Cyclovirus Equ1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Horse associated cyclovirus 1

Average proteome isoelectric point is 8.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4AQY4|A0A0H4AQY4_9CIRC Cap protein OS=Cyclovirus Equ1 OX=1673638 PE=4 SV=1
MM1 pKa = 7.14AQKK4 pKa = 10.5KK5 pKa = 8.79GWCFTYY11 pKa = 10.94NNYY14 pKa = 10.43DD15 pKa = 3.63EE16 pKa = 4.34QTLNGIFNRR25 pKa = 11.84CEE27 pKa = 4.44DD28 pKa = 3.15ICKK31 pKa = 10.24FIVVGKK37 pKa = 7.23EE38 pKa = 3.58VCPTTGTKK46 pKa = 9.95HH47 pKa = 5.17LQGYY51 pKa = 8.83CLFNEE56 pKa = 4.84RR57 pKa = 11.84KK58 pKa = 9.53RR59 pKa = 11.84FASVKK64 pKa = 9.34EE65 pKa = 4.03AIGGKK70 pKa = 7.46SHH72 pKa = 7.34IEE74 pKa = 3.76PAKK77 pKa = 10.59GSPQQNYY84 pKa = 10.28DD85 pKa = 3.36YY86 pKa = 9.17CTKK89 pKa = 10.79SGDD92 pKa = 3.52YY93 pKa = 7.22EE94 pKa = 4.42TRR96 pKa = 11.84GTLPKK101 pKa = 10.45GKK103 pKa = 10.39YY104 pKa = 6.88STLYY108 pKa = 8.96MVCEE112 pKa = 4.16EE113 pKa = 4.83LKK115 pKa = 10.91SGTPLRR121 pKa = 11.84TIALNEE127 pKa = 3.79PEE129 pKa = 4.46TFVRR133 pKa = 11.84HH134 pKa = 5.14HH135 pKa = 7.13RR136 pKa = 11.84GLQRR140 pKa = 11.84FTEE143 pKa = 4.12ILGEE147 pKa = 4.15STLRR151 pKa = 11.84DD152 pKa = 3.24WKK154 pKa = 9.99TEE156 pKa = 3.87VIVYY160 pKa = 10.04IGEE163 pKa = 4.26TGTGKK168 pKa = 10.0SRR170 pKa = 11.84KK171 pKa = 9.12CAEE174 pKa = 4.41LCGSTPTYY182 pKa = 9.94YY183 pKa = 10.58KK184 pKa = 10.51PRR186 pKa = 11.84GKK188 pKa = 8.93WWDD191 pKa = 3.72GYY193 pKa = 8.98TGQPNVIIDD202 pKa = 4.79DD203 pKa = 4.42FYY205 pKa = 11.73GWIKK209 pKa = 10.7YY210 pKa = 10.25DD211 pKa = 3.43EE212 pKa = 4.66LLRR215 pKa = 11.84ITDD218 pKa = 4.48RR219 pKa = 11.84YY220 pKa = 7.5PHH222 pKa = 6.44RR223 pKa = 11.84VPVKK227 pKa = 10.29FGYY230 pKa = 8.96QQFLAKK236 pKa = 9.85TIYY239 pKa = 8.79ITSNKK244 pKa = 8.51PMKK247 pKa = 9.66EE248 pKa = 3.46WYY250 pKa = 9.05TFDD253 pKa = 3.12ISALEE258 pKa = 3.76RR259 pKa = 11.84RR260 pKa = 11.84ITNVTLFKK268 pKa = 10.72KK269 pKa = 10.83LKK271 pKa = 9.92

Molecular weight: 31.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4AQY4|A0A0H4AQY4_9CIRC Cap protein OS=Cyclovirus Equ1 OX=1673638 PE=4 SV=1
MM1 pKa = 7.63AFRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84TYY9 pKa = 10.3RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84LPRR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84YY19 pKa = 10.19VITKK23 pKa = 9.11RR24 pKa = 11.84FRR26 pKa = 11.84PRR28 pKa = 11.84YY29 pKa = 7.59QRR31 pKa = 11.84QRR33 pKa = 11.84YY34 pKa = 6.14RR35 pKa = 11.84TRR37 pKa = 11.84KK38 pKa = 8.69QLNAGDD44 pKa = 3.98KK45 pKa = 9.97YY46 pKa = 8.44FTRR49 pKa = 11.84MHH51 pKa = 6.48YY52 pKa = 10.37SSEE55 pKa = 3.7LRR57 pKa = 11.84IVANDD62 pKa = 3.36PSTYY66 pKa = 9.54SRR68 pKa = 11.84QMRR71 pKa = 11.84IYY73 pKa = 9.62MSWFSNFIDD82 pKa = 5.5FNQNWSQYY90 pKa = 8.22KK91 pKa = 6.82VHH93 pKa = 6.83KK94 pKa = 9.94IVMKK98 pKa = 7.2FTPMFNNTQVNDD110 pKa = 3.52AVGNYY115 pKa = 9.03AVAVMNTGLGFGGTDD130 pKa = 3.44EE131 pKa = 4.39NSSTIIEE138 pKa = 4.47EE139 pKa = 3.8NRR141 pKa = 11.84MSYY144 pKa = 9.43EE145 pKa = 3.7QLVAIPSTKK154 pKa = 9.22IRR156 pKa = 11.84KK157 pKa = 6.38ATKK160 pKa = 10.06AVTLVFKK167 pKa = 10.4PKK169 pKa = 9.82IANTTGVVLGEE180 pKa = 3.9SSYY183 pKa = 10.2VHH185 pKa = 6.35NNNLIYY191 pKa = 10.48HH192 pKa = 6.48PWISTLRR199 pKa = 11.84ASTNANKK206 pKa = 9.6VPQLYY211 pKa = 9.75GAYY214 pKa = 9.52FAHH217 pKa = 6.74QGTANEE223 pKa = 4.49AVPQEE228 pKa = 4.12INYY231 pKa = 8.64MVDD234 pKa = 2.51VFAYY238 pKa = 8.67VTFKK242 pKa = 10.87GYY244 pKa = 10.56RR245 pKa = 11.84LPVSSKK251 pKa = 10.29QKK253 pKa = 9.79IRR255 pKa = 11.84QLNPVPEE262 pKa = 4.94PDD264 pKa = 3.41MTDD267 pKa = 2.82MM268 pKa = 5.7

Molecular weight: 31.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

539

268

271

269.5

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.638 ± 1.215

1.67 ± 1.19

3.525 ± 0.385

5.195 ± 1.308

4.638 ± 0.151

6.308 ± 1.57

1.855 ± 0.007

5.751 ± 0.376

7.607 ± 1.432

5.566 ± 0.775

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 1.074

5.751 ± 1.485

4.824 ± 0.285

4.082 ± 0.548

7.792 ± 1.36

5.195 ± 0.818

8.534 ± 0.763

6.122 ± 1.221

1.67 ± 0.392

6.679 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski