Staphylococcus phage 44AHJD

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Rakietenvirinae; Rosenblumvirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q859M3|Q859M3_BP44A Uncharacterized protein OS=Staphylococcus phage 44AHJD OX=204086 PE=4 SV=1
MM1 pKa = 7.27VNVDD5 pKa = 3.72NAPEE9 pKa = 4.15EE10 pKa = 4.21KK11 pKa = 10.3GQAYY15 pKa = 8.38TEE17 pKa = 3.92MLQLFNKK24 pKa = 9.67LIQWNPAYY32 pKa = 9.81TFDD35 pKa = 4.42NAINLLSACQQLLLNYY51 pKa = 9.4NSSVVQFLNDD61 pKa = 3.39EE62 pKa = 4.81LNNEE66 pKa = 4.41TKK68 pKa = 10.41PEE70 pKa = 4.48SILSYY75 pKa = 10.49IAGDD79 pKa = 4.29DD80 pKa = 5.37PIEE83 pKa = 3.81QWNMHH88 pKa = 5.77KK89 pKa = 10.66GFYY92 pKa = 7.63EE93 pKa = 4.27TYY95 pKa = 9.67NVYY98 pKa = 10.34VFF100 pKa = 4.47

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q859L1|Q859L1_BP44A Tail protein OS=Staphylococcus phage 44AHJD OX=204086 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.52MVHH5 pKa = 5.98LHH7 pKa = 4.78VVFYY11 pKa = 10.6QYY13 pKa = 11.58LHH15 pKa = 6.18VSVVQNYY22 pKa = 7.79QNLMAIGSNQTVIHH36 pKa = 7.04HH37 pKa = 5.56ITKK40 pKa = 10.08FVYY43 pKa = 10.45QMVTYY48 pKa = 10.52GLVITGKK55 pKa = 9.46AHH57 pKa = 6.27VIIYY61 pKa = 9.56QCANGMEE68 pKa = 4.28KK69 pKa = 10.3QVIVTVLVFLGGCSHH84 pKa = 6.95NGYY87 pKa = 10.28FSLFLL92 pKa = 5.21

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

5203

55

755

247.8

28.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.978 ± 0.494

0.634 ± 0.154

7.323 ± 0.36

6.342 ± 0.663

5.651 ± 0.297

5.016 ± 0.845

1.98 ± 0.243

7.284 ± 0.691

8.034 ± 0.325

8.188 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.269

8.322 ± 0.471

2.556 ± 0.338

3.748 ± 0.433

3.267 ± 0.333

6.381 ± 0.403

5.92 ± 0.348

5.324 ± 0.373

0.98 ± 0.259

6.304 ± 0.492
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski