Capybara microvirus Cap3_SP_562

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W8W4|A0A4P8W8W4_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_562 OX=2585476 PE=4 SV=1
MM1 pKa = 7.56YY2 pKa = 9.84DD3 pKa = 3.17VKK5 pKa = 11.38VYY7 pKa = 9.66VYY9 pKa = 9.69CLRR12 pKa = 11.84EE13 pKa = 3.73ASFSNDD19 pKa = 2.55SKK21 pKa = 11.76SNYY24 pKa = 8.67EE25 pKa = 3.71IKK27 pKa = 10.57ACYY30 pKa = 10.01DD31 pKa = 3.31HH32 pKa = 6.8VSCYY36 pKa = 10.5SVDD39 pKa = 3.29MYY41 pKa = 11.8YY42 pKa = 11.06SLANNLGSLRR52 pKa = 11.84LVDD55 pKa = 5.35RR56 pKa = 11.84FATTPNLDD64 pKa = 4.27SIDD67 pKa = 5.0LYY69 pKa = 10.47IDD71 pKa = 3.91CYY73 pKa = 10.82FADD76 pKa = 5.37FDD78 pKa = 4.14NTCCSMYY85 pKa = 10.5IRR87 pKa = 11.84MLILEE92 pKa = 4.57NNFDD96 pKa = 3.96TLIKK100 pKa = 10.5LFRR103 pKa = 11.84NGLCC107 pKa = 3.9

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W665|A0A4P8W665_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_562 OX=2585476 PE=4 SV=1
MM1 pKa = 7.51CVNPKK6 pKa = 10.35FGFLVTDD13 pKa = 3.9SFRR16 pKa = 11.84SGLIKK21 pKa = 10.46RR22 pKa = 11.84SVHH25 pKa = 5.37FCTKK29 pKa = 7.75EE30 pKa = 3.47HH31 pKa = 5.77YY32 pKa = 10.66LYY34 pKa = 10.3MLEE37 pKa = 5.16NVDD40 pKa = 3.29IYY42 pKa = 11.25KK43 pKa = 10.36NEE45 pKa = 3.93YY46 pKa = 9.4HH47 pKa = 6.41SEE49 pKa = 3.9PMTFPCGRR57 pKa = 11.84CPEE60 pKa = 4.45CCMEE64 pKa = 5.65HH65 pKa = 7.0ADD67 pKa = 3.21AWAYY71 pKa = 10.91RR72 pKa = 11.84IMNEE76 pKa = 3.99ASQYY80 pKa = 11.39DD81 pKa = 4.28DD82 pKa = 3.51NCFITLTYY90 pKa = 11.26ANTDD94 pKa = 2.91NVNYY98 pKa = 10.67DD99 pKa = 3.31NASLQKK105 pKa = 10.45KK106 pKa = 9.67DD107 pKa = 3.22VQDD110 pKa = 3.56FVKK113 pKa = 10.54RR114 pKa = 11.84LRR116 pKa = 11.84EE117 pKa = 3.97RR118 pKa = 11.84IRR120 pKa = 11.84RR121 pKa = 11.84ASGKK125 pKa = 9.97KK126 pKa = 9.32IRR128 pKa = 11.84FFCCGEE134 pKa = 3.96YY135 pKa = 10.82GEE137 pKa = 4.83KK138 pKa = 9.94KK139 pKa = 9.89QRR141 pKa = 11.84PHH143 pKa = 4.85YY144 pKa = 10.13HH145 pKa = 6.7IIIFGWKK152 pKa = 9.73PNDD155 pKa = 3.22LKK157 pKa = 11.26YY158 pKa = 11.03FKK160 pKa = 9.84TINKK164 pKa = 8.9HH165 pKa = 4.38IVYY168 pKa = 9.06TSEE171 pKa = 3.93FVRR174 pKa = 11.84DD175 pKa = 3.23VWKK178 pKa = 9.91WGFITVSEE186 pKa = 4.59VEE188 pKa = 4.89LEE190 pKa = 4.23TALYY194 pKa = 9.67SALYY198 pKa = 7.79MQKK201 pKa = 10.6LLPKK205 pKa = 9.88QVGKK209 pKa = 10.39SEE211 pKa = 4.25PFLLMSTNPGIGYY224 pKa = 9.35NFLINNIDD232 pKa = 3.71NLILTDD238 pKa = 4.18KK239 pKa = 10.66IIVNGHH245 pKa = 4.31TCKK248 pKa = 10.59LPRR251 pKa = 11.84YY252 pKa = 7.36YY253 pKa = 10.95LRR255 pKa = 11.84VLEE258 pKa = 5.52DD259 pKa = 4.03KK260 pKa = 11.06LLLDD264 pKa = 4.01TSTLRR269 pKa = 11.84KK270 pKa = 9.31NRR272 pKa = 11.84SIKK275 pKa = 10.55SEE277 pKa = 3.98LAASSLDD284 pKa = 3.42FSGKK288 pKa = 9.43IEE290 pKa = 4.23IYY292 pKa = 10.18KK293 pKa = 10.42KK294 pKa = 10.04LYY296 pKa = 9.96NWFIPDD302 pKa = 3.31TT303 pKa = 4.14

Molecular weight: 35.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1490

78

551

212.9

24.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.577 ± 1.024

1.812 ± 0.707

6.51 ± 0.797

4.631 ± 0.688

5.302 ± 0.4

5.839 ± 0.963

1.477 ± 0.456

6.711 ± 0.624

6.309 ± 1.164

9.128 ± 0.986

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.812 ± 0.245

7.047 ± 0.701

3.624 ± 1.332

3.826 ± 0.761

3.893 ± 0.547

7.651 ± 0.765

6.107 ± 0.636

4.966 ± 0.556

1.007 ± 0.272

5.772 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski