Thiothrix eikelboomii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Thiothrix

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3906 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4XKW8|A0A1T4XKW8_9GAMM Uncharacterized protein OS=Thiothrix eikelboomii OX=92487 GN=SAMN02745130_03104 PE=3 SV=1
MM1 pKa = 7.42SNVPANLKK9 pKa = 10.19YY10 pKa = 10.6ADD12 pKa = 3.61SHH14 pKa = 5.56EE15 pKa = 4.62WILDD19 pKa = 3.62HH20 pKa = 7.65GDD22 pKa = 3.31GTVTIGITDD31 pKa = 3.96HH32 pKa = 6.71AQALLGDD39 pKa = 4.35LVFVEE44 pKa = 4.81LPEE47 pKa = 5.3IGSILSAGDD56 pKa = 3.07EE57 pKa = 4.44CGVVEE62 pKa = 4.4SVKK65 pKa = 10.57AASDD69 pKa = 3.49IYY71 pKa = 11.25APIGGEE77 pKa = 3.91VFEE80 pKa = 5.1VNHH83 pKa = 6.31EE84 pKa = 4.26LDD86 pKa = 4.59DD87 pKa = 4.61SPEE90 pKa = 4.06LVNTDD95 pKa = 3.56PYY97 pKa = 9.23EE98 pKa = 4.33AGWIFKK104 pKa = 10.58LKK106 pKa = 10.76LEE108 pKa = 4.41NSSDD112 pKa = 3.56LAALLSADD120 pKa = 3.73AYY122 pKa = 10.28GEE124 pKa = 4.23LVASEE129 pKa = 4.21AA130 pKa = 4.02

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4VRE8|A0A1T4VRE8_9GAMM Transcriptional regulator TetR family OS=Thiothrix eikelboomii OX=92487 GN=SAMN02745130_00056 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.1FQLLIEE8 pKa = 4.34EE9 pKa = 4.24QQGRR13 pKa = 11.84ILDD16 pKa = 3.79VATSAEE22 pKa = 4.12QEE24 pKa = 4.05HH25 pKa = 7.02DD26 pKa = 3.24LCLARR31 pKa = 11.84RR32 pKa = 11.84HH33 pKa = 5.43AHH35 pKa = 6.25HH36 pKa = 7.03LPAGSVCAVDD46 pKa = 3.8LGYY49 pKa = 11.23VGFRR53 pKa = 11.84VEE55 pKa = 4.21GCSVVMPFKK64 pKa = 10.89KK65 pKa = 10.13PLGRR69 pKa = 11.84DD70 pKa = 3.49LEE72 pKa = 4.43PEE74 pKa = 3.83QIEE77 pKa = 4.34FNQRR81 pKa = 11.84FAKK84 pKa = 10.28VRR86 pKa = 11.84VKK88 pKa = 10.46VEE90 pKa = 3.39HH91 pKa = 7.3RR92 pKa = 11.84IRR94 pKa = 11.84SLKK97 pKa = 9.89IFRR100 pKa = 11.84ILKK103 pKa = 8.51GVYY106 pKa = 8.79RR107 pKa = 11.84GRR109 pKa = 11.84RR110 pKa = 11.84QRR112 pKa = 11.84FGLRR116 pKa = 11.84LRR118 pKa = 11.84LIAALVNRR126 pKa = 11.84MIGGG130 pKa = 3.59

Molecular weight: 14.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3906

0

3906

1248811

39

4364

319.7

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.113 ± 0.05

0.954 ± 0.012

4.891 ± 0.032

5.825 ± 0.033

3.781 ± 0.027

6.97 ± 0.046

2.3 ± 0.021

5.566 ± 0.032

4.423 ± 0.037

11.481 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.369 ± 0.024

3.609 ± 0.03

4.605 ± 0.028

5.373 ± 0.037

5.251 ± 0.035

5.908 ± 0.035

5.571 ± 0.037

6.556 ± 0.035

1.419 ± 0.017

3.034 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski