Alternaria sp. MG1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria; unclassified Alternaria

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399GBW8|A0A399GBW8_9PLEO Uncharacterized protein OS=Alternaria sp. MG1 OX=1678846 GN=CUC08_Gglean010609 PE=4 SV=1
MM1 pKa = 7.05QAYY4 pKa = 7.52KK5 pKa = 10.72QKK7 pKa = 8.76MAEE10 pKa = 3.99MEE12 pKa = 4.15AEE14 pKa = 3.6LRR16 pKa = 11.84YY17 pKa = 10.06YY18 pKa = 9.89RR19 pKa = 11.84SKK21 pKa = 11.45YY22 pKa = 9.4PGEE25 pKa = 4.38APQQVFDD32 pKa = 4.58LATPPSDD39 pKa = 3.6TFDD42 pKa = 4.26TICPAQIPTSFPSPPEE58 pKa = 4.2SMDD61 pKa = 3.96SMDD64 pKa = 3.86SPRR67 pKa = 11.84DD68 pKa = 3.57SSCQPEE74 pKa = 4.43TPPSSFEE81 pKa = 3.83ASPEE85 pKa = 3.96FDD87 pKa = 3.17STQYY91 pKa = 10.18PAAILCDD98 pKa = 3.71LPFDD102 pKa = 4.13EE103 pKa = 5.78KK104 pKa = 11.22SVVMEE109 pKa = 4.14NFFDD113 pKa = 4.57FEE115 pKa = 4.64PFPKK119 pKa = 10.39CSPTTTAPMEE129 pKa = 4.32PAFSMVDD136 pKa = 3.31SSSGDD141 pKa = 3.35VFGPTPFDD149 pKa = 3.32HH150 pKa = 7.49SSFSNSYY157 pKa = 11.06AFLDD161 pKa = 4.14GFDD164 pKa = 4.42AKK166 pKa = 11.08FNDD169 pKa = 3.76LQSASGATSVSDD181 pKa = 3.34EE182 pKa = 4.22ALAAGQLL189 pKa = 3.68

Molecular weight: 20.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399GBR0|A0A399GBR0_9PLEO Carboxylic ester hydrolase OS=Alternaria sp. MG1 OX=1678846 GN=CUC08_Gglean009179 PE=3 SV=1
MM1 pKa = 7.28FVTSVINAATCRR13 pKa = 11.84RR14 pKa = 11.84LPDD17 pKa = 3.48TKK19 pKa = 10.28GWRR22 pKa = 11.84PASLYY27 pKa = 9.64DD28 pKa = 3.58TIRR31 pKa = 11.84GRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84DD36 pKa = 3.44IQVVFLAAKK45 pKa = 9.47VLAIRR50 pKa = 11.84YY51 pKa = 7.68MVDD54 pKa = 2.43RR55 pKa = 11.84WEE57 pKa = 4.08SWRR60 pKa = 11.84SEE62 pKa = 3.82RR63 pKa = 11.84RR64 pKa = 11.84ICQIWTVLPKK74 pKa = 10.5FGRR77 pKa = 11.84PRR79 pKa = 11.84VLYY82 pKa = 9.79VPWKK86 pKa = 9.37LCGLRR91 pKa = 11.84VIVCCVKK98 pKa = 10.14IPKK101 pKa = 9.33EE102 pKa = 3.9HH103 pKa = 5.97TRR105 pKa = 11.84SIGLEE110 pKa = 3.62CSYY113 pKa = 11.21VFKK116 pKa = 11.13AA117 pKa = 4.19

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4966

0

4966

2220217

52

5387

447.1

49.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.648 ± 0.029

1.219 ± 0.012

5.784 ± 0.023

6.245 ± 0.034

3.674 ± 0.025

6.737 ± 0.034

2.37 ± 0.016

4.863 ± 0.022

5.11 ± 0.032

8.582 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.014

3.769 ± 0.019

5.864 ± 0.04

4.114 ± 0.028

5.946 ± 0.028

8.005 ± 0.044

6.098 ± 0.025

6.037 ± 0.023

1.503 ± 0.014

2.861 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski