Microbacterium sp. CGR2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B0BAN9|A0A3B0BAN9_9MICO Transcription termination factor Rho OS=Microbacterium sp. CGR2 OX=1805820 GN=rho PE=3 SV=1
MM1 pKa = 7.69PRR3 pKa = 11.84RR4 pKa = 11.84NLFAGLALAATATLALSGCATGGSDD29 pKa = 4.87DD30 pKa = 4.21TDD32 pKa = 3.91AGAPAAGEE40 pKa = 4.12DD41 pKa = 3.58YY42 pKa = 11.51GLIEE46 pKa = 5.91DD47 pKa = 4.39GTLTVCSDD55 pKa = 2.68IPYY58 pKa = 10.6APFEE62 pKa = 4.33FEE64 pKa = 4.81GGDD67 pKa = 3.25NGTGYY72 pKa = 9.24TGFDD76 pKa = 3.56IDD78 pKa = 5.52LLDD81 pKa = 5.62AIAKK85 pKa = 10.24KK86 pKa = 10.58LDD88 pKa = 3.55LKK90 pKa = 11.21LAVQDD95 pKa = 3.78VAFEE99 pKa = 4.3ALQSGTTLASGQCDD113 pKa = 2.83IGASAMTITEE123 pKa = 3.98EE124 pKa = 3.86RR125 pKa = 11.84KK126 pKa = 10.48ANIDD130 pKa = 3.5FSDD133 pKa = 4.32PYY135 pKa = 11.01YY136 pKa = 11.11DD137 pKa = 3.78SLQSLLVGTDD147 pKa = 3.3SGIEE151 pKa = 4.19SIDD154 pKa = 3.82DD155 pKa = 3.97LDD157 pKa = 4.1GKK159 pKa = 10.82AVGVQQGTTGEE170 pKa = 4.23AYY172 pKa = 10.44ANEE175 pKa = 4.15NAEE178 pKa = 3.99GAEE181 pKa = 4.0IVQYY185 pKa = 10.78PSDD188 pKa = 4.19GEE190 pKa = 4.12LWPAMQSGLIDD201 pKa = 6.3AILQDD206 pKa = 3.45QPVNLEE212 pKa = 4.0HH213 pKa = 7.04EE214 pKa = 4.62KK215 pKa = 11.13ADD217 pKa = 3.56DD218 pKa = 3.83AYY220 pKa = 10.78AIVEE224 pKa = 4.25EE225 pKa = 4.76YY226 pKa = 8.21EE227 pKa = 4.05TGEE230 pKa = 4.18SYY232 pKa = 11.4GFAFAKK238 pKa = 10.45GEE240 pKa = 3.97RR241 pKa = 11.84DD242 pKa = 3.48EE243 pKa = 5.57LLEE246 pKa = 4.24AVNGALAEE254 pKa = 4.41LKK256 pKa = 10.82DD257 pKa = 3.56SGDD260 pKa = 3.38YY261 pKa = 8.96KK262 pKa = 10.68TIYY265 pKa = 9.15DD266 pKa = 3.83TYY268 pKa = 9.68FTAKK272 pKa = 10.3

Molecular weight: 28.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B0B3X4|A0A3B0B3X4_9MICO Uncharacterized protein OS=Microbacterium sp. CGR2 OX=1805820 GN=D7252_07510 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3832

0

3832

1195693

29

2022

312.0

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.29 ± 0.053

0.472 ± 0.009

6.291 ± 0.037

5.844 ± 0.039

3.186 ± 0.024

8.762 ± 0.032

2.023 ± 0.02

4.821 ± 0.028

1.974 ± 0.03

10.026 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.884 ± 0.017

2.008 ± 0.023

5.235 ± 0.028

2.818 ± 0.019

7.263 ± 0.047

5.747 ± 0.026

6.109 ± 0.032

8.778 ± 0.038

1.541 ± 0.018

1.93 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski