Amino acid dipepetide frequency for Mycobacterium phage Leston

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.912AlaAla: 20.912 ± 1.927
1.156AlaCys: 1.156 ± 0.253
8.197AlaAsp: 8.197 ± 0.6
7.934AlaGlu: 7.934 ± 0.909
3.258AlaPhe: 3.258 ± 0.639
13.083AlaGly: 13.083 ± 0.835
2.154AlaHis: 2.154 ± 0.354
5.254AlaIle: 5.254 ± 0.606
4.098AlaLys: 4.098 ± 0.462
10.404AlaLeu: 10.404 ± 0.864
2.837AlaMet: 2.837 ± 0.462
2.627AlaAsn: 2.627 ± 0.384
5.727AlaPro: 5.727 ± 0.676
4.519AlaGln: 4.519 ± 0.505
8.617AlaArg: 8.617 ± 0.643
5.097AlaSer: 5.097 ± 0.631
7.356AlaThr: 7.356 ± 0.789
8.827AlaVal: 8.827 ± 0.874
2.627AlaTrp: 2.627 ± 0.396
2.364AlaTyr: 2.364 ± 0.337
0.0AlaXaa: 0.0 ± 0.0
Cys
1.314CysAla: 1.314 ± 0.297
0.158CysCys: 0.158 ± 0.081
0.631CysAsp: 0.631 ± 0.187
0.631CysGlu: 0.631 ± 0.237
0.105CysPhe: 0.105 ± 0.069
1.576CysGly: 1.576 ± 0.401
0.368CysHis: 0.368 ± 0.165
0.42CysIle: 0.42 ± 0.151
0.42CysLys: 0.42 ± 0.135
0.525CysLeu: 0.525 ± 0.174
0.105CysMet: 0.105 ± 0.077
0.263CysAsn: 0.263 ± 0.119
0.631CysPro: 0.631 ± 0.205
0.263CysGln: 0.263 ± 0.121
0.42CysArg: 0.42 ± 0.156
0.736CysSer: 0.736 ± 0.248
0.631CysThr: 0.631 ± 0.211
0.893CysVal: 0.893 ± 0.259
0.368CysTrp: 0.368 ± 0.124
0.21CysTyr: 0.21 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
8.67AspAla: 8.67 ± 0.77
1.103AspCys: 1.103 ± 0.294
5.885AspAsp: 5.885 ± 0.573
5.517AspGlu: 5.517 ± 0.651
1.261AspPhe: 1.261 ± 0.295
6.463AspGly: 6.463 ± 0.646
1.156AspHis: 1.156 ± 0.233
2.049AspIle: 2.049 ± 0.266
2.207AspLys: 2.207 ± 0.335
6.042AspLeu: 6.042 ± 0.562
1.261AspMet: 1.261 ± 0.314
1.524AspAsn: 1.524 ± 0.294
3.625AspPro: 3.625 ± 0.468
1.314AspGln: 1.314 ± 0.243
4.939AspArg: 4.939 ± 0.494
1.576AspSer: 1.576 ± 0.229
4.466AspThr: 4.466 ± 0.428
5.097AspVal: 5.097 ± 0.467
0.893AspTrp: 0.893 ± 0.186
1.471AspTyr: 1.471 ± 0.226
0.0AspXaa: 0.0 ± 0.0
Glu
7.198GluAla: 7.198 ± 0.76
0.525GluCys: 0.525 ± 0.151
3.678GluAsp: 3.678 ± 0.433
2.785GluGlu: 2.785 ± 0.381
1.944GluPhe: 1.944 ± 0.397
3.783GluGly: 3.783 ± 0.471
1.786GluHis: 1.786 ± 0.307
1.524GluIle: 1.524 ± 0.337
1.629GluLys: 1.629 ± 0.312
5.622GluLeu: 5.622 ± 0.556
1.576GluMet: 1.576 ± 0.274
1.051GluAsn: 1.051 ± 0.222
3.205GluPro: 3.205 ± 0.481
2.837GluGln: 2.837 ± 0.478
4.887GluArg: 4.887 ± 0.829
2.732GluSer: 2.732 ± 0.375
2.522GluThr: 2.522 ± 0.417
4.519GluVal: 4.519 ± 0.525
1.208GluTrp: 1.208 ± 0.207
1.419GluTyr: 1.419 ± 0.25
0.0GluXaa: 0.0 ± 0.0
Phe
3.047PheAla: 3.047 ± 0.458
0.21PheCys: 0.21 ± 0.108
2.785PheAsp: 2.785 ± 0.366
1.681PheGlu: 1.681 ± 0.268
0.631PhePhe: 0.631 ± 0.163
2.837PheGly: 2.837 ± 0.336
0.473PheHis: 0.473 ± 0.145
1.524PheIle: 1.524 ± 0.244
1.103PheLys: 1.103 ± 0.273
1.681PheLeu: 1.681 ± 0.34
0.578PheMet: 0.578 ± 0.191
0.998PheAsn: 0.998 ± 0.268
1.366PhePro: 1.366 ± 0.254
0.683PheGln: 0.683 ± 0.196
1.524PheArg: 1.524 ± 0.251
1.051PheSer: 1.051 ± 0.288
2.102PheThr: 2.102 ± 0.34
1.734PheVal: 1.734 ± 0.333
0.42PheTrp: 0.42 ± 0.155
0.578PheTyr: 0.578 ± 0.157
0.0PheXaa: 0.0 ± 0.0
Gly
9.72GlyAla: 9.72 ± 0.928
1.051GlyCys: 1.051 ± 0.261
5.832GlyAsp: 5.832 ± 0.61
5.359GlyGlu: 5.359 ± 0.472
2.522GlyPhe: 2.522 ± 0.42
9.037GlyGly: 9.037 ± 1.201
1.734GlyHis: 1.734 ± 0.365
3.836GlyIle: 3.836 ± 0.427
3.363GlyLys: 3.363 ± 0.354
5.675GlyLeu: 5.675 ± 0.647
1.892GlyMet: 1.892 ± 0.372
3.31GlyAsn: 3.31 ± 0.495
4.098GlyPro: 4.098 ± 0.534
2.68GlyGln: 2.68 ± 0.425
6.778GlyArg: 6.778 ± 0.585
4.309GlySer: 4.309 ± 0.484
6.62GlyThr: 6.62 ± 0.616
6.358GlyVal: 6.358 ± 0.512
1.734GlyTrp: 1.734 ± 0.249
1.892GlyTyr: 1.892 ± 0.374
0.0GlyXaa: 0.0 ± 0.0
His
2.417HisAla: 2.417 ± 0.329
0.368HisCys: 0.368 ± 0.127
1.314HisAsp: 1.314 ± 0.275
0.998HisGlu: 0.998 ± 0.223
0.473HisPhe: 0.473 ± 0.17
2.102HisGly: 2.102 ± 0.411
0.631HisHis: 0.631 ± 0.175
0.998HisIle: 0.998 ± 0.282
0.736HisLys: 0.736 ± 0.186
2.312HisLeu: 2.312 ± 0.296
0.525HisMet: 0.525 ± 0.152
0.631HisAsn: 0.631 ± 0.158
1.366HisPro: 1.366 ± 0.278
0.315HisGln: 0.315 ± 0.116
1.786HisArg: 1.786 ± 0.315
0.946HisSer: 0.946 ± 0.194
1.156HisThr: 1.156 ± 0.24
1.786HisVal: 1.786 ± 0.347
0.473HisTrp: 0.473 ± 0.204
0.578HisTyr: 0.578 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
5.044IleAla: 5.044 ± 0.502
0.263IleCys: 0.263 ± 0.173
3.415IleAsp: 3.415 ± 0.443
3.363IleGlu: 3.363 ± 0.483
0.631IlePhe: 0.631 ± 0.164
4.151IleGly: 4.151 ± 0.559
0.473IleHis: 0.473 ± 0.154
1.471IleIle: 1.471 ± 0.261
1.629IleLys: 1.629 ± 0.364
2.417IleLeu: 2.417 ± 0.304
0.631IleMet: 0.631 ± 0.202
1.786IleAsn: 1.786 ± 0.377
2.312IlePro: 2.312 ± 0.517
1.103IleGln: 1.103 ± 0.221
2.575IleArg: 2.575 ± 0.374
1.734IleSer: 1.734 ± 0.311
2.575IleThr: 2.575 ± 0.306
3.888IleVal: 3.888 ± 0.472
0.683IleTrp: 0.683 ± 0.284
0.736IleTyr: 0.736 ± 0.167
0.0IleXaa: 0.0 ± 0.0
Lys
4.151LysAla: 4.151 ± 0.515
0.315LysCys: 0.315 ± 0.132
0.683LysAsp: 0.683 ± 0.213
0.998LysGlu: 0.998 ± 0.176
1.261LysPhe: 1.261 ± 0.233
1.839LysGly: 1.839 ± 0.354
0.893LysHis: 0.893 ± 0.25
1.261LysIle: 1.261 ± 0.297
1.156LysLys: 1.156 ± 0.229
3.731LysLeu: 3.731 ± 0.462
0.893LysMet: 0.893 ± 0.229
1.051LysAsn: 1.051 ± 0.274
3.047LysPro: 3.047 ± 0.458
1.839LysGln: 1.839 ± 0.348
3.153LysArg: 3.153 ± 0.358
1.681LysSer: 1.681 ± 0.299
2.207LysThr: 2.207 ± 0.314
2.575LysVal: 2.575 ± 0.363
0.736LysTrp: 0.736 ± 0.202
0.841LysTyr: 0.841 ± 0.197
0.0LysXaa: 0.0 ± 0.0
Leu
10.561LeuAla: 10.561 ± 0.621
1.103LeuCys: 1.103 ± 0.273
6.305LeuAsp: 6.305 ± 0.606
2.995LeuGlu: 2.995 ± 0.351
2.312LeuPhe: 2.312 ± 0.37
6.148LeuGly: 6.148 ± 0.75
1.944LeuHis: 1.944 ± 0.33
3.205LeuIle: 3.205 ± 0.346
3.258LeuLys: 3.258 ± 0.464
5.517LeuLeu: 5.517 ± 0.559
1.576LeuMet: 1.576 ± 0.33
2.259LeuAsn: 2.259 ± 0.309
4.519LeuPro: 4.519 ± 0.535
3.31LeuGln: 3.31 ± 0.446
6.568LeuArg: 6.568 ± 0.695
4.571LeuSer: 4.571 ± 0.556
5.675LeuThr: 5.675 ± 0.573
5.044LeuVal: 5.044 ± 0.521
1.524LeuTrp: 1.524 ± 0.204
1.524LeuTyr: 1.524 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
2.207MetAla: 2.207 ± 0.256
0.158MetCys: 0.158 ± 0.083
0.736MetAsp: 0.736 ± 0.193
0.683MetGlu: 0.683 ± 0.17
0.788MetPhe: 0.788 ± 0.224
1.576MetGly: 1.576 ± 0.295
0.473MetHis: 0.473 ± 0.175
0.736MetIle: 0.736 ± 0.201
0.578MetLys: 0.578 ± 0.216
2.364MetLeu: 2.364 ± 0.365
0.42MetMet: 0.42 ± 0.186
0.736MetAsn: 0.736 ± 0.179
1.366MetPro: 1.366 ± 0.263
0.788MetGln: 0.788 ± 0.265
1.366MetArg: 1.366 ± 0.267
1.944MetSer: 1.944 ± 0.384
2.417MetThr: 2.417 ± 0.327
1.366MetVal: 1.366 ± 0.235
0.368MetTrp: 0.368 ± 0.113
0.473MetTyr: 0.473 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
3.941AsnAla: 3.941 ± 0.659
0.263AsnCys: 0.263 ± 0.104
1.681AsnAsp: 1.681 ± 0.277
0.946AsnGlu: 0.946 ± 0.223
0.525AsnPhe: 0.525 ± 0.248
3.573AsnGly: 3.573 ± 0.338
0.578AsnHis: 0.578 ± 0.188
0.893AsnIle: 0.893 ± 0.23
0.893AsnLys: 0.893 ± 0.208
2.259AsnLeu: 2.259 ± 0.405
0.683AsnMet: 0.683 ± 0.138
0.736AsnAsn: 0.736 ± 0.178
2.68AsnPro: 2.68 ± 0.368
0.841AsnGln: 0.841 ± 0.206
1.944AsnArg: 1.944 ± 0.33
0.998AsnSer: 0.998 ± 0.256
1.997AsnThr: 1.997 ± 0.323
2.364AsnVal: 2.364 ± 0.298
0.631AsnTrp: 0.631 ± 0.167
0.525AsnTyr: 0.525 ± 0.166
0.0AsnXaa: 0.0 ± 0.0
Pro
8.565ProAla: 8.565 ± 0.68
0.315ProCys: 0.315 ± 0.145
3.888ProAsp: 3.888 ± 0.534
3.415ProGlu: 3.415 ± 0.323
1.366ProPhe: 1.366 ± 0.248
4.781ProGly: 4.781 ± 0.577
1.314ProHis: 1.314 ± 0.264
2.154ProIle: 2.154 ± 0.274
2.312ProLys: 2.312 ± 0.32
3.993ProLeu: 3.993 ± 0.537
1.208ProMet: 1.208 ± 0.244
1.576ProAsn: 1.576 ± 0.275
3.153ProPro: 3.153 ± 0.462
2.364ProGln: 2.364 ± 0.441
3.363ProArg: 3.363 ± 0.398
2.68ProSer: 2.68 ± 0.434
3.31ProThr: 3.31 ± 0.365
4.571ProVal: 4.571 ± 0.526
0.788ProTrp: 0.788 ± 0.189
1.314ProTyr: 1.314 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
4.466GlnAla: 4.466 ± 0.495
0.21GlnCys: 0.21 ± 0.106
1.208GlnAsp: 1.208 ± 0.269
1.524GlnGlu: 1.524 ± 0.274
0.893GlnPhe: 0.893 ± 0.218
1.629GlnGly: 1.629 ± 0.268
0.788GlnHis: 0.788 ± 0.244
1.892GlnIle: 1.892 ± 0.302
1.156GlnLys: 1.156 ± 0.22
2.942GlnLeu: 2.942 ± 0.455
0.998GlnMet: 0.998 ± 0.23
0.946GlnAsn: 0.946 ± 0.215
2.522GlnPro: 2.522 ± 0.412
1.261GlnGln: 1.261 ± 0.258
3.047GlnArg: 3.047 ± 0.435
1.786GlnSer: 1.786 ± 0.336
2.102GlnThr: 2.102 ± 0.201
2.942GlnVal: 2.942 ± 0.375
0.998GlnTrp: 0.998 ± 0.225
0.893GlnTyr: 0.893 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
7.776ArgAla: 7.776 ± 0.844
1.261ArgCys: 1.261 ± 0.268
4.466ArgAsp: 4.466 ± 0.488
4.203ArgGlu: 4.203 ± 0.437
1.997ArgPhe: 1.997 ± 0.265
5.727ArgGly: 5.727 ± 0.571
1.786ArgHis: 1.786 ± 0.304
4.046ArgIle: 4.046 ± 0.372
3.205ArgLys: 3.205 ± 0.46
6.358ArgLeu: 6.358 ± 0.654
1.892ArgMet: 1.892 ± 0.335
1.892ArgAsn: 1.892 ± 0.305
3.468ArgPro: 3.468 ± 0.428
3.1ArgGln: 3.1 ± 0.403
6.463ArgArg: 6.463 ± 0.833
3.625ArgSer: 3.625 ± 0.549
4.571ArgThr: 4.571 ± 0.557
4.729ArgVal: 4.729 ± 0.544
1.471ArgTrp: 1.471 ± 0.262
1.734ArgTyr: 1.734 ± 0.273
0.0ArgXaa: 0.0 ± 0.0
Ser
5.727SerAla: 5.727 ± 0.754
0.42SerCys: 0.42 ± 0.164
3.31SerAsp: 3.31 ± 0.386
2.627SerGlu: 2.627 ± 0.457
1.051SerPhe: 1.051 ± 0.207
4.571SerGly: 4.571 ± 0.726
0.841SerHis: 0.841 ± 0.235
2.312SerIle: 2.312 ± 0.459
1.366SerLys: 1.366 ± 0.276
2.417SerLeu: 2.417 ± 0.356
1.471SerMet: 1.471 ± 0.231
1.944SerAsn: 1.944 ± 0.333
2.049SerPro: 2.049 ± 0.296
1.314SerGln: 1.314 ± 0.312
2.47SerArg: 2.47 ± 0.324
3.153SerSer: 3.153 ± 0.549
4.046SerThr: 4.046 ± 0.351
4.414SerVal: 4.414 ± 0.636
1.366SerTrp: 1.366 ± 0.274
1.156SerTyr: 1.156 ± 0.208
0.0SerXaa: 0.0 ± 0.0
Thr
8.039ThrAla: 8.039 ± 0.786
0.631ThrCys: 0.631 ± 0.243
3.888ThrAsp: 3.888 ± 0.478
3.888ThrGlu: 3.888 ± 0.509
2.207ThrPhe: 2.207 ± 0.291
6.042ThrGly: 6.042 ± 0.633
1.629ThrHis: 1.629 ± 0.333
3.205ThrIle: 3.205 ± 0.312
1.524ThrLys: 1.524 ± 0.246
5.622ThrLeu: 5.622 ± 0.626
0.998ThrMet: 0.998 ± 0.259
1.839ThrAsn: 1.839 ± 0.278
4.571ThrPro: 4.571 ± 0.412
1.786ThrGln: 1.786 ± 0.254
4.414ThrArg: 4.414 ± 0.537
2.89ThrSer: 2.89 ± 0.456
3.888ThrThr: 3.888 ± 0.559
6.515ThrVal: 6.515 ± 0.506
1.314ThrTrp: 1.314 ± 0.323
1.681ThrTyr: 1.681 ± 0.304
0.0ThrXaa: 0.0 ± 0.0
Val
8.932ValAla: 8.932 ± 0.902
0.683ValCys: 0.683 ± 0.218
6.148ValAsp: 6.148 ± 0.607
4.571ValGlu: 4.571 ± 0.493
2.417ValPhe: 2.417 ± 0.436
5.885ValGly: 5.885 ± 0.547
1.997ValHis: 1.997 ± 0.272
2.522ValIle: 2.522 ± 0.387
2.732ValLys: 2.732 ± 0.431
6.095ValLeu: 6.095 ± 0.567
1.314ValMet: 1.314 ± 0.243
2.47ValAsn: 2.47 ± 0.284
4.414ValPro: 4.414 ± 0.504
1.997ValGln: 1.997 ± 0.32
5.727ValArg: 5.727 ± 0.673
3.941ValSer: 3.941 ± 0.504
5.78ValThr: 5.78 ± 0.648
6.148ValVal: 6.148 ± 0.603
1.261ValTrp: 1.261 ± 0.21
1.524ValTyr: 1.524 ± 0.273
0.0ValXaa: 0.0 ± 0.0
Trp
1.944TrpAla: 1.944 ± 0.354
0.315TrpCys: 0.315 ± 0.14
1.156TrpAsp: 1.156 ± 0.311
0.998TrpGlu: 0.998 ± 0.248
0.683TrpPhe: 0.683 ± 0.185
0.998TrpGly: 0.998 ± 0.253
0.473TrpHis: 0.473 ± 0.167
0.683TrpIle: 0.683 ± 0.219
0.473TrpLys: 0.473 ± 0.155
2.154TrpLeu: 2.154 ± 0.326
0.315TrpMet: 0.315 ± 0.133
0.788TrpAsn: 0.788 ± 0.155
1.156TrpPro: 1.156 ± 0.26
0.946TrpGln: 0.946 ± 0.222
1.734TrpArg: 1.734 ± 0.307
1.103TrpSer: 1.103 ± 0.212
1.471TrpThr: 1.471 ± 0.247
1.314TrpVal: 1.314 ± 0.299
0.21TrpTrp: 0.21 ± 0.118
0.473TrpTyr: 0.473 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.417TyrAla: 2.417 ± 0.339
0.158TyrCys: 0.158 ± 0.088
1.314TyrAsp: 1.314 ± 0.288
1.314TyrGlu: 1.314 ± 0.299
0.683TyrPhe: 0.683 ± 0.163
1.997TyrGly: 1.997 ± 0.337
0.42TyrHis: 0.42 ± 0.126
0.893TyrIle: 0.893 ± 0.251
0.525TyrLys: 0.525 ± 0.162
1.786TyrLeu: 1.786 ± 0.324
0.315TyrMet: 0.315 ± 0.129
0.525TyrAsn: 0.525 ± 0.146
1.156TyrPro: 1.156 ± 0.241
0.946TyrGln: 0.946 ± 0.217
1.997TyrArg: 1.997 ± 0.329
1.471TyrSer: 1.471 ± 0.226
1.681TyrThr: 1.681 ± 0.342
1.471TyrVal: 1.471 ± 0.277
0.315TyrTrp: 0.315 ± 0.111
0.683TyrTyr: 0.683 ± 0.214
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (19033 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski