Amino acid dipepetide frequency for Clostridium phage CDKM15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.761AlaAla: 1.761 ± 0.429
0.339AlaCys: 0.339 ± 0.153
2.641AlaAsp: 2.641 ± 0.428
3.928AlaGlu: 3.928 ± 0.591
1.625AlaPhe: 1.625 ± 0.403
2.776AlaGly: 2.776 ± 0.567
0.339AlaHis: 0.339 ± 0.133
4.469AlaIle: 4.469 ± 0.607
4.943AlaLys: 4.943 ± 0.557
5.824AlaLeu: 5.824 ± 0.736
1.558AlaMet: 1.558 ± 0.293
2.776AlaAsn: 2.776 ± 0.394
0.813AlaPro: 0.813 ± 0.214
1.354AlaGln: 1.354 ± 0.271
2.032AlaArg: 2.032 ± 0.426
3.928AlaSer: 3.928 ± 0.636
3.86AlaThr: 3.86 ± 0.605
2.844AlaVal: 2.844 ± 0.409
0.271AlaTrp: 0.271 ± 0.12
1.896AlaTyr: 1.896 ± 0.365
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.129
0.271CysCys: 0.271 ± 0.132
0.677CysAsp: 0.677 ± 0.233
0.813CysGlu: 0.813 ± 0.265
0.339CysPhe: 0.339 ± 0.184
0.474CysGly: 0.474 ± 0.166
0.135CysHis: 0.135 ± 0.093
1.354CysIle: 1.354 ± 0.279
1.016CysLys: 1.016 ± 0.259
0.948CysLeu: 0.948 ± 0.211
0.339CysMet: 0.339 ± 0.179
0.339CysAsn: 0.339 ± 0.14
0.068CysPro: 0.068 ± 0.057
0.135CysGln: 0.135 ± 0.087
0.609CysArg: 0.609 ± 0.195
0.271CysSer: 0.271 ± 0.146
0.474CysThr: 0.474 ± 0.148
0.609CysVal: 0.609 ± 0.186
0.203CysTrp: 0.203 ± 0.115
0.542CysTyr: 0.542 ± 0.212
0.0CysXaa: 0.0 ± 0.0
Asp
2.776AspAla: 2.776 ± 0.357
0.88AspCys: 0.88 ± 0.262
3.318AspAsp: 3.318 ± 0.626
5.892AspGlu: 5.892 ± 0.683
2.573AspPhe: 2.573 ± 0.396
3.657AspGly: 3.657 ± 0.527
0.135AspHis: 0.135 ± 0.089
5.485AspIle: 5.485 ± 0.545
7.178AspLys: 7.178 ± 0.887
3.995AspLeu: 3.995 ± 0.427
1.828AspMet: 1.828 ± 0.358
4.537AspAsn: 4.537 ± 0.616
0.88AspPro: 0.88 ± 0.276
0.406AspGln: 0.406 ± 0.205
1.896AspArg: 1.896 ± 0.394
3.657AspSer: 3.657 ± 0.549
2.912AspThr: 2.912 ± 0.426
2.844AspVal: 2.844 ± 0.415
0.406AspTrp: 0.406 ± 0.164
2.302AspTyr: 2.302 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
5.011GluAla: 5.011 ± 0.527
0.88GluCys: 0.88 ± 0.291
4.74GluAsp: 4.74 ± 0.649
7.788GluGlu: 7.788 ± 0.816
4.131GluPhe: 4.131 ± 0.559
3.792GluGly: 3.792 ± 0.502
0.609GluHis: 0.609 ± 0.215
8.194GluIle: 8.194 ± 0.866
9.819GluLys: 9.819 ± 1.029
9.345GluLeu: 9.345 ± 0.852
3.25GluMet: 3.25 ± 0.55
6.975GluAsn: 6.975 ± 0.724
1.083GluPro: 1.083 ± 0.236
2.573GluGln: 2.573 ± 0.401
2.912GluArg: 2.912 ± 0.552
3.521GluSer: 3.521 ± 0.459
4.605GluThr: 4.605 ± 0.682
5.214GluVal: 5.214 ± 0.662
0.677GluTrp: 0.677 ± 0.25
3.86GluTyr: 3.86 ± 0.605
0.0GluXaa: 0.0 ± 0.0
Phe
1.354PheAla: 1.354 ± 0.399
0.135PheCys: 0.135 ± 0.099
2.235PheAsp: 2.235 ± 0.351
3.589PheGlu: 3.589 ± 0.487
1.151PhePhe: 1.151 ± 0.262
2.506PheGly: 2.506 ± 0.396
0.542PheHis: 0.542 ± 0.187
4.808PheIle: 4.808 ± 0.619
4.199PheLys: 4.199 ± 0.483
3.25PheLeu: 3.25 ± 0.443
0.745PheMet: 0.745 ± 0.227
3.318PheAsn: 3.318 ± 0.457
1.016PhePro: 1.016 ± 0.306
1.016PheGln: 1.016 ± 0.238
1.422PheArg: 1.422 ± 0.375
2.302PheSer: 2.302 ± 0.379
1.828PheThr: 1.828 ± 0.393
2.032PheVal: 2.032 ± 0.464
0.203PheTrp: 0.203 ± 0.104
1.49PheTyr: 1.49 ± 0.357
0.0PheXaa: 0.0 ± 0.0
Gly
2.844GlyAla: 2.844 ± 0.574
0.677GlyCys: 0.677 ± 0.22
2.302GlyAsp: 2.302 ± 0.404
5.621GlyGlu: 5.621 ± 0.696
3.318GlyPhe: 3.318 ± 0.516
3.792GlyGly: 3.792 ± 0.751
0.88GlyHis: 0.88 ± 0.267
4.808GlyIle: 4.808 ± 0.793
4.605GlyLys: 4.605 ± 0.526
4.199GlyLeu: 4.199 ± 0.489
2.167GlyMet: 2.167 ± 0.383
3.318GlyAsn: 3.318 ± 0.517
0.406GlyPro: 0.406 ± 0.207
1.49GlyGln: 1.49 ± 0.319
1.625GlyArg: 1.625 ± 0.293
3.792GlySer: 3.792 ± 0.512
3.454GlyThr: 3.454 ± 0.639
3.995GlyVal: 3.995 ± 0.615
0.745GlyTrp: 0.745 ± 0.186
2.032GlyTyr: 2.032 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
0.203HisAla: 0.203 ± 0.111
0.406HisCys: 0.406 ± 0.15
0.271HisAsp: 0.271 ± 0.135
0.677HisGlu: 0.677 ± 0.257
0.745HisPhe: 0.745 ± 0.208
0.339HisGly: 0.339 ± 0.164
0.0HisHis: 0.0 ± 0.0
0.474HisIle: 0.474 ± 0.194
1.151HisLys: 1.151 ± 0.333
0.745HisLeu: 0.745 ± 0.227
0.406HisMet: 0.406 ± 0.182
0.474HisAsn: 0.474 ± 0.15
0.474HisPro: 0.474 ± 0.203
0.271HisGln: 0.271 ± 0.132
0.474HisArg: 0.474 ± 0.194
0.88HisSer: 0.88 ± 0.198
0.609HisThr: 0.609 ± 0.223
0.406HisVal: 0.406 ± 0.166
0.135HisTrp: 0.135 ± 0.075
0.406HisTyr: 0.406 ± 0.172
0.0HisXaa: 0.0 ± 0.0
Ile
4.673IleAla: 4.673 ± 0.594
1.219IleCys: 1.219 ± 0.344
6.907IleAsp: 6.907 ± 0.929
8.262IleGlu: 8.262 ± 0.788
2.98IlePhe: 2.98 ± 0.419
5.079IleGly: 5.079 ± 0.65
0.948IleHis: 0.948 ± 0.262
6.636IleIle: 6.636 ± 0.857
10.632IleLys: 10.632 ± 1.049
7.381IleLeu: 7.381 ± 0.808
1.625IleMet: 1.625 ± 0.362
6.027IleAsn: 6.027 ± 0.619
1.828IlePro: 1.828 ± 0.336
2.776IleGln: 2.776 ± 0.422
3.454IleArg: 3.454 ± 0.54
6.433IleSer: 6.433 ± 0.945
5.079IleThr: 5.079 ± 0.661
5.011IleVal: 5.011 ± 0.666
0.745IleTrp: 0.745 ± 0.277
3.657IleTyr: 3.657 ± 0.506
0.0IleXaa: 0.0 ± 0.0
Lys
5.824LysAla: 5.824 ± 0.785
0.609LysCys: 0.609 ± 0.203
6.636LysAsp: 6.636 ± 0.676
12.189LysGlu: 12.189 ± 0.966
3.25LysPhe: 3.25 ± 0.461
6.162LysGly: 6.162 ± 0.481
1.016LysHis: 1.016 ± 0.291
8.939LysIle: 8.939 ± 0.832
11.106LysLys: 11.106 ± 1.026
7.788LysLeu: 7.788 ± 0.813
2.98LysMet: 2.98 ± 0.478
8.059LysAsn: 8.059 ± 0.775
2.573LysPro: 2.573 ± 0.492
3.928LysGln: 3.928 ± 0.455
4.334LysArg: 4.334 ± 0.498
6.84LysSer: 6.84 ± 0.631
5.824LysThr: 5.824 ± 0.555
7.72LysVal: 7.72 ± 0.587
1.151LysTrp: 1.151 ± 0.253
4.537LysTyr: 4.537 ± 0.551
0.0LysXaa: 0.0 ± 0.0
Leu
4.131LeuAla: 4.131 ± 0.566
1.016LeuCys: 1.016 ± 0.242
5.824LeuAsp: 5.824 ± 0.6
7.788LeuGlu: 7.788 ± 0.841
2.912LeuPhe: 2.912 ± 0.434
5.417LeuGly: 5.417 ± 0.875
0.677LeuHis: 0.677 ± 0.217
6.704LeuIle: 6.704 ± 0.855
12.054LeuLys: 12.054 ± 1.064
7.043LeuLeu: 7.043 ± 0.943
1.422LeuMet: 1.422 ± 0.276
5.892LeuAsn: 5.892 ± 0.643
1.287LeuPro: 1.287 ± 0.311
2.573LeuGln: 2.573 ± 0.409
3.386LeuArg: 3.386 ± 0.421
4.943LeuSer: 4.943 ± 0.652
5.688LeuThr: 5.688 ± 0.64
4.402LeuVal: 4.402 ± 0.519
0.542LeuTrp: 0.542 ± 0.188
2.912LeuTyr: 2.912 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
1.49MetAla: 1.49 ± 0.279
0.203MetCys: 0.203 ± 0.121
1.287MetAsp: 1.287 ± 0.261
1.964MetGlu: 1.964 ± 0.341
0.542MetPhe: 0.542 ± 0.164
0.948MetGly: 0.948 ± 0.249
0.135MetHis: 0.135 ± 0.087
1.761MetIle: 1.761 ± 0.364
3.115MetLys: 3.115 ± 0.345
2.438MetLeu: 2.438 ± 0.408
0.271MetMet: 0.271 ± 0.166
1.422MetAsn: 1.422 ± 0.296
0.609MetPro: 0.609 ± 0.163
0.609MetGln: 0.609 ± 0.185
0.948MetArg: 0.948 ± 0.235
1.693MetSer: 1.693 ± 0.365
1.287MetThr: 1.287 ± 0.277
0.948MetVal: 0.948 ± 0.247
0.339MetTrp: 0.339 ± 0.135
1.219MetTyr: 1.219 ± 0.271
0.0MetXaa: 0.0 ± 0.0
Asn
3.995AsnAla: 3.995 ± 0.573
0.609AsnCys: 0.609 ± 0.195
3.454AsnAsp: 3.454 ± 0.552
4.605AsnGlu: 4.605 ± 0.579
3.454AsnPhe: 3.454 ± 0.508
4.876AsnGly: 4.876 ± 0.479
0.406AsnHis: 0.406 ± 0.173
7.652AsnIle: 7.652 ± 0.6
7.923AsnLys: 7.923 ± 0.829
5.688AsnLeu: 5.688 ± 0.465
1.219AsnMet: 1.219 ± 0.282
4.402AsnAsn: 4.402 ± 0.618
1.761AsnPro: 1.761 ± 0.333
1.016AsnGln: 1.016 ± 0.23
2.98AsnArg: 2.98 ± 0.447
3.928AsnSer: 3.928 ± 0.465
3.25AsnThr: 3.25 ± 0.497
4.266AsnVal: 4.266 ± 0.528
0.948AsnTrp: 0.948 ± 0.293
1.49AsnTyr: 1.49 ± 0.295
0.0AsnXaa: 0.0 ± 0.0
Pro
1.016ProAla: 1.016 ± 0.257
0.339ProCys: 0.339 ± 0.13
0.745ProAsp: 0.745 ± 0.251
1.083ProGlu: 1.083 ± 0.267
0.813ProPhe: 0.813 ± 0.191
0.677ProGly: 0.677 ± 0.166
0.406ProHis: 0.406 ± 0.157
2.573ProIle: 2.573 ± 0.472
2.37ProLys: 2.37 ± 0.429
1.693ProLeu: 1.693 ± 0.291
0.271ProMet: 0.271 ± 0.109
1.083ProAsn: 1.083 ± 0.245
0.135ProPro: 0.135 ± 0.089
0.948ProGln: 0.948 ± 0.199
0.813ProArg: 0.813 ± 0.269
0.88ProSer: 0.88 ± 0.263
1.828ProThr: 1.828 ± 0.341
1.083ProVal: 1.083 ± 0.259
0.068ProTrp: 0.068 ± 0.075
0.745ProTyr: 0.745 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
2.032GlnAla: 2.032 ± 0.415
0.203GlnCys: 0.203 ± 0.116
1.693GlnAsp: 1.693 ± 0.35
2.641GlnGlu: 2.641 ± 0.356
1.016GlnPhe: 1.016 ± 0.251
1.761GlnGly: 1.761 ± 0.321
0.203GlnHis: 0.203 ± 0.143
2.506GlnIle: 2.506 ± 0.415
2.573GlnLys: 2.573 ± 0.473
2.573GlnLeu: 2.573 ± 0.472
0.609GlnMet: 0.609 ± 0.162
2.235GlnAsn: 2.235 ± 0.332
0.474GlnPro: 0.474 ± 0.196
0.88GlnGln: 0.88 ± 0.269
0.609GlnArg: 0.609 ± 0.235
1.354GlnSer: 1.354 ± 0.388
1.828GlnThr: 1.828 ± 0.3
1.287GlnVal: 1.287 ± 0.352
0.203GlnTrp: 0.203 ± 0.112
1.016GlnTyr: 1.016 ± 0.268
0.0GlnXaa: 0.0 ± 0.0
Arg
1.761ArgAla: 1.761 ± 0.29
0.203ArgCys: 0.203 ± 0.137
1.693ArgAsp: 1.693 ± 0.356
4.537ArgGlu: 4.537 ± 0.561
1.422ArgPhe: 1.422 ± 0.353
1.558ArgGly: 1.558 ± 0.342
0.339ArgHis: 0.339 ± 0.135
3.589ArgIle: 3.589 ± 0.478
3.792ArgLys: 3.792 ± 0.641
3.657ArgLeu: 3.657 ± 0.581
1.083ArgMet: 1.083 ± 0.247
1.558ArgAsn: 1.558 ± 0.296
0.948ArgPro: 0.948 ± 0.312
0.813ArgGln: 0.813 ± 0.247
1.422ArgArg: 1.422 ± 0.356
1.625ArgSer: 1.625 ± 0.296
2.032ArgThr: 2.032 ± 0.282
2.641ArgVal: 2.641 ± 0.376
0.474ArgTrp: 0.474 ± 0.159
0.813ArgTyr: 0.813 ± 0.177
0.0ArgXaa: 0.0 ± 0.0
Ser
2.438SerAla: 2.438 ± 0.487
0.474SerCys: 0.474 ± 0.183
3.657SerAsp: 3.657 ± 0.508
4.334SerGlu: 4.334 ± 0.46
2.438SerPhe: 2.438 ± 0.369
3.25SerGly: 3.25 ± 0.732
0.745SerHis: 0.745 ± 0.304
6.095SerIle: 6.095 ± 0.793
6.907SerLys: 6.907 ± 0.705
4.943SerLeu: 4.943 ± 0.769
1.287SerMet: 1.287 ± 0.226
5.282SerAsn: 5.282 ± 0.658
1.083SerPro: 1.083 ± 0.251
2.167SerGln: 2.167 ± 0.342
1.625SerArg: 1.625 ± 0.326
5.35SerSer: 5.35 ± 0.835
3.521SerThr: 3.521 ± 0.482
3.386SerVal: 3.386 ± 0.478
0.406SerTrp: 0.406 ± 0.151
2.37SerTyr: 2.37 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
3.454ThrAla: 3.454 ± 0.434
0.406ThrCys: 0.406 ± 0.24
3.047ThrAsp: 3.047 ± 0.54
3.657ThrGlu: 3.657 ± 0.518
2.573ThrPhe: 2.573 ± 0.367
2.912ThrGly: 2.912 ± 0.633
1.016ThrHis: 1.016 ± 0.214
6.366ThrIle: 6.366 ± 0.471
6.907ThrLys: 6.907 ± 0.648
5.282ThrLeu: 5.282 ± 0.56
1.083ThrMet: 1.083 ± 0.232
3.115ThrAsn: 3.115 ± 0.385
1.964ThrPro: 1.964 ± 0.378
1.625ThrGln: 1.625 ± 0.341
1.828ThrArg: 1.828 ± 0.344
3.725ThrSer: 3.725 ± 0.641
3.115ThrThr: 3.115 ± 0.54
3.86ThrVal: 3.86 ± 0.507
0.406ThrTrp: 0.406 ± 0.152
1.761ThrTyr: 1.761 ± 0.369
0.0ThrXaa: 0.0 ± 0.0
Val
3.183ValAla: 3.183 ± 0.394
0.474ValCys: 0.474 ± 0.165
3.657ValAsp: 3.657 ± 0.498
4.943ValGlu: 4.943 ± 0.504
1.761ValPhe: 1.761 ± 0.306
3.725ValGly: 3.725 ± 0.556
0.406ValHis: 0.406 ± 0.157
4.673ValIle: 4.673 ± 0.729
6.23ValLys: 6.23 ± 0.625
5.553ValLeu: 5.553 ± 0.53
0.542ValMet: 0.542 ± 0.165
4.334ValAsn: 4.334 ± 0.587
0.948ValPro: 0.948 ± 0.214
1.558ValGln: 1.558 ± 0.343
2.37ValArg: 2.37 ± 0.368
4.131ValSer: 4.131 ± 0.442
3.725ValThr: 3.725 ± 0.497
4.199ValVal: 4.199 ± 0.601
0.677ValTrp: 0.677 ± 0.256
1.964ValTyr: 1.964 ± 0.337
0.0ValXaa: 0.0 ± 0.0
Trp
0.474TrpAla: 0.474 ± 0.185
0.135TrpCys: 0.135 ± 0.087
0.745TrpAsp: 0.745 ± 0.212
0.745TrpGlu: 0.745 ± 0.204
0.339TrpPhe: 0.339 ± 0.167
0.474TrpGly: 0.474 ± 0.172
0.068TrpHis: 0.068 ± 0.066
1.083TrpIle: 1.083 ± 0.253
0.542TrpLys: 0.542 ± 0.178
0.88TrpLeu: 0.88 ± 0.229
0.068TrpMet: 0.068 ± 0.065
0.609TrpAsn: 0.609 ± 0.182
0.203TrpPro: 0.203 ± 0.108
0.542TrpGln: 0.542 ± 0.165
0.271TrpArg: 0.271 ± 0.139
0.203TrpSer: 0.203 ± 0.105
0.474TrpThr: 0.474 ± 0.164
0.745TrpVal: 0.745 ± 0.208
0.203TrpTrp: 0.203 ± 0.131
0.406TrpTyr: 0.406 ± 0.244
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.219TyrAla: 1.219 ± 0.3
0.474TyrCys: 0.474 ± 0.171
2.032TyrAsp: 2.032 ± 0.393
3.928TyrGlu: 3.928 ± 0.529
1.761TyrPhe: 1.761 ± 0.377
1.896TyrGly: 1.896 ± 0.374
0.542TyrHis: 0.542 ± 0.192
3.183TyrIle: 3.183 ± 0.501
4.469TyrLys: 4.469 ± 0.681
3.386TyrLeu: 3.386 ± 0.517
0.406TyrMet: 0.406 ± 0.183
2.167TyrAsn: 2.167 ± 0.348
1.016TyrPro: 1.016 ± 0.263
1.016TyrGln: 1.016 ± 0.262
0.948TyrArg: 0.948 ± 0.21
2.302TyrSer: 2.302 ± 0.333
2.709TyrThr: 2.709 ± 0.549
1.558TyrVal: 1.558 ± 0.313
0.406TyrTrp: 0.406 ± 0.195
1.422TyrTyr: 1.422 ± 0.358
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (14768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski