Mycobacterium phage MarkPhew

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SWN4|A0A6M3SWN4_9CAUD Uncharacterized protein OS=Mycobacterium phage MarkPhew OX=2725625 GN=86 PE=4 SV=1
MM1 pKa = 7.63GEE3 pKa = 4.29GGALDD8 pKa = 4.22AGVLGHH14 pKa = 6.3VADD17 pKa = 5.05GVARR21 pKa = 11.84LYY23 pKa = 10.5TGVRR27 pKa = 11.84YY28 pKa = 10.26VYY30 pKa = 10.94VPVAEE35 pKa = 4.7LRR37 pKa = 11.84LADD40 pKa = 3.84EE41 pKa = 4.58VTVEE45 pKa = 3.97EE46 pKa = 4.5WLGLAA51 pKa = 4.87

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SXA7|A0A6M3SXA7_9CAUD 3'-phosphate/5'-hydroxy nucleic acid ligase OS=Mycobacterium phage MarkPhew OX=2725625 GN=87 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.14RR3 pKa = 11.84TRR5 pKa = 11.84AYY7 pKa = 9.57RR8 pKa = 11.84APEE11 pKa = 3.79LAVEE15 pKa = 4.12RR16 pKa = 11.84PEE18 pKa = 4.51VIVHH22 pKa = 5.77GRR24 pKa = 11.84VLEE27 pKa = 4.52PGTEE31 pKa = 3.84VSIAGEE37 pKa = 3.73RR38 pKa = 11.84GRR40 pKa = 11.84FRR42 pKa = 11.84FVKK45 pKa = 10.03SARR48 pKa = 11.84TSSGRR53 pKa = 11.84VTCDD57 pKa = 4.2FIGPDD62 pKa = 4.12DD63 pKa = 3.76QTKK66 pKa = 9.74CWRR69 pKa = 11.84SFYY72 pKa = 9.91PEE74 pKa = 4.32RR75 pKa = 11.84IKK77 pKa = 9.98TVHH80 pKa = 6.33RR81 pKa = 11.84LNRR84 pKa = 11.84TRR86 pKa = 11.84ANAAA90 pKa = 3.2

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19323

40

1357

189.4

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.471 ± 0.443

1.175 ± 0.159

6.433 ± 0.247

5.563 ± 0.327

2.691 ± 0.157

9.186 ± 0.508

2.272 ± 0.172

3.726 ± 0.156

3.079 ± 0.199

8.239 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.118

2.551 ± 0.155

5.755 ± 0.249

3.121 ± 0.121

7.054 ± 0.412

5.191 ± 0.166

5.77 ± 0.191

7.949 ± 0.27

2.08 ± 0.104

2.484 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski