Pyramidobacter piscolens W5455

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Pyramidobacter; Pyramidobacter piscolens

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1Y3X8|D1Y3X8_9BACT Pyridine nucleotide-disulfide oxidoreductase OS=Pyramidobacter piscolens W5455 OX=352165 GN=HMPREF7215_2470 PE=4 SV=1
MM1 pKa = 7.59SARR4 pKa = 11.84EE5 pKa = 3.8KK6 pKa = 10.14IAYY9 pKa = 9.22IKK11 pKa = 10.92GLLDD15 pKa = 3.74AGKK18 pKa = 9.67PEE20 pKa = 4.65DD21 pKa = 4.5KK22 pKa = 10.76LALSLFDD29 pKa = 4.42AVVEE33 pKa = 4.3ALDD36 pKa = 4.6ALADD40 pKa = 3.91EE41 pKa = 4.73NGEE44 pKa = 4.05LKK46 pKa = 10.56KK47 pKa = 10.48QLEE50 pKa = 4.64EE51 pKa = 3.84QRR53 pKa = 11.84SAADD57 pKa = 4.0DD58 pKa = 4.29LYY60 pKa = 11.13SICGEE65 pKa = 4.14LDD67 pKa = 3.23ADD69 pKa = 4.1LSDD72 pKa = 3.52VEE74 pKa = 4.6SRR76 pKa = 11.84LGIDD80 pKa = 3.72GEE82 pKa = 4.21LDD84 pKa = 3.2EE85 pKa = 6.97DD86 pKa = 4.13EE87 pKa = 5.97ASLEE91 pKa = 3.96DD92 pKa = 5.19DD93 pKa = 4.05YY94 pKa = 12.14TEE96 pKa = 4.43VACPSCGLHH105 pKa = 6.78FYY107 pKa = 10.26CQTSMLSPDD116 pKa = 3.97DD117 pKa = 4.21HH118 pKa = 8.5CVEE121 pKa = 5.23CPDD124 pKa = 4.12CGEE127 pKa = 4.26KK128 pKa = 10.96VSVDD132 pKa = 4.37LEE134 pKa = 5.0AEE136 pKa = 4.12DD137 pKa = 4.9HH138 pKa = 7.28DD139 pKa = 5.72ADD141 pKa = 3.94

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1Y0V6|D1Y0V6_9BACT LysR substrate binding domain protein OS=Pyramidobacter piscolens W5455 OX=352165 GN=HMPREF7215_2504 PE=3 SV=1
MM1 pKa = 7.26KK2 pKa = 9.47QTFQPHH8 pKa = 3.62VRR10 pKa = 11.84SRR12 pKa = 11.84KK13 pKa = 9.16RR14 pKa = 11.84GMGFLARR21 pKa = 11.84SRR23 pKa = 11.84SHH25 pKa = 6.61GGRR28 pKa = 11.84GVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2755

0

2755

764429

31

2481

277.5

30.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.869 ± 0.067

1.537 ± 0.023

5.163 ± 0.037

6.322 ± 0.053

4.097 ± 0.037

8.351 ± 0.056

1.702 ± 0.021

5.274 ± 0.042

4.86 ± 0.04

10.135 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.871 ± 0.024

3.061 ± 0.034

4.437 ± 0.029

2.7 ± 0.023

6.665 ± 0.058

5.676 ± 0.034

4.659 ± 0.033

7.595 ± 0.038

1.207 ± 0.02

2.82 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski