Brevibacillus phage Sundance

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FM51|A0A0K2FM51_9CAUD Uncharacterized protein OS=Brevibacillus phage Sundance OX=1691958 GN=SUNDANCE_83 PE=4 SV=1
MM1 pKa = 7.24TMSIVKK7 pKa = 10.11SALFLEE13 pKa = 4.78AVLMLQKK20 pKa = 10.71VYY22 pKa = 10.58LVHH25 pKa = 7.28DD26 pKa = 4.12FLYY29 pKa = 10.96GSLDD33 pKa = 4.02SIPDD37 pKa = 3.27EE38 pKa = 4.39SLDD41 pKa = 3.69GKK43 pKa = 8.99STIDD47 pKa = 3.99EE48 pKa = 3.98IWIVTDD54 pKa = 3.57VPIVEE59 pKa = 4.91ADD61 pKa = 3.44SNDD64 pKa = 3.27YY65 pKa = 10.85YY66 pKa = 10.39IYY68 pKa = 10.53HH69 pKa = 6.02SEE71 pKa = 3.99KK72 pKa = 10.01EE73 pKa = 4.24AYY75 pKa = 8.79EE76 pKa = 4.28SQVKK80 pKa = 10.11YY81 pKa = 10.78DD82 pKa = 3.33

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FLH2|A0A0K2FLH2_9CAUD Uncharacterized protein OS=Brevibacillus phage Sundance OX=1691958 GN=SUNDANCE_165 PE=4 SV=1
MM1 pKa = 7.67ANGGFVRR8 pKa = 11.84KK9 pKa = 9.71NGYY12 pKa = 9.33LAVLHH17 pKa = 7.21RR18 pKa = 11.84DD19 pKa = 3.19TVIPMRR25 pKa = 11.84YY26 pKa = 8.56VEE28 pKa = 4.06EE29 pKa = 4.3LKK31 pKa = 10.78RR32 pKa = 11.84VAKK35 pKa = 10.24RR36 pKa = 11.84VAKK39 pKa = 10.11RR40 pKa = 11.84VAEE43 pKa = 4.01QKK45 pKa = 10.31QFSSSITINIKK56 pKa = 10.3

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

194

0

194

40393

29

2528

208.2

23.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.922 ± 0.167

0.933 ± 0.081

6.365 ± 0.165

8.147 ± 0.287

4.471 ± 0.151

5.907 ± 0.186

1.686 ± 0.093

7.68 ± 0.175

9.175 ± 0.284

7.907 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.1

6.214 ± 0.179

2.369 ± 0.123

3.389 ± 0.172

4.127 ± 0.121

6.031 ± 0.163

5.325 ± 0.168

6.61 ± 0.178

1.154 ± 0.074

4.79 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski