Mahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacterales Family IV. Incertae Sedis; Mahella; Mahella australiensis

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2858 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4A0V1|F4A0V1_MAHA5 Uroporphyrinogen_deCOase domain-containing protein OS=Mahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON) OX=697281 GN=Mahau_1819 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.51ASVDD6 pKa = 3.55PEE8 pKa = 4.23LCISCGVCIDD18 pKa = 3.83ICPEE22 pKa = 3.76VFDD25 pKa = 4.7WDD27 pKa = 4.22DD28 pKa = 4.28DD29 pKa = 4.29GLSHH33 pKa = 7.17VIVDD37 pKa = 5.03EE38 pKa = 4.4IPEE41 pKa = 4.08EE42 pKa = 4.48LEE44 pKa = 4.1DD45 pKa = 4.46TVDD48 pKa = 3.59EE49 pKa = 5.17AANQCPTEE57 pKa = 4.81AILVEE62 pKa = 4.14RR63 pKa = 11.84TAFSRR68 pKa = 11.84KK69 pKa = 8.75

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4A144|F4A144_MAHA5 Transferase hexapeptide repeat containing protein OS=Mahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON) OX=697281 GN=Mahau_0747 PE=4 SV=1
MM1 pKa = 7.26LQTYY5 pKa = 7.4QPKK8 pKa = 9.42KK9 pKa = 7.87RR10 pKa = 11.84HH11 pKa = 4.99RR12 pKa = 11.84QRR14 pKa = 11.84VHH16 pKa = 7.21GFMARR21 pKa = 11.84MSTKK25 pKa = 10.34NGRR28 pKa = 11.84KK29 pKa = 7.4VLKK32 pKa = 10.16RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.33GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2858

0

2858

929707

30

3295

325.3

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.092 ± 0.05

1.085 ± 0.017

6.418 ± 0.04

6.155 ± 0.041

3.773 ± 0.03

7.156 ± 0.044

1.731 ± 0.02

8.458 ± 0.05

6.063 ± 0.037

8.782 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.157 ± 0.022

4.42 ± 0.032

3.957 ± 0.028

3.373 ± 0.026

4.614 ± 0.036

5.683 ± 0.036

4.871 ± 0.037

6.97 ± 0.035

1.166 ± 0.023

4.076 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski