Amino acid dipepetide frequency for Lactobacillus phage BH1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.052AlaAla: 8.052 ± 2.245
0.644AlaCys: 0.644 ± 0.247
7.81AlaAsp: 7.81 ± 1.112
4.267AlaGlu: 4.267 ± 0.625
3.221AlaPhe: 3.221 ± 0.569
4.75AlaGly: 4.75 ± 0.626
1.288AlaHis: 1.288 ± 0.28
4.509AlaIle: 4.509 ± 0.723
7.327AlaLys: 7.327 ± 1.168
7.407AlaLeu: 7.407 ± 0.918
2.496AlaMet: 2.496 ± 0.619
4.831AlaAsn: 4.831 ± 0.358
2.093AlaPro: 2.093 ± 0.461
2.576AlaGln: 2.576 ± 0.465
2.576AlaArg: 2.576 ± 0.442
5.556AlaSer: 5.556 ± 0.903
4.67AlaThr: 4.67 ± 0.641
4.831AlaVal: 4.831 ± 0.715
0.805AlaTrp: 0.805 ± 0.314
2.496AlaTyr: 2.496 ± 0.487
0.0AlaXaa: 0.0 ± 0.0
Cys
0.242CysAla: 0.242 ± 0.145
0.081CysCys: 0.081 ± 0.077
0.242CysAsp: 0.242 ± 0.154
0.483CysGlu: 0.483 ± 0.202
0.081CysPhe: 0.081 ± 0.084
0.564CysGly: 0.564 ± 0.2
0.242CysHis: 0.242 ± 0.146
0.081CysIle: 0.081 ± 0.092
0.161CysLys: 0.161 ± 0.107
0.725CysLeu: 0.725 ± 0.224
0.161CysMet: 0.161 ± 0.113
0.242CysAsn: 0.242 ± 0.152
0.161CysPro: 0.161 ± 0.127
0.242CysGln: 0.242 ± 0.127
0.161CysArg: 0.161 ± 0.109
0.0CysSer: 0.0 ± 0.0
0.403CysThr: 0.403 ± 0.174
0.161CysVal: 0.161 ± 0.122
0.0CysTrp: 0.0 ± 0.0
0.081CysTyr: 0.081 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
4.75AspAla: 4.75 ± 0.509
0.322AspCys: 0.322 ± 0.166
5.072AspAsp: 5.072 ± 0.726
3.784AspGlu: 3.784 ± 0.532
3.221AspPhe: 3.221 ± 0.462
6.28AspGly: 6.28 ± 1.245
1.61AspHis: 1.61 ± 0.406
4.026AspIle: 4.026 ± 0.638
4.589AspLys: 4.589 ± 0.558
5.233AspLeu: 5.233 ± 0.694
1.691AspMet: 1.691 ± 0.284
3.945AspAsn: 3.945 ± 0.735
3.704AspPro: 3.704 ± 0.637
2.818AspGln: 2.818 ± 0.355
3.704AspArg: 3.704 ± 0.616
5.153AspSer: 5.153 ± 0.789
3.704AspThr: 3.704 ± 0.75
4.67AspVal: 4.67 ± 0.617
1.208AspTrp: 1.208 ± 0.259
3.382AspTyr: 3.382 ± 0.516
0.0AspXaa: 0.0 ± 0.0
Glu
4.267GluAla: 4.267 ± 0.837
0.081GluCys: 0.081 ± 0.086
3.301GluAsp: 3.301 ± 0.526
2.738GluGlu: 2.738 ± 0.476
2.093GluPhe: 2.093 ± 0.32
2.818GluGly: 2.818 ± 0.325
1.288GluHis: 1.288 ± 0.307
2.979GluIle: 2.979 ± 0.554
3.945GluLys: 3.945 ± 0.832
6.119GluLeu: 6.119 ± 0.701
1.932GluMet: 1.932 ± 0.348
2.254GluAsn: 2.254 ± 0.447
1.691GluPro: 1.691 ± 0.423
3.462GluGln: 3.462 ± 0.505
3.382GluArg: 3.382 ± 0.695
2.738GluSer: 2.738 ± 0.366
3.462GluThr: 3.462 ± 0.55
4.026GluVal: 4.026 ± 0.634
0.805GluTrp: 0.805 ± 0.271
2.013GluTyr: 2.013 ± 0.542
0.0GluXaa: 0.0 ± 0.0
Phe
3.06PheAla: 3.06 ± 0.395
0.081PheCys: 0.081 ± 0.078
2.738PheAsp: 2.738 ± 0.505
2.335PheGlu: 2.335 ± 0.581
0.886PhePhe: 0.886 ± 0.292
2.979PheGly: 2.979 ± 0.457
0.644PheHis: 0.644 ± 0.234
2.174PheIle: 2.174 ± 0.554
2.899PheLys: 2.899 ± 0.448
2.496PheLeu: 2.496 ± 0.532
1.208PheMet: 1.208 ± 0.301
1.449PheAsn: 1.449 ± 0.445
1.047PhePro: 1.047 ± 0.254
1.691PheGln: 1.691 ± 0.388
1.288PheArg: 1.288 ± 0.351
2.335PheSer: 2.335 ± 0.481
3.06PheThr: 3.06 ± 0.463
1.691PheVal: 1.691 ± 0.303
0.483PheTrp: 0.483 ± 0.221
1.047PheTyr: 1.047 ± 0.332
0.0PheXaa: 0.0 ± 0.0
Gly
4.187GlyAla: 4.187 ± 0.833
0.081GlyCys: 0.081 ± 0.092
4.911GlyAsp: 4.911 ± 0.688
3.543GlyGlu: 3.543 ± 0.473
2.576GlyPhe: 2.576 ± 0.346
3.543GlyGly: 3.543 ± 0.606
1.208GlyHis: 1.208 ± 0.273
4.589GlyIle: 4.589 ± 0.668
6.28GlyLys: 6.28 ± 0.71
4.509GlyLeu: 4.509 ± 0.602
2.899GlyMet: 2.899 ± 0.405
3.301GlyAsn: 3.301 ± 0.501
1.771GlyPro: 1.771 ± 0.55
2.013GlyGln: 2.013 ± 0.365
2.818GlyArg: 2.818 ± 0.516
3.865GlySer: 3.865 ± 0.68
4.589GlyThr: 4.589 ± 0.774
4.509GlyVal: 4.509 ± 0.581
1.288GlyTrp: 1.288 ± 0.265
3.704GlyTyr: 3.704 ± 0.651
0.0GlyXaa: 0.0 ± 0.0
His
1.691HisAla: 1.691 ± 0.288
0.161HisCys: 0.161 ± 0.104
1.288HisAsp: 1.288 ± 0.202
0.483HisGlu: 0.483 ± 0.199
0.725HisPhe: 0.725 ± 0.268
0.966HisGly: 0.966 ± 0.234
0.403HisHis: 0.403 ± 0.24
1.53HisIle: 1.53 ± 0.37
0.966HisLys: 0.966 ± 0.274
1.771HisLeu: 1.771 ± 0.381
0.403HisMet: 0.403 ± 0.187
0.644HisAsn: 0.644 ± 0.342
0.644HisPro: 0.644 ± 0.156
0.644HisGln: 0.644 ± 0.182
0.886HisArg: 0.886 ± 0.301
1.771HisSer: 1.771 ± 0.439
1.288HisThr: 1.288 ± 0.265
1.449HisVal: 1.449 ± 0.395
0.403HisTrp: 0.403 ± 0.19
0.564HisTyr: 0.564 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
3.945IleAla: 3.945 ± 0.538
0.322IleCys: 0.322 ± 0.189
5.314IleAsp: 5.314 ± 0.631
4.509IleGlu: 4.509 ± 0.516
1.127IlePhe: 1.127 ± 0.386
3.382IleGly: 3.382 ± 0.436
1.127IleHis: 1.127 ± 0.297
3.301IleIle: 3.301 ± 0.486
5.958IleLys: 5.958 ± 0.537
2.899IleLeu: 2.899 ± 0.481
1.127IleMet: 1.127 ± 0.317
3.221IleAsn: 3.221 ± 0.461
2.013IlePro: 2.013 ± 0.405
2.174IleGln: 2.174 ± 0.445
2.899IleArg: 2.899 ± 0.362
4.67IleSer: 4.67 ± 0.513
4.67IleThr: 4.67 ± 0.611
3.221IleVal: 3.221 ± 0.402
0.725IleTrp: 0.725 ± 0.246
2.013IleTyr: 2.013 ± 0.356
0.0IleXaa: 0.0 ± 0.0
Lys
6.683LysAla: 6.683 ± 0.923
0.161LysCys: 0.161 ± 0.124
5.072LysAsp: 5.072 ± 0.643
4.348LysGlu: 4.348 ± 0.736
3.221LysPhe: 3.221 ± 0.487
4.267LysGly: 4.267 ± 0.809
1.61LysHis: 1.61 ± 0.311
3.865LysIle: 3.865 ± 0.496
5.153LysLys: 5.153 ± 0.676
6.2LysLeu: 6.2 ± 0.829
1.53LysMet: 1.53 ± 0.328
3.865LysAsn: 3.865 ± 0.69
2.979LysPro: 2.979 ± 0.369
3.945LysGln: 3.945 ± 0.555
3.221LysArg: 3.221 ± 0.527
5.153LysSer: 5.153 ± 0.468
5.314LysThr: 5.314 ± 0.643
4.589LysVal: 4.589 ± 0.77
1.369LysTrp: 1.369 ± 0.31
2.738LysTyr: 2.738 ± 0.588
0.0LysXaa: 0.0 ± 0.0
Leu
7.568LeuAla: 7.568 ± 0.687
0.644LeuCys: 0.644 ± 0.241
6.522LeuAsp: 6.522 ± 0.667
3.704LeuGlu: 3.704 ± 0.457
2.576LeuPhe: 2.576 ± 0.47
5.475LeuGly: 5.475 ± 0.714
1.369LeuHis: 1.369 ± 0.285
4.992LeuIle: 4.992 ± 0.485
5.636LeuLys: 5.636 ± 0.891
4.992LeuLeu: 4.992 ± 0.549
1.852LeuMet: 1.852 ± 0.378
3.623LeuAsn: 3.623 ± 0.572
3.301LeuPro: 3.301 ± 0.369
4.106LeuGln: 4.106 ± 0.606
3.623LeuArg: 3.623 ± 0.579
5.717LeuSer: 5.717 ± 0.87
4.911LeuThr: 4.911 ± 0.616
4.67LeuVal: 4.67 ± 0.645
0.725LeuTrp: 0.725 ± 0.242
2.174LeuTyr: 2.174 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
2.174MetAla: 2.174 ± 0.407
0.081MetCys: 0.081 ± 0.075
1.208MetAsp: 1.208 ± 0.28
0.644MetGlu: 0.644 ± 0.22
1.047MetPhe: 1.047 ± 0.2
1.61MetGly: 1.61 ± 0.41
0.483MetHis: 0.483 ± 0.184
1.449MetIle: 1.449 ± 0.287
2.335MetLys: 2.335 ± 0.368
1.771MetLeu: 1.771 ± 0.283
0.725MetMet: 0.725 ± 0.282
1.771MetAsn: 1.771 ± 0.353
1.047MetPro: 1.047 ± 0.273
1.61MetGln: 1.61 ± 0.363
1.369MetArg: 1.369 ± 0.345
2.576MetSer: 2.576 ± 0.47
2.013MetThr: 2.013 ± 0.48
1.771MetVal: 1.771 ± 0.338
0.081MetTrp: 0.081 ± 0.082
1.047MetTyr: 1.047 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
4.348AsnAla: 4.348 ± 0.696
0.081AsnCys: 0.081 ± 0.074
3.784AsnAsp: 3.784 ± 0.53
3.382AsnGlu: 3.382 ± 0.478
1.208AsnPhe: 1.208 ± 0.327
4.267AsnGly: 4.267 ± 0.516
1.53AsnHis: 1.53 ± 0.344
2.818AsnIle: 2.818 ± 0.495
3.704AsnLys: 3.704 ± 0.612
4.026AsnLeu: 4.026 ± 0.532
1.932AsnMet: 1.932 ± 0.355
2.415AsnAsn: 2.415 ± 0.377
2.335AsnPro: 2.335 ± 0.53
1.932AsnGln: 1.932 ± 0.415
3.06AsnArg: 3.06 ± 0.398
2.979AsnSer: 2.979 ± 0.527
2.415AsnThr: 2.415 ± 0.44
3.301AsnVal: 3.301 ± 0.509
0.966AsnTrp: 0.966 ± 0.374
1.932AsnTyr: 1.932 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
3.382ProAla: 3.382 ± 0.51
0.0ProCys: 0.0 ± 0.0
3.14ProAsp: 3.14 ± 0.565
3.14ProGlu: 3.14 ± 0.677
1.047ProPhe: 1.047 ± 0.208
2.093ProGly: 2.093 ± 0.673
0.564ProHis: 0.564 ± 0.22
1.852ProIle: 1.852 ± 0.363
2.657ProLys: 2.657 ± 0.399
2.738ProLeu: 2.738 ± 0.5
0.725ProMet: 0.725 ± 0.314
2.496ProAsn: 2.496 ± 0.566
0.725ProPro: 0.725 ± 0.3
1.691ProGln: 1.691 ± 0.332
0.805ProArg: 0.805 ± 0.218
3.06ProSer: 3.06 ± 0.437
1.61ProThr: 1.61 ± 0.318
2.576ProVal: 2.576 ± 0.543
0.564ProTrp: 0.564 ± 0.183
1.449ProTyr: 1.449 ± 0.476
0.0ProXaa: 0.0 ± 0.0
Gln
5.153GlnAla: 5.153 ± 0.773
0.081GlnCys: 0.081 ± 0.075
1.771GlnAsp: 1.771 ± 0.361
2.576GlnGlu: 2.576 ± 0.438
1.932GlnPhe: 1.932 ± 0.442
2.657GlnGly: 2.657 ± 0.467
1.127GlnHis: 1.127 ± 0.318
3.382GlnIle: 3.382 ± 0.498
3.301GlnLys: 3.301 ± 0.409
4.267GlnLeu: 4.267 ± 0.528
1.61GlnMet: 1.61 ± 0.329
1.691GlnAsn: 1.691 ± 0.364
1.127GlnPro: 1.127 ± 0.325
2.738GlnGln: 2.738 ± 0.554
1.771GlnArg: 1.771 ± 0.373
2.496GlnSer: 2.496 ± 0.537
2.335GlnThr: 2.335 ± 0.384
2.576GlnVal: 2.576 ± 0.427
0.725GlnTrp: 0.725 ± 0.218
1.771GlnTyr: 1.771 ± 0.305
0.0GlnXaa: 0.0 ± 0.0
Arg
3.462ArgAla: 3.462 ± 0.586
0.322ArgCys: 0.322 ± 0.207
2.979ArgAsp: 2.979 ± 0.681
2.335ArgGlu: 2.335 ± 0.439
1.852ArgPhe: 1.852 ± 0.358
3.06ArgGly: 3.06 ± 0.4
1.047ArgHis: 1.047 ± 0.252
2.818ArgIle: 2.818 ± 0.465
4.187ArgLys: 4.187 ± 0.507
4.026ArgLeu: 4.026 ± 0.586
1.449ArgMet: 1.449 ± 0.306
1.449ArgAsn: 1.449 ± 0.331
0.886ArgPro: 0.886 ± 0.259
2.415ArgGln: 2.415 ± 0.406
1.852ArgArg: 1.852 ± 0.405
2.496ArgSer: 2.496 ± 0.445
2.657ArgThr: 2.657 ± 0.373
3.221ArgVal: 3.221 ± 0.475
0.564ArgTrp: 0.564 ± 0.164
1.932ArgTyr: 1.932 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
4.187SerAla: 4.187 ± 0.681
0.161SerCys: 0.161 ± 0.116
3.945SerAsp: 3.945 ± 0.798
3.221SerGlu: 3.221 ± 0.4
2.496SerPhe: 2.496 ± 0.453
5.797SerGly: 5.797 ± 0.859
0.725SerHis: 0.725 ± 0.256
4.75SerIle: 4.75 ± 0.529
4.75SerLys: 4.75 ± 0.698
5.475SerLeu: 5.475 ± 0.725
2.013SerMet: 2.013 ± 0.361
4.106SerAsn: 4.106 ± 0.579
2.013SerPro: 2.013 ± 0.494
3.221SerGln: 3.221 ± 0.444
3.543SerArg: 3.543 ± 0.4
4.589SerSer: 4.589 ± 0.608
5.153SerThr: 5.153 ± 0.803
4.67SerVal: 4.67 ± 0.7
1.047SerTrp: 1.047 ± 0.289
2.657SerTyr: 2.657 ± 0.576
0.0SerXaa: 0.0 ± 0.0
Thr
6.28ThrAla: 6.28 ± 1.275
0.161ThrCys: 0.161 ± 0.114
4.911ThrAsp: 4.911 ± 0.622
2.899ThrGlu: 2.899 ± 0.535
2.093ThrPhe: 2.093 ± 0.339
4.026ThrGly: 4.026 ± 0.427
0.805ThrHis: 0.805 ± 0.26
3.543ThrIle: 3.543 ± 0.454
3.623ThrLys: 3.623 ± 0.46
4.911ThrLeu: 4.911 ± 0.524
1.208ThrMet: 1.208 ± 0.331
4.026ThrAsn: 4.026 ± 0.695
3.623ThrPro: 3.623 ± 0.518
2.254ThrGln: 2.254 ± 0.316
2.415ThrArg: 2.415 ± 0.375
4.106ThrSer: 4.106 ± 0.559
4.267ThrThr: 4.267 ± 0.729
4.187ThrVal: 4.187 ± 0.672
0.805ThrTrp: 0.805 ± 0.312
2.576ThrTyr: 2.576 ± 0.611
0.0ThrXaa: 0.0 ± 0.0
Val
4.589ValAla: 4.589 ± 0.455
0.322ValCys: 0.322 ± 0.159
5.153ValAsp: 5.153 ± 0.842
3.462ValGlu: 3.462 ± 0.648
1.932ValPhe: 1.932 ± 0.436
4.348ValGly: 4.348 ± 0.553
0.966ValHis: 0.966 ± 0.258
3.06ValIle: 3.06 ± 0.389
3.945ValLys: 3.945 ± 0.569
4.589ValLeu: 4.589 ± 0.523
1.288ValMet: 1.288 ± 0.36
4.267ValAsn: 4.267 ± 0.465
3.06ValPro: 3.06 ± 0.653
2.738ValGln: 2.738 ± 0.47
2.899ValArg: 2.899 ± 0.424
5.556ValSer: 5.556 ± 0.526
3.301ValThr: 3.301 ± 0.603
4.428ValVal: 4.428 ± 0.858
1.127ValTrp: 1.127 ± 0.268
1.932ValTyr: 1.932 ± 0.436
0.0ValXaa: 0.0 ± 0.0
Trp
1.127TrpAla: 1.127 ± 0.426
0.322TrpCys: 0.322 ± 0.149
1.127TrpAsp: 1.127 ± 0.326
0.886TrpGlu: 0.886 ± 0.227
0.403TrpPhe: 0.403 ± 0.155
0.725TrpGly: 0.725 ± 0.205
0.242TrpHis: 0.242 ± 0.118
1.047TrpIle: 1.047 ± 0.301
1.127TrpLys: 1.127 ± 0.259
1.127TrpLeu: 1.127 ± 0.334
0.081TrpMet: 0.081 ± 0.075
0.886TrpAsn: 0.886 ± 0.49
0.242TrpPro: 0.242 ± 0.16
1.047TrpGln: 1.047 ± 0.212
1.208TrpArg: 1.208 ± 0.338
0.886TrpSer: 0.886 ± 0.285
0.805TrpThr: 0.805 ± 0.207
0.725TrpVal: 0.725 ± 0.264
0.483TrpTrp: 0.483 ± 0.223
0.564TrpTyr: 0.564 ± 0.257
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.06TyrAla: 3.06 ± 0.572
0.403TyrCys: 0.403 ± 0.2
2.576TyrAsp: 2.576 ± 0.376
2.738TyrGlu: 2.738 ± 0.61
1.852TyrPhe: 1.852 ± 0.387
2.576TyrGly: 2.576 ± 0.45
0.403TyrHis: 0.403 ± 0.166
1.852TyrIle: 1.852 ± 0.573
2.738TyrLys: 2.738 ± 0.608
2.899TyrLeu: 2.899 ± 0.535
0.403TyrMet: 0.403 ± 0.136
1.932TyrAsn: 1.932 ± 0.41
1.852TyrPro: 1.852 ± 0.356
1.771TyrGln: 1.771 ± 0.293
1.53TyrArg: 1.53 ± 0.37
2.818TyrSer: 2.818 ± 0.482
2.093TyrThr: 2.093 ± 0.445
1.691TyrVal: 1.691 ± 0.399
0.886TyrTrp: 0.886 ± 0.288
1.53TyrTyr: 1.53 ± 0.356
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12421 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski