Amino acid dipepetide frequency for Arthrobacter sp. Edens01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.031AlaAla: 22.031 ± 0.263
0.753AlaCys: 0.753 ± 0.03
7.165AlaAsp: 7.165 ± 0.109
8.944AlaGlu: 8.944 ± 0.114
3.738AlaPhe: 3.738 ± 0.067
13.759AlaGly: 13.759 ± 0.149
1.973AlaHis: 1.973 ± 0.046
4.382AlaIle: 4.382 ± 0.073
3.162AlaLys: 3.162 ± 0.064
13.432AlaLeu: 13.432 ± 0.145
2.841AlaMet: 2.841 ± 0.056
2.595AlaAsn: 2.595 ± 0.054
6.196AlaPro: 6.196 ± 0.107
3.932AlaGln: 3.932 ± 0.07
8.115AlaArg: 8.115 ± 0.107
6.966AlaSer: 6.966 ± 0.092
5.708AlaThr: 5.708 ± 0.075
11.745AlaVal: 11.745 ± 0.118
1.744AlaTrp: 1.744 ± 0.045
2.417AlaTyr: 2.417 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.72CysAla: 0.72 ± 0.026
0.062CysCys: 0.062 ± 0.009
0.304CysAsp: 0.304 ± 0.016
0.296CysGlu: 0.296 ± 0.018
0.19CysPhe: 0.19 ± 0.012
0.699CysGly: 0.699 ± 0.027
0.119CysHis: 0.119 ± 0.012
0.247CysIle: 0.247 ± 0.016
0.075CysLys: 0.075 ± 0.009
0.577CysLeu: 0.577 ± 0.025
0.088CysMet: 0.088 ± 0.008
0.118CysAsn: 0.118 ± 0.01
0.335CysPro: 0.335 ± 0.018
0.127CysGln: 0.127 ± 0.011
0.377CysArg: 0.377 ± 0.022
0.421CysSer: 0.421 ± 0.02
0.402CysThr: 0.402 ± 0.023
0.427CysVal: 0.427 ± 0.021
0.093CysTrp: 0.093 ± 0.009
0.118CysTyr: 0.118 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.237AspAla: 7.237 ± 0.1
0.293AspCys: 0.293 ± 0.018
2.854AspAsp: 2.854 ± 0.064
3.5AspGlu: 3.5 ± 0.07
1.842AspPhe: 1.842 ± 0.044
5.597AspGly: 5.597 ± 0.073
1.021AspHis: 1.021 ± 0.033
2.328AspIle: 2.328 ± 0.049
1.196AspLys: 1.196 ± 0.037
5.944AspLeu: 5.944 ± 0.08
0.999AspMet: 0.999 ± 0.034
1.041AspAsn: 1.041 ± 0.034
4.108AspPro: 4.108 ± 0.077
1.567AspGln: 1.567 ± 0.042
3.574AspArg: 3.574 ± 0.062
3.041AspSer: 3.041 ± 0.053
2.878AspThr: 2.878 ± 0.057
4.686AspVal: 4.686 ± 0.069
0.889AspTrp: 0.889 ± 0.034
1.413AspTyr: 1.413 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
7.829GluAla: 7.829 ± 0.112
0.253GluCys: 0.253 ± 0.015
3.503GluAsp: 3.503 ± 0.064
3.949GluGlu: 3.949 ± 0.086
1.743GluPhe: 1.743 ± 0.041
4.286GluGly: 4.286 ± 0.059
1.409GluHis: 1.409 ± 0.036
2.691GluIle: 2.691 ± 0.057
1.794GluLys: 1.794 ± 0.045
7.071GluLeu: 7.071 ± 0.1
1.098GluMet: 1.098 ± 0.034
1.755GluAsn: 1.755 ± 0.047
3.229GluPro: 3.229 ± 0.064
2.342GluGln: 2.342 ± 0.053
4.335GluArg: 4.335 ± 0.072
3.194GluSer: 3.194 ± 0.049
3.443GluThr: 3.443 ± 0.062
4.493GluVal: 4.493 ± 0.076
0.746GluTrp: 0.746 ± 0.028
1.259GluTyr: 1.259 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
3.98PheAla: 3.98 ± 0.061
0.251PheCys: 0.251 ± 0.017
2.038PheAsp: 2.038 ± 0.048
1.788PheGlu: 1.788 ± 0.042
1.161PhePhe: 1.161 ± 0.039
3.389PheGly: 3.389 ± 0.074
0.593PheHis: 0.593 ± 0.024
1.341PheIle: 1.341 ± 0.04
0.699PheLys: 0.699 ± 0.029
3.085PheLeu: 3.085 ± 0.069
0.571PheMet: 0.571 ± 0.024
0.89PheAsn: 0.89 ± 0.03
1.497PhePro: 1.497 ± 0.037
0.853PheGln: 0.853 ± 0.032
1.88PheArg: 1.88 ± 0.042
2.129PheSer: 2.129 ± 0.043
2.141PheThr: 2.141 ± 0.046
2.433PheVal: 2.433 ± 0.053
0.499PheTrp: 0.499 ± 0.024
0.733PheTyr: 0.733 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
10.263GlyAla: 10.263 ± 0.135
0.613GlyCys: 0.613 ± 0.024
4.315GlyAsp: 4.315 ± 0.066
4.86GlyGlu: 4.86 ± 0.076
3.337GlyPhe: 3.337 ± 0.066
7.663GlyGly: 7.663 ± 0.128
1.851GlyHis: 1.851 ± 0.044
4.766GlyIle: 4.766 ± 0.072
2.812GlyLys: 2.812 ± 0.059
9.27GlyLeu: 9.27 ± 0.118
2.229GlyMet: 2.229 ± 0.05
2.348GlyAsn: 2.348 ± 0.053
4.265GlyPro: 4.265 ± 0.079
2.843GlyGln: 2.843 ± 0.056
6.029GlyArg: 6.029 ± 0.075
5.961GlySer: 5.961 ± 0.087
6.505GlyThr: 6.505 ± 0.087
7.234GlyVal: 7.234 ± 0.102
1.6GlyTrp: 1.6 ± 0.038
2.433GlyTyr: 2.433 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.005HisAla: 2.005 ± 0.039
0.14HisCys: 0.14 ± 0.013
0.99HisAsp: 0.99 ± 0.03
1.022HisGlu: 1.022 ± 0.03
0.62HisPhe: 0.62 ± 0.025
1.866HisGly: 1.866 ± 0.044
0.51HisHis: 0.51 ± 0.026
0.714HisIle: 0.714 ± 0.025
0.381HisLys: 0.381 ± 0.022
1.986HisLeu: 1.986 ± 0.047
0.347HisMet: 0.347 ± 0.017
0.465HisAsn: 0.465 ± 0.02
1.383HisPro: 1.383 ± 0.04
0.573HisGln: 0.573 ± 0.022
1.451HisArg: 1.451 ± 0.035
1.112HisSer: 1.112 ± 0.034
1.035HisThr: 1.035 ± 0.035
1.429HisVal: 1.429 ± 0.036
0.276HisTrp: 0.276 ± 0.016
0.44HisTyr: 0.44 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.41IleAla: 5.41 ± 0.082
0.326IleCys: 0.326 ± 0.019
2.595IleAsp: 2.595 ± 0.051
2.352IleGlu: 2.352 ± 0.052
1.331IlePhe: 1.331 ± 0.041
4.09IleGly: 4.09 ± 0.071
0.793IleHis: 0.793 ± 0.032
1.849IleIle: 1.849 ± 0.048
0.97IleLys: 0.97 ± 0.033
4.088IleLeu: 4.088 ± 0.082
0.753IleMet: 0.753 ± 0.03
1.154IleAsn: 1.154 ± 0.034
2.458IlePro: 2.458 ± 0.054
1.144IleGln: 1.144 ± 0.038
2.717IleArg: 2.717 ± 0.049
2.722IleSer: 2.722 ± 0.05
2.735IleThr: 2.735 ± 0.055
3.378IleVal: 3.378 ± 0.067
0.477IleTrp: 0.477 ± 0.021
0.857IleTyr: 0.857 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.119LysAla: 3.119 ± 0.06
0.076LysCys: 0.076 ± 0.008
1.53LysAsp: 1.53 ± 0.04
1.412LysGlu: 1.412 ± 0.039
0.604LysPhe: 0.604 ± 0.024
1.805LysGly: 1.805 ± 0.048
0.512LysHis: 0.512 ± 0.022
1.139LysIle: 1.139 ± 0.032
0.872LysLys: 0.872 ± 0.035
2.449LysLeu: 2.449 ± 0.052
0.578LysMet: 0.578 ± 0.024
0.661LysAsn: 0.661 ± 0.028
1.547LysPro: 1.547 ± 0.042
0.778LysGln: 0.778 ± 0.03
1.559LysArg: 1.559 ± 0.039
1.395LysSer: 1.395 ± 0.038
1.58LysThr: 1.58 ± 0.041
2.14LysVal: 2.14 ± 0.046
0.288LysTrp: 0.288 ± 0.019
0.61LysTyr: 0.61 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
14.89LeuAla: 14.89 ± 0.164
0.607LeuCys: 0.607 ± 0.022
6.337LeuAsp: 6.337 ± 0.084
6.024LeuGlu: 6.024 ± 0.089
3.017LeuPhe: 3.017 ± 0.063
9.318LeuGly: 9.318 ± 0.133
1.933LeuHis: 1.933 ± 0.048
4.363LeuIle: 4.363 ± 0.081
2.404LeuLys: 2.404 ± 0.055
11.123LeuLeu: 11.123 ± 0.154
2.004LeuMet: 2.004 ± 0.045
2.47LeuAsn: 2.47 ± 0.054
6.036LeuPro: 6.036 ± 0.079
3.165LeuGln: 3.165 ± 0.052
7.145LeuArg: 7.145 ± 0.107
6.38LeuSer: 6.38 ± 0.087
6.168LeuThr: 6.168 ± 0.087
8.343LeuVal: 8.343 ± 0.098
1.3LeuTrp: 1.3 ± 0.039
1.8LeuTyr: 1.8 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.645MetAla: 2.645 ± 0.048
0.111MetCys: 0.111 ± 0.011
1.187MetAsp: 1.187 ± 0.034
1.036MetGlu: 1.036 ± 0.031
0.599MetPhe: 0.599 ± 0.026
1.64MetGly: 1.64 ± 0.047
0.355MetHis: 0.355 ± 0.02
0.879MetIle: 0.879 ± 0.03
0.574MetLys: 0.574 ± 0.023
2.041MetLeu: 2.041 ± 0.047
0.38MetMet: 0.38 ± 0.017
0.598MetAsn: 0.598 ± 0.025
1.187MetPro: 1.187 ± 0.036
0.62MetGln: 0.62 ± 0.026
1.297MetArg: 1.297 ± 0.038
1.536MetSer: 1.536 ± 0.039
1.45MetThr: 1.45 ± 0.035
1.651MetVal: 1.651 ± 0.038
0.212MetTrp: 0.212 ± 0.014
0.354MetTyr: 0.354 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.876AsnAla: 2.876 ± 0.057
0.14AsnCys: 0.14 ± 0.013
1.178AsnAsp: 1.178 ± 0.036
1.252AsnGlu: 1.252 ± 0.039
0.818AsnPhe: 0.818 ± 0.031
2.266AsnGly: 2.266 ± 0.057
0.46AsnHis: 0.46 ± 0.019
1.055AsnIle: 1.055 ± 0.029
0.57AsnLys: 0.57 ± 0.026
2.485AsnLeu: 2.485 ± 0.055
0.461AsnMet: 0.461 ± 0.018
0.68AsnAsn: 0.68 ± 0.032
1.871AsnPro: 1.871 ± 0.043
0.734AsnGln: 0.734 ± 0.021
1.594AsnArg: 1.594 ± 0.045
1.375AsnSer: 1.375 ± 0.039
1.421AsnThr: 1.421 ± 0.038
1.786AsnVal: 1.786 ± 0.042
0.372AsnTrp: 0.372 ± 0.02
0.593AsnTyr: 0.593 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
8.11ProAla: 8.11 ± 0.118
0.2ProCys: 0.2 ± 0.014
3.815ProAsp: 3.815 ± 0.069
4.622ProGlu: 4.622 ± 0.076
1.685ProPhe: 1.685 ± 0.045
5.808ProGly: 5.808 ± 0.088
0.966ProHis: 0.966 ± 0.034
1.564ProIle: 1.564 ± 0.046
1.318ProLys: 1.318 ± 0.036
5.13ProLeu: 5.13 ± 0.063
0.974ProMet: 0.974 ± 0.032
1.118ProAsn: 1.118 ± 0.028
2.281ProPro: 2.281 ± 0.057
1.829ProGln: 1.829 ± 0.044
3.103ProArg: 3.103 ± 0.066
3.21ProSer: 3.21 ± 0.053
2.4ProThr: 2.4 ± 0.05
5.179ProVal: 5.179 ± 0.074
0.77ProTrp: 0.77 ± 0.024
1.095ProTyr: 1.095 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
4.16GlnAla: 4.16 ± 0.074
0.139GlnCys: 0.139 ± 0.011
1.837GlnAsp: 1.837 ± 0.043
1.92GlnGlu: 1.92 ± 0.051
0.849GlnPhe: 0.849 ± 0.033
2.335GlnGly: 2.335 ± 0.051
0.615GlnHis: 0.615 ± 0.026
1.41GlnIle: 1.41 ± 0.04
0.766GlnLys: 0.766 ± 0.029
3.502GlnLeu: 3.502 ± 0.059
0.654GlnMet: 0.654 ± 0.028
0.729GlnAsn: 0.729 ± 0.03
1.749GlnPro: 1.749 ± 0.047
1.307GlnGln: 1.307 ± 0.038
2.38GlnArg: 2.38 ± 0.046
1.623GlnSer: 1.623 ± 0.037
1.545GlnThr: 1.545 ± 0.038
2.497GlnVal: 2.497 ± 0.049
0.475GlnTrp: 0.475 ± 0.021
0.644GlnTyr: 0.644 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
7.249ArgAla: 7.249 ± 0.107
0.369ArgCys: 0.369 ± 0.016
3.449ArgAsp: 3.449 ± 0.061
4.169ArgGlu: 4.169 ± 0.066
2.322ArgPhe: 2.322 ± 0.051
4.973ArgGly: 4.973 ± 0.068
1.371ArgHis: 1.371 ± 0.034
3.458ArgIle: 3.458 ± 0.058
1.757ArgLys: 1.757 ± 0.046
6.984ArgLeu: 6.984 ± 0.102
1.598ArgMet: 1.598 ± 0.042
1.728ArgAsn: 1.728 ± 0.045
3.391ArgPro: 3.391 ± 0.06
2.334ArgGln: 2.334 ± 0.052
5.996ArgArg: 5.996 ± 0.104
4.279ArgSer: 4.279 ± 0.073
4.152ArgThr: 4.152 ± 0.073
4.779ArgVal: 4.779 ± 0.064
1.112ArgTrp: 1.112 ± 0.032
1.565ArgTyr: 1.565 ± 0.045
0.001ArgXaa: 0.001 ± 0.001
Ser
7.687SerAla: 7.687 ± 0.096
0.329SerCys: 0.329 ± 0.022
2.886SerAsp: 2.886 ± 0.061
3.29SerGlu: 3.29 ± 0.048
1.981SerPhe: 1.981 ± 0.044
6.438SerGly: 6.438 ± 0.094
1.014SerHis: 1.014 ± 0.033
2.382SerIle: 2.382 ± 0.049
1.389SerLys: 1.389 ± 0.038
6.041SerLeu: 6.041 ± 0.085
1.464SerMet: 1.464 ± 0.036
1.351SerAsn: 1.351 ± 0.041
3.305SerPro: 3.305 ± 0.059
1.612SerGln: 1.612 ± 0.044
4.069SerArg: 4.069 ± 0.07
3.753SerSer: 3.753 ± 0.068
3.436SerThr: 3.436 ± 0.054
4.879SerVal: 4.879 ± 0.079
0.942SerTrp: 0.942 ± 0.033
1.379SerTyr: 1.379 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
7.78ThrAla: 7.78 ± 0.086
0.336ThrCys: 0.336 ± 0.018
3.188ThrAsp: 3.188 ± 0.06
3.284ThrGlu: 3.284 ± 0.054
1.804ThrPhe: 1.804 ± 0.039
6.15ThrGly: 6.15 ± 0.087
1.0ThrHis: 1.0 ± 0.03
2.25ThrIle: 2.25 ± 0.049
1.223ThrLys: 1.223 ± 0.035
5.587ThrLeu: 5.587 ± 0.077
1.003ThrMet: 1.003 ± 0.036
1.254ThrAsn: 1.254 ± 0.035
3.468ThrPro: 3.468 ± 0.059
1.498ThrGln: 1.498 ± 0.04
3.291ThrArg: 3.291 ± 0.055
3.294ThrSer: 3.294 ± 0.056
3.107ThrThr: 3.107 ± 0.053
5.477ThrVal: 5.477 ± 0.075
0.778ThrTrp: 0.778 ± 0.029
1.247ThrTyr: 1.247 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
9.574ValAla: 9.574 ± 0.114
0.544ValCys: 0.544 ± 0.026
4.783ValAsp: 4.783 ± 0.069
4.784ValGlu: 4.784 ± 0.067
2.959ValPhe: 2.959 ± 0.059
6.051ValGly: 6.051 ± 0.093
1.601ValHis: 1.601 ± 0.043
3.933ValIle: 3.933 ± 0.068
1.793ValLys: 1.793 ± 0.049
9.915ValLeu: 9.915 ± 0.12
1.619ValMet: 1.619 ± 0.042
2.005ValAsn: 2.005 ± 0.051
5.001ValPro: 5.001 ± 0.076
2.597ValGln: 2.597 ± 0.055
5.503ValArg: 5.503 ± 0.08
5.169ValSer: 5.169 ± 0.069
4.878ValThr: 4.878 ± 0.069
7.411ValVal: 7.411 ± 0.106
0.986ValTrp: 0.986 ± 0.032
1.56ValTyr: 1.56 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.464TrpAla: 1.464 ± 0.039
0.101TrpCys: 0.101 ± 0.01
0.813TrpAsp: 0.813 ± 0.03
0.71TrpGlu: 0.71 ± 0.027
0.56TrpPhe: 0.56 ± 0.027
0.944TrpGly: 0.944 ± 0.032
0.327TrpHis: 0.327 ± 0.017
0.716TrpIle: 0.716 ± 0.024
0.412TrpLys: 0.412 ± 0.021
1.758TrpLeu: 1.758 ± 0.052
0.354TrpMet: 0.354 ± 0.016
0.518TrpAsn: 0.518 ± 0.023
0.671TrpPro: 0.671 ± 0.03
0.62TrpGln: 0.62 ± 0.025
0.965TrpArg: 0.965 ± 0.034
0.775TrpSer: 0.775 ± 0.031
0.847TrpThr: 0.847 ± 0.027
1.058TrpVal: 1.058 ± 0.033
0.33TrpTrp: 0.33 ± 0.017
0.321TrpTyr: 0.321 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.569TyrAla: 2.569 ± 0.06
0.155TyrCys: 0.155 ± 0.011
1.242TyrAsp: 1.242 ± 0.039
1.207TyrGlu: 1.207 ± 0.039
0.787TyrPhe: 0.787 ± 0.031
2.118TyrGly: 2.118 ± 0.052
0.322TyrHis: 0.322 ± 0.018
0.732TyrIle: 0.732 ± 0.029
0.488TyrLys: 0.488 ± 0.023
2.358TyrLeu: 2.358 ± 0.055
0.338TyrMet: 0.338 ± 0.02
0.507TyrAsn: 0.507 ± 0.026
1.198TyrPro: 1.198 ± 0.034
0.661TyrGln: 0.661 ± 0.026
1.657TyrArg: 1.657 ± 0.043
1.294TyrSer: 1.294 ± 0.039
1.189TyrThr: 1.189 ± 0.035
1.64TyrVal: 1.64 ± 0.043
0.376TyrTrp: 0.376 ± 0.018
0.502TyrTyr: 0.502 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.001XaaGlu: 0.001 ± 0.001
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.002XaaXaa: 0.002 ± 0.002
Statistics based on 3212 proteins (1044346 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski