Lake Sarah-associated circular virus-7

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9J8|A0A126G9J8_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-7 OX=1685784 PE=3 SV=1
MM1 pKa = 7.28FCYY4 pKa = 10.02IKK6 pKa = 10.83NAISCLRR13 pKa = 11.84TVKK16 pKa = 10.47INVFSMPPSRR26 pKa = 11.84SRR28 pKa = 11.84AFIFTWNNPTADD40 pKa = 3.45TEE42 pKa = 4.25AALEE46 pKa = 4.11SLAGYY51 pKa = 10.25SYY53 pKa = 10.45LTFGRR58 pKa = 11.84EE59 pKa = 3.97TAPTTGTRR67 pKa = 11.84HH68 pKa = 4.55LQGYY72 pKa = 9.48IRR74 pKa = 11.84FTDD77 pKa = 3.97GKK79 pKa = 8.54SLRR82 pKa = 11.84SARR85 pKa = 11.84RR86 pKa = 11.84LLNGAHH92 pKa = 5.81VEE94 pKa = 4.11VARR97 pKa = 11.84TIRR100 pKa = 11.84QAIEE104 pKa = 3.97YY105 pKa = 8.48CHH107 pKa = 6.85KK108 pKa = 10.79EE109 pKa = 3.42GDD111 pKa = 3.73FVEE114 pKa = 5.5FGTRR118 pKa = 11.84PVDD121 pKa = 3.44DD122 pKa = 4.14AARR125 pKa = 11.84GDD127 pKa = 3.69MEE129 pKa = 4.09KK130 pKa = 10.76ARR132 pKa = 11.84WEE134 pKa = 4.21IAWTKK139 pKa = 11.1AKK141 pKa = 9.47TADD144 pKa = 4.21LEE146 pKa = 4.78EE147 pKa = 5.58IDD149 pKa = 3.39ADD151 pKa = 3.45IRR153 pKa = 11.84VRR155 pKa = 11.84CYY157 pKa = 10.64SALTRR162 pKa = 11.84IQKK165 pKa = 10.71DD166 pKa = 3.71YY167 pKa = 10.45MVPPLPLPAPCGLWIHH183 pKa = 6.2GLSGVGKK190 pKa = 8.13TFAVYY195 pKa = 9.37QAYY198 pKa = 9.24PDD200 pKa = 4.75LYY202 pKa = 11.09SKK204 pKa = 10.72NASKK208 pKa = 9.19WWDD211 pKa = 3.54GYY213 pKa = 10.29QNQDD217 pKa = 3.67HH218 pKa = 6.88ILFDD222 pKa = 5.38DD223 pKa = 3.9MDD225 pKa = 5.64PDD227 pKa = 3.56VGKK230 pKa = 9.39WAGRR234 pKa = 11.84FFKK237 pKa = 10.04IWADD241 pKa = 3.57EE242 pKa = 4.29RR243 pKa = 11.84PFIADD248 pKa = 3.06IKK250 pKa = 10.43GGSISIRR257 pKa = 11.84PKK259 pKa = 10.06IFIVTSQYY267 pKa = 11.0TIDD270 pKa = 3.52EE271 pKa = 4.61CFGEE275 pKa = 4.21IQTRR279 pKa = 11.84MALSRR284 pKa = 11.84RR285 pKa = 11.84FRR287 pKa = 11.84IIEE290 pKa = 4.15KK291 pKa = 10.42LSLDD295 pKa = 3.55HH296 pKa = 7.19EE297 pKa = 4.92INII300 pKa = 4.65

Molecular weight: 34.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9J8|A0A126G9J8_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-7 OX=1685784 PE=3 SV=1
MM1 pKa = 7.34GRR3 pKa = 11.84RR4 pKa = 11.84FRR6 pKa = 11.84KK7 pKa = 9.01RR8 pKa = 11.84GKK10 pKa = 10.17VIQTTPQGQRR20 pKa = 11.84TIPYY24 pKa = 8.2NASASRR30 pKa = 11.84AAFKK34 pKa = 10.97VADD37 pKa = 4.41AAGKK41 pKa = 9.14WLGKK45 pKa = 9.27KK46 pKa = 9.9AKK48 pKa = 9.98AYY50 pKa = 8.26WNSPTPKK57 pKa = 9.93VYY59 pKa = 10.3KK60 pKa = 10.28KK61 pKa = 10.16KK62 pKa = 10.67SKK64 pKa = 10.18PKK66 pKa = 9.4EE67 pKa = 3.79EE68 pKa = 3.97AKK70 pKa = 10.44EE71 pKa = 3.7LHH73 pKa = 6.12TSVNGGGNVINTIVNIGKK91 pKa = 9.52SNKK94 pKa = 9.21KK95 pKa = 7.3QAKK98 pKa = 9.24LIKK101 pKa = 10.24KK102 pKa = 9.45LGAYY106 pKa = 6.59TTSQYY111 pKa = 11.18LAAFTHH117 pKa = 6.15VNTATEE123 pKa = 3.93ASTQEE128 pKa = 4.13VNDD131 pKa = 3.3VAVYY135 pKa = 10.37LKK137 pKa = 10.65GQSLWDD143 pKa = 3.42IVQNAYY149 pKa = 8.36QQNAAWQALAPNLTTAGAQARR170 pKa = 11.84IYY172 pKa = 10.56VSYY175 pKa = 10.48IKK177 pKa = 10.04TEE179 pKa = 3.79IEE181 pKa = 4.12FVNLSPAICFVDD193 pKa = 3.89IYY195 pKa = 10.84LFRR198 pKa = 11.84AKK200 pKa = 10.13VDD202 pKa = 3.35QLAFSDD208 pKa = 3.83VTSAYY213 pKa = 10.45NLPSNTWATSIDD225 pKa = 3.68QEE227 pKa = 4.35KK228 pKa = 10.91GLGSVSQTFPGMEE241 pKa = 3.71PRR243 pKa = 11.84GALFKK248 pKa = 10.92KK249 pKa = 9.84HH250 pKa = 5.87FKK252 pKa = 9.97EE253 pKa = 4.48VYY255 pKa = 10.02KK256 pKa = 10.6LRR258 pKa = 11.84CCMNGGEE265 pKa = 4.23VRR267 pKa = 11.84RR268 pKa = 11.84LSIYY272 pKa = 8.85MHH274 pKa = 5.48MNKK277 pKa = 8.95WVEE280 pKa = 3.94SAKK283 pKa = 10.53IILNKK288 pKa = 10.53GNLNGLTHH296 pKa = 6.91HH297 pKa = 6.69LMFVEE302 pKa = 4.82RR303 pKa = 11.84GSPCGNLNDD312 pKa = 4.97PYY314 pKa = 11.4VADD317 pKa = 3.83TQVYY321 pKa = 9.84LSPIKK326 pKa = 10.7INGTVNTTFGVGCLVRR342 pKa = 11.84PSNNAYY348 pKa = 9.26TSQTGLTTSYY358 pKa = 10.64AANVYY363 pKa = 8.85TLVDD367 pKa = 3.98DD368 pKa = 5.92DD369 pKa = 5.67GGTKK373 pKa = 10.56NIGVVDD379 pKa = 3.67SFGG382 pKa = 3.22

Molecular weight: 41.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

682

300

382

341.0

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.238 ± 0.152

1.613 ± 0.248

4.985 ± 1.29

4.252 ± 0.692

4.252 ± 0.692

7.185 ± 0.545

1.76 ± 0.154

6.598 ± 1.325

7.185 ± 1.186

6.598 ± 0.17

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.76 ± 0.154

5.279 ± 1.673

4.106 ± 0.359

3.666 ± 0.64

5.572 ± 1.555

6.158 ± 0.315

7.185 ± 0.545

6.158 ± 1.382

2.053 ± 0.393

4.399 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski