Asparagus officinalis (Garden asparagus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; Asparagales; Asparagaceae; Asparagoideae; Asparagus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P1EZZ2|A0A5P1EZZ2_ASPOF ADH_N domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C04F4220 PE=4 SV=1
MM1 pKa = 7.03YY2 pKa = 10.48VSLMNLYY9 pKa = 10.54GEE11 pKa = 4.46YY12 pKa = 10.09EE13 pKa = 4.0AAVDD17 pKa = 4.1GDD19 pKa = 4.28VACKK23 pKa = 9.93QDD25 pKa = 3.78WISDD29 pKa = 3.45SDD31 pKa = 4.4GNDD34 pKa = 3.27DD35 pKa = 4.32YY36 pKa = 12.15DD37 pKa = 3.76WGTHH41 pKa = 5.26EE42 pKa = 5.5ADD44 pKa = 4.79LVPASDD50 pKa = 4.26GSAGPSKK57 pKa = 10.29KK58 pKa = 10.25AKK60 pKa = 9.81PSGGEE65 pKa = 3.99EE66 pKa = 3.96NHH68 pKa = 7.46DD69 pKa = 4.24SGDD72 pKa = 3.68DD73 pKa = 3.88SGWDD77 pKa = 3.39TDD79 pKa = 3.6EE80 pKa = 4.61GAVRR84 pKa = 11.84AHH86 pKa = 6.59GAVSAPSPLEE96 pKa = 3.76EE97 pKa = 4.01EE98 pKa = 4.75LYY100 pKa = 11.06EE101 pKa = 4.68GGSS104 pKa = 3.42

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P1FL25|A0A5P1FL25_ASPOF Uncharacterized protein OS=Asparagus officinalis OX=4686 GN=A4U43_C01F2110 PE=4 SV=1
MM1 pKa = 7.53LRR3 pKa = 11.84PALSRR8 pKa = 11.84AAPAAARR15 pKa = 11.84RR16 pKa = 11.84PWPLARR22 pKa = 11.84PPPPAAVANRR32 pKa = 11.84HH33 pKa = 4.85RR34 pKa = 11.84HH35 pKa = 4.68ASAAAGRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84PRR46 pKa = 11.84VPVRR50 pKa = 11.84ATPLAARR57 pKa = 11.84RR58 pKa = 11.84LPSPSAARR66 pKa = 11.84NHH68 pKa = 6.39RR69 pKa = 11.84PSLPAAPLWCRR80 pKa = 11.84SAPVDD85 pKa = 3.59TTGSSVGRR93 pKa = 11.84FRR95 pKa = 11.84KK96 pKa = 9.89AIRR99 pKa = 11.84RR100 pKa = 11.84LRR102 pKa = 11.84APARR106 pKa = 11.84GALSPAGSPPLVVPSRR122 pKa = 11.84RR123 pKa = 11.84WFRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84AAALPPPPLYY141 pKa = 10.45KK142 pKa = 10.62

Molecular weight: 15.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24059

0

24059

8059799

50

5170

335.0

37.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.01 ± 0.015

1.799 ± 0.008

5.345 ± 0.011

6.786 ± 0.018

3.954 ± 0.012

6.803 ± 0.017

2.318 ± 0.007

5.186 ± 0.011

5.922 ± 0.016

9.563 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.475 ± 0.007

4.104 ± 0.01

5.028 ± 0.015

3.526 ± 0.012

5.957 ± 0.015

9.05 ± 0.023

4.678 ± 0.011

6.485 ± 0.012

1.285 ± 0.007

2.653 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski