Roseobacter phage CRP-7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; unclassified Caudovirales

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QWN6|A0A646QWN6_9CAUD Putative tail structural protein OS=Roseobacter phage CRP-7 OX=2559286 GN=CRP7_gp48 PE=4 SV=1
MM1 pKa = 7.81ALTDD5 pKa = 3.61ILYY8 pKa = 9.05TLIAVVMFQAYY19 pKa = 8.7QIWNMQRR26 pKa = 11.84RR27 pKa = 11.84YY28 pKa = 10.85EE29 pKa = 4.03EE30 pKa = 4.26MLNLMIGLHH39 pKa = 5.87LGEE42 pKa = 4.95INLEE46 pKa = 4.07EE47 pKa = 4.72VDD49 pKa = 4.5NDD51 pKa = 4.02DD52 pKa = 5.09DD53 pKa = 4.14

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QWU1|A0A646QWU1_9CAUD Putative tail structural protein OS=Roseobacter phage CRP-7 OX=2559286 GN=CRP7_gp51 PE=4 SV=1
MM1 pKa = 7.65AATPNKK7 pKa = 9.67GKK9 pKa = 10.8AKK11 pKa = 10.33VKK13 pKa = 9.12VTASGKK19 pKa = 9.36RR20 pKa = 11.84VSYY23 pKa = 10.25GQAGKK28 pKa = 10.57AKK30 pKa = 10.0DD31 pKa = 3.38GSARR35 pKa = 11.84VQPSSKK41 pKa = 10.68KK42 pKa = 10.15GDD44 pKa = 3.59AYY46 pKa = 10.58CARR49 pKa = 11.84SAGQKK54 pKa = 9.53KK55 pKa = 9.92RR56 pKa = 11.84SPKK59 pKa = 9.72AAKK62 pKa = 10.19DD63 pKa = 3.51PNSPLNLSRR72 pKa = 11.84KK73 pKa = 7.8RR74 pKa = 11.84WKK76 pKa = 10.79CSGTKK81 pKa = 9.62SKK83 pKa = 11.0RR84 pKa = 3.31

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

18381

43

1368

255.3

28.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.015 ± 0.553

0.615 ± 0.109

6.175 ± 0.209

6.692 ± 0.382

3.03 ± 0.154

7.426 ± 0.405

1.458 ± 0.16

5.93 ± 0.229

6.365 ± 0.422

7.415 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.209

6.387 ± 0.343

3.618 ± 0.4

3.77 ± 0.278

4.162 ± 0.196

7.361 ± 0.382

7.219 ± 0.465

5.821 ± 0.222

1.24 ± 0.115

3.602 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski