Blackcurrant leafroll-associated virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus; unclassified Closterovirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FH38|A0A346FH38_9CLOS P14 OS=Blackcurrant leafroll-associated virus 1 OX=2292426 PE=4 SV=1
MM1 pKa = 7.41AAGVRR6 pKa = 11.84KK7 pKa = 8.09TPIIFIDD14 pKa = 4.62ISTSWIGYY22 pKa = 8.69RR23 pKa = 11.84DD24 pKa = 3.67CLINCDD30 pKa = 3.38HH31 pKa = 6.96MIIADD36 pKa = 5.38CEE38 pKa = 4.42YY39 pKa = 10.43EE40 pKa = 4.25WNCIDD45 pKa = 3.77GDD47 pKa = 4.01CWEE50 pKa = 4.91SPTASVVEE58 pKa = 4.74FFDD61 pKa = 4.85SCIEE65 pKa = 4.12DD66 pKa = 3.51PGVQVVNVSRR76 pKa = 11.84NDD78 pKa = 4.05LISSKK83 pKa = 10.99VIVEE87 pKa = 4.09YY88 pKa = 10.35TSTFTIYY95 pKa = 10.21HH96 pKa = 5.74GSDD99 pKa = 2.61RR100 pKa = 11.84RR101 pKa = 11.84TVVTFDD107 pKa = 3.11STPVVNRR114 pKa = 11.84SMIYY118 pKa = 9.12TEE120 pKa = 4.18SSPVLSLVNILKK132 pKa = 8.95TLDD135 pKa = 3.16SWKK138 pKa = 10.17LKK140 pKa = 10.05QVTVVNLL147 pKa = 3.41

Molecular weight: 16.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FH37|A0A346FH37_9CLOS P17 OS=Blackcurrant leafroll-associated virus 1 OX=2292426 PE=4 SV=1
MM1 pKa = 7.18STGKK5 pKa = 9.79PPLTLRR11 pKa = 11.84DD12 pKa = 3.42ISRR15 pKa = 11.84VSIKK19 pKa = 10.32LDD21 pKa = 3.21KK22 pKa = 10.65EE23 pKa = 4.09LNEE26 pKa = 4.08VEE28 pKa = 4.38KK29 pKa = 10.26TVVLAGLVKK38 pKa = 10.29IFAEE42 pKa = 4.85EE43 pKa = 4.08YY44 pKa = 9.67KK45 pKa = 11.02FPVEE49 pKa = 4.83DD50 pKa = 3.62INMHH54 pKa = 6.01VYY56 pKa = 10.21MIMPLLNRR64 pKa = 11.84VSTSDD69 pKa = 3.63KK70 pKa = 10.77VVASQAGSGITYY82 pKa = 9.02TLGDD86 pKa = 3.42KK87 pKa = 10.44EE88 pKa = 4.26YY89 pKa = 10.21TIKK92 pKa = 10.92EE93 pKa = 4.42DD94 pKa = 3.29ILEE97 pKa = 4.87KK98 pKa = 10.3ISQLVPRR105 pKa = 11.84TGKK108 pKa = 10.33NNLRR112 pKa = 11.84LWARR116 pKa = 11.84TCEE119 pKa = 4.04KK120 pKa = 10.71VYY122 pKa = 10.98LDD124 pKa = 3.77VAFAQPDD131 pKa = 3.61LFKK134 pKa = 11.06CEE136 pKa = 3.58RR137 pKa = 11.84SLRR140 pKa = 11.84ANAPIGFAWAAADD153 pKa = 3.94FLPGVDD159 pKa = 4.55RR160 pKa = 11.84RR161 pKa = 11.84LTDD164 pKa = 3.11EE165 pKa = 3.72MRR167 pKa = 11.84AAIVSCRR174 pKa = 11.84KK175 pKa = 7.67TLLRR179 pKa = 11.84RR180 pKa = 11.84SKK182 pKa = 10.48EE183 pKa = 3.75VSEE186 pKa = 4.69TGNQLITLEE195 pKa = 4.2KK196 pKa = 10.59LGVASS201 pKa = 4.17

Molecular weight: 22.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5356

49

2839

535.6

60.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.676 ± 0.304

2.707 ± 0.408

6.255 ± 0.356

5.377 ± 0.466

5.825 ± 0.435

5.583 ± 0.386

1.848 ± 0.289

5.639 ± 0.356

6.311 ± 0.332

9.727 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.755 ± 0.166

4.238 ± 0.348

3.734 ± 0.244

2.315 ± 0.156

5.956 ± 0.275

9.989 ± 1.052

4.985 ± 0.335

7.935 ± 0.285

0.635 ± 0.194

3.51 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski