AKK119 pKa = 10.7SYY121 pKa = 11.58VDD123 pKa = 3.74DD124 pKa = 4.41AVSIVDD130 pKa = 3.51SVYY133 pKa = 11.06SLEE136 pKa = 4.49SVLTTAAAWKK146 pKa = 5.4TTSVGVVGGTTATPVNTWEE165 pKa = 4.41STWASSTKK173 pKa = 10.02FDD175 pKa = 4.15DD176 pKa = 4.19WGSSWTSTTGAGKK189 pKa = 9.33WDD191 pKa = 3.68SSSASSTGTDD201 pKa = 2.73NWDD204 pKa = 3.56SSWASSTGVNNWEE217 pKa = 4.25STSPSSTSSEE227 pKa = 3.73AGEE230 pKa = 4.51SVFGSSTNTKK240 pKa = 10.53DD241 pKa = 2.64RR242 pKa = 11.84TAAQTTGPIEE252 pKa = 4.29VTTSNATPVMCSLSLVLVLAVAVGAICAII281 pKa = 4.1

Molecular weight: 28.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z1I8K7|A0A4Z1I8K7_9HELO Uncharacterized protein OS=Botryotinia narcissicola OX=278944 GN=BOTNAR_0192g00010 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84PMAVLKK8 pKa = 10.2VINSRR13 pKa = 11.84GRR15 pKa = 11.84PAQLVGAPGKK25 pKa = 8.96TPMTFLVQVSAKK37 pKa = 9.73RR38 pKa = 11.84PLIMAPTSSGQSGSGPIFIGKK59 pKa = 9.12

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12236

0

12236

5465297

12

6898

446.7

49.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.669 ± 0.021

1.184 ± 0.009

5.553 ± 0.017

6.567 ± 0.026

3.76 ± 0.013

6.712 ± 0.023

2.28 ± 0.01

5.535 ± 0.018

5.492 ± 0.02

8.543 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.008

4.269 ± 0.012

5.733 ± 0.019

3.858 ± 0.016

5.635 ± 0.021

8.819 ± 0.031

6.17 ± 0.018

5.75 ± 0.015

1.38 ± 0.009

2.841 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski