Amino acid dipepetide frequency for Sphingomonas sp. Leaf33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.398AlaAla: 21.398 ± 0.24
1.09AlaCys: 1.09 ± 0.037
8.617AlaAsp: 8.617 ± 0.114
7.206AlaGlu: 7.206 ± 0.113
4.48AlaPhe: 4.48 ± 0.077
12.548AlaGly: 12.548 ± 0.128
2.241AlaHis: 2.241 ± 0.059
7.352AlaIle: 7.352 ± 0.089
4.141AlaLys: 4.141 ± 0.076
14.287AlaLeu: 14.287 ± 0.153
3.987AlaMet: 3.987 ± 0.071
3.078AlaAsn: 3.078 ± 0.056
6.89AlaPro: 6.89 ± 0.118
4.393AlaGln: 4.393 ± 0.072
10.745AlaArg: 10.745 ± 0.146
6.425AlaSer: 6.425 ± 0.098
8.047AlaThr: 8.047 ± 0.095
9.954AlaVal: 9.954 ± 0.113
1.871AlaTrp: 1.871 ± 0.049
2.578AlaTyr: 2.578 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.887CysAla: 0.887 ± 0.035
0.064CysCys: 0.064 ± 0.01
0.492CysAsp: 0.492 ± 0.024
0.315CysGlu: 0.315 ± 0.019
0.24CysPhe: 0.24 ± 0.018
0.801CysGly: 0.801 ± 0.032
0.177CysHis: 0.177 ± 0.014
0.304CysIle: 0.304 ± 0.018
0.137CysLys: 0.137 ± 0.012
0.642CysLeu: 0.642 ± 0.027
0.107CysMet: 0.107 ± 0.01
0.187CysAsn: 0.187 ± 0.016
0.412CysPro: 0.412 ± 0.02
0.149CysGln: 0.149 ± 0.015
0.519CysArg: 0.519 ± 0.025
0.342CysSer: 0.342 ± 0.021
0.385CysThr: 0.385 ± 0.019
0.54CysVal: 0.54 ± 0.022
0.088CysTrp: 0.088 ± 0.009
0.13CysTyr: 0.13 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.333AspAla: 9.333 ± 0.116
0.426AspCys: 0.426 ± 0.023
3.903AspAsp: 3.903 ± 0.076
3.053AspGlu: 3.053 ± 0.06
2.138AspPhe: 2.138 ± 0.049
6.172AspGly: 6.172 ± 0.087
1.289AspHis: 1.289 ± 0.041
2.724AspIle: 2.724 ± 0.05
1.489AspLys: 1.489 ± 0.046
6.003AspLeu: 6.003 ± 0.076
1.395AspMet: 1.395 ± 0.047
1.181AspAsn: 1.181 ± 0.042
3.941AspPro: 3.941 ± 0.072
1.687AspGln: 1.687 ± 0.047
5.805AspArg: 5.805 ± 0.094
2.22AspSer: 2.22 ± 0.041
3.316AspThr: 3.316 ± 0.07
4.791AspVal: 4.791 ± 0.075
1.214AspTrp: 1.214 ± 0.037
1.631AspTyr: 1.631 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
6.969GluAla: 6.969 ± 0.104
0.257GluCys: 0.257 ± 0.017
2.541GluAsp: 2.541 ± 0.06
2.171GluGlu: 2.171 ± 0.056
1.251GluPhe: 1.251 ± 0.039
4.01GluGly: 4.01 ± 0.071
0.954GluHis: 0.954 ± 0.032
2.4GluIle: 2.4 ± 0.049
1.504GluLys: 1.504 ± 0.047
4.291GluLeu: 4.291 ± 0.075
1.261GluMet: 1.261 ± 0.038
1.146GluAsn: 1.146 ± 0.037
2.405GluPro: 2.405 ± 0.047
1.647GluGln: 1.647 ± 0.053
4.84GluArg: 4.84 ± 0.085
1.85GluSer: 1.85 ± 0.042
3.013GluThr: 3.013 ± 0.064
3.333GluVal: 3.333 ± 0.064
0.691GluTrp: 0.691 ± 0.028
0.854GluTyr: 0.854 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.908PheAla: 4.908 ± 0.081
0.241PheCys: 0.241 ± 0.016
2.723PheAsp: 2.723 ± 0.056
1.806PheGlu: 1.806 ± 0.039
1.137PhePhe: 1.137 ± 0.041
3.483PheGly: 3.483 ± 0.073
0.655PheHis: 0.655 ± 0.028
1.225PheIle: 1.225 ± 0.036
0.765PheLys: 0.765 ± 0.028
2.966PheLeu: 2.966 ± 0.059
0.578PheMet: 0.578 ± 0.026
0.906PheAsn: 0.906 ± 0.037
1.389PhePro: 1.389 ± 0.037
0.808PheGln: 0.808 ± 0.034
2.172PheArg: 2.172 ± 0.05
1.746PheSer: 1.746 ± 0.043
2.017PheThr: 2.017 ± 0.047
2.819PheVal: 2.819 ± 0.055
0.458PheTrp: 0.458 ± 0.025
0.822PheTyr: 0.822 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
10.663GlyAla: 10.663 ± 0.133
0.816GlyCys: 0.816 ± 0.032
5.551GlyAsp: 5.551 ± 0.091
4.482GlyGlu: 4.482 ± 0.072
3.581GlyPhe: 3.581 ± 0.073
8.658GlyGly: 8.658 ± 0.177
1.854GlyHis: 1.854 ± 0.053
4.53GlyIle: 4.53 ± 0.08
2.939GlyLys: 2.939 ± 0.071
8.584GlyLeu: 8.584 ± 0.095
2.26GlyMet: 2.26 ± 0.051
2.13GlyAsn: 2.13 ± 0.07
3.629GlyPro: 3.629 ± 0.061
2.871GlyGln: 2.871 ± 0.058
7.113GlyArg: 7.113 ± 0.099
4.611GlySer: 4.611 ± 0.099
5.47GlyThr: 5.47 ± 0.107
6.964GlyVal: 6.964 ± 0.101
1.75GlyTrp: 1.75 ± 0.052
2.368GlyTyr: 2.368 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.506HisAla: 2.506 ± 0.065
0.176HisCys: 0.176 ± 0.013
1.257HisAsp: 1.257 ± 0.037
0.866HisGlu: 0.866 ± 0.033
0.681HisPhe: 0.681 ± 0.024
1.901HisGly: 1.901 ± 0.05
0.541HisHis: 0.541 ± 0.023
0.844HisIle: 0.844 ± 0.034
0.334HisLys: 0.334 ± 0.02
1.765HisLeu: 1.765 ± 0.048
0.402HisMet: 0.402 ± 0.021
0.374HisAsn: 0.374 ± 0.02
1.255HisPro: 1.255 ± 0.037
0.427HisGln: 0.427 ± 0.02
1.473HisArg: 1.473 ± 0.046
0.757HisSer: 0.757 ± 0.03
0.69HisThr: 0.69 ± 0.028
1.411HisVal: 1.411 ± 0.038
0.293HisTrp: 0.293 ± 0.015
0.454HisTyr: 0.454 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
8.39IleAla: 8.39 ± 0.092
0.333IleCys: 0.333 ± 0.021
4.261IleAsp: 4.261 ± 0.074
2.997IleGlu: 2.997 ± 0.058
1.322IlePhe: 1.322 ± 0.041
5.14IleGly: 5.14 ± 0.068
0.778IleHis: 0.778 ± 0.031
1.61IleIle: 1.61 ± 0.047
0.969IleLys: 0.969 ± 0.039
3.929IleLeu: 3.929 ± 0.067
0.655IleMet: 0.655 ± 0.025
1.095IleAsn: 1.095 ± 0.039
2.191IlePro: 2.191 ± 0.045
1.075IleGln: 1.075 ± 0.032
3.178IleArg: 3.178 ± 0.056
1.949IleSer: 1.949 ± 0.047
2.411IleThr: 2.411 ± 0.059
4.49IleVal: 4.49 ± 0.064
0.536IleTrp: 0.536 ± 0.026
0.844IleTyr: 0.844 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
3.693LysAla: 3.693 ± 0.078
0.126LysCys: 0.126 ± 0.011
1.493LysAsp: 1.493 ± 0.043
0.903LysGlu: 0.903 ± 0.036
0.73LysPhe: 0.73 ± 0.027
2.386LysGly: 2.386 ± 0.058
0.427LysHis: 0.427 ± 0.024
1.167LysIle: 1.167 ± 0.04
0.9LysLys: 0.9 ± 0.046
2.909LysLeu: 2.909 ± 0.061
0.676LysMet: 0.676 ± 0.027
0.567LysAsn: 0.567 ± 0.029
1.834LysPro: 1.834 ± 0.044
0.714LysGln: 0.714 ± 0.028
2.128LysArg: 2.128 ± 0.049
1.389LysSer: 1.389 ± 0.039
1.667LysThr: 1.667 ± 0.043
1.958LysVal: 1.958 ± 0.048
0.347LysTrp: 0.347 ± 0.02
0.541LysTyr: 0.541 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.667LeuAla: 14.667 ± 0.162
0.629LeuCys: 0.629 ± 0.027
6.356LeuAsp: 6.356 ± 0.085
4.003LeuGlu: 4.003 ± 0.08
3.317LeuPhe: 3.317 ± 0.068
8.266LeuGly: 8.266 ± 0.11
1.675LeuHis: 1.675 ± 0.043
4.522LeuIle: 4.522 ± 0.068
2.831LeuLys: 2.831 ± 0.057
9.05LeuLeu: 9.05 ± 0.139
2.005LeuMet: 2.005 ± 0.05
2.202LeuAsn: 2.202 ± 0.057
5.686LeuPro: 5.686 ± 0.078
2.249LeuGln: 2.249 ± 0.054
6.809LeuArg: 6.809 ± 0.099
5.835LeuSer: 5.835 ± 0.09
6.235LeuThr: 6.235 ± 0.085
7.567LeuVal: 7.567 ± 0.094
1.264LeuTrp: 1.264 ± 0.045
1.987LeuTyr: 1.987 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
3.18MetAla: 3.18 ± 0.062
0.126MetCys: 0.126 ± 0.01
1.024MetAsp: 1.024 ± 0.036
0.849MetGlu: 0.849 ± 0.033
0.665MetPhe: 0.665 ± 0.029
1.808MetGly: 1.808 ± 0.048
0.365MetHis: 0.365 ± 0.018
1.317MetIle: 1.317 ± 0.039
0.805MetLys: 0.805 ± 0.031
2.546MetLeu: 2.546 ± 0.06
0.562MetMet: 0.562 ± 0.028
0.547MetAsn: 0.547 ± 0.026
1.37MetPro: 1.37 ± 0.036
0.622MetGln: 0.622 ± 0.027
1.861MetArg: 1.861 ± 0.045
1.277MetSer: 1.277 ± 0.034
1.984MetThr: 1.984 ± 0.039
1.621MetVal: 1.621 ± 0.048
0.215MetTrp: 0.215 ± 0.016
0.271MetTyr: 0.271 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.058AsnAla: 3.058 ± 0.064
0.174AsnCys: 0.174 ± 0.014
1.444AsnAsp: 1.444 ± 0.06
0.846AsnGlu: 0.846 ± 0.028
0.871AsnPhe: 0.871 ± 0.035
2.224AsnGly: 2.224 ± 0.07
0.428AsnHis: 0.428 ± 0.024
1.131AsnIle: 1.131 ± 0.036
0.483AsnLys: 0.483 ± 0.025
2.261AsnLeu: 2.261 ± 0.057
0.431AsnMet: 0.431 ± 0.022
0.603AsnAsn: 0.603 ± 0.03
1.664AsnPro: 1.664 ± 0.046
0.654AsnGln: 0.654 ± 0.027
1.806AsnArg: 1.806 ± 0.045
1.071AsnSer: 1.071 ± 0.042
1.179AsnThr: 1.179 ± 0.049
1.748AsnVal: 1.748 ± 0.049
0.373AsnTrp: 0.373 ± 0.02
0.605AsnTyr: 0.605 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
7.852ProAla: 7.852 ± 0.128
0.297ProCys: 0.297 ± 0.017
3.951ProAsp: 3.951 ± 0.067
3.058ProGlu: 3.058 ± 0.067
1.887ProPhe: 1.887 ± 0.043
4.931ProGly: 4.931 ± 0.083
0.984ProHis: 0.984 ± 0.031
2.475ProIle: 2.475 ± 0.054
1.477ProLys: 1.477 ± 0.037
4.86ProLeu: 4.86 ± 0.079
1.2ProMet: 1.2 ± 0.039
1.281ProAsn: 1.281 ± 0.037
3.004ProPro: 3.004 ± 0.09
1.682ProGln: 1.682 ± 0.049
3.387ProArg: 3.387 ± 0.062
2.565ProSer: 2.565 ± 0.047
3.047ProThr: 3.047 ± 0.062
4.655ProVal: 4.655 ± 0.073
0.684ProTrp: 0.684 ± 0.029
1.101ProTyr: 1.101 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
4.166GlnAla: 4.166 ± 0.065
0.162GlnCys: 0.162 ± 0.014
1.406GlnAsp: 1.406 ± 0.037
0.963GlnGlu: 0.963 ± 0.037
0.935GlnPhe: 0.935 ± 0.036
2.327GlnGly: 2.327 ± 0.053
0.513GlnHis: 0.513 ± 0.026
1.479GlnIle: 1.479 ± 0.041
0.742GlnLys: 0.742 ± 0.028
2.783GlnLeu: 2.783 ± 0.051
0.751GlnMet: 0.751 ± 0.029
0.654GlnAsn: 0.654 ± 0.028
1.73GlnPro: 1.73 ± 0.041
0.984GlnGln: 0.984 ± 0.038
2.431GlnArg: 2.431 ± 0.054
1.508GlnSer: 1.508 ± 0.04
1.64GlnThr: 1.64 ± 0.044
2.294GlnVal: 2.294 ± 0.052
0.364GlnTrp: 0.364 ± 0.021
0.546GlnTyr: 0.546 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
10.006ArgAla: 10.006 ± 0.143
0.474ArgCys: 0.474 ± 0.023
5.03ArgAsp: 5.03 ± 0.079
3.756ArgGlu: 3.756 ± 0.07
3.123ArgPhe: 3.123 ± 0.062
5.664ArgGly: 5.664 ± 0.087
1.666ArgHis: 1.666 ± 0.048
4.378ArgIle: 4.378 ± 0.072
1.704ArgLys: 1.704 ± 0.046
8.271ArgLeu: 8.271 ± 0.113
2.083ArgMet: 2.083 ± 0.049
1.695ArgAsn: 1.695 ± 0.047
4.042ArgPro: 4.042 ± 0.081
2.344ArgGln: 2.344 ± 0.054
6.442ArgArg: 6.442 ± 0.114
3.545ArgSer: 3.545 ± 0.067
4.275ArgThr: 4.275 ± 0.07
5.718ArgVal: 5.718 ± 0.079
1.302ArgTrp: 1.302 ± 0.038
1.89ArgTyr: 1.89 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
6.164SerAla: 6.164 ± 0.088
0.334SerCys: 0.334 ± 0.019
3.033SerAsp: 3.033 ± 0.052
2.059SerGlu: 2.059 ± 0.051
1.892SerPhe: 1.892 ± 0.048
5.234SerGly: 5.234 ± 0.089
0.797SerHis: 0.797 ± 0.029
2.473SerIle: 2.473 ± 0.053
1.221SerLys: 1.221 ± 0.039
4.569SerLeu: 4.569 ± 0.076
1.06SerMet: 1.06 ± 0.033
1.26SerAsn: 1.26 ± 0.047
2.79SerPro: 2.79 ± 0.056
1.312SerGln: 1.312 ± 0.039
3.313SerArg: 3.313 ± 0.063
2.469SerSer: 2.469 ± 0.066
2.666SerThr: 2.666 ± 0.063
3.806SerVal: 3.806 ± 0.063
0.649SerTrp: 0.649 ± 0.025
1.177SerTyr: 1.177 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.743ThrAla: 7.743 ± 0.098
0.375ThrCys: 0.375 ± 0.023
3.427ThrAsp: 3.427 ± 0.067
2.178ThrGlu: 2.178 ± 0.044
1.763ThrPhe: 1.763 ± 0.053
5.933ThrGly: 5.933 ± 0.1
0.998ThrHis: 0.998 ± 0.039
3.247ThrIle: 3.247 ± 0.061
1.337ThrLys: 1.337 ± 0.038
6.67ThrLeu: 6.67 ± 0.088
1.285ThrMet: 1.285 ± 0.035
1.308ThrAsn: 1.308 ± 0.05
4.067ThrPro: 4.067 ± 0.079
1.574ThrGln: 1.574 ± 0.042
4.092ThrArg: 4.092 ± 0.063
2.756ThrSer: 2.756 ± 0.071
3.425ThrThr: 3.425 ± 0.087
4.698ThrVal: 4.698 ± 0.079
0.668ThrTrp: 0.668 ± 0.027
1.183ThrTyr: 1.183 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
11.51ValAla: 11.51 ± 0.135
0.513ValCys: 0.513 ± 0.024
4.816ValAsp: 4.816 ± 0.071
4.413ValGlu: 4.413 ± 0.078
2.284ValPhe: 2.284 ± 0.046
6.26ValGly: 6.26 ± 0.103
1.317ValHis: 1.317 ± 0.039
3.563ValIle: 3.563 ± 0.06
1.944ValLys: 1.944 ± 0.052
7.058ValLeu: 7.058 ± 0.088
1.605ValMet: 1.605 ± 0.043
1.87ValAsn: 1.87 ± 0.054
4.343ValPro: 4.343 ± 0.06
2.036ValGln: 2.036 ± 0.055
5.84ValArg: 5.84 ± 0.095
3.974ValSer: 3.974 ± 0.072
5.254ValThr: 5.254 ± 0.084
6.319ValVal: 6.319 ± 0.099
0.931ValTrp: 0.931 ± 0.033
1.338ValTyr: 1.338 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.525TrpAla: 1.525 ± 0.045
0.12TrpCys: 0.12 ± 0.01
0.774TrpAsp: 0.774 ± 0.033
0.51TrpGlu: 0.51 ± 0.025
0.562TrpPhe: 0.562 ± 0.027
0.983TrpGly: 0.983 ± 0.035
0.31TrpHis: 0.31 ± 0.018
0.635TrpIle: 0.635 ± 0.027
0.358TrpLys: 0.358 ± 0.021
1.675TrpLeu: 1.675 ± 0.047
0.35TrpMet: 0.35 ± 0.018
0.426TrpAsn: 0.426 ± 0.023
0.713TrpPro: 0.713 ± 0.028
0.528TrpGln: 0.528 ± 0.022
1.431TrpArg: 1.431 ± 0.043
0.948TrpSer: 0.948 ± 0.033
0.941TrpThr: 0.941 ± 0.032
0.936TrpVal: 0.936 ± 0.035
0.273TrpTrp: 0.273 ± 0.017
0.297TrpTyr: 0.297 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.707TyrAla: 2.707 ± 0.062
0.188TyrCys: 0.188 ± 0.013
1.613TyrAsp: 1.613 ± 0.046
0.955TyrGlu: 0.955 ± 0.033
0.766TyrPhe: 0.766 ± 0.033
2.094TyrGly: 2.094 ± 0.053
0.438TyrHis: 0.438 ± 0.023
0.778TyrIle: 0.778 ± 0.029
0.476TyrLys: 0.476 ± 0.022
2.035TyrLeu: 2.035 ± 0.047
0.363TyrMet: 0.363 ± 0.02
0.546TyrAsn: 0.546 ± 0.026
1.021TyrPro: 1.021 ± 0.037
0.618TyrGln: 0.618 ± 0.026
2.022TyrArg: 2.022 ± 0.045
1.04TyrSer: 1.04 ± 0.039
1.065TyrThr: 1.065 ± 0.038
1.567TyrVal: 1.567 ± 0.042
0.325TyrTrp: 0.325 ± 0.018
0.596TyrTyr: 0.596 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2982 proteins (943339 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski