Clostridium phage PhiS63

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2E8V9|I2E8V9_9CAUD Gp11 OS=Clostridium phage PhiS63 OX=1187894 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.39FDD4 pKa = 4.67IIALVADD11 pKa = 4.44SLSSLDD17 pKa = 3.38IPVFEE22 pKa = 4.16GWYY25 pKa = 10.25DD26 pKa = 3.7EE27 pKa = 4.35EE28 pKa = 4.58LKK30 pKa = 9.53KK31 pKa = 8.88THH33 pKa = 5.72ITFFEE38 pKa = 4.28YY39 pKa = 11.01LEE41 pKa = 4.44APEE44 pKa = 5.73DD45 pKa = 3.85FLDD48 pKa = 4.46DD49 pKa = 3.95EE50 pKa = 4.95EE51 pKa = 5.62VSITHH56 pKa = 7.09NIQVDD61 pKa = 2.86IWTTNSEE68 pKa = 4.14EE69 pKa = 4.2GLKK72 pKa = 10.7LKK74 pKa = 11.09NKK76 pKa = 9.19VKK78 pKa = 10.72KK79 pKa = 10.16LLKK82 pKa = 10.81DD83 pKa = 3.32NGFLLEE89 pKa = 4.7DD90 pKa = 3.96SNDD93 pKa = 3.56QFEE96 pKa = 4.66VDD98 pKa = 3.29VKK100 pKa = 10.51IYY102 pKa = 10.28HH103 pKa = 6.3KK104 pKa = 11.04AMRR107 pKa = 11.84FNYY110 pKa = 10.06SEE112 pKa = 4.32EE113 pKa = 4.02LDD115 pKa = 3.55

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2E8Y8|I2E8Y8_9CAUD Gp43 OS=Clostridium phage PhiS63 OX=1187894 PE=4 SV=1
MM1 pKa = 7.35YY2 pKa = 9.87YY3 pKa = 10.64YY4 pKa = 10.17RR5 pKa = 11.84DD6 pKa = 3.2HH7 pKa = 7.23RR8 pKa = 11.84FNVARR13 pKa = 11.84LYY15 pKa = 9.89IQKK18 pKa = 10.12SGNIGILLNLKK29 pKa = 8.86VCRR32 pKa = 11.84IPIYY36 pKa = 10.19ILRR39 pKa = 11.84IDD41 pKa = 3.82QKK43 pKa = 10.85IKK45 pKa = 10.45PLALKK50 pKa = 10.31SQQ52 pKa = 3.48

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

9995

52

1084

232.4

26.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.792 ± 0.34

1.281 ± 0.17

6.233 ± 0.328

8.434 ± 0.525

4.132 ± 0.269

6.003 ± 0.455

1.161 ± 0.134

8.814 ± 0.238

11.486 ± 0.492

8.604 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.193

7.284 ± 0.411

2.091 ± 0.24

2.601 ± 0.185

3.842 ± 0.352

5.393 ± 0.255

5.233 ± 0.266

5.093 ± 0.354

0.96 ± 0.114

4.282 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski