Amino acid dipepetide frequency for Enterobacteria phage Sf101

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.83AlaAla: 9.83 ± 1.273
0.661AlaCys: 0.661 ± 0.199
5.7AlaAsp: 5.7 ± 0.698
7.765AlaGlu: 7.765 ± 1.545
3.387AlaPhe: 3.387 ± 0.624
6.691AlaGly: 6.691 ± 1.134
1.735AlaHis: 1.735 ± 0.368
6.113AlaIle: 6.113 ± 0.577
6.691AlaLys: 6.691 ± 1.123
7.434AlaLeu: 7.434 ± 0.81
3.882AlaMet: 3.882 ± 0.546
4.461AlaAsn: 4.461 ± 0.715
1.735AlaPro: 1.735 ± 0.326
5.039AlaGln: 5.039 ± 0.945
5.369AlaArg: 5.369 ± 0.73
4.626AlaSer: 4.626 ± 0.512
6.03AlaThr: 6.03 ± 0.759
4.295AlaVal: 4.295 ± 0.627
1.322AlaTrp: 1.322 ± 0.372
2.478AlaTyr: 2.478 ± 0.473
0.0AlaXaa: 0.0 ± 0.0
Cys
1.404CysAla: 1.404 ± 0.338
0.248CysCys: 0.248 ± 0.149
0.991CysAsp: 0.991 ± 0.26
0.991CysGlu: 0.991 ± 0.296
0.33CysPhe: 0.33 ± 0.17
1.487CysGly: 1.487 ± 0.318
0.413CysHis: 0.413 ± 0.206
0.496CysIle: 0.496 ± 0.184
0.991CysLys: 0.991 ± 0.27
1.156CysLeu: 1.156 ± 0.338
0.413CysMet: 0.413 ± 0.164
0.578CysAsn: 0.578 ± 0.221
0.33CysPro: 0.33 ± 0.182
0.248CysGln: 0.248 ± 0.159
0.826CysArg: 0.826 ± 0.256
0.661CysSer: 0.661 ± 0.223
0.083CysThr: 0.083 ± 0.079
0.578CysVal: 0.578 ± 0.212
0.248CysTrp: 0.248 ± 0.124
0.413CysTyr: 0.413 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
6.526AspAla: 6.526 ± 0.714
0.743AspCys: 0.743 ± 0.24
3.635AspAsp: 3.635 ± 0.598
3.965AspGlu: 3.965 ± 0.631
1.817AspPhe: 1.817 ± 0.345
4.791AspGly: 4.791 ± 0.61
0.826AspHis: 0.826 ± 0.246
3.8AspIle: 3.8 ± 0.522
2.809AspLys: 2.809 ± 0.377
4.13AspLeu: 4.13 ± 0.627
1.9AspMet: 1.9 ± 0.387
2.396AspAsn: 2.396 ± 0.573
2.065AspPro: 2.065 ± 0.474
1.156AspGln: 1.156 ± 0.323
3.304AspArg: 3.304 ± 0.506
3.882AspSer: 3.882 ± 0.484
3.222AspThr: 3.222 ± 0.507
3.717AspVal: 3.717 ± 0.4
0.909AspTrp: 0.909 ± 0.201
2.478AspTyr: 2.478 ± 0.393
0.0AspXaa: 0.0 ± 0.0
Glu
7.021GluAla: 7.021 ± 1.382
0.909GluCys: 0.909 ± 0.296
2.974GluAsp: 2.974 ± 0.589
5.782GluGlu: 5.782 ± 1.164
2.478GluPhe: 2.478 ± 0.399
3.965GluGly: 3.965 ± 0.589
1.404GluHis: 1.404 ± 0.27
4.213GluIle: 4.213 ± 0.766
4.626GluLys: 4.626 ± 0.703
7.269GluLeu: 7.269 ± 0.705
1.652GluMet: 1.652 ± 0.369
2.065GluAsn: 2.065 ± 0.364
1.487GluPro: 1.487 ± 0.333
4.213GluGln: 4.213 ± 0.762
4.791GluArg: 4.791 ± 0.938
4.295GluSer: 4.295 ± 0.701
1.9GluThr: 1.9 ± 0.501
3.469GluVal: 3.469 ± 0.48
1.239GluTrp: 1.239 ± 0.311
2.313GluTyr: 2.313 ± 0.425
0.0GluXaa: 0.0 ± 0.0
Phe
2.891PheAla: 2.891 ± 0.513
0.248PheCys: 0.248 ± 0.156
3.304PheAsp: 3.304 ± 0.534
2.478PheGlu: 2.478 ± 0.456
0.909PhePhe: 0.909 ± 0.502
2.313PheGly: 2.313 ± 0.32
0.578PheHis: 0.578 ± 0.229
2.809PheIle: 2.809 ± 0.725
1.569PheLys: 1.569 ± 0.434
1.982PheLeu: 1.982 ± 0.614
0.826PheMet: 0.826 ± 0.262
1.487PheAsn: 1.487 ± 0.318
0.991PhePro: 0.991 ± 0.296
1.404PheGln: 1.404 ± 0.362
2.065PheArg: 2.065 ± 0.37
1.9PheSer: 1.9 ± 0.351
1.9PheThr: 1.9 ± 0.453
1.817PheVal: 1.817 ± 0.296
0.743PheTrp: 0.743 ± 0.27
1.239PheTyr: 1.239 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
7.021GlyAla: 7.021 ± 0.899
0.909GlyCys: 0.909 ± 0.248
4.543GlyAsp: 4.543 ± 0.477
4.13GlyGlu: 4.13 ± 0.671
2.396GlyPhe: 2.396 ± 0.417
4.295GlyGly: 4.295 ± 0.71
1.487GlyHis: 1.487 ± 0.339
4.213GlyIle: 4.213 ± 0.752
4.378GlyLys: 4.378 ± 0.595
4.543GlyLeu: 4.543 ± 0.548
1.982GlyMet: 1.982 ± 0.408
3.139GlyAsn: 3.139 ± 0.55
1.156GlyPro: 1.156 ± 0.358
3.717GlyGln: 3.717 ± 0.69
3.965GlyArg: 3.965 ± 0.479
4.295GlySer: 4.295 ± 0.872
4.378GlyThr: 4.378 ± 0.733
5.121GlyVal: 5.121 ± 0.641
1.322GlyTrp: 1.322 ± 0.359
2.478GlyTyr: 2.478 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.239HisAla: 1.239 ± 0.238
0.33HisCys: 0.33 ± 0.175
1.156HisAsp: 1.156 ± 0.278
1.404HisGlu: 1.404 ± 0.359
0.578HisPhe: 0.578 ± 0.21
1.9HisGly: 1.9 ± 0.379
0.248HisHis: 0.248 ± 0.134
0.826HisIle: 0.826 ± 0.202
1.156HisLys: 1.156 ± 0.339
1.074HisLeu: 1.074 ± 0.251
0.33HisMet: 0.33 ± 0.144
0.248HisAsn: 0.248 ± 0.114
1.074HisPro: 1.074 ± 0.267
0.826HisGln: 0.826 ± 0.243
1.156HisArg: 1.156 ± 0.266
0.578HisSer: 0.578 ± 0.203
0.248HisThr: 0.248 ± 0.147
0.826HisVal: 0.826 ± 0.297
0.33HisTrp: 0.33 ± 0.158
0.661HisTyr: 0.661 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
5.452IleAla: 5.452 ± 0.567
0.909IleCys: 0.909 ± 0.308
3.635IleAsp: 3.635 ± 0.528
5.121IleGlu: 5.121 ± 0.638
1.404IlePhe: 1.404 ± 0.408
4.213IleGly: 4.213 ± 0.587
0.991IleHis: 0.991 ± 0.356
3.717IleIle: 3.717 ± 0.84
3.056IleLys: 3.056 ± 0.454
3.965IleLeu: 3.965 ± 0.595
1.322IleMet: 1.322 ± 0.317
3.635IleAsn: 3.635 ± 0.68
3.056IlePro: 3.056 ± 0.576
1.982IleGln: 1.982 ± 0.351
2.561IleArg: 2.561 ± 0.415
4.295IleSer: 4.295 ± 0.588
4.874IleThr: 4.874 ± 0.795
2.478IleVal: 2.478 ± 0.439
1.569IleTrp: 1.569 ± 0.447
1.239IleTyr: 1.239 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
8.013LysAla: 8.013 ± 1.077
0.413LysCys: 0.413 ± 0.182
3.139LysAsp: 3.139 ± 0.456
4.543LysGlu: 4.543 ± 0.78
1.735LysPhe: 1.735 ± 0.371
3.387LysGly: 3.387 ± 0.497
0.826LysHis: 0.826 ± 0.231
2.809LysIle: 2.809 ± 0.464
3.635LysLys: 3.635 ± 0.558
4.874LysLeu: 4.874 ± 0.622
2.313LysMet: 2.313 ± 0.371
2.891LysAsn: 2.891 ± 0.511
3.387LysPro: 3.387 ± 0.728
3.552LysGln: 3.552 ± 0.477
4.626LysArg: 4.626 ± 0.63
3.469LysSer: 3.469 ± 0.498
3.056LysThr: 3.056 ± 0.582
2.974LysVal: 2.974 ± 0.541
0.661LysTrp: 0.661 ± 0.227
2.065LysTyr: 2.065 ± 0.511
0.0LysXaa: 0.0 ± 0.0
Leu
8.591LeuAla: 8.591 ± 0.866
0.909LeuCys: 0.909 ± 0.274
3.717LeuAsp: 3.717 ± 0.647
5.039LeuGlu: 5.039 ± 0.745
2.891LeuPhe: 2.891 ± 0.806
4.295LeuGly: 4.295 ± 0.609
0.909LeuHis: 0.909 ± 0.246
4.461LeuIle: 4.461 ± 0.732
4.378LeuLys: 4.378 ± 0.653
6.774LeuLeu: 6.774 ± 0.876
2.891LeuMet: 2.891 ± 0.383
4.874LeuAsn: 4.874 ± 0.682
2.974LeuPro: 2.974 ± 0.436
3.717LeuGln: 3.717 ± 0.639
4.295LeuArg: 4.295 ± 0.68
4.626LeuSer: 4.626 ± 0.639
4.543LeuThr: 4.543 ± 0.527
4.378LeuVal: 4.378 ± 0.496
1.074LeuTrp: 1.074 ± 0.284
2.23LeuTyr: 2.23 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
3.222MetAla: 3.222 ± 0.467
0.496MetCys: 0.496 ± 0.187
1.569MetAsp: 1.569 ± 0.57
1.156MetGlu: 1.156 ± 0.305
0.826MetPhe: 0.826 ± 0.238
1.074MetGly: 1.074 ± 0.317
0.496MetHis: 0.496 ± 0.165
2.23MetIle: 2.23 ± 0.454
1.652MetLys: 1.652 ± 0.292
2.891MetLeu: 2.891 ± 0.42
0.578MetMet: 0.578 ± 0.235
1.156MetAsn: 1.156 ± 0.274
2.148MetPro: 2.148 ± 0.345
1.322MetGln: 1.322 ± 0.298
2.148MetArg: 2.148 ± 0.368
2.726MetSer: 2.726 ± 0.534
2.396MetThr: 2.396 ± 0.415
1.487MetVal: 1.487 ± 0.372
0.248MetTrp: 0.248 ± 0.129
0.826MetTyr: 0.826 ± 0.273
0.0MetXaa: 0.0 ± 0.0
Asn
4.708AsnAla: 4.708 ± 0.496
0.578AsnCys: 0.578 ± 0.176
2.478AsnAsp: 2.478 ± 0.583
2.065AsnGlu: 2.065 ± 0.414
0.33AsnPhe: 0.33 ± 0.149
5.369AsnGly: 5.369 ± 0.863
0.991AsnHis: 0.991 ± 0.361
2.478AsnIle: 2.478 ± 0.501
3.139AsnLys: 3.139 ± 0.483
3.387AsnLeu: 3.387 ± 0.455
1.074AsnMet: 1.074 ± 0.313
2.643AsnAsn: 2.643 ± 0.541
3.222AsnPro: 3.222 ± 0.692
3.056AsnGln: 3.056 ± 0.476
2.23AsnArg: 2.23 ± 0.445
2.891AsnSer: 2.891 ± 0.54
1.239AsnThr: 1.239 ± 0.332
2.561AsnVal: 2.561 ± 0.438
0.33AsnTrp: 0.33 ± 0.154
1.652AsnTyr: 1.652 ± 0.382
0.0AsnXaa: 0.0 ± 0.0
Pro
2.478ProAla: 2.478 ± 0.522
0.496ProCys: 0.496 ± 0.184
2.478ProAsp: 2.478 ± 0.512
4.048ProGlu: 4.048 ± 0.488
2.065ProPhe: 2.065 ± 0.462
2.313ProGly: 2.313 ± 0.456
0.413ProHis: 0.413 ± 0.168
1.652ProIle: 1.652 ± 0.452
2.643ProLys: 2.643 ± 0.448
2.396ProLeu: 2.396 ± 0.522
0.909ProMet: 0.909 ± 0.255
1.239ProAsn: 1.239 ± 0.277
1.404ProPro: 1.404 ± 0.331
1.735ProGln: 1.735 ± 0.457
1.9ProArg: 1.9 ± 0.325
2.891ProSer: 2.891 ± 0.412
1.735ProThr: 1.735 ± 0.383
2.974ProVal: 2.974 ± 0.507
0.578ProTrp: 0.578 ± 0.199
1.487ProTyr: 1.487 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
5.121GlnAla: 5.121 ± 1.102
0.496GlnCys: 0.496 ± 0.205
1.735GlnAsp: 1.735 ± 0.379
2.148GlnGlu: 2.148 ± 0.537
1.817GlnPhe: 1.817 ± 0.308
2.809GlnGly: 2.809 ± 0.593
0.413GlnHis: 0.413 ± 0.161
2.561GlnIle: 2.561 ± 0.39
3.717GlnLys: 3.717 ± 0.631
3.222GlnLeu: 3.222 ± 0.451
2.065GlnMet: 2.065 ± 0.361
2.396GlnAsn: 2.396 ± 0.521
1.652GlnPro: 1.652 ± 0.332
4.626GlnGln: 4.626 ± 0.941
3.635GlnArg: 3.635 ± 0.555
3.635GlnSer: 3.635 ± 0.466
2.148GlnThr: 2.148 ± 0.404
2.891GlnVal: 2.891 ± 0.431
1.156GlnTrp: 1.156 ± 0.298
1.9GlnTyr: 1.9 ± 0.468
0.0GlnXaa: 0.0 ± 0.0
Arg
3.882ArgAla: 3.882 ± 0.628
0.578ArgCys: 0.578 ± 0.219
2.974ArgAsp: 2.974 ± 0.529
4.295ArgGlu: 4.295 ± 0.954
1.9ArgPhe: 1.9 ± 0.384
3.552ArgGly: 3.552 ± 0.628
1.156ArgHis: 1.156 ± 0.359
3.965ArgIle: 3.965 ± 0.607
4.213ArgLys: 4.213 ± 0.633
5.452ArgLeu: 5.452 ± 0.611
2.396ArgMet: 2.396 ± 0.47
2.891ArgAsn: 2.891 ± 0.435
2.396ArgPro: 2.396 ± 0.453
3.222ArgGln: 3.222 ± 0.661
3.965ArgArg: 3.965 ± 0.736
3.139ArgSer: 3.139 ± 0.57
2.148ArgThr: 2.148 ± 0.393
3.304ArgVal: 3.304 ± 0.614
0.909ArgTrp: 0.909 ± 0.256
2.313ArgTyr: 2.313 ± 0.347
0.0ArgXaa: 0.0 ± 0.0
Ser
5.039SerAla: 5.039 ± 0.712
0.909SerCys: 0.909 ± 0.281
3.965SerAsp: 3.965 ± 0.465
3.717SerGlu: 3.717 ± 0.582
2.313SerPhe: 2.313 ± 0.438
5.452SerGly: 5.452 ± 0.576
0.991SerHis: 0.991 ± 0.271
3.8SerIle: 3.8 ± 0.503
3.304SerLys: 3.304 ± 0.691
5.534SerLeu: 5.534 ± 0.683
2.313SerMet: 2.313 ± 0.467
2.974SerAsn: 2.974 ± 0.409
2.891SerPro: 2.891 ± 0.379
3.056SerGln: 3.056 ± 0.564
3.387SerArg: 3.387 ± 0.479
4.708SerSer: 4.708 ± 0.788
2.809SerThr: 2.809 ± 0.462
3.882SerVal: 3.882 ± 0.545
0.991SerTrp: 0.991 ± 0.283
1.9SerTyr: 1.9 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
4.791ThrAla: 4.791 ± 0.613
0.991ThrCys: 0.991 ± 0.274
4.048ThrAsp: 4.048 ± 0.497
3.056ThrGlu: 3.056 ± 0.539
1.982ThrPhe: 1.982 ± 0.556
3.882ThrGly: 3.882 ± 0.545
0.578ThrHis: 0.578 ± 0.172
3.304ThrIle: 3.304 ± 0.567
3.965ThrLys: 3.965 ± 0.769
2.974ThrLeu: 2.974 ± 0.583
1.239ThrMet: 1.239 ± 0.347
1.569ThrAsn: 1.569 ± 0.241
2.891ThrPro: 2.891 ± 0.623
2.478ThrGln: 2.478 ± 0.51
2.396ThrArg: 2.396 ± 0.362
3.139ThrSer: 3.139 ± 0.493
3.8ThrThr: 3.8 ± 0.753
4.295ThrVal: 4.295 ± 0.63
0.743ThrTrp: 0.743 ± 0.265
1.569ThrTyr: 1.569 ± 0.396
0.0ThrXaa: 0.0 ± 0.0
Val
4.13ValAla: 4.13 ± 0.716
0.909ValCys: 0.909 ± 0.299
2.974ValAsp: 2.974 ± 0.624
4.295ValGlu: 4.295 ± 0.578
2.065ValPhe: 2.065 ± 0.357
4.295ValGly: 4.295 ± 0.579
0.826ValHis: 0.826 ± 0.26
3.387ValIle: 3.387 ± 0.383
3.8ValLys: 3.8 ± 0.626
4.048ValLeu: 4.048 ± 0.626
1.487ValMet: 1.487 ± 0.314
3.552ValAsn: 3.552 ± 0.477
1.569ValPro: 1.569 ± 0.401
1.817ValGln: 1.817 ± 0.377
2.313ValArg: 2.313 ± 0.354
5.204ValSer: 5.204 ± 0.815
4.295ValThr: 4.295 ± 0.578
3.635ValVal: 3.635 ± 0.661
0.496ValTrp: 0.496 ± 0.19
2.313ValTyr: 2.313 ± 0.713
0.0ValXaa: 0.0 ± 0.0
Trp
1.569TrpAla: 1.569 ± 0.352
0.578TrpCys: 0.578 ± 0.231
0.413TrpAsp: 0.413 ± 0.208
0.413TrpGlu: 0.413 ± 0.212
0.826TrpPhe: 0.826 ± 0.27
0.743TrpGly: 0.743 ± 0.258
0.33TrpHis: 0.33 ± 0.169
0.743TrpIle: 0.743 ± 0.256
0.826TrpLys: 0.826 ± 0.302
1.9TrpLeu: 1.9 ± 0.382
0.33TrpMet: 0.33 ± 0.142
0.909TrpAsn: 0.909 ± 0.311
0.413TrpPro: 0.413 ± 0.19
0.661TrpGln: 0.661 ± 0.249
1.322TrpArg: 1.322 ± 0.294
1.074TrpSer: 1.074 ± 0.281
0.826TrpThr: 0.826 ± 0.282
0.826TrpVal: 0.826 ± 0.285
0.248TrpTrp: 0.248 ± 0.111
0.578TrpTyr: 0.578 ± 0.25
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.313TyrAla: 2.313 ± 0.533
0.578TyrCys: 0.578 ± 0.209
2.643TyrAsp: 2.643 ± 0.653
1.652TyrGlu: 1.652 ± 0.337
1.404TyrPhe: 1.404 ± 0.396
2.643TyrGly: 2.643 ± 0.471
0.578TyrHis: 0.578 ± 0.174
1.9TyrIle: 1.9 ± 0.384
1.817TyrLys: 1.817 ± 0.349
2.561TyrLeu: 2.561 ± 0.444
0.661TyrMet: 0.661 ± 0.199
1.404TyrAsn: 1.404 ± 0.343
0.991TyrPro: 0.991 ± 0.274
2.313TyrGln: 2.313 ± 0.517
2.396TyrArg: 2.396 ± 0.485
1.9TyrSer: 1.9 ± 0.401
2.065TyrThr: 2.065 ± 0.396
1.9TyrVal: 1.9 ± 0.372
0.413TyrTrp: 0.413 ± 0.212
0.826TyrTyr: 0.826 ± 0.271
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski