Jannaschia seosinensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Jannaschia

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M7B6C7|A0A0M7B6C7_9RHOB Putative DNA modification/repair radical SAM protein OS=Jannaschia seosinensis OX=313367 GN=JSE7799_00236 PE=4 SV=1
MM1 pKa = 7.44LRR3 pKa = 11.84ILTSTALAATLAAPLAAPAAAEE25 pKa = 4.04THH27 pKa = 4.73MTAGTEE33 pKa = 4.1MMDD36 pKa = 3.24TAGLIRR42 pKa = 11.84TRR44 pKa = 11.84DD45 pKa = 3.27ITGGPVYY52 pKa = 10.58SIADD56 pKa = 4.19RR57 pKa = 11.84YY58 pKa = 11.37DD59 pKa = 3.0EE60 pKa = 4.32TAWMDD65 pKa = 3.82LEE67 pKa = 4.97DD68 pKa = 4.34RR69 pKa = 11.84DD70 pKa = 4.07YY71 pKa = 11.79YY72 pKa = 11.42GYY74 pKa = 11.55NNYY77 pKa = 10.55NYY79 pKa = 9.03GTDD82 pKa = 3.45YY83 pKa = 11.4DD84 pKa = 4.13QIGEE88 pKa = 4.15IEE90 pKa = 5.67DD91 pKa = 3.52IVLDD95 pKa = 4.37QGGQMIGIVAEE106 pKa = 4.03VGGFLDD112 pKa = 4.48IGDD115 pKa = 3.86KK116 pKa = 10.76HH117 pKa = 7.39VLVPVEE123 pKa = 4.55DD124 pKa = 4.02LRR126 pKa = 11.84LVPVDD131 pKa = 3.66DD132 pKa = 4.02TSYY135 pKa = 11.41SYY137 pKa = 9.23ITRR140 pKa = 11.84LSEE143 pKa = 3.74EE144 pKa = 4.21ALEE147 pKa = 4.28EE148 pKa = 4.16LPSVDD153 pKa = 3.12EE154 pKa = 4.91AGWNN158 pKa = 3.48

Molecular weight: 17.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M7BF63|A0A0M7BF63_9RHOB Uncharacterized protein OS=Jannaschia seosinensis OX=313367 GN=JSE7799_03217 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3710

0

3710

1077800

29

3690

290.5

31.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.882 ± 0.062

0.903 ± 0.013

6.211 ± 0.038

6.168 ± 0.039

3.501 ± 0.029

8.626 ± 0.042

2.123 ± 0.024

4.787 ± 0.03

2.688 ± 0.036

9.999 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.022

2.296 ± 0.02

5.35 ± 0.029

2.837 ± 0.017

8.046 ± 0.04

4.806 ± 0.025

5.415 ± 0.024

7.276 ± 0.034

1.501 ± 0.02

1.973 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski