Bdellovibrio sp. NC01

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales; Bdellovibrionaceae; Bdellovibrio; unclassified Bdellovibrio

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514WT11|A0A514WT11_9PROT 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Bdellovibrio sp. NC01 OX=2220073 GN=menA PE=3 SV=1
MM1 pKa = 7.5LACPSCQKK9 pKa = 10.3PVEE12 pKa = 4.29ILDD15 pKa = 3.65KK16 pKa = 11.15HH17 pKa = 6.67LGTLFTCPHH26 pKa = 6.71CGAVYY31 pKa = 9.85FIDD34 pKa = 5.35FNGQPEE40 pKa = 4.44MANHH44 pKa = 5.76EE45 pKa = 4.5AEE47 pKa = 4.48PDD49 pKa = 3.44EE50 pKa = 4.72TPAQSFEE57 pKa = 4.63TPVSEE62 pKa = 4.78QPADD66 pKa = 3.78FSDD69 pKa = 4.17GQDD72 pKa = 3.31FANQGSQDD80 pKa = 3.66FGQSPVATDD89 pKa = 5.05FSAEE93 pKa = 4.19TNYY96 pKa = 9.6STDD99 pKa = 3.9QNFQQQGDD107 pKa = 4.0FQSQDD112 pKa = 2.76GFQQNGFEE120 pKa = 4.27QNNVEE125 pKa = 4.18QSADD129 pKa = 3.56QFGSPASVYY138 pKa = 11.0NNDD141 pKa = 2.94SHH143 pKa = 7.32GVGGQAVEE151 pKa = 4.06QNFGGEE157 pKa = 3.8QSYY160 pKa = 11.03QDD162 pKa = 3.7SSAEE166 pKa = 4.09YY167 pKa = 10.5ADD169 pKa = 4.72SADD172 pKa = 3.43QEE174 pKa = 4.59APVAEE179 pKa = 4.61APVEE183 pKa = 3.89EE184 pKa = 5.09APFDD188 pKa = 4.26FNATLDD194 pKa = 4.01QQPMQAPPPACVSDD208 pKa = 3.6SADD211 pKa = 3.49FSDD214 pKa = 3.93VTDD217 pKa = 4.5FANADD222 pKa = 3.66TTAGPLTYY230 pKa = 10.62VVIIDD235 pKa = 5.05GIEE238 pKa = 3.99SSQLLYY244 pKa = 10.34QLKK247 pKa = 9.98EE248 pKa = 3.75AMTDD252 pKa = 3.52SRR254 pKa = 11.84FGWDD258 pKa = 3.24VNEE261 pKa = 5.4LLTQVGGGRR270 pKa = 11.84LVIPGLNPAKK280 pKa = 10.44ASVLIGRR287 pKa = 11.84IKK289 pKa = 10.52YY290 pKa = 10.47LPFKK294 pKa = 10.54ISWRR298 pKa = 11.84QDD300 pKa = 3.29VLSGSS305 pKa = 3.67

Molecular weight: 32.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514WZ62|A0A514WZ62_9PROT 50S ribosomal protein L2 OS=Bdellovibrio sp. NC01 OX=2220073 GN=rplB PE=3 SV=1
MM1 pKa = 7.41NSSPADD7 pKa = 3.5KK8 pKa = 10.69NRR10 pKa = 11.84QLTAKK15 pKa = 9.86KK16 pKa = 10.04PSNTHH21 pKa = 3.79QNGRR25 pKa = 11.84FFYY28 pKa = 10.5CFRR31 pKa = 11.84ALFSGFFMPLRR42 pKa = 11.84YY43 pKa = 9.74GFQRR47 pKa = 11.84EE48 pKa = 4.3TTVFWAAFLEE58 pKa = 4.52LGFSHH63 pKa = 6.29IQFYY67 pKa = 11.01AKK69 pKa = 9.96QRR71 pKa = 11.84WGFGLDD77 pKa = 3.11LTPCVRR83 pKa = 11.84VKK85 pKa = 10.63LSKK88 pKa = 10.84SFIKK92 pKa = 10.22NWPGRR97 pKa = 11.84QISAAPVRR105 pKa = 11.84EE106 pKa = 4.02QGQEE110 pKa = 3.58PSIKK114 pKa = 9.84IRR116 pKa = 3.48

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3773

0

3773

1203359

36

2902

318.9

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.553 ± 0.048

0.931 ± 0.013

5.169 ± 0.026

6.143 ± 0.054

4.603 ± 0.031

7.012 ± 0.048

1.879 ± 0.018

5.948 ± 0.031

6.897 ± 0.05

9.521 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.023

4.397 ± 0.033

4.036 ± 0.027

3.955 ± 0.027

4.371 ± 0.03

7.028 ± 0.052

5.725 ± 0.061

6.916 ± 0.031

1.186 ± 0.014

3.116 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski