Lactobacillus bifermentans DSM 20003

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Loigolactobacillus; Loigolactobacillus bifermentans

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1H332|A0A0R1H332_9LACO Uncharacterized protein OS=Lactobacillus bifermentans DSM 20003 OX=1423726 GN=FC07_GL002564 PE=4 SV=1
MM1 pKa = 7.74LDD3 pKa = 3.31EE4 pKa = 5.25NGFTRR9 pKa = 11.84PTYY12 pKa = 10.69DD13 pKa = 3.78EE14 pKa = 5.77LIQTLIQKK22 pKa = 7.83WQEE25 pKa = 3.88LFGEE29 pKa = 4.44NANVAPNSVGGIFIRR44 pKa = 11.84VLAFFLNQIYY54 pKa = 10.2QLAEE58 pKa = 3.77LVYY61 pKa = 10.19QSQFADD67 pKa = 3.44SATGTTLDD75 pKa = 3.74QLAANLGLVRR85 pKa = 11.84QAPQAAIGEE94 pKa = 4.27VQIYY98 pKa = 9.61GVAGYY103 pKa = 8.5VVPAGTLFQTDD114 pKa = 3.47DD115 pKa = 3.29GLIYY119 pKa = 9.82VTSEE123 pKa = 4.68DD124 pKa = 3.43ITLADD129 pKa = 3.53QGKK132 pKa = 7.59TSIDD136 pKa = 3.46TGDD139 pKa = 3.67LGQGVLQYY147 pKa = 10.93NDD149 pKa = 3.53KK150 pKa = 11.22NIGLGTSTVLYY161 pKa = 11.05ANGTGSNYY169 pKa = 10.29NKK171 pKa = 9.95PGVIGDD177 pKa = 4.37YY178 pKa = 10.25IAQQLMPVEE187 pKa = 4.23EE188 pKa = 4.23VLLVEE193 pKa = 4.34VGKK196 pKa = 8.85ITGGADD202 pKa = 4.36LEE204 pKa = 5.29DD205 pKa = 5.28DD206 pKa = 3.84DD207 pKa = 5.54ALRR210 pKa = 11.84DD211 pKa = 3.77RR212 pKa = 11.84LEE214 pKa = 4.09QASQEE219 pKa = 4.48APSSPYY225 pKa = 10.05NGVMSAVRR233 pKa = 11.84DD234 pKa = 3.88VVGVSSAKK242 pKa = 10.18IVVNDD247 pKa = 4.26TMDD250 pKa = 3.41TDD252 pKa = 4.46ASGNPAKK259 pKa = 9.7TLHH262 pKa = 6.57IYY264 pKa = 10.6VDD266 pKa = 4.27GGNQDD271 pKa = 4.85DD272 pKa = 3.72IGAAIFDD279 pKa = 4.66SIAAGIQTYY288 pKa = 10.64GSIEE292 pKa = 4.4VYY294 pKa = 10.73VKK296 pKa = 10.63DD297 pKa = 3.76IGGSTHH303 pKa = 6.06SVYY306 pKa = 10.8YY307 pKa = 9.74DD308 pKa = 3.28QPTAIQIFASVAATTNEE325 pKa = 3.58AFPLDD330 pKa = 4.04GNAQIQQAVVDD341 pKa = 4.26YY342 pKa = 10.16VRR344 pKa = 11.84SVGMGGTIHH353 pKa = 6.74YY354 pKa = 10.05SYY356 pKa = 10.64LYY358 pKa = 10.29KK359 pKa = 10.66YY360 pKa = 10.48LYY362 pKa = 10.97DD363 pKa = 4.84NITGLDD369 pKa = 3.54VADD372 pKa = 4.0VKK374 pKa = 11.0IGTDD378 pKa = 3.43KK379 pKa = 11.68DD380 pKa = 3.5NLTAADD386 pKa = 4.38IPLTDD391 pKa = 3.64IQRR394 pKa = 11.84ATITTDD400 pKa = 3.26TVVVAA405 pKa = 5.14

Molecular weight: 43.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1GQZ8|A0A0R1GQZ8_9LACO ABC transporter OS=Lactobacillus bifermentans DSM 20003 OX=1423726 GN=FC07_GL000823 PE=4 SV=1
MM1 pKa = 7.65HH2 pKa = 7.37YY3 pKa = 10.39IDD5 pKa = 3.64ITGQRR10 pKa = 11.84FGKK13 pKa = 10.34LVAQTCGSPDD23 pKa = 3.17RR24 pKa = 11.84QGNMLWHH31 pKa = 6.22CTCDD35 pKa = 2.94CGRR38 pKa = 11.84TLLVSGTNLRR48 pKa = 11.84QGRR51 pKa = 11.84QKK53 pKa = 11.08SCGQCGSLRR62 pKa = 11.84LIDD65 pKa = 3.6LTGQRR70 pKa = 11.84FGRR73 pKa = 11.84LVVMKK78 pKa = 10.47RR79 pKa = 11.84STQRR83 pKa = 11.84SANGNALWQCRR94 pKa = 11.84CDD96 pKa = 3.31CGKK99 pKa = 10.24QVVVDD104 pKa = 3.64SQRR107 pKa = 11.84LRR109 pKa = 11.84KK110 pKa = 9.81HH111 pKa = 4.66ITRR114 pKa = 11.84SCGCLRR120 pKa = 11.84NEE122 pKa = 4.09LAKK125 pKa = 10.54KK126 pKa = 10.42RR127 pKa = 11.84SYY129 pKa = 11.2HH130 pKa = 5.25NAAFRR135 pKa = 11.84KK136 pKa = 6.83TQGNISRR143 pKa = 11.84LKK145 pKa = 10.02DD146 pKa = 3.27ANGVFFCSTKK156 pKa = 8.27KK157 pKa = 8.96TKK159 pKa = 10.33RR160 pKa = 11.84NRR162 pKa = 11.84TGVIGVSFDD171 pKa = 3.42QHH173 pKa = 6.17SGRR176 pKa = 11.84YY177 pKa = 6.38VARR180 pKa = 11.84LRR182 pKa = 11.84YY183 pKa = 9.44RR184 pKa = 11.84GQYY187 pKa = 9.91VLNQTAATLQEE198 pKa = 4.39AATLRR203 pKa = 11.84HH204 pKa = 5.69RR205 pKa = 11.84AEE207 pKa = 3.97LKK209 pKa = 9.86YY210 pKa = 10.33FKK212 pKa = 10.48PSTTDD217 pKa = 2.65

Molecular weight: 24.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2964

0

2964

885647

46

2691

298.8

33.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.813 ± 0.078

0.629 ± 0.015

5.313 ± 0.048

4.6 ± 0.046

4.081 ± 0.036

6.719 ± 0.044

2.291 ± 0.022

6.197 ± 0.047

5.283 ± 0.056

10.509 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.025

3.999 ± 0.032

3.988 ± 0.035

6.145 ± 0.073

4.125 ± 0.047

5.114 ± 0.05

7.112 ± 0.089

7.11 ± 0.043

1.098 ± 0.018

3.388 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski