Paenibacillus phage vB_PlaP_API480

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8EHK9|A0A5B8EHK9_9CAUD Uncharacterized protein OS=Paenibacillus phage vB_PlaP_API480 OX=2530020 GN=API480_16 PE=4 SV=1
MM1 pKa = 7.16NVQVIGVIEE10 pKa = 3.91QDD12 pKa = 4.9GYY14 pKa = 10.95ILWAEE19 pKa = 4.12DD20 pKa = 3.46LGVEE24 pKa = 4.1PEE26 pKa = 4.3FYY28 pKa = 10.66GVYY31 pKa = 9.83AQKK34 pKa = 11.0EE35 pKa = 4.18DD36 pKa = 3.8GTYY39 pKa = 10.06EE40 pKa = 3.89WLGDD44 pKa = 3.64FQNISDD50 pKa = 3.74AMRR53 pKa = 11.84YY54 pKa = 9.87LGVKK58 pKa = 10.08NFF60 pKa = 3.66

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B7LZC4|A0A5B7LZC4_9CAUD DUF1599 domain-containing protein OS=Paenibacillus phage vB_PlaP_API480 OX=2530020 GN=API480_3 PE=4 SV=1
MM1 pKa = 6.87KK2 pKa = 10.0TVVLYY7 pKa = 10.72NGRR10 pKa = 11.84TYY12 pKa = 11.12KK13 pKa = 10.48RR14 pKa = 11.84LKK16 pKa = 10.74DD17 pKa = 3.83GMHH20 pKa = 5.98GRR22 pKa = 11.84AFPSNIAGKK31 pKa = 9.86LCRR34 pKa = 11.84QPGCLPYY41 pKa = 10.08IYY43 pKa = 10.01ILVGKK48 pKa = 9.11CLHH51 pKa = 7.09LARR54 pKa = 11.84KK55 pKa = 8.23MVGQRR60 pKa = 3.17

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

13794

39

1631

179.1

20.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.264 ± 0.471

1.131 ± 0.189

6.438 ± 0.198

8.591 ± 0.432

3.893 ± 0.175

6.264 ± 0.257

1.856 ± 0.141

6.713 ± 0.396

8.656 ± 0.544

8.641 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.871 ± 0.127

4.756 ± 0.215

3.299 ± 0.215

3.741 ± 0.264

4.683 ± 0.192

5.502 ± 0.186

4.785 ± 0.193

6.09 ± 0.399

1.167 ± 0.137

4.661 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski