Streptococcus satellite phage Javan147

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZK54|A0A4D5ZK54_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan147 OX=2558541 GN=JavanS147_0010 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 5.55FSDD5 pKa = 5.66FLNKK9 pKa = 9.27KK10 pKa = 8.17QKK12 pKa = 10.03EE13 pKa = 3.92WDD15 pKa = 3.75EE16 pKa = 3.95FHH18 pKa = 7.54PIPDD22 pKa = 4.1FSMMSDD28 pKa = 3.67EE29 pKa = 4.28EE30 pKa = 4.98LLYY33 pKa = 10.89QPMSEE38 pKa = 4.18ALVSEE43 pKa = 4.67KK44 pKa = 10.12FAKK47 pKa = 10.04EE48 pKa = 3.65LSS50 pKa = 3.62

Molecular weight:
5.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZFS0|A0A4D5ZFS0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan147 OX=2558541 GN=JavanS147_0008 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 9.41YY3 pKa = 8.38TLTGMEE9 pKa = 4.11QLEE12 pKa = 4.61KK13 pKa = 10.46RR14 pKa = 11.84LQSINEE20 pKa = 4.02INWQKK25 pKa = 10.61VANKK29 pKa = 10.21NLTEE33 pKa = 4.05MFNRR37 pKa = 11.84AARR40 pKa = 11.84PPGTPIGKK48 pKa = 7.95NTKK51 pKa = 8.97KK52 pKa = 10.18HH53 pKa = 5.39SAGEE57 pKa = 3.93LMRR60 pKa = 11.84SRR62 pKa = 11.84RR63 pKa = 11.84LNRR66 pKa = 11.84VNISKK71 pKa = 9.03GHH73 pKa = 5.4WLGEE77 pKa = 3.61FGYY80 pKa = 10.18IKK82 pKa = 10.36DD83 pKa = 3.96YY84 pKa = 11.0APHH87 pKa = 6.57VEE89 pKa = 4.05YY90 pKa = 10.65GHH92 pKa = 6.96RR93 pKa = 11.84IVRR96 pKa = 11.84NGKK99 pKa = 8.29QVGYY103 pKa = 11.31VNGTKK108 pKa = 10.65YY109 pKa = 10.86LFNNVKK115 pKa = 9.38KK116 pKa = 10.39QRR118 pKa = 11.84EE119 pKa = 4.35IYY121 pKa = 10.35RR122 pKa = 11.84QDD124 pKa = 3.26LLQAIKK130 pKa = 11.03NNGKK134 pKa = 8.42GTVSSGSSSSLDD146 pKa = 3.49LLKK149 pKa = 9.83TAKK152 pKa = 10.16NLNQTYY158 pKa = 9.95QRR160 pKa = 11.84VYY162 pKa = 10.65SPNGKK167 pKa = 9.72LEE169 pKa = 4.14FKK171 pKa = 10.28PVSKK175 pKa = 10.64SNRR178 pKa = 11.84LISLAKK184 pKa = 9.0KK185 pKa = 8.7TYY187 pKa = 9.8KK188 pKa = 10.45QLDD191 pKa = 3.76DD192 pKa = 3.72

Molecular weight:
22.02 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2176

50

381

155.4

18.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.147 ± 0.566

0.643 ± 0.234

5.101 ± 0.591

10.386 ± 1.401

3.814 ± 0.394

3.493 ± 0.581

1.517 ± 0.262

7.123 ± 0.433

10.754 ± 0.518

10.478 ± 0.397

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.237

6.664 ± 0.804

2.16 ± 0.304

4.825 ± 0.419

4.55 ± 0.614

6.664 ± 0.459

5.055 ± 0.447

4.136 ± 0.682

0.735 ± 0.144

4.55 ± 0.443

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski