Leptonychotes weddellii polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L4AB48|A0A1L4AB48_9POLY ALTO OS=Leptonychotes weddellii polyomavirus 1 OX=1925019 PE=4 SV=1
MM1 pKa = 7.46GALISVITEE10 pKa = 4.14LFEE13 pKa = 4.08LAAATGFTTEE23 pKa = 5.09AILSGEE29 pKa = 3.9AAASIEE35 pKa = 3.96ALLDD39 pKa = 3.35EE40 pKa = 4.53VAYY43 pKa = 10.77VSLVDD48 pKa = 5.4GVTDD52 pKa = 4.14LQALEE57 pKa = 4.24SFGISEE63 pKa = 4.25EE64 pKa = 4.15LFSRR68 pKa = 11.84FLDD71 pKa = 3.57IPLGASEE78 pKa = 4.47VLASLGEE85 pKa = 4.25GVKK88 pKa = 10.82GSLGEE93 pKa = 4.01LTVIALSIAAASLSPLSYY111 pKa = 10.42SQAVPDD117 pKa = 3.6MALQVWVPDD126 pKa = 3.15VDD128 pKa = 3.94YY129 pKa = 10.84LFPGVSVFARR139 pKa = 11.84YY140 pKa = 8.39AQYY143 pKa = 11.16LDD145 pKa = 3.48PAYY148 pKa = 9.64WGPDD152 pKa = 3.31LLNHH156 pKa = 6.21VSRR159 pKa = 11.84LFWQSLVSEE168 pKa = 4.68GRR170 pKa = 11.84RR171 pKa = 11.84QIGHH175 pKa = 7.75ASRR178 pKa = 11.84EE179 pKa = 4.09LATQVGGTVQEE190 pKa = 4.13SLARR194 pKa = 11.84FFEE197 pKa = 4.11NTRR200 pKa = 11.84WAISHH205 pKa = 5.5VTGDD209 pKa = 3.09WYY211 pKa = 11.12GALQRR216 pKa = 11.84YY217 pKa = 8.97YY218 pKa = 11.49GEE220 pKa = 5.09LPPLNPPQISQLRR233 pKa = 11.84RR234 pKa = 11.84RR235 pKa = 11.84IEE237 pKa = 4.1RR238 pKa = 11.84DD239 pKa = 2.79TGIDD243 pKa = 3.39VADD246 pKa = 3.66VAEE249 pKa = 4.25EE250 pKa = 4.24DD251 pKa = 3.55KK252 pKa = 11.12RR253 pKa = 11.84HH254 pKa = 5.34YY255 pKa = 10.4PSANFIQKK263 pKa = 9.82YY264 pKa = 7.95SAPGGAGQRR273 pKa = 11.84HH274 pKa = 5.43APDD277 pKa = 3.09WLLPLLLGLYY287 pKa = 10.72GDD289 pKa = 5.21LTPTWRR295 pKa = 11.84KK296 pKa = 8.38EE297 pKa = 3.9VEE299 pKa = 3.97EE300 pKa = 4.33LEE302 pKa = 4.47EE303 pKa = 5.49IEE305 pKa = 4.62TEE307 pKa = 4.62DD308 pKa = 5.08EE309 pKa = 3.8EE310 pKa = 5.42DD311 pKa = 3.8VPKK314 pKa = 10.68KK315 pKa = 9.23KK316 pKa = 9.86QRR318 pKa = 11.84RR319 pKa = 11.84ASFSGPQTSNKK330 pKa = 8.89RR331 pKa = 11.84RR332 pKa = 11.84NRR334 pKa = 11.84SVRR337 pKa = 11.84RR338 pKa = 11.84PNRR341 pKa = 11.84RR342 pKa = 11.84RR343 pKa = 3.25

Molecular weight: 38.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L4AB49|A0A1L4AB49_9POLY Minor capsid protein OS=Leptonychotes weddellii polyomavirus 1 OX=1925019 PE=3 SV=1
MM1 pKa = 7.28EE2 pKa = 5.99HH3 pKa = 6.27LTGINGGMPSIKK15 pKa = 9.51IGAVRR20 pKa = 11.84RR21 pKa = 11.84TCIVMRR27 pKa = 11.84HH28 pKa = 6.71WIPQMMRR35 pKa = 11.84VHH37 pKa = 5.96KK38 pKa = 6.54THH40 pKa = 6.67KK41 pKa = 9.63RR42 pKa = 11.84AHH44 pKa = 5.11HH45 pKa = 4.51QRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84GNSISRR55 pKa = 11.84KK56 pKa = 8.27VLL58 pKa = 3.27

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2046

58

666

292.3

33.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.718 ± 1.01

1.808 ± 0.613

5.865 ± 0.499

7.087 ± 0.542

3.666 ± 0.348

7.136 ± 0.691

2.542 ± 0.371

4.594 ± 0.423

6.354 ± 1.266

9.873 ± 0.778

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.483

3.568 ± 0.332

6.256 ± 1.089

3.715 ± 0.492

6.403 ± 0.902

6.598 ± 0.451

4.985 ± 0.935

6.305 ± 0.582

1.808 ± 0.499

3.519 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski