Frondihabitans sp. Leaf304

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frondihabitans; unclassified Frondihabitans

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3039 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5C9H5|A0A0Q5C9H5_9MICO DNA polymerase III subunit gamma/tau OS=Frondihabitans sp. Leaf304 OX=1736329 GN=ASF54_05370 PE=3 SV=1
MM1 pKa = 7.64TSTSDD6 pKa = 3.17AQLVDD11 pKa = 3.67GFSVKK16 pKa = 7.8MTVPASFPAGEE27 pKa = 4.63AYY29 pKa = 10.42AEE31 pKa = 4.11IAGWDD36 pKa = 3.55YY37 pKa = 11.69SLCNDD42 pKa = 4.12NGSCASPSGSFIVSAGQQ59 pKa = 2.95

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5CNV2|A0A0Q5CNV2_9MICO Amidase domain-containing protein OS=Frondihabitans sp. Leaf304 OX=1736329 GN=ASF54_10265 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.23VRR4 pKa = 11.84NSLASLKK11 pKa = 9.97KK12 pKa = 10.49LPGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 8.63LNPRR34 pKa = 11.84GKK36 pKa = 10.33GRR38 pKa = 11.84QGG40 pKa = 2.91

Molecular weight: 4.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3039

0

3039

971049

38

3048

319.5

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.913 ± 0.062

0.439 ± 0.009

6.2 ± 0.041

5.201 ± 0.045

3.217 ± 0.025

8.911 ± 0.042

1.879 ± 0.02

4.534 ± 0.033

2.431 ± 0.039

10.107 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.617 ± 0.019

2.068 ± 0.027

5.216 ± 0.032

2.75 ± 0.023

6.783 ± 0.058

6.631 ± 0.041

6.63 ± 0.06

9.091 ± 0.045

1.379 ± 0.017

2.002 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski