Pseudoclavibacter sp. CFCC 13611

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Pseudoclavibacter; unclassified Pseudoclavibacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H9WSU0|A0A6H9WSU0_9MICO Pyrimidine reductase family protein OS=Pseudoclavibacter sp. CFCC 13611 OX=2615178 GN=F8O08_02820 PE=4 SV=1
MM1 pKa = 7.52TYY3 pKa = 10.59VIALPCVDD11 pKa = 3.9VKK13 pKa = 11.24DD14 pKa = 3.85RR15 pKa = 11.84ACIEE19 pKa = 4.23EE20 pKa = 4.62CPVDD24 pKa = 4.81CIYY27 pKa = 11.15EE28 pKa = 4.18GDD30 pKa = 3.56RR31 pKa = 11.84TLYY34 pKa = 9.81IHH36 pKa = 7.52PDD38 pKa = 3.07EE39 pKa = 5.5CVDD42 pKa = 4.38CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.4AIYY56 pKa = 11.22YY57 pKa = 10.15EE58 pKa = 4.88DD59 pKa = 5.11DD60 pKa = 5.08LPEE63 pKa = 4.16EE64 pKa = 3.8WSDD67 pKa = 3.7YY68 pKa = 10.69YY69 pKa = 10.9RR70 pKa = 11.84INVDD74 pKa = 4.46FFQEE78 pKa = 4.0LGSPGGAAKK87 pKa = 10.34VGPTGYY93 pKa = 10.36DD94 pKa = 3.04HH95 pKa = 7.39PAVRR99 pKa = 11.84DD100 pKa = 3.71LPPQGEE106 pKa = 4.3GHH108 pKa = 6.59

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H9WXF7|A0A6H9WXF7_9MICO Uncharacterized protein OS=Pseudoclavibacter sp. CFCC 13611 OX=2615178 GN=F8O08_05720 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1915

0

1915

666035

32

2496

347.8

37.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.44 ± 0.075

0.553 ± 0.013

6.303 ± 0.049

5.607 ± 0.054

3.073 ± 0.037

8.448 ± 0.045

2.318 ± 0.027

4.605 ± 0.041

2.374 ± 0.046

10.045 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.023

2.182 ± 0.031

5.052 ± 0.037

3.793 ± 0.036

7.166 ± 0.066

6.111 ± 0.044

6.122 ± 0.044

8.75 ± 0.048

1.387 ± 0.022

1.92 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski