Amino acid dipepetide frequency for Pseudomonas phage phiPSA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.452AlaAla: 17.452 ± 2.378
1.236AlaCys: 1.236 ± 0.374
6.908AlaAsp: 6.908 ± 0.8
8.435AlaGlu: 8.435 ± 1.185
3.636AlaPhe: 3.636 ± 0.38
9.526AlaGly: 9.526 ± 1.118
1.6AlaHis: 1.6 ± 0.357
4.727AlaIle: 4.727 ± 0.541
5.526AlaLys: 5.526 ± 0.742
9.38AlaLeu: 9.38 ± 1.119
4.072AlaMet: 4.072 ± 0.421
5.163AlaAsn: 5.163 ± 0.625
3.636AlaPro: 3.636 ± 0.585
6.835AlaGln: 6.835 ± 0.95
6.908AlaArg: 6.908 ± 0.653
7.272AlaSer: 7.272 ± 0.885
5.526AlaThr: 5.526 ± 0.675
7.054AlaVal: 7.054 ± 0.652
2.4AlaTrp: 2.4 ± 0.464
3.927AlaTyr: 3.927 ± 0.571
0.0AlaXaa: 0.0 ± 0.0
Cys
1.018CysAla: 1.018 ± 0.306
0.145CysCys: 0.145 ± 0.119
0.364CysAsp: 0.364 ± 0.16
0.654CysGlu: 0.654 ± 0.188
0.218CysPhe: 0.218 ± 0.146
1.236CysGly: 1.236 ± 0.402
0.436CysHis: 0.436 ± 0.162
0.8CysIle: 0.8 ± 0.263
0.436CysLys: 0.436 ± 0.146
1.382CysLeu: 1.382 ± 0.378
0.145CysMet: 0.145 ± 0.09
0.291CysAsn: 0.291 ± 0.148
0.509CysPro: 0.509 ± 0.237
0.073CysGln: 0.073 ± 0.057
0.873CysArg: 0.873 ± 0.279
0.436CysSer: 0.436 ± 0.207
0.436CysThr: 0.436 ± 0.148
1.091CysVal: 1.091 ± 0.301
0.145CysTrp: 0.145 ± 0.105
0.145CysTyr: 0.145 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
7.272AspAla: 7.272 ± 1.037
0.8AspCys: 0.8 ± 0.319
2.981AspAsp: 2.981 ± 0.557
3.418AspGlu: 3.418 ± 0.547
2.036AspPhe: 2.036 ± 0.358
6.035AspGly: 6.035 ± 0.543
1.018AspHis: 1.018 ± 0.332
2.545AspIle: 2.545 ± 0.369
2.182AspLys: 2.182 ± 0.398
4.29AspLeu: 4.29 ± 0.493
1.091AspMet: 1.091 ± 0.205
1.672AspAsn: 1.672 ± 0.305
2.036AspPro: 2.036 ± 0.339
2.618AspGln: 2.618 ± 0.383
3.49AspArg: 3.49 ± 0.529
4.072AspSer: 4.072 ± 0.601
2.909AspThr: 2.909 ± 0.574
3.563AspVal: 3.563 ± 0.499
1.6AspTrp: 1.6 ± 0.266
1.745AspTyr: 1.745 ± 0.358
0.0AspXaa: 0.0 ± 0.0
Glu
7.563GluAla: 7.563 ± 1.075
0.8GluCys: 0.8 ± 0.26
2.254GluAsp: 2.254 ± 0.396
3.636GluGlu: 3.636 ± 0.549
2.836GluPhe: 2.836 ± 0.372
3.854GluGly: 3.854 ± 0.531
0.727GluHis: 0.727 ± 0.252
3.999GluIle: 3.999 ± 0.749
3.272GluLys: 3.272 ± 0.512
6.326GluLeu: 6.326 ± 0.702
0.727GluMet: 0.727 ± 0.292
1.454GluAsn: 1.454 ± 0.274
2.036GluPro: 2.036 ± 0.436
4.29GluGln: 4.29 ± 0.642
3.854GluArg: 3.854 ± 0.709
2.691GluSer: 2.691 ± 0.365
2.545GluThr: 2.545 ± 0.398
3.999GluVal: 3.999 ± 0.484
1.018GluTrp: 1.018 ± 0.305
1.527GluTyr: 1.527 ± 0.33
0.0GluXaa: 0.0 ± 0.0
Phe
3.636PheAla: 3.636 ± 0.482
0.145PheCys: 0.145 ± 0.097
2.472PheAsp: 2.472 ± 0.405
1.891PheGlu: 1.891 ± 0.365
0.436PhePhe: 0.436 ± 0.211
2.763PheGly: 2.763 ± 0.58
0.509PheHis: 0.509 ± 0.171
2.327PheIle: 2.327 ± 0.436
1.309PheLys: 1.309 ± 0.307
2.472PheLeu: 2.472 ± 0.45
0.945PheMet: 0.945 ± 0.283
1.309PheAsn: 1.309 ± 0.329
1.818PhePro: 1.818 ± 0.35
0.873PheGln: 0.873 ± 0.306
1.382PheArg: 1.382 ± 0.282
2.618PheSer: 2.618 ± 0.472
1.963PheThr: 1.963 ± 0.331
2.545PheVal: 2.545 ± 0.534
0.364PheTrp: 0.364 ± 0.186
1.163PheTyr: 1.163 ± 0.267
0.0PheXaa: 0.0 ± 0.0
Gly
8.144GlyAla: 8.144 ± 1.016
1.163GlyCys: 1.163 ± 0.262
3.709GlyAsp: 3.709 ± 0.435
4.508GlyGlu: 4.508 ± 0.473
2.4GlyPhe: 2.4 ± 0.363
6.835GlyGly: 6.835 ± 0.78
1.745GlyHis: 1.745 ± 0.406
3.49GlyIle: 3.49 ± 0.541
5.308GlyLys: 5.308 ± 0.414
6.108GlyLeu: 6.108 ± 0.748
1.818GlyMet: 1.818 ± 0.455
2.981GlyAsn: 2.981 ± 0.592
2.109GlyPro: 2.109 ± 0.394
4.072GlyGln: 4.072 ± 0.494
5.163GlyArg: 5.163 ± 0.769
5.236GlySer: 5.236 ± 0.62
5.017GlyThr: 5.017 ± 0.737
6.108GlyVal: 6.108 ± 0.557
1.163GlyTrp: 1.163 ± 0.369
2.763GlyTyr: 2.763 ± 0.495
0.0GlyXaa: 0.0 ± 0.0
His
2.036HisAla: 2.036 ± 0.423
0.291HisCys: 0.291 ± 0.147
1.018HisAsp: 1.018 ± 0.294
0.8HisGlu: 0.8 ± 0.195
0.364HisPhe: 0.364 ± 0.15
1.236HisGly: 1.236 ± 0.341
0.218HisHis: 0.218 ± 0.141
1.163HisIle: 1.163 ± 0.267
0.727HisLys: 0.727 ± 0.274
0.8HisLeu: 0.8 ± 0.229
0.436HisMet: 0.436 ± 0.222
0.727HisAsn: 0.727 ± 0.223
0.945HisPro: 0.945 ± 0.293
0.654HisGln: 0.654 ± 0.209
0.582HisArg: 0.582 ± 0.218
1.309HisSer: 1.309 ± 0.35
1.163HisThr: 1.163 ± 0.267
0.727HisVal: 0.727 ± 0.229
0.145HisTrp: 0.145 ± 0.114
0.8HisTyr: 0.8 ± 0.29
0.0HisXaa: 0.0 ± 0.0
Ile
6.545IleAla: 6.545 ± 0.646
0.509IleCys: 0.509 ± 0.224
3.636IleAsp: 3.636 ± 0.514
3.054IleGlu: 3.054 ± 0.516
1.309IlePhe: 1.309 ± 0.339
3.854IleGly: 3.854 ± 0.51
0.873IleHis: 0.873 ± 0.294
2.327IleIle: 2.327 ± 0.389
1.963IleLys: 1.963 ± 0.418
2.763IleLeu: 2.763 ± 0.486
0.8IleMet: 0.8 ± 0.244
2.254IleAsn: 2.254 ± 0.37
2.327IlePro: 2.327 ± 0.42
2.182IleGln: 2.182 ± 0.446
2.691IleArg: 2.691 ± 0.426
3.49IleSer: 3.49 ± 0.584
4.072IleThr: 4.072 ± 0.55
3.563IleVal: 3.563 ± 0.514
0.582IleTrp: 0.582 ± 0.222
1.236IleTyr: 1.236 ± 0.353
0.0IleXaa: 0.0 ± 0.0
Lys
6.617LysAla: 6.617 ± 0.71
0.364LysCys: 0.364 ± 0.173
2.618LysAsp: 2.618 ± 0.355
2.109LysGlu: 2.109 ± 0.483
1.745LysPhe: 1.745 ± 0.369
2.836LysGly: 2.836 ± 0.42
0.654LysHis: 0.654 ± 0.244
2.036LysIle: 2.036 ± 0.34
2.618LysLys: 2.618 ± 0.469
4.363LysLeu: 4.363 ± 0.512
1.454LysMet: 1.454 ± 0.358
1.527LysAsn: 1.527 ± 0.285
2.472LysPro: 2.472 ± 0.348
1.745LysGln: 1.745 ± 0.415
3.272LysArg: 3.272 ± 0.563
3.418LysSer: 3.418 ± 0.558
2.981LysThr: 2.981 ± 0.429
3.563LysVal: 3.563 ± 0.561
0.727LysTrp: 0.727 ± 0.26
1.818LysTyr: 1.818 ± 0.429
0.0LysXaa: 0.0 ± 0.0
Leu
10.326LeuAla: 10.326 ± 1.156
0.436LeuCys: 0.436 ± 0.162
4.436LeuAsp: 4.436 ± 0.482
5.09LeuGlu: 5.09 ± 0.498
2.4LeuPhe: 2.4 ± 0.419
6.181LeuGly: 6.181 ± 0.686
1.309LeuHis: 1.309 ± 0.336
3.345LeuIle: 3.345 ± 0.484
3.563LeuLys: 3.563 ± 0.592
5.963LeuLeu: 5.963 ± 0.63
0.873LeuMet: 0.873 ± 0.222
4.145LeuAsn: 4.145 ± 0.459
4.072LeuPro: 4.072 ± 0.722
2.545LeuGln: 2.545 ± 0.438
4.654LeuArg: 4.654 ± 0.457
5.454LeuSer: 5.454 ± 0.657
4.945LeuThr: 4.945 ± 0.785
4.508LeuVal: 4.508 ± 0.518
0.873LeuTrp: 0.873 ± 0.266
1.454LeuTyr: 1.454 ± 0.386
0.0LeuXaa: 0.0 ± 0.0
Met
2.909MetAla: 2.909 ± 0.449
0.218MetCys: 0.218 ± 0.128
1.236MetAsp: 1.236 ± 0.297
1.163MetGlu: 1.163 ± 0.379
0.509MetPhe: 0.509 ± 0.228
1.963MetGly: 1.963 ± 0.354
0.218MetHis: 0.218 ± 0.173
1.454MetIle: 1.454 ± 0.224
1.382MetLys: 1.382 ± 0.39
1.527MetLeu: 1.527 ± 0.274
0.218MetMet: 0.218 ± 0.122
0.873MetAsn: 0.873 ± 0.242
2.036MetPro: 2.036 ± 0.41
0.873MetGln: 0.873 ± 0.244
1.236MetArg: 1.236 ± 0.4
2.109MetSer: 2.109 ± 0.34
1.6MetThr: 1.6 ± 0.324
1.309MetVal: 1.309 ± 0.288
0.218MetTrp: 0.218 ± 0.119
0.364MetTyr: 0.364 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
4.363AsnAla: 4.363 ± 0.749
0.8AsnCys: 0.8 ± 0.211
2.327AsnAsp: 2.327 ± 0.382
1.963AsnGlu: 1.963 ± 0.441
0.8AsnPhe: 0.8 ± 0.167
3.636AsnGly: 3.636 ± 0.516
0.8AsnHis: 0.8 ± 0.242
1.672AsnIle: 1.672 ± 0.347
1.672AsnLys: 1.672 ± 0.406
2.327AsnLeu: 2.327 ± 0.393
0.291AsnMet: 0.291 ± 0.153
1.745AsnAsn: 1.745 ± 0.628
1.891AsnPro: 1.891 ± 0.41
1.527AsnGln: 1.527 ± 0.411
1.382AsnArg: 1.382 ± 0.309
3.272AsnSer: 3.272 ± 0.607
3.127AsnThr: 3.127 ± 0.594
2.4AsnVal: 2.4 ± 0.413
0.582AsnTrp: 0.582 ± 0.21
0.945AsnTyr: 0.945 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
4.799ProAla: 4.799 ± 0.724
0.509ProCys: 0.509 ± 0.236
2.763ProAsp: 2.763 ± 0.487
2.545ProGlu: 2.545 ± 0.434
1.672ProPhe: 1.672 ± 0.368
3.418ProGly: 3.418 ± 0.473
0.364ProHis: 0.364 ± 0.162
2.327ProIle: 2.327 ± 0.406
1.963ProLys: 1.963 ± 0.378
2.472ProLeu: 2.472 ± 0.432
1.527ProMet: 1.527 ± 0.305
1.6ProAsn: 1.6 ± 0.281
1.454ProPro: 1.454 ± 0.331
1.672ProGln: 1.672 ± 0.355
1.745ProArg: 1.745 ± 0.324
2.691ProSer: 2.691 ± 0.396
2.691ProThr: 2.691 ± 0.435
2.981ProVal: 2.981 ± 0.638
0.364ProTrp: 0.364 ± 0.143
1.382ProTyr: 1.382 ± 0.29
0.0ProXaa: 0.0 ± 0.0
Gln
6.617GlnAla: 6.617 ± 0.985
0.509GlnCys: 0.509 ± 0.206
1.963GlnAsp: 1.963 ± 0.354
2.182GlnGlu: 2.182 ± 0.339
1.818GlnPhe: 1.818 ± 0.33
2.763GlnGly: 2.763 ± 0.378
0.364GlnHis: 0.364 ± 0.16
2.618GlnIle: 2.618 ± 0.5
2.618GlnLys: 2.618 ± 0.648
4.145GlnLeu: 4.145 ± 0.624
0.8GlnMet: 0.8 ± 0.214
1.309GlnAsn: 1.309 ± 0.339
1.963GlnPro: 1.963 ± 0.341
2.182GlnGln: 2.182 ± 0.62
3.054GlnArg: 3.054 ± 0.452
3.636GlnSer: 3.636 ± 0.699
2.254GlnThr: 2.254 ± 0.518
3.418GlnVal: 3.418 ± 0.513
0.654GlnTrp: 0.654 ± 0.199
1.018GlnTyr: 1.018 ± 0.221
0.0GlnXaa: 0.0 ± 0.0
Arg
5.745ArgAla: 5.745 ± 0.527
0.8ArgCys: 0.8 ± 0.218
4.145ArgAsp: 4.145 ± 0.686
3.054ArgGlu: 3.054 ± 0.678
1.963ArgPhe: 1.963 ± 0.378
2.836ArgGly: 2.836 ± 0.388
1.163ArgHis: 1.163 ± 0.37
3.418ArgIle: 3.418 ± 0.396
3.418ArgLys: 3.418 ± 0.702
5.236ArgLeu: 5.236 ± 0.573
2.691ArgMet: 2.691 ± 0.548
2.327ArgAsn: 2.327 ± 0.372
2.472ArgPro: 2.472 ± 0.439
2.909ArgGln: 2.909 ± 0.47
3.927ArgArg: 3.927 ± 0.566
2.981ArgSer: 2.981 ± 0.338
3.054ArgThr: 3.054 ± 0.356
3.854ArgVal: 3.854 ± 0.478
1.236ArgTrp: 1.236 ± 0.287
1.672ArgTyr: 1.672 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
9.162SerAla: 9.162 ± 1.223
0.509SerCys: 0.509 ± 0.206
2.836SerAsp: 2.836 ± 0.455
4.072SerGlu: 4.072 ± 0.524
2.472SerPhe: 2.472 ± 0.388
6.181SerGly: 6.181 ± 0.887
1.454SerHis: 1.454 ± 0.349
3.2SerIle: 3.2 ± 0.426
2.836SerLys: 2.836 ± 0.469
4.872SerLeu: 4.872 ± 0.515
2.036SerMet: 2.036 ± 0.336
2.4SerAsn: 2.4 ± 0.491
1.963SerPro: 1.963 ± 0.308
2.691SerGln: 2.691 ± 0.426
3.49SerArg: 3.49 ± 0.513
4.508SerSer: 4.508 ± 0.835
4.654SerThr: 4.654 ± 0.607
3.927SerVal: 3.927 ± 0.545
1.382SerTrp: 1.382 ± 0.299
2.254SerTyr: 2.254 ± 0.321
0.0SerXaa: 0.0 ± 0.0
Thr
6.908ThrAla: 6.908 ± 0.88
0.291ThrCys: 0.291 ± 0.156
3.927ThrAsp: 3.927 ± 0.53
3.999ThrGlu: 3.999 ± 0.534
2.254ThrPhe: 2.254 ± 0.396
6.326ThrGly: 6.326 ± 0.61
1.018ThrHis: 1.018 ± 0.369
2.691ThrIle: 2.691 ± 0.398
2.909ThrLys: 2.909 ± 0.586
4.508ThrLeu: 4.508 ± 0.563
1.018ThrMet: 1.018 ± 0.281
2.109ThrAsn: 2.109 ± 0.412
2.763ThrPro: 2.763 ± 0.453
2.545ThrGln: 2.545 ± 0.421
3.2ThrArg: 3.2 ± 0.525
3.927ThrSer: 3.927 ± 0.476
3.49ThrThr: 3.49 ± 0.547
4.363ThrVal: 4.363 ± 0.489
0.873ThrTrp: 0.873 ± 0.286
1.6ThrTyr: 1.6 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
6.472ValAla: 6.472 ± 0.649
1.018ValCys: 1.018 ± 0.298
3.927ValAsp: 3.927 ± 0.552
4.363ValGlu: 4.363 ± 0.684
2.618ValPhe: 2.618 ± 0.359
4.872ValGly: 4.872 ± 0.565
1.018ValHis: 1.018 ± 0.286
3.781ValIle: 3.781 ± 0.515
3.127ValLys: 3.127 ± 0.511
3.999ValLeu: 3.999 ± 0.52
1.6ValMet: 1.6 ± 0.281
2.909ValAsn: 2.909 ± 0.424
2.618ValPro: 2.618 ± 0.444
2.836ValGln: 2.836 ± 0.516
4.29ValArg: 4.29 ± 0.645
4.945ValSer: 4.945 ± 0.501
5.526ValThr: 5.526 ± 0.58
5.672ValVal: 5.672 ± 0.672
0.654ValTrp: 0.654 ± 0.19
1.6ValTyr: 1.6 ± 0.394
0.0ValXaa: 0.0 ± 0.0
Trp
1.6TrpAla: 1.6 ± 0.323
0.073TrpCys: 0.073 ± 0.077
1.309TrpAsp: 1.309 ± 0.383
0.945TrpGlu: 0.945 ± 0.305
0.8TrpPhe: 0.8 ± 0.211
1.091TrpGly: 1.091 ± 0.325
0.218TrpHis: 0.218 ± 0.111
0.436TrpIle: 0.436 ± 0.165
1.091TrpLys: 1.091 ± 0.324
1.163TrpLeu: 1.163 ± 0.34
0.291TrpMet: 0.291 ± 0.124
0.145TrpAsn: 0.145 ± 0.123
0.727TrpPro: 0.727 ± 0.229
1.018TrpGln: 1.018 ± 0.265
1.236TrpArg: 1.236 ± 0.301
0.945TrpSer: 0.945 ± 0.25
1.091TrpThr: 1.091 ± 0.277
0.8TrpVal: 0.8 ± 0.342
0.218TrpTrp: 0.218 ± 0.135
0.582TrpTyr: 0.582 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.036TyrAla: 2.036 ± 0.453
0.218TyrCys: 0.218 ± 0.13
2.545TyrAsp: 2.545 ± 0.444
1.527TyrGlu: 1.527 ± 0.391
0.654TyrPhe: 0.654 ± 0.214
2.472TyrGly: 2.472 ± 0.453
0.436TyrHis: 0.436 ± 0.181
1.672TyrIle: 1.672 ± 0.293
1.018TyrLys: 1.018 ± 0.258
2.472TyrLeu: 2.472 ± 0.352
0.509TyrMet: 0.509 ± 0.222
0.582TyrAsn: 0.582 ± 0.263
1.091TyrPro: 1.091 ± 0.336
1.745TyrGln: 1.745 ± 0.326
2.472TyrArg: 2.472 ± 0.47
1.891TyrSer: 1.891 ± 0.359
1.672TyrThr: 1.672 ± 0.338
2.4TyrVal: 2.4 ± 0.402
0.582TyrTrp: 0.582 ± 0.222
0.8TyrTyr: 0.8 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (13753 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski