Apis cerana cerana (Oriental honeybee)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; Apinae; Apini;

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9931 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3ECG4|A0A2A3ECG4_APICC Iporin OS=Apis cerana cerana OX=94128 GN=APICC_06640 PE=4 SV=1
MM1 pKa = 7.11VVLSNFLAPQEE12 pKa = 4.56GIRR15 pKa = 11.84HH16 pKa = 5.6EE17 pKa = 4.32EE18 pKa = 3.96QNTTVYY24 pKa = 10.76INDD27 pKa = 3.7RR28 pKa = 11.84EE29 pKa = 4.38VGKK32 pKa = 8.77GTLYY36 pKa = 9.3ITEE39 pKa = 4.71SLLSWVNYY47 pKa = 7.76DD48 pKa = 3.57TQQGFSLEE56 pKa = 4.16YY57 pKa = 10.02PHH59 pKa = 7.27ISLHH63 pKa = 6.32AISRR67 pKa = 11.84DD68 pKa = 3.7EE69 pKa = 3.97QVHH72 pKa = 5.94PRR74 pKa = 11.84QCLYY78 pKa = 10.85IMVDD82 pKa = 3.51AKK84 pKa = 11.31VDD86 pKa = 3.89LPDD89 pKa = 3.65VSLSPASDD97 pKa = 3.15SGSEE101 pKa = 4.23NEE103 pKa = 5.53FEE105 pKa = 5.55DD106 pKa = 4.25ADD108 pKa = 4.15TPITEE113 pKa = 4.27MRR115 pKa = 11.84FAPDD119 pKa = 3.02NTNNLEE125 pKa = 4.54AMFQAMNQCQALHH138 pKa = 7.44PDD140 pKa = 3.88PQDD143 pKa = 3.37SFSDD147 pKa = 3.74AEE149 pKa = 3.83EE150 pKa = 5.32DD151 pKa = 3.31IYY153 pKa = 11.51EE154 pKa = 4.54DD155 pKa = 4.26AEE157 pKa = 4.14EE158 pKa = 5.55DD159 pKa = 3.94DD160 pKa = 4.26FEE162 pKa = 6.75HH163 pKa = 7.1YY164 pKa = 10.65DD165 pKa = 3.6VGAGDD170 pKa = 4.46APYY173 pKa = 10.4ILPTGFLFSFYY184 pKa = 10.87NILEE188 pKa = 4.37QIGTNHH194 pKa = 6.66NGTEE198 pKa = 4.34ADD200 pKa = 3.46DD201 pKa = 4.6AMDD204 pKa = 3.71IEE206 pKa = 4.99AGQFEE211 pKa = 4.76DD212 pKa = 5.49AEE214 pKa = 4.39EE215 pKa = 4.16DD216 pKa = 3.59LL217 pKa = 5.34

Molecular weight: 24.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3EPC0|A0A2A3EPC0_APICC Abasic site processing protein HMCES OS=Apis cerana cerana OX=94128 GN=APICC_01750 PE=3 SV=1
MM1 pKa = 7.47SPLNRR6 pKa = 11.84GPLLGPRR13 pKa = 11.84VPPTGVRR20 pKa = 11.84PPPPPRR26 pKa = 11.84FGGRR30 pKa = 11.84LPPPGLPPRR39 pKa = 11.84MPPPPMNPVFGPMRR53 pKa = 11.84QRR55 pKa = 11.84LPPPPRR61 pKa = 11.84PGGVNRR67 pKa = 11.84PSGPMPLFGPRR78 pKa = 11.84VRR80 pKa = 11.84AMAPMVPPMGLRR92 pKa = 11.84GVGPRR97 pKa = 11.84GPLMRR102 pKa = 11.84PWHH105 pKa = 6.23RR106 pKa = 11.84RR107 pKa = 11.84ALPPQILSHH116 pKa = 5.24MRR118 pKa = 11.84PRR120 pKa = 11.84FSIRR124 pKa = 11.84NGNVKK129 pKa = 9.9GKK131 pKa = 10.7AMNNAKK137 pKa = 10.02KK138 pKa = 10.5VSKK141 pKa = 10.99LEE143 pKa = 4.0NNYY146 pKa = 9.76VKK148 pKa = 10.89

Molecular weight: 16.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9931

0

9931

5621110

31

9157

566.0

64.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.481 ± 0.023

1.965 ± 0.019

5.285 ± 0.015

7.072 ± 0.035

3.66 ± 0.019

5.128 ± 0.03

2.425 ± 0.012

6.788 ± 0.028

6.999 ± 0.03

9.108 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.011

5.777 ± 0.024

4.693 ± 0.026

4.344 ± 0.025

5.19 ± 0.021

7.998 ± 0.029

5.892 ± 0.021

5.572 ± 0.016

1.078 ± 0.009

3.273 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski