Haliscomenobacter hydrossis (strain ATCC 27775 / DSM 1100 / LMG 10767 / O)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Haliscomenobacteraceae; Haliscomenobacter; Haliscomenobacter hydrossis

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6704 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4KTT8|F4KTT8_HALH1 Glycosyl hydrolase BNR repeat-containing protein OS=Haliscomenobacter hydrossis (strain ATCC 27775 / DSM 1100 / LMG 10767 / O) OX=760192 GN=Halhy_0169 PE=4 SV=1
MM1 pKa = 7.29YY2 pKa = 7.8WTLDD6 pKa = 3.83LASHH10 pKa = 6.92LEE12 pKa = 4.1DD13 pKa = 3.83APYY16 pKa = 9.96PATRR20 pKa = 11.84DD21 pKa = 3.53EE22 pKa = 5.23LIDD25 pKa = 3.48YY26 pKa = 8.81AIRR29 pKa = 11.84SGSPKK34 pKa = 10.27EE35 pKa = 4.12VIEE38 pKa = 4.16NLQAMEE44 pKa = 4.97DD45 pKa = 3.86DD46 pKa = 4.37GEE48 pKa = 4.2EE49 pKa = 4.28EE50 pKa = 4.36YY51 pKa = 11.18EE52 pKa = 4.35SIEE55 pKa = 5.23DD56 pKa = 3.36IWPDD60 pKa = 3.63YY61 pKa = 10.11PKK63 pKa = 10.86KK64 pKa = 10.66DD65 pKa = 3.34DD66 pKa = 4.57FLFNDD71 pKa = 4.38DD72 pKa = 4.24EE73 pKa = 4.63YY74 pKa = 11.98

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4L036|F4L036_HALH1 Uncharacterized protein OS=Haliscomenobacter hydrossis (strain ATCC 27775 / DSM 1100 / LMG 10767 / O) OX=760192 GN=Halhy_4916 PE=4 SV=1
MM1 pKa = 6.97LQKK4 pKa = 10.54RR5 pKa = 11.84NPRR8 pKa = 11.84CHH10 pKa = 6.52IPQFLEE16 pKa = 4.09VKK18 pKa = 9.02TGCSTKK24 pKa = 10.39RR25 pKa = 11.84FNLLYY30 pKa = 10.63CMCLNSKK37 pKa = 10.17GLLQYY42 pKa = 8.77PFPWPQTQQWPQYY55 pKa = 9.15LAQAKK60 pKa = 10.1IPGRR64 pKa = 11.84PIGKK68 pKa = 7.99TDD70 pKa = 3.4TNVSFWIQMLTRR82 pKa = 11.84PLQVLRR88 pKa = 11.84QVFRR92 pKa = 11.84TKK94 pKa = 9.11VTHH97 pKa = 4.34QTKK100 pKa = 10.13HH101 pKa = 5.94KK102 pKa = 10.26NVVKK106 pKa = 8.85FLCWGRR112 pKa = 11.84PIADD116 pKa = 4.26FSYY119 pKa = 10.48RR120 pKa = 11.84ALCNGYY126 pKa = 9.1NALSQHH132 pKa = 4.93RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84YY136 pKa = 8.37ISRR139 pKa = 11.84VVSKK143 pKa = 11.14GGGFFMQCLII153 pKa = 4.44

Molecular weight: 18.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6704

0

6704

2562877

30

5481

382.3

42.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.836 ± 0.028

0.955 ± 0.017

5.286 ± 0.04

5.891 ± 0.034

4.894 ± 0.025

7.073 ± 0.034

1.927 ± 0.015

6.347 ± 0.024

5.889 ± 0.037

10.141 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.019

5.062 ± 0.028

4.339 ± 0.022

4.354 ± 0.022

4.621 ± 0.025

6.12 ± 0.03

5.595 ± 0.042

6.276 ± 0.027

1.437 ± 0.012

3.783 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski